BacMap

Definition	Macrococcus caseolyticus JCSC5402, complete genome.
Accession	NC_011999
Length	2,102,324

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The map label for this gene is yqhE [C]

Identifier: 222150710

GI number: 222150710

Start: 486207

End: 487043

Strand: Direct

Name: yqhE [C]

Synonym: MCCL_0460

Alternate gene names: 222150710

Gene position: 486207-487043 (Clockwise)

Preceding gene: 222150708

Following gene: 222150711

Centisome position: 23.13

GC content: 37.04

Gene sequence:

>837_bases
ATGATGAACCATATTCAGCTAAACAATGGTGTAACTATGCCACAAATTGGACTTGGTGTCTACAAGATTTCGGATGAAAC
AATGTCAGAGGTCGTCAATTCCGCACTAGATGCGGGATACCGTGCCTTCGATACGGCGCACTTCTATTTTAATGAAGCCT
CGCTTGGTAAAGCATTGAAAGAAAGCAATATACCACGAGAAGAACTGTTTATTACGACGAAACTGTGGAATGATCATCAG
GGATATGAATCAACGAAGAAAGCATTCAATGATTCACTTCAGAAATTAGATATGGATTATGTAGATCTTTATTTGATTCA
CTGGCCATGTCCGGAAGACGGATTATTCGTTGAAAGTTACAAAGCGATGGAAGAACTGTATCATGAAGGTAAGATCAAAG
CATTAGGTGTTGCTAACTTTAAAGAACATCATCTGGATAAGCTGTTACAGGAAACAACTGTAGTGCCTGCAGTAAATCAA
ATCGAGTATCATCCGATCTTTAATCAAGACAGTTTGCAGCAATACTGTAAAGATAAAGGCATTGCAGTTACTGCATGGAG
TCCGTTAATGCGTGGTGGAGCACTGTTTGAAGATGAAACATTGAAACGCATCGCAGAAAAATATAACAAAACAGTAGCGC
AGATCATTATCAGATGGCATATCGACAGTGGACGAATCGTCATTCCTAAGTCATCAAACATAGAACGTATTAAAGAGAAT
ATTGATGTATGTCATTTTGAGTTAATGGAACAAGATATAGAAGCAATTAATAATTTAAATAGAAATGAACGTCAATTTAA
AGATCCGGATAAGATTAAAATTGGGGATATGAAGTAA

Upstream 100 bases:

>100_bases
TATCATTATTATACTAAATATTTGAAGCGTCTAAAAATATAATGATATTCATTTTGTAAGAAAGCTTGTTTAGTGTATGA
TTTAAATAAAGGAGATGATA

Downstream 100 bases:

>100_bases
ATATTGAATAAACGATGAGATGGTGATGTTCAATGGCACCATCTCTTTTTCATGAGTTCGTCACAATTAATAGTTTTTAA
CATATATATTAAATGTATGT

Product: aldo/keto reductase family protein

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MMNHIQLNNGVTMPQIGLGVYKISDETMSEVVNSALDAGYRAFDTAHFYFNEASLGKALKESNIPREELFITTKLWNDHQ
GYESTKKAFNDSLQKLDMDYVDLYLIHWPCPEDGLFVESYKAMEELYHEGKIKALGVANFKEHHLDKLLQETTVVPAVNQ
IEYHPIFNQDSLQQYCKDKGIAVTAWSPLMRGGALFEDETLKRIAEKYNKTVAQIIIRWHIDSGRIVIPKSSNIERIKEN
IDVCHFELMEQDIEAINNLNRNERQFKDPDKIKIGDMK

Sequences:

>Translated_278_residues
MMNHIQLNNGVTMPQIGLGVYKISDETMSEVVNSALDAGYRAFDTAHFYFNEASLGKALKESNIPREELFITTKLWNDHQ
GYESTKKAFNDSLQKLDMDYVDLYLIHWPCPEDGLFVESYKAMEELYHEGKIKALGVANFKEHHLDKLLQETTVVPAVNQ
IEYHPIFNQDSLQQYCKDKGIAVTAWSPLMRGGALFEDETLKRIAEKYNKTVAQIIIRWHIDSGRIVIPKSSNIERIKEN
IDVCHFELMEQDIEAINNLNRNERQFKDPDKIKIGDMK
>Mature_278_residues
MMNHIQLNNGVTMPQIGLGVYKISDETMSEVVNSALDAGYRAFDTAHFYFNEASLGKALKESNIPREELFITTKLWNDHQ
GYESTKKAFNDSLQKLDMDYVDLYLIHWPCPEDGLFVESYKAMEELYHEGKIKALGVANFKEHHLDKLLQETTVVPAVNQ
IEYHPIFNQDSLQQYCKDKGIAVTAWSPLMRGGALFEDETLKRIAEKYNKTVAQIIIRWHIDSGRIVIPKSSNIERIKEN
IDVCHFELMEQDIEAINNLNRNERQFKDPDKIKIGDMK

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=297, Percent_Identity=40.0673400673401, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=36.6438356164384, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=36.6438356164384, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI4502049, Length=297, Percent_Identity=41.4141414141414, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI93277124, Length=298, Percent_Identity=37.248322147651, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=39.7058823529412, Blast_Score=188, Evalue=4e-48,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=36.2007168458781, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI5174695, Length=300, Percent_Identity=34.6666666666667, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI310109920, Length=297, Percent_Identity=34.6801346801347, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI5453543, Length=297, Percent_Identity=34.3434343434343, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI45446745, Length=297, Percent_Identity=35.016835016835, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI4503285, Length=297, Percent_Identity=35.016835016835, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI24497583, Length=299, Percent_Identity=34.1137123745819, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=33.2214765100671, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI300116273, Length=279, Percent_Identity=34.7670250896057, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI300116271, Length=280, Percent_Identity=33.2142857142857, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=35.7142857142857, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI310109926, Length=113, Percent_Identity=38.0530973451327, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=38.9380530973451, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=48.134328358209, Blast_Score=266, Evalue=8e-73,
Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=37.890625, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788081, Length=273, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788070, Length=307, Percent_Identity=25.7328990228013, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081735, Length=286, Percent_Identity=23.7762237762238, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994888, Length=274, Percent_Identity=27.007299270073, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71998625, Length=274, Percent_Identity=39.051094890511, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17537075, Length=310, Percent_Identity=36.7741935483871, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17537077, Length=296, Percent_Identity=36.1486486486487, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17564128, Length=303, Percent_Identity=36.6336633663366, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=35.5311355311355, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=37.5, Blast_Score=183, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=37.6383763837638, Blast_Score=173, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=34.0659340659341, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537079, Length=293, Percent_Identity=31.3993174061433, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17566692, Length=298, Percent_Identity=35.9060402684564, Blast_Score=166, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17550248, Length=292, Percent_Identity=33.5616438356164, Blast_Score=160, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17562292, Length=278, Percent_Identity=30.5755395683453, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320576, Length=286, Percent_Identity=40.5594405594406, Blast_Score=212, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324694, Length=286, Percent_Identity=39.8601398601399, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6322556, Length=262, Percent_Identity=36.6412213740458, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321896, Length=316, Percent_Identity=32.2784810126582, Blast_Score=169, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319625, Length=293, Percent_Identity=35.4948805460751, Blast_Score=168, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6320079, Length=278, Percent_Identity=32.7338129496403, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24662789, Length=292, Percent_Identity=40.4109589041096, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=39.3728222996516, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=35.1724137931034, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=36.7892976588629, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45553081, Length=292, Percent_Identity=35.6164383561644, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=34.4827586206897, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129731, Length=285, Percent_Identity=34.7368421052632, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=35.3951890034364, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24662781, Length=292, Percent_Identity=38.3561643835616, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=35.2713178294574, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24662785, Length=287, Percent_Identity=38.3275261324042, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32101; Mature: 32101

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNHIQLNNGVTMPQIGLGVYKISDETMSEVVNSALDAGYRAFDTAHFYFNEASLGKALK
CCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHEEECHHHHHHHHH
ESNIPREELFITTKLWNDHQGYESTKKAFNDSLQKLDMDYVDLYLIHWPCPEDGLFVESY
HCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEHHHH
KAMEELYHEGKIKALGVANFKEHHLDKLLQETTVVPAVNQIEYHPIFNQDSLQQYCKDKG
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCC
IAVTAWSPLMRGGALFEDETLKRIAEKYNKTVAQIIIRWHIDSGRIVIPKSSNIERIKEN
EEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEECCCCHHHHHHH
IDVCHFELMEQDIEAINNLNRNERQFKDPDKIKIGDMK
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEECCCCC
>Mature Secondary Structure
MMNHIQLNNGVTMPQIGLGVYKISDETMSEVVNSALDAGYRAFDTAHFYFNEASLGKALK
CCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHEEECHHHHHHHHH
ESNIPREELFITTKLWNDHQGYESTKKAFNDSLQKLDMDYVDLYLIHWPCPEDGLFVESY
HCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEHHHH
KAMEELYHEGKIKALGVANFKEHHLDKLLQETTVVPAVNQIEYHPIFNQDSLQQYCKDKG
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCC
IAVTAWSPLMRGGALFEDETLKRIAEKYNKTVAQIIIRWHIDSGRIVIPKSSNIERIKEN
EEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEECCCCHHHHHHH
IDVCHFELMEQDIEAINNLNRNERQFKDPDKIKIGDMK
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA