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The map label for this gene is fabI [H]

Identifier: 222150875

GI number: 222150875

Start: 668917

End: 669690

Strand: Direct

Name: fabI [H]

Synonym: MCCL_0625

Alternate gene names: 222150875

Gene position: 668917-669690 (Clockwise)

Preceding gene: 222150874

Following gene: 222150878

Centisome position: 31.82

GC content: 40.7

Gene sequence:

>774_bases
ATGTTAAATTTTCAGGATAAAGTGTATGTCATTATGGGTGTAGCCAATAAACGCAGTATCGGATATGGTGTTGCACAAGT
TTTAGATGAAGTTGGTGCTAAGCTGATATTTACATATCGTAAAGAACGCAGCATGAAAGAACTGGAACGTCTGCTTCCTA
ATTTAAAGCATAATCATGATGCACTGATGATTCAATGTGATGTGCAAGAAGATGCGGATGTGGTAAGTGCGTTTGAACAG
ATTAAATCTGAAGTAGGGCATATCGATGGAGTATTCCATTCGATCGCTTTTGCTAATATGGAAGATCTGCGCGGCCGCTT
TATTGATACGACCCGTGAAGGCTTCTTACTCGCACAGGATATCAGCTCATATTCATTAACAATTGTAGCGAGAGAAGCCA
GTAAGTTAATGTCTGAAGGCGGGTCTATCGTGACAACGACATATCTTGGAGGAGAATTCGCAATTCCGAACTATAACGTT
ATGGGCGTGGCAAAAGCAAGCCTTGAAGCCAATGTTAAATATTTAGCGTTAGACTTAGGTCAAGATGGTATCCGTGTGAA
CGCTGTATCACCAGGACCGATTCGTACGTTAAGCGCAAAAGGCATCGGAGACTTTAATACGATATTAAAACGTATCGAAA
GTGAAGCACCTTTAAAGCGCAACGTAGATGCCTTAGAAGTTGGTAAGACAGCACTGTATCTGTTAAGTGACTTATCAAGT
GGTGTTACCGGAGAAAATATTCATGTGGATAGCGGTTATCATATCGTAAGATAA

Upstream 100 bases:

>100_bases
TATTTCTTAAAAATATATGATTTTTTTACTGAAATTCAAATGAACTTCTGATATATTTAGTAGTAGGTACTAGAACTTAC
TTTTATAAAAGGAGACTTAT

Downstream 100 bases:

>100_bases
AGTTGGATCATAAAACAAGCGGCTAGGACATGTGTCCTAGCCGTTTAGGTTATTCACCGAGTTTAAATGTTTCCAGTACA
TTCCATGTTCCGTTTTCTAG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLNFQDKVYVIMGVANKRSIGYGVAQVLDEVGAKLIFTYRKERSMKELERLLPNLKHNHDALMIQCDVQEDADVVSAFEQ
IKSEVGHIDGVFHSIAFANMEDLRGRFIDTTREGFLLAQDISSYSLTIVAREASKLMSEGGSIVTTTYLGGEFAIPNYNV
MGVAKASLEANVKYLALDLGQDGIRVNAVSPGPIRTLSAKGIGDFNTILKRIESEAPLKRNVDALEVGKTALYLLSDLSS
GVTGENIHVDSGYHIVR

Sequences:

>Translated_257_residues
MLNFQDKVYVIMGVANKRSIGYGVAQVLDEVGAKLIFTYRKERSMKELERLLPNLKHNHDALMIQCDVQEDADVVSAFEQ
IKSEVGHIDGVFHSIAFANMEDLRGRFIDTTREGFLLAQDISSYSLTIVAREASKLMSEGGSIVTTTYLGGEFAIPNYNV
MGVAKASLEANVKYLALDLGQDGIRVNAVSPGPIRTLSAKGIGDFNTILKRIESEAPLKRNVDALEVGKTALYLLSDLSS
GVTGENIHVDSGYHIVR
>Mature_257_residues
MLNFQDKVYVIMGVANKRSIGYGVAQVLDEVGAKLIFTYRKERSMKELERLLPNLKHNHDALMIQCDVQEDADVVSAFEQ
IKSEVGHIDGVFHSIAFANMEDLRGRFIDTTREGFLLAQDISSYSLTIVAREASKLMSEGGSIVTTTYLGGEFAIPNYNV
MGVAKASLEANVKYLALDLGQDGIRVNAVSPGPIRTLSAKGIGDFNTILKRIESEAPLKRNVDALEVGKTALYLLSDLSS
GVTGENIHVDSGYHIVR

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=243, Percent_Identity=26.7489711934156, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI40254992, Length=253, Percent_Identity=25.6916996047431, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787545, Length=250, Percent_Identity=45.6, Blast_Score=230, Evalue=7e-62,
Organism=Escherichia coli, GI87082100, Length=245, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787335, Length=260, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367175, Length=251, Percent_Identity=26.2948207171315, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789378, Length=147, Percent_Identity=31.2925170068027, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788459, Length=240, Percent_Identity=25.8333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=26.865671641791, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=257, Percent_Identity=25.6809338521401, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=269, Percent_Identity=27.5092936802974, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397609, Length=258, Percent_Identity=27.1317829457364, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571526, Length=263, Percent_Identity=30.0380228136882, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355319, Length=258, Percent_Identity=26.3565891472868, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=25.7692307692308, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357041, Length=262, Percent_Identity=27.0992366412214, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24644337, Length=120, Percent_Identity=36.6666666666667, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28156; Mature: 28156

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFQDKVYVIMGVANKRSIGYGVAQVLDEVGAKLIFTYRKERSMKELERLLPNLKHNHD
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHCCCCCCC
ALMIQCDVQEDADVVSAFEQIKSEVGHIDGVFHSIAFANMEDLRGRFIDTTREGFLLAQD
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEECCCCCCEEEEEC
ISSYSLTIVAREASKLMSEGGSIVTTTYLGGEFAIPNYNVMGVAKASLEANVKYLALDLG
CCCEEEEEEEHHHHHHHHCCCCEEEEEEECCEEECCCCCEEEEEECHHHCCEEEEEEEEC
QDGIRVNAVSPGPIRTLSAKGIGDFNTILKRIESEAPLKRNVDALEVGKTALYLLSDLSS
CCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHC
GVTGENIHVDSGYHIVR
CCCCCEEEECCCCEECC
>Mature Secondary Structure
MLNFQDKVYVIMGVANKRSIGYGVAQVLDEVGAKLIFTYRKERSMKELERLLPNLKHNHD
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHCCCCCCC
ALMIQCDVQEDADVVSAFEQIKSEVGHIDGVFHSIAFANMEDLRGRFIDTTREGFLLAQD
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEECCCCCCEEEEEC
ISSYSLTIVAREASKLMSEGGSIVTTTYLGGEFAIPNYNVMGVAKASLEANVKYLALDLG
CCCEEEEEEEHHHHHHHHCCCCEEEEEEECCEEECCCCCEEEEEECHHHCCEEEEEEEEC
QDGIRVNAVSPGPIRTLSAKGIGDFNTILKRIESEAPLKRNVDALEVGKTALYLLSDLSS
CCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHC
GVTGENIHVDSGYHIVR
CCCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]