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The map label for this gene is bioB

Identifier: 222150626

GI number: 222150626

Start: 401082

End: 402074

Strand: Direct

Name: bioB

Synonym: MCCL_0376

Alternate gene names: 222150626

Gene position: 401082-402074 (Clockwise)

Preceding gene: 222150625

Following gene: 222150628

Centisome position: 19.08

GC content: 38.57

Gene sequence:

>993_bases
ATGTTAGCACAACGTATTATTAATGGAGAAAATATTTCAAAAGAAGAAGCATTATCATTATTTGTTGATAATAATATAGA
TACATATGACTTACTACATGAAGCATATCAAGTGCGTAAATACTATTATGGTCGTAAGGTGAAACTGAATATGATTCTTA
ATGCGAAGAGTGGAATTTGTCCAGAAGACTGTGGTTATTGCGGACAATCGAAACTGATGAAGAATAAAGACAAATATTTA
CTGGTTGATAGTGAACAGATTAAATCAGGTGCAGATTTCTGTGCGACACATGACATCGGCACTTATTGTATCGTTATGAG
TGGGCGAGGGCCAAGTGATAAAGAGGTTGAGCATATTGCAGATACTGTTGAAGCAATTAAACACGAACATCCGCAGCTTA
AGATATGTGCTTGTCTCGGTCTGACGAATGACGAACAAGCGAAGAAGCTGAAATTGGCTGGCGTCGATCGTTATAATCAC
AATATTAATACGAGTGAAAATTATCATGACGAAGTCGTAACGACACATACATATCAAGACCGTGTCGATACCATTGAAAT
CATGAAAGCAAATAACATTTCACCTTGCTCAGGTGTGATATGTGGGATGGGGGAAACAGATGAGGATATTATTGATATGG
CATTAGCGCTTCGAGCAATAGACGCAGATAGTATCCCGGTAAACTTCCTTCACCCTGTGAAGGGGACGAAGTTCGGTAAT
GAAGATAACTTAACACCAGAACGCTGTTTAAGAATATTGTCGATGTTCAGATTGATTAATCCGTCGAAAGAAATCCGTAT
TGCCGGGGGTAGAGAAGTCAACTTAAGAACATTACAACCCCTGGCGATGCAGGCAGCAAATTCCATCTTTGTGGGGGATT
ATCTGATTACCGATGGACAGCCGAATGAACTCGATTATAAGATGCTGGAAGACCTAGGTTTTGAGATTGATATAAATGAA
TCTGAAATAAATAAAGCAGCACTCAAAATTTGA

Upstream 100 bases:

>100_bases
ATCGCCCGTTTATTTTTTTGGATGATTGTTAACTTATATTCAAAATTAGTTTACAATATAATAATGCAGTTATATTATAA
AAGTATACAGGGGGGATTTT

Downstream 100 bases:

>100_bases
GTGCTGCTTTATTCACTACCATTATGAATCAACGCAAAAGGATAGACTTTATCAGCGCTTTCTAGCAGCTGTGATGCAAT
AACGGTCATCGTCCATCTAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MLAQRIINGENISKEEALSLFVDNNIDTYDLLHEAYQVRKYYYGRKVKLNMILNAKSGICPEDCGYCGQSKLMKNKDKYL
LVDSEQIKSGADFCATHDIGTYCIVMSGRGPSDKEVEHIADTVEAIKHEHPQLKICACLGLTNDEQAKKLKLAGVDRYNH
NINTSENYHDEVVTTHTYQDRVDTIEIMKANNISPCSGVICGMGETDEDIIDMALALRAIDADSIPVNFLHPVKGTKFGN
EDNLTPERCLRILSMFRLINPSKEIRIAGGREVNLRTLQPLAMQAANSIFVGDYLITDGQPNELDYKMLEDLGFEIDINE
SEINKAALKI

Sequences:

>Translated_330_residues
MLAQRIINGENISKEEALSLFVDNNIDTYDLLHEAYQVRKYYYGRKVKLNMILNAKSGICPEDCGYCGQSKLMKNKDKYL
LVDSEQIKSGADFCATHDIGTYCIVMSGRGPSDKEVEHIADTVEAIKHEHPQLKICACLGLTNDEQAKKLKLAGVDRYNH
NINTSENYHDEVVTTHTYQDRVDTIEIMKANNISPCSGVICGMGETDEDIIDMALALRAIDADSIPVNFLHPVKGTKFGN
EDNLTPERCLRILSMFRLINPSKEIRIAGGREVNLRTLQPLAMQAANSIFVGDYLITDGQPNELDYKMLEDLGFEIDINE
SEINKAALKI
>Mature_330_residues
MLAQRIINGENISKEEALSLFVDNNIDTYDLLHEAYQVRKYYYGRKVKLNMILNAKSGICPEDCGYCGQSKLMKNKDKYL
LVDSEQIKSGADFCATHDIGTYCIVMSGRGPSDKEVEHIADTVEAIKHEHPQLKICACLGLTNDEQAKKLKLAGVDRYNH
NINTSENYHDEVVTTHTYQDRVDTIEIMKANNISPCSGVICGMGETDEDIIDMALALRAIDADSIPVNFLHPVKGTKFGN
EDNLTPERCLRILSMFRLINPSKEIRIAGGREVNLRTLQPLAMQAANSIFVGDYLITDGQPNELDYKMLEDLGFEIDINE
SEINKAALKI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=291, Percent_Identity=34.3642611683849, Blast_Score=175, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321725, Length=288, Percent_Identity=33.6805555555556, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_MACCJ (B9EA22)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559779.1
- GeneID:   7391074
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0376
- OMA:   MGMETCM
- ProtClustDB:   PRK06256
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36984; Mature: 36984

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQRIINGENISKEEALSLFVDNNIDTYDLLHEAYQVRKYYYGRKVKLNMILNAKSGIC
CCHHHCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
PEDCGYCGQSKLMKNKDKYLLVDSEQIKSGADFCATHDIGTYCIVMSGRGPSDKEVEHIA
HHHCCCCCCHHHHCCCCCEEEEEHHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHHHH
DTVEAIKHEHPQLKICACLGLTNDEQAKKLKLAGVDRYNHNINTSENYHDEVVTTHTYQD
HHHHHHHCCCCCEEEEEEECCCCCHHHHEEEEECCHHCCCCCCCCCCCCCCEEEECCHHC
RVDTIEIMKANNISPCSGVICGMGETDEDIIDMALALRAIDADSIPVNFLHPVKGTKFGN
CCCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
EDNLTPERCLRILSMFRLINPSKEIRIAGGREVNLRTLQPLAMQAANSIFVGDYLITDGQ
CCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHCCCEEEEEEEEECCC
PNELDYKMLEDLGFEIDINESEINKAALKI
CCCCHHHHHHHCCCEEECCHHHHHHHHHCC
>Mature Secondary Structure
MLAQRIINGENISKEEALSLFVDNNIDTYDLLHEAYQVRKYYYGRKVKLNMILNAKSGIC
CCHHHCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
PEDCGYCGQSKLMKNKDKYLLVDSEQIKSGADFCATHDIGTYCIVMSGRGPSDKEVEHIA
HHHCCCCCCHHHHCCCCCEEEEEHHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHHHH
DTVEAIKHEHPQLKICACLGLTNDEQAKKLKLAGVDRYNHNINTSENYHDEVVTTHTYQD
HHHHHHHCCCCCEEEEEEECCCCCHHHHEEEEECCHHCCCCCCCCCCCCCCEEEECCHHC
RVDTIEIMKANNISPCSGVICGMGETDEDIIDMALALRAIDADSIPVNFLHPVKGTKFGN
CCCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
EDNLTPERCLRILSMFRLINPSKEIRIAGGREVNLRTLQPLAMQAANSIFVGDYLITDGQ
CCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHCCCEEEEEEEEECCC
PNELDYKMLEDLGFEIDINESEINKAALKI
CCCCHHHHHHHCCCEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA