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The map label for this gene is yugI [H]

Identifier: 222150831

GI number: 222150831

Start: 616105

End: 616473

Strand: Direct

Name: yugI [H]

Synonym: MCCL_0581

Alternate gene names: 222150831

Gene position: 616105-616473 (Clockwise)

Preceding gene: 222150830

Following gene: 222150832

Centisome position: 29.31

GC content: 36.31

Gene sequence:

>369_bases
ATGAAGAAAACTTATAAAAAAGGACAGTTTATAAAAGTAAAGGTTACTGGTATTCAGCCGTATGGAGCATTCGTTGAAAC
TGAGGACCACACTGAAGGCCTGATTCATATTTCAGAAGTGATGAACGATTATGTCCATAATTTGAATTTATTGCTTTCTG
TGGGACAGATTGTGAAGGCCAAAGTGTTGAATGTTTCAGAAGATGGCAAACTGAACCTAAGTTTAAAAGAAAATGACTAT
TTTAAGAAACAAGAAAGAAAGAAAACGAAACGTTCTGTGCTAGAACAACTCAATGAGCCTGAATCTATTGGGTTTAAGAC
GCTTCAAGAACGTTTGCCTGGGTGGATTGAACGCGGATTAAAGCTATAA

Upstream 100 bases:

>100_bases
AATATATGCATTAGATCAATCATGTGGAGTATAGACTTCACATGATTTTTTTTAATGATTTAATTATGATAAAATAAGCT
AAAGAAAATGAGGGATGATG

Downstream 100 bases:

>100_bases
TTTTTATGCACGAATATGTATAAATAATTTTTGTAAAATTTCGAGCATTTAGGGTTGAACTATTGTAAGAAATTGTTCAT
TATATGTATGTAAGGGTTTT

Product: general stress protein 13

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: GSP13 [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MKKTYKKGQFIKVKVTGIQPYGAFVETEDHTEGLIHISEVMNDYVHNLNLLLSVGQIVKAKVLNVSEDGKLNLSLKENDY
FKKQERKKTKRSVLEQLNEPESIGFKTLQERLPGWIERGLKL

Sequences:

>Translated_122_residues
MKKTYKKGQFIKVKVTGIQPYGAFVETEDHTEGLIHISEVMNDYVHNLNLLLSVGQIVKAKVLNVSEDGKLNLSLKENDY
FKKQERKKTKRSVLEQLNEPESIGFKTLQERLPGWIERGLKL
>Mature_122_residues
MKKTYKKGQFIKVKVTGIQPYGAFVETEDHTEGLIHISEVMNDYVHNLNLLLSVGQIVKAKVLNVSEDGKLNLSLKENDY
FKKQERKKTKRSVLEQLNEPESIGFKTLQERLPGWIERGLKL

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

None

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR000110
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 14058; Mature: 14058

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50126 S1 ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTYKKGQFIKVKVTGIQPYGAFVETEDHTEGLIHISEVMNDYVHNLNLLLSVGQIVKA
CCCCCCCCCEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVLNVSEDGKLNLSLKENDYFKKQERKKTKRSVLEQLNEPESIGFKTLQERLPGWIERGL
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHCC
KL
CC
>Mature Secondary Structure
MKKTYKKGQFIKVKVTGIQPYGAFVETEDHTEGLIHISEVMNDYVHNLNLLLSVGQIVKA
CCCCCCCCCEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVLNVSEDGKLNLSLKENDYFKKQERKKTKRSVLEQLNEPESIGFKTLQERLPGWIERGL
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHCC
KL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 9298659 [H]