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The map label for this gene is sacP [H]

Identifier: 222150625

GI number: 222150625

Start: 399542

End: 400960

Strand: Direct

Name: sacP [H]

Synonym: MCCL_0375

Alternate gene names: 222150625

Gene position: 399542-400960 (Clockwise)

Preceding gene: 222150624

Following gene: 222150626

Centisome position: 19.0

GC content: 39.25

Gene sequence:

>1419_bases
ATGGCTAAAACAGACAAACAGATTGCACAGGAAGTACTCGACGCAGTAGGAGGACGTGAAAATATTGCTTCGATTGCCCA
TTGTGCAACGCGATTACGCATTATGGTGAACGACAAAGAAAAAGTGAATCAAGAACAAGTAGAGGATATCGAAAAAGTAA
AAGGTGCATTCTACAACTCTGGTCAGTATCAAATTATATTTGGAACTGGAACAGTTAATAAGATTTACGATGCGATTACG
AGTTTAGGTATCGTTGGAAAATCAACAGATGAAGTGAAAACAGAAGGAAAAAAACAAGGCAATGCATTCCAGCGTGCCAT
ACGCGTTTTCGGTGACGTATTCGTCCCTATTATTCCAGTACTTGTTGCTACCGGCTTATTTATGGGGTTACGTGGTGTAC
TGATGAATGAGCAGATTCTTGAATGGATGGGTATGACACCAAAGGACATCTCGCCTAACTTCATATTATTTACGCAAGTA
CTAACAGACACTGCGTTTGCATTCTTACCAGCATTAGTTGCATGGAGTGCATTTAAAGTCTTTGGTGGTAGTCCGGTATT
AGGTATTGTTCTTGGTTTGATGTTAGTCAATCCAGCACTTCCTAACGCATATGCAGTAGGTGATGGATCAGCTGAAGCAT
TAAAATTCTTCGGTTTTATTCCGGTGGTGGGCTACCAAGGTTCTGTTTTACCTGCCTTCTTTGTTGGGCTAATTGGCGCA
AAGTTTGAACAGTTATTAAGAAAACGTATACCGGATGCAATAGACCTCATTGTTACACCTTTCGTTACGTTACTCGTGAT
GATTACACTTGGACTCTTTGTGATTGGACCTGTATTCCACGGTCTAGAAACGATTATCTTGACAGGCACAAAATGGGTGC
TTGATCTACCATATGGTATAGCAGGTCTAATTATCGGTTTCTTCCAGCAGATCATTGTAGTAACAGGTGTACACCACATC
TTTAACTTTATGGAAATTCAGTTACTGGAAGAATTCAAATATAATGAATTCAATCCAATTATCTCAGCTGCGATGACAGC
TCAAGGTGCTGCAACAGTTGCTGTAGGATTAAAAACACGTAATGCGAAGCTTAAAGCTTTAGCGTTACCATCAGCATTCT
CAGCATTTTTAGGAATTACAGAGCCAGCAATCTTTGGTGTGAACTTACGTTACTTTAAACCATTTGTATGTGGATTGATT
GGTGGTGCCGTCGGTGGATTCTTAGCTTCATTATTACATCTAAAAGCATCAGGTATGGCAATTACAGTATTACCAGGTAC
TTTGTTATTTATTCAGGATATTGGACAATTACCATTCTACTTATTAGTACTTGCTGTATCGTTTGCAGTAGGATTTACAT
TGACATGGTTCTTTGGTTATAGCGATAAGATGGCTGAAGAGCTAAAGAAAGTAAAATGA

Upstream 100 bases:

>100_bases
TTCAGTAATAGTGTAGGTGCGCTCACTACTACGAATAAAGGGGCAATACAAAGTTTACCCGCGCTACTAGACATTCGACG
ATTAAAGGAGGAACAGTAAT

Downstream 100 bases:

>100_bases
TTATTTTTAAATAAATGGGCAATCGCCCGTTTATTTTTTTGGATGATTGTTAACTTATATTCAAAATTAGTTTACAATAT
AATAATGCAGTTATATTATA

Product: PTS system, trehalose-specific enzyme II, BC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAKTDKQIAQEVLDAVGGRENIASIAHCATRLRIMVNDKEKVNQEQVEDIEKVKGAFYNSGQYQIIFGTGTVNKIYDAIT
SLGIVGKSTDEVKTEGKKQGNAFQRAIRVFGDVFVPIIPVLVATGLFMGLRGVLMNEQILEWMGMTPKDISPNFILFTQV
LTDTAFAFLPALVAWSAFKVFGGSPVLGIVLGLMLVNPALPNAYAVGDGSAEALKFFGFIPVVGYQGSVLPAFFVGLIGA
KFEQLLRKRIPDAIDLIVTPFVTLLVMITLGLFVIGPVFHGLETIILTGTKWVLDLPYGIAGLIIGFFQQIIVVTGVHHI
FNFMEIQLLEEFKYNEFNPIISAAMTAQGAATVAVGLKTRNAKLKALALPSAFSAFLGITEPAIFGVNLRYFKPFVCGLI
GGAVGGFLASLLHLKASGMAITVLPGTLLFIQDIGQLPFYLLVLAVSFAVGFTLTWFFGYSDKMAEELKKVK

Sequences:

>Translated_472_residues
MAKTDKQIAQEVLDAVGGRENIASIAHCATRLRIMVNDKEKVNQEQVEDIEKVKGAFYNSGQYQIIFGTGTVNKIYDAIT
SLGIVGKSTDEVKTEGKKQGNAFQRAIRVFGDVFVPIIPVLVATGLFMGLRGVLMNEQILEWMGMTPKDISPNFILFTQV
LTDTAFAFLPALVAWSAFKVFGGSPVLGIVLGLMLVNPALPNAYAVGDGSAEALKFFGFIPVVGYQGSVLPAFFVGLIGA
KFEQLLRKRIPDAIDLIVTPFVTLLVMITLGLFVIGPVFHGLETIILTGTKWVLDLPYGIAGLIIGFFQQIIVVTGVHHI
FNFMEIQLLEEFKYNEFNPIISAAMTAQGAATVAVGLKTRNAKLKALALPSAFSAFLGITEPAIFGVNLRYFKPFVCGLI
GGAVGGFLASLLHLKASGMAITVLPGTLLFIQDIGQLPFYLLVLAVSFAVGFTLTWFFGYSDKMAEELKKVK
>Mature_471_residues
AKTDKQIAQEVLDAVGGRENIASIAHCATRLRIMVNDKEKVNQEQVEDIEKVKGAFYNSGQYQIIFGTGTVNKIYDAITS
LGIVGKSTDEVKTEGKKQGNAFQRAIRVFGDVFVPIIPVLVATGLFMGLRGVLMNEQILEWMGMTPKDISPNFILFTQVL
TDTAFAFLPALVAWSAFKVFGGSPVLGIVLGLMLVNPALPNAYAVGDGSAEALKFFGFIPVVGYQGSVLPAFFVGLIGAK
FEQLLRKRIPDAIDLIVTPFVTLLVMITLGLFVIGPVFHGLETIILTGTKWVLDLPYGIAGLIIGFFQQIIVVTGVHHIF
NFMEIQLLEEFKYNEFNPIISAAMTAQGAATVAVGLKTRNAKLKALALPSAFSAFLGITEPAIFGVNLRYFKPFVCGLIG
GAVGGFLASLLHLKASGMAITVLPGTLLFIQDIGQLPFYLLVLAVSFAVGFTLTWFFGYSDKMAEELKKVK

Specific function: Acts as both a kinase and a phosphatase on sacT [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=435, Percent_Identity=32.4137931034483, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790159, Length=431, Percent_Identity=30.6264501160093, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788769, Length=431, Percent_Identity=30.1624129930394, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI48994906, Length=491, Percent_Identity=27.6985743380855, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51093; Mature: 50962

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTDKQIAQEVLDAVGGRENIASIAHCATRLRIMVNDKEKVNQEQVEDIEKVKGAFYNS
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCHHHCCHHHHHHHHHHHHHHCCC
GQYQIIFGTGTVNKIYDAITSLGIVGKSTDEVKTEGKKQGNAFQRAIRVFGDVFVPIIPV
CCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LVATGLFMGLRGVLMNEQILEWMGMTPKDISPNFILFTQVLTDTAFAFLPALVAWSAFKV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FGGSPVLGIVLGLMLVNPALPNAYAVGDGSAEALKFFGFIPVVGYQGSVLPAFFVGLIGA
CCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KFEQLLRKRIPDAIDLIVTPFVTLLVMITLGLFVIGPVFHGLETIILTGTKWVLDLPYGI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHH
AGLIIGFFQQIIVVTGVHHIFNFMEIQLLEEFKYNEFNPIISAAMTAQGAATVAVGLKTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEEC
NAKLKALALPSAFSAFLGITEPAIFGVNLRYFKPFVCGLIGGAVGGFLASLLHLKASGMA
CCEEEEEECHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ITVLPGTLLFIQDIGQLPFYLLVLAVSFAVGFTLTWFFGYSDKMAEELKKVK
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKTDKQIAQEVLDAVGGRENIASIAHCATRLRIMVNDKEKVNQEQVEDIEKVKGAFYNS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCHHHCCHHHHHHHHHHHHHHCCC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EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3122206; 7934828; 9384377; 10568751 [H]