Click here to switch to the map view.

The map label for this gene is azoR

Identifier: 222150356

GI number: 222150356

Start: 110542

End: 111177

Strand: Direct

Name: azoR

Synonym: MCCL_0106

Alternate gene names: 222150356

Gene position: 110542-111177 (Clockwise)

Preceding gene: 222150355

Following gene: 222150358

Centisome position: 5.26

GC content: 35.85

Gene sequence:

>636_bases
ATGACAAAAGTACTATATATTACAGGTCATCCAAACGATGAAACAGTTTCAAATTCTATGGCAGCAGGTAAATCATTTAT
CGAAAGCTATAAACAATCAAATCCAGATCACGAAGTTGTACATATTGATCTTTATGACACATTCATTCCATTAATCGATA
AAGAAGTATTTGATGGTTGGGGTAAATTACAATCAGGTAAAGGATTCGAAGCATTATCTGAAACAGAACAACAAAAAGTG
GCTCGTTTAAATGAATTAAGTGACGAATTTGCAGCAGCAGATAAATATATATTCGTTACACCAATGTGGAACCTGTCATT
CCCAGCAGTAGTTAAAGCATATATCGATGCTGTCGCCGTAGCAGGAAAAGCTTTCAAATACACAGCTGAAGGTGCTGTTG
GTTTATTAACAGACAAAAAAGCGTTACTTATTCAGTCACGTGGTGGTATTTATTCAGAAGGACCAGCAGCAGATTTTGAA
TTAGGTAACCGTTACTTACAAACAATTCTTGGATTCTTTGGTGTACCAAGTGTTGAAGAATTAGTTATCGAAGGTCATAA
CCAAATGCCAGAGAAAGCAGAAGAAATTAAAGCAGACGGTGTACGTCGTGCTGAAGCATTAGGAAAAACATTTTAA

Upstream 100 bases:

>100_bases
AATAAATTTTATACTTTGAAATTAATCTTTTGATTTTATTATATTATATGGTATATTAAATATATCGAGTATATTAATTA
AATCAAAGGAGTAATTTATT

Downstream 100 bases:

>100_bases
TATAAACGAATGAAGCGGAGAGATTATTGTAATCTCTTCGCTTTTTACGTTCCACAAAAAGTTTGATAGGGTTTATCTTT
CGGACCAGGATGTTTATATT

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKVLYITGHPNDETVSNSMAAGKSFIESYKQSNPDHEVVHIDLYDTFIPLIDKEVFDGWGKLQSGKGFEALSETEQQKV
ARLNELSDEFAAADKYIFVTPMWNLSFPAVVKAYIDAVAVAGKAFKYTAEGAVGLLTDKKALLIQSRGGIYSEGPAADFE
LGNRYLQTILGFFGVPSVEELVIEGHNQMPEKAEEIKADGVRRAEALGKTF

Sequences:

>Translated_211_residues
MTKVLYITGHPNDETVSNSMAAGKSFIESYKQSNPDHEVVHIDLYDTFIPLIDKEVFDGWGKLQSGKGFEALSETEQQKV
ARLNELSDEFAAADKYIFVTPMWNLSFPAVVKAYIDAVAVAGKAFKYTAEGAVGLLTDKKALLIQSRGGIYSEGPAADFE
LGNRYLQTILGFFGVPSVEELVIEGHNQMPEKAEEIKADGVRRAEALGKTF
>Mature_210_residues
TKVLYITGHPNDETVSNSMAAGKSFIESYKQSNPDHEVVHIDLYDTFIPLIDKEVFDGWGKLQSGKGFEALSETEQQKVA
RLNELSDEFAAADKYIFVTPMWNLSFPAVVKAYIDAVAVAGKAFKYTAEGAVGLLTDKKALLIQSRGGIYSEGPAADFEL
GNRYLQTILGFFGVPSVEELVIEGHNQMPEKAEEIKADGVRRAEALGKTF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=175, Percent_Identity=35.4285714285714, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_MACCJ (B9E9A2)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559509.1
- ProteinModelPortal:   B9E9A2
- GeneID:   7390868
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0106
- OMA:   FSGWGKL
- ProtClustDB:   PRK00170
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 23188; Mature: 23057

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLYITGHPNDETVSNSMAAGKSFIESYKQSNPDHEVVHIDLYDTFIPLIDKEVFDGW
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHH
GKLQSGKGFEALSETEQQKVARLNELSDEFAAADKYIFVTPMWNLSFPAVVKAYIDAVAV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
AGKAFKYTAEGAVGLLTDKKALLIQSRGGIYSEGPAADFELGNRYLQTILGFFGVPSVEE
HCHHEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
LVIEGHNQMPEKAEEIKADGVRRAEALGKTF
HHHCCCCCCHHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
TKVLYITGHPNDETVSNSMAAGKSFIESYKQSNPDHEVVHIDLYDTFIPLIDKEVFDGW
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHH
GKLQSGKGFEALSETEQQKVARLNELSDEFAAADKYIFVTPMWNLSFPAVVKAYIDAVAV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
AGKAFKYTAEGAVGLLTDKKALLIQSRGGIYSEGPAADFELGNRYLQTILGFFGVPSVEE
HCHHEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
LVIEGHNQMPEKAEEIKADGVRRAEALGKTF
HHHCCCCCCHHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA