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The map label for this gene is fadH [C]

Identifier: 222150499

GI number: 222150499

Start: 249379

End: 250590

Strand: Direct

Name: fadH [C]

Synonym: MCCL_0249

Alternate gene names: 222150499

Gene position: 249379-250590 (Clockwise)

Preceding gene: 222150498

Following gene: 222150500

Centisome position: 11.86

GC content: 43.4

Gene sequence:

>1212_bases
ATGACAGTAGAGAGATTATTTGAACCACTTGAATTAAACAATGGTGTGACAATAAAGAATAGATTATATAAAGGAGCGAT
GAGTGAAGGGATGGCAGACGGTAACCACCGCCCAACGGAAGCCCTCGTTAATGCGTATCAGCACTGGGCTGATGGTGGTA
TCGGTATGGCGCTTACCGGCAATGTCATGGTCGATCGTCGTTATCTCGGTGAGCCGGGGAATGTAGTTGTAGAAGATGAG
CGAGATCTAGATATACTTAAGCGCTGGGCTGAAGCGGGTAAACGTAATAACAGTCATATCTGGATGCAGATTAATCATCC
CGGTAAGCAAAGTCCAAAGTCGGTATCTCAACAGCCTGTAGCTCCTAGCGCCATTCCAATTAATGGTGATGCTGGCAGTG
GATTTAATCCCCCGCGTGAAATGACATTGAATGAGATTAAAGAGACGATAGAACGCTTTGTAACTACTGCAGTAATCGCT
AAGAAAGCAGGATTTACAGGTGTTCAGATTCATGGTGCTCATGGGTATTTAGTGAGTCAGTTCTTATCTGCAAGGGATAA
TCGTCGCTTTGATGAATATGGAGGTACACTAGAAAATCGAATGAAGTTCTTAGTGGAAATCTATACAGGTATGCGCGAGG
CGTTAGGTAAAGATTTTCCGATCAGCTTAAAGATCAATTCAACGGACTTCAGTGACAAAGGCTTCTCCCTAGAGGATTCT
ATCACTGTAATCAAGAAGATGGATGAACTTGGAATCGATCATATTGAAATTTCAGGTGGAGATTATGAGAAACCGAAAAT
GATGGGTGACGGAGAAGTCTATTTCCTCGACTATGCAAAGAAAGTCAGTGAGAAAGTAGAGGTGCCGATTTCTGTGATTG
GAGGATTCCGTAGACAGCAAAGCATGGTTGATGCTATCACAGAGACGAATGTATCAATGATTGGTATTGGGCGTCCACTT
GTACTGAATCCTGAGCTTCCAAATCAGTTGCGTGAAGGAACGTATAAGGATATTACTTTACCAAGACTGACGACCAAGGT
TAAAGCCCTTGATAAAATAATCGGAGGCTTCGTTGGCTTAGCGTACTACGAACAGCAGATCAGAAGATTAGGCAATAACC
AGAAGCCTGTAGTGCATACGAATGCATGGAGTCCACTATTTGAGCTCGCTAAGACACATGGTGCGAAGGCGTTTTCGAAA
AGAAGAAGGTAG

Upstream 100 bases:

>100_bases
TGAATCATTCAGGAGGTGTTTTTTTCATGATTAATACGGTGCATTGAGGGAAGATACATAGTATGAAATGCTTTAAATTT
GAAGTGAAGGAGTAAATGAT

Downstream 100 bases:

>100_bases
ATGAAGAAGATTAAATTCACTATACCGGTTATAGAGGGATCAGATAGGGTGTGGACTAATACTATAGAATAGGGGGACTT
TTGATGAAGGTACTATTAAC

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTVERLFEPLELNNGVTIKNRLYKGAMSEGMADGNHRPTEALVNAYQHWADGGIGMALTGNVMVDRRYLGEPGNVVVEDE
RDLDILKRWAEAGKRNNSHIWMQINHPGKQSPKSVSQQPVAPSAIPINGDAGSGFNPPREMTLNEIKETIERFVTTAVIA
KKAGFTGVQIHGAHGYLVSQFLSARDNRRFDEYGGTLENRMKFLVEIYTGMREALGKDFPISLKINSTDFSDKGFSLEDS
ITVIKKMDELGIDHIEISGGDYEKPKMMGDGEVYFLDYAKKVSEKVEVPISVIGGFRRQQSMVDAITETNVSMIGIGRPL
VLNPELPNQLREGTYKDITLPRLTTKVKALDKIIGGFVGLAYYEQQIRRLGNNQKPVVHTNAWSPLFELAKTHGAKAFSK
RRR

Sequences:

>Translated_403_residues
MTVERLFEPLELNNGVTIKNRLYKGAMSEGMADGNHRPTEALVNAYQHWADGGIGMALTGNVMVDRRYLGEPGNVVVEDE
RDLDILKRWAEAGKRNNSHIWMQINHPGKQSPKSVSQQPVAPSAIPINGDAGSGFNPPREMTLNEIKETIERFVTTAVIA
KKAGFTGVQIHGAHGYLVSQFLSARDNRRFDEYGGTLENRMKFLVEIYTGMREALGKDFPISLKINSTDFSDKGFSLEDS
ITVIKKMDELGIDHIEISGGDYEKPKMMGDGEVYFLDYAKKVSEKVEVPISVIGGFRRQQSMVDAITETNVSMIGIGRPL
VLNPELPNQLREGTYKDITLPRLTTKVKALDKIIGGFVGLAYYEQQIRRLGNNQKPVVHTNAWSPLFELAKTHGAKAFSK
RRR
>Mature_402_residues
TVERLFEPLELNNGVTIKNRLYKGAMSEGMADGNHRPTEALVNAYQHWADGGIGMALTGNVMVDRRYLGEPGNVVVEDER
DLDILKRWAEAGKRNNSHIWMQINHPGKQSPKSVSQQPVAPSAIPINGDAGSGFNPPREMTLNEIKETIERFVTTAVIAK
KAGFTGVQIHGAHGYLVSQFLSARDNRRFDEYGGTLENRMKFLVEIYTGMREALGKDFPISLKINSTDFSDKGFSLEDSI
TVIKKMDELGIDHIEISGGDYEKPKMMGDGEVYFLDYAKKVSEKVEVPISVIGGFRRQQSMVDAITETNVSMIGIGRPLV
LNPELPNQLREGTYKDITLPRLTTKVKALDKIIGGFVGLAYYEQQIRRLGNNQKPVVHTNAWSPLFELAKTHGAKAFSKR
RR

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=372, Percent_Identity=28.2258064516129, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787939, Length=350, Percent_Identity=27.1428571428571, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17540738, Length=346, Percent_Identity=36.7052023121387, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17564188, Length=350, Percent_Identity=34.2857142857143, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17565138, Length=347, Percent_Identity=34.2939481268012, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=34.2857142857143, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17559804, Length=350, Percent_Identity=32.2857142857143, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI72001454, Length=349, Percent_Identity=33.2378223495702, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17566914, Length=350, Percent_Identity=32.2857142857143, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17566916, Length=358, Percent_Identity=29.608938547486, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321973, Length=209, Percent_Identity=27.7511961722488, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=212, Percent_Identity=28.3018867924528, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 44969; Mature: 44838

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVERLFEPLELNNGVTIKNRLYKGAMSEGMADGNHRPTEALVNAYQHWADGGIGMALTG
CCHHHHCCHHHCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEC
NVMVDRRYLGEPGNVVVEDERDLDILKRWAEAGKRNNSHIWMQINHPGKQSPKSVSQQPV
CEEEEHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCCCCC
APSAIPINGDAGSGFNPPREMTLNEIKETIERFVTTAVIAKKAGFTGVQIHGAHGYLVSQ
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
FLSARDNRRFDEYGGTLENRMKFLVEIYTGMREALGKDFPISLKINSTDFSDKGFSLEDS
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHH
ITVIKKMDELGIDHIEISGGDYEKPKMMGDGEVYFLDYAKKVSEKVEVPISVIGGFRRQQ
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHCCHHHHH
SMVDAITETNVSMIGIGRPLVLNPELPNQLREGTYKDITLPRLTTKVKALDKIIGGFVGL
HHHHHHHHCCEEEEECCCCEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AYYEQQIRRLGNNQKPVVHTNAWSPLFELAKTHGAKAFSKRRR
HHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
TVERLFEPLELNNGVTIKNRLYKGAMSEGMADGNHRPTEALVNAYQHWADGGIGMALTG
CHHHHCCHHHCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEC
NVMVDRRYLGEPGNVVVEDERDLDILKRWAEAGKRNNSHIWMQINHPGKQSPKSVSQQPV
CEEEEHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCCCCC
APSAIPINGDAGSGFNPPREMTLNEIKETIERFVTTAVIAKKAGFTGVQIHGAHGYLVSQ
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
FLSARDNRRFDEYGGTLENRMKFLVEIYTGMREALGKDFPISLKINSTDFSDKGFSLEDS
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHH
ITVIKKMDELGIDHIEISGGDYEKPKMMGDGEVYFLDYAKKVSEKVEVPISVIGGFRRQQ
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHCCHHHHH
SMVDAITETNVSMIGIGRPLVLNPELPNQLREGTYKDITLPRLTTKVKALDKIIGGFVGL
HHHHHHHHCCEEEEECCCCEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AYYEQQIRRLGNNQKPVVHTNAWSPLFELAKTHGAKAFSKRRR
HHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]