Click here to switch to the map view.

The map label for this gene is rluF [H]

Identifier: 222150708

GI number: 222150708

Start: 484521

End: 485231

Strand: Direct

Name: rluF [H]

Synonym: MCCL_0458

Alternate gene names: 222150708

Gene position: 484521-485231 (Clockwise)

Preceding gene: 222150707

Following gene: 222150710

Centisome position: 23.05

GC content: 36.43

Gene sequence:

>711_bases
ATGAGAATTAATAAGTTTTTATCAGAAGCAGGCATTTGCTCACGTCGTGGAGCTGATAAGTGGATTGAGGCAGGACGCAT
AAAGATTAATGGTGTTAAAGCTGAACTTGGCAGTAAGGTGACAGAAGGTGATAGTGTGGAAGTCGATAACAAAGTTGTGA
CATTGAACACGGATAAAGGATACGTATATATTAAACTGAATAAACCTCGTGGTATTACATCGACAACTGAGCGCCATATT
AAAGGGAATATCGTTGATTTTGTGGGACATAGTGAACGTATATTTCATATCGGTAGATTAGATAAAGATTCAGAAGGCTT
AATCTTACTCACGAACGATGGTGATATCGTAAACGAAATATTACGTAAGGAAGGCCGACATGAAAAAGAATATATCGTAA
CTGTTGATAAACCGATAACTGATGATTTTGTGAAACAGATGTCTGAAGGTGTTGCAATATTGGATACTGTGACATTACCT
GCAGAAGTTGAGAAGTTAAATACAAAGACATTCAAAATTATATTAACGCAAGGATTGAATCGACAAATTAGGCGTATGAC
CGAAGCATTAGGATATGAAGTAAAGCAATTACGTCGCATTCGCGTGATGAATATATTGGTGGATGATTTAAAACTTGGGG
AATGGAAAGATTTAACTGAAGCTGAAAAGGATGAACTGTTTTCGATGTTAAAGTATGTGCCAAAGCGTTAA

Upstream 100 bases:

>100_bases
CATTTGTATATGATCATTATTATATTCGTTCAAAATATTATTGAAGCGTGTGAGGCAGGTAATGCCTCAGACGCTTTTAC
TTTGAAAGGTTGCTGGTTGT

Downstream 100 bases:

>100_bases
ACATATGTAAATCAGAAAAAAAGCGTCAAAACGATAAAGAAAATATCGTTCTGGCGCTTTTATGCATTAATCACTGAACA
TATCAAATAGTCCACCGATA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRINKFLSEAGICSRRGADKWIEAGRIKINGVKAELGSKVTEGDSVEVDNKVVTLNTDKGYVYIKLNKPRGITSTTERHI
KGNIVDFVGHSERIFHIGRLDKDSEGLILLTNDGDIVNEILRKEGRHEKEYIVTVDKPITDDFVKQMSEGVAILDTVTLP
AEVEKLNTKTFKIILTQGLNRQIRRMTEALGYEVKQLRRIRVMNILVDDLKLGEWKDLTEAEKDELFSMLKYVPKR

Sequences:

>Translated_236_residues
MRINKFLSEAGICSRRGADKWIEAGRIKINGVKAELGSKVTEGDSVEVDNKVVTLNTDKGYVYIKLNKPRGITSTTERHI
KGNIVDFVGHSERIFHIGRLDKDSEGLILLTNDGDIVNEILRKEGRHEKEYIVTVDKPITDDFVKQMSEGVAILDTVTLP
AEVEKLNTKTFKIILTQGLNRQIRRMTEALGYEVKQLRRIRVMNILVDDLKLGEWKDLTEAEKDELFSMLKYVPKR
>Mature_236_residues
MRINKFLSEAGICSRRGADKWIEAGRIKINGVKAELGSKVTEGDSVEVDNKVVTLNTDKGYVYIKLNKPRGITSTTERHI
KGNIVDFVGHSERIFHIGRLDKDSEGLILLTNDGDIVNEILRKEGRHEKEYIVTVDKPITDDFVKQMSEGVAILDTVTLP
AEVEKLNTKTFKIILTQGLNRQIRRMTEALGYEVKQLRRIRVMNILVDDLKLGEWKDLTEAEKDELFSMLKYVPKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=54.978354978355, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788510, Length=228, Percent_Identity=30.7017543859649, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=28.5140562248996, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=33.5616438356164, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26912; Mature: 26912

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINKFLSEAGICSRRGADKWIEAGRIKINGVKAELGSKVTEGDSVEVDNKVVTLNTDKG
CCHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEHHHHCCCCCCCCCEEECCEEEEEECCCC
YVYIKLNKPRGITSTTERHIKGNIVDFVGHSERIFHIGRLDKDSEGLILLTNDGDIVNEI
EEEEEECCCCCCCCCHHHHHCCCEEEECCCCCEEEEEECCCCCCCCEEEEECCCHHHHHH
LRKEGRHEKEYIVTVDKPITDDFVKQMSEGVAILDTVTLPAEVEKLNTKTFKIILTQGLN
HHHCCCCCCEEEEEECCCCCHHHHHHHHCCEEEEEEECCCHHHHHCCCCEEEEEEECCCC
RQIRRMTEALGYEVKQLRRIRVMNILVDDLKLGEWKDLTEAEKDELFSMLKYVPKR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRINKFLSEAGICSRRGADKWIEAGRIKINGVKAELGSKVTEGDSVEVDNKVVTLNTDKG
CCHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEHHHHCCCCCCCCCEEECCEEEEEECCCC
YVYIKLNKPRGITSTTERHIKGNIVDFVGHSERIFHIGRLDKDSEGLILLTNDGDIVNEI
EEEEEECCCCCCCCCHHHHHCCCEEEECCCCCEEEEEECCCCCCCCEEEEECCCHHHHHH
LRKEGRHEKEYIVTVDKPITDDFVKQMSEGVAILDTVTLPAEVEKLNTKTFKIILTQGLN
HHHCCCCCCEEEEEECCCCCHHHHHHHHCCEEEEEEECCCHHHHHCCCCEEEEEEECCCC
RQIRRMTEALGYEVKQLRRIRVMNILVDDLKLGEWKDLTEAEKDELFSMLKYVPKR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]