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The map label for this gene is recF

Identifier: 222150257

GI number: 222150257

Start: 6785

End: 7894

Strand: Direct

Name: recF

Synonym: MCCL_0007

Alternate gene names: 222150257

Gene position: 6785-7894 (Clockwise)

Preceding gene: 222150256

Following gene: 222150258

Centisome position: 0.32

GC content: 33.69

Gene sequence:

>1110_bases
ATGAAATTAAAGACGTTAACGCTAACTCATTATCGAAATTATGAAACTGCGGAATTAAACTTTAGTGATGAAGTAAATAT
TTTCATAGGCATTAATGCACAAGGAAAAACAAACTTACTTGAAGCAATTTATTGCCTAGCGATGGCTAAAAGCCATCGCA
CCTCTAATGATAAAGAACTGATAGGCTGGGGACATGAATTCAGTCATATCGAAGGTATGTTGTCTTACAAGCATGGCACT
ATGCCCTTATCTTTATCTATCTCTAAAAAAGGAAAAAAAGCTAAAGTAAACTATTTAGAACAGAAACGATTAACAGAATA
TATTGGACATATGAATGTTGTACTCTTTGCTCCTGAAGATTTGAATCTGGTCAAAGGGAGTCCGCAGATAAGAAGAAGAT
TTATTGACATGGAAATAGGTCAGATATCTGCCGTTTACTTAAATGATTTATCAAATTATCAGCGATTATTAAAGCAGAAA
AACCATTTGTTAAAGCAAATGAAATTATCAAATAGTAATGATATGACAATGCTTGAAGTTATCAATGAACAGTTTGCTCA
ATATGCAGTGAAATTAACATTGAGAAGAAAAATGTTTATCGAACAGCTAGAAACTTTAGCAATACCGATTCATACAGGTA
TTACAAAAGATAAAGAACGTCTCTCTTTACGATACAATGCAAGCTTGAACTATGAATTAGCTGAGCAGGAGATGTTTGAA
GAAACGATTCGTATATTAAATGCGAATATGGGCAAGGAGATTGAAAGAACACAAAGCTTATATGGACCACACCGTGACGA
CTTAAGCTTTAAAATAAATGATATCGATGTACAGACATATGGTTCGCAAGGCCAGCAGCGTACAACTGCATTATCTATAA
AATTAGCAGAGATTGAACTTATTAACCAGGAAATTGGAGAGTATCCAATATTATTACTGGATGACGTACTAAGTGAGTTG
GATGATGATAGACAGACACACCTGTTAACAACAATACAACATAAAGTTCAAACATTTGTGACGACAACGTCAGTAGAAGG
TATTGAACACGAAACAATCAATAAAGCGAAATTATTCAATGTATCAGAAGGACAGATTTCGACTAACTAA

Upstream 100 bases:

>100_bases
TAAACGGAGAACCGGAAAATAGACGTGGCAAGAAGTTGCTTCAGGATGATTTGCTGGAAGTTGAAGGTGAAACATATCAG
ATCACGTATAAGACGACAGA

Downstream 100 bases:

>100_bases
AGGATAGGTGAACATAGTGGAACAACAAAATTTAGATCAGTATGGTGCCGATCAGATACAGGTACTAGAAGGATTAGAAG
CGGTAAGAAAACGTCCTGGT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKLKTLTLTHYRNYETAELNFSDEVNIFIGINAQGKTNLLEAIYCLAMAKSHRTSNDKELIGWGHEFSHIEGMLSYKHGT
MPLSLSISKKGKKAKVNYLEQKRLTEYIGHMNVVLFAPEDLNLVKGSPQIRRRFIDMEIGQISAVYLNDLSNYQRLLKQK
NHLLKQMKLSNSNDMTMLEVINEQFAQYAVKLTLRRKMFIEQLETLAIPIHTGITKDKERLSLRYNASLNYELAEQEMFE
ETIRILNANMGKEIERTQSLYGPHRDDLSFKINDIDVQTYGSQGQQRTTALSIKLAEIELINQEIGEYPILLLDDVLSEL
DDDRQTHLLTTIQHKVQTFVTTTSVEGIEHETINKAKLFNVSEGQISTN

Sequences:

>Translated_369_residues
MKLKTLTLTHYRNYETAELNFSDEVNIFIGINAQGKTNLLEAIYCLAMAKSHRTSNDKELIGWGHEFSHIEGMLSYKHGT
MPLSLSISKKGKKAKVNYLEQKRLTEYIGHMNVVLFAPEDLNLVKGSPQIRRRFIDMEIGQISAVYLNDLSNYQRLLKQK
NHLLKQMKLSNSNDMTMLEVINEQFAQYAVKLTLRRKMFIEQLETLAIPIHTGITKDKERLSLRYNASLNYELAEQEMFE
ETIRILNANMGKEIERTQSLYGPHRDDLSFKINDIDVQTYGSQGQQRTTALSIKLAEIELINQEIGEYPILLLDDVLSEL
DDDRQTHLLTTIQHKVQTFVTTTSVEGIEHETINKAKLFNVSEGQISTN
>Mature_369_residues
MKLKTLTLTHYRNYETAELNFSDEVNIFIGINAQGKTNLLEAIYCLAMAKSHRTSNDKELIGWGHEFSHIEGMLSYKHGT
MPLSLSISKKGKKAKVNYLEQKRLTEYIGHMNVVLFAPEDLNLVKGSPQIRRRFIDMEIGQISAVYLNDLSNYQRLLKQK
NHLLKQMKLSNSNDMTMLEVINEQFAQYAVKLTLRRKMFIEQLETLAIPIHTGITKDKERLSLRYNASLNYELAEQEMFE
ETIRILNANMGKEIERTQSLYGPHRDDLSFKINDIDVQTYGSQGQQRTTALSIKLAEIELINQEIGEYPILLLDDVLSEL
DDDRQTHLLTTIQHKVQTFVTTTSVEGIEHETINKAKLFNVSEGQISTN

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=372, Percent_Identity=27.4193548387097, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_MACCJ (B9E903)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559410.1
- GeneID:   7390558
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0007
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42445; Mature: 42445

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTLTLTHYRNYETAELNFSDEVNIFIGINAQGKTNLLEAIYCLAMAKSHRTSNDKEL
CCEEEEEEEEECCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEE
IGWGHEFSHIEGMLSYKHGTMPLSLSISKKGKKAKVNYLEQKRLTEYIGHMNVVLFAPED
EECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LNLVKGSPQIRRRFIDMEIGQISAVYLNDLSNYQRLLKQKNHLLKQMKLSNSNDMTMLEV
CCEECCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
INEQFAQYAVKLTLRRKMFIEQLETLAIPIHTGITKDKERLSLRYNASLNYELAEQEMFE
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHEEEEECCCCCCCHHHHHHHH
ETIRILNANMGKEIERTQSLYGPHRDDLSFKINDIDVQTYGSQGQQRTTALSIKLAEIEL
HHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEEEEHHHHH
INQEIGEYPILLLDDVLSELDDDRQTHLLTTIQHKVQTFVTTTSVEGIEHETINKAKLFN
HHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEE
VSEGQISTN
CCCCCCCCC
>Mature Secondary Structure
MKLKTLTLTHYRNYETAELNFSDEVNIFIGINAQGKTNLLEAIYCLAMAKSHRTSNDKEL
CCEEEEEEEEECCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEE
IGWGHEFSHIEGMLSYKHGTMPLSLSISKKGKKAKVNYLEQKRLTEYIGHMNVVLFAPED
EECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LNLVKGSPQIRRRFIDMEIGQISAVYLNDLSNYQRLLKQKNHLLKQMKLSNSNDMTMLEV
CCEECCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
INEQFAQYAVKLTLRRKMFIEQLETLAIPIHTGITKDKERLSLRYNASLNYELAEQEMFE
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHEEEEECCCCCCCHHHHHHHH
ETIRILNANMGKEIERTQSLYGPHRDDLSFKINDIDVQTYGSQGQQRTTALSIKLAEIEL
HHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEEEEHHHHH
INQEIGEYPILLLDDVLSELDDDRQTHLLTTIQHKVQTFVTTTSVEGIEHETINKAKLFN
HHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEE
VSEGQISTN
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA