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The map label for this gene is capL [H]

Identifier: 222150925

GI number: 222150925

Start: 728353

End: 729627

Strand: Direct

Name: capL [H]

Synonym: MCCL_0675

Alternate gene names: 222150925

Gene position: 728353-729627 (Clockwise)

Preceding gene: 222150924

Following gene: 222150926

Centisome position: 34.65

GC content: 32.31

Gene sequence:

>1275_bases
ATGAATAGAAAAATCGCAGTAGTAGGATTAGGTTATGTAGGTTTACCCGTAGCAGCTGCATTTGGAAAATTACATGAAGT
TATAGGATTCGATATTAATGAATCAAGAATCCAAGAATTAAAAGACGGATATGATAGAACAAATGAAGTGAAATCTGAAG
ATTTAGCTGCTACAAAAATAAATTTTACTTCTAACAAATCAGATTTAGCCAAAGCAGATTTTATTATTGTTGCTGTACCT
ACACCAATTAATAAAAATAATCAACCTGATTTGACACCCCTTATTAAAGCGAGTGAGACTGTTGGTTCTGCGATGGAAAA
AGGTACAATCGTAGTTTACGAATCAACTGTTTATCCTGGAGCTACAAGAGAAGATTGTGTACCAGTATTAGAACAATTCT
CAGGTTTAAAATGTGGAGAAGACTTCTTTGTTGGTTATTCACCTGAACGTATTAATCCTGGTGATAAAGTACATACATTT
GAAACAATAACAAAAGTTGTGAGTGGGCAAACCCCAGAAGTATTAGAGATTGTAGCCGACGTCTATGGTTCTGTTGTAAA
AGCAGGTGTACATAAAGCACCAACAATTGAGGTTGCTGAAGCTGCTAAAGTTATTGAAAATACTCAACGAGATATAAACA
TCGCTTTAATGAATGAACTTGCATTAATTTTTGATAAAGCAGGGATTAATACGAAAGATGTGCTTGAAGCCGCAGGAACG
AAATGGAACTTCTTAAAGTTCACTCCTGGTTTAGTAGGTGGACATTGTATTGGTGTTGATCCATACTACTTAACACATAA
AGCTCAAGAATTAGGACATCATCCTGAAGTTATTTTATCCGGACGCCGTATTAATGATGGCATGAGCAAGTATATTGCTT
CTAAGATTGTAAAAGAAATGCTGAGCAAAGGATTGACTGTTAATAAATCAAATGTAAATGTATTAGGCTTAACATTTAAG
GAAAATTGTCCAGACTTAAGAAATACAAAAGTAATTGATATCATAAGAGAACTTGAGGAATATGGAATTACTGTAAATGT
ACACGATGCAAAAGCGGATAAAGAAGAAGCTAAAAAGTTTTATAATATTGAATTAAAAGAGGTAAATGAACTTCCTGAAA
CAGATTTAGTTTTATTTGCAGTTTCTCATAATGAATATATTCAAAACAAGGAAGAGTTTCTAAAAAAATTATCGAATAAT
GGGATTGTATTTGATATTAAAGGTATTATTAATAAAGAAGATACCTCAAATAACCAAACAATTTGGAGTTTATAA

Upstream 100 bases:

>100_bases
TTTCAAACCACAAACGAACATTCAAGATGGAGTTAATAATTTTGTTGATTGGTATATGAATTATTACGATATTAAAAAAT
AATTTTAAAGGATGTATATA

Downstream 100 bases:

>100_bases
TAAAAGGAGCTTTTATGAAAATTATACAACTTTCTGCAATTGATATGACAATGAACAATTTTTTGAGACCATTAAATATT
GCTACTCGTGATGCTGGTTT

Product: capsular polysaccharide biosynthesis protein CapL

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNRKIAVVGLGYVGLPVAAAFGKLHEVIGFDINESRIQELKDGYDRTNEVKSEDLAATKINFTSNKSDLAKADFIIVAVP
TPINKNNQPDLTPLIKASETVGSAMEKGTIVVYESTVYPGATREDCVPVLEQFSGLKCGEDFFVGYSPERINPGDKVHTF
ETITKVVSGQTPEVLEIVADVYGSVVKAGVHKAPTIEVAEAAKVIENTQRDINIALMNELALIFDKAGINTKDVLEAAGT
KWNFLKFTPGLVGGHCIGVDPYYLTHKAQELGHHPEVILSGRRINDGMSKYIASKIVKEMLSKGLTVNKSNVNVLGLTFK
ENCPDLRNTKVIDIIRELEEYGITVNVHDAKADKEEAKKFYNIELKEVNELPETDLVLFAVSHNEYIQNKEEFLKKLSNN
GIVFDIKGIINKEDTSNNQTIWSL

Sequences:

>Translated_424_residues
MNRKIAVVGLGYVGLPVAAAFGKLHEVIGFDINESRIQELKDGYDRTNEVKSEDLAATKINFTSNKSDLAKADFIIVAVP
TPINKNNQPDLTPLIKASETVGSAMEKGTIVVYESTVYPGATREDCVPVLEQFSGLKCGEDFFVGYSPERINPGDKVHTF
ETITKVVSGQTPEVLEIVADVYGSVVKAGVHKAPTIEVAEAAKVIENTQRDINIALMNELALIFDKAGINTKDVLEAAGT
KWNFLKFTPGLVGGHCIGVDPYYLTHKAQELGHHPEVILSGRRINDGMSKYIASKIVKEMLSKGLTVNKSNVNVLGLTFK
ENCPDLRNTKVIDIIRELEEYGITVNVHDAKADKEEAKKFYNIELKEVNELPETDLVLFAVSHNEYIQNKEEFLKKLSNN
GIVFDIKGIINKEDTSNNQTIWSL
>Mature_424_residues
MNRKIAVVGLGYVGLPVAAAFGKLHEVIGFDINESRIQELKDGYDRTNEVKSEDLAATKINFTSNKSDLAKADFIIVAVP
TPINKNNQPDLTPLIKASETVGSAMEKGTIVVYESTVYPGATREDCVPVLEQFSGLKCGEDFFVGYSPERINPGDKVHTF
ETITKVVSGQTPEVLEIVADVYGSVVKAGVHKAPTIEVAEAAKVIENTQRDINIALMNELALIFDKAGINTKDVLEAAGT
KWNFLKFTPGLVGGHCIGVDPYYLTHKAQELGHHPEVILSGRRINDGMSKYIASKIVKEMLSKGLTVNKSNVNVLGLTFK
ENCPDLRNTKVIDIIRELEEYGITVNVHDAKADKEEAKKFYNIELKEVNELPETDLVLFAVSHNEYIQNKEEFLKKLSNN
GIVFDIKGIINKEDTSNNQTIWSL

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=406, Percent_Identity=29.3103448275862, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788340, Length=426, Percent_Identity=24.6478873239437, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560350, Length=380, Percent_Identity=26.0526315789474, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46768; Mature: 46768

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKIAVVGLGYVGLPVAAAFGKLHEVIGFDINESRIQELKDGYDRTNEVKSEDLAATKI
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHCCCCCCEEEEE
NFTSNKSDLAKADFIIVAVPTPINKNNQPDLTPLIKASETVGSAMEKGTIVVYESTVYPG
EECCCCCHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCC
ATREDCVPVLEQFSGLKCGEDFFVGYSPERINPGDKVHTFETITKVVSGQTPEVLEIVAD
CCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
VYGSVVKAGVHKAPTIEVAEAAKVIENTQRDINIALMNELALIFDKAGINTKDVLEAAGT
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHCCC
KWNFLKFTPGLVGGHCIGVDPYYLTHKAQELGHHPEVILSGRRINDGMSKYIASKIVKEM
CCEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHH
LSKGLTVNKSNVNVLGLTFKENCPDLRNTKVIDIIRELEEYGITVNVHDAKADKEEAKKF
HHCCCEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
YNIELKEVNELPETDLVLFAVSHNEYIQNKEEFLKKLSNNGIVFDIKGIINKEDTSNNQT
HCCCHHHHCCCCCCCEEEEEECCCHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCE
IWSL
EECC
>Mature Secondary Structure
MNRKIAVVGLGYVGLPVAAAFGKLHEVIGFDINESRIQELKDGYDRTNEVKSEDLAATKI
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHCCCCCCEEEEE
NFTSNKSDLAKADFIIVAVPTPINKNNQPDLTPLIKASETVGSAMEKGTIVVYESTVYPG
EECCCCCHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCC
ATREDCVPVLEQFSGLKCGEDFFVGYSPERINPGDKVHTFETITKVVSGQTPEVLEIVAD
CCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
VYGSVVKAGVHKAPTIEVAEAAKVIENTQRDINIALMNELALIFDKAGINTKDVLEAAGT
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHCCC
KWNFLKFTPGLVGGHCIGVDPYYLTHKAQELGHHPEVILSGRRINDGMSKYIASKIVKEM
CCEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHH
LSKGLTVNKSNVNVLGLTFKENCPDLRNTKVIDIIRELEEYGITVNVHDAKADKEEAKKF
HHCCCEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
YNIELKEVNELPETDLVLFAVSHNEYIQNKEEFLKKLSNNGIVFDIKGIINKEDTSNNQT
HCCCHHHHCCCCCCCEEEEEECCCHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCE
IWSL
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]