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The map label for this gene is gyrB [H]

Identifier: 222150258

GI number: 222150258

Start: 7902

End: 9830

Strand: Direct

Name: gyrB [H]

Synonym: MCCL_0008

Alternate gene names: 222150258

Gene position: 7902-9830 (Clockwise)

Preceding gene: 222150257

Following gene: 222150259

Centisome position: 0.38

GC content: 37.9

Gene sequence:

>1929_bases
GTGAACATAGTGGAACAACAAAATTTAGATCAGTATGGTGCCGATCAGATACAGGTACTAGAAGGATTAGAAGCGGTAAG
AAAACGTCCTGGTATGTATATTGGTTCTACCGCATCAAAAGGGTTACACCATCTTGTCTGGGAAATTGTTGATAATAGTA
TTGACGAAGCCCTTGCTGGATATGCAGATCATGTTGAAGTGACTATAGAAGAAGATAACTGGATAAAAGTTACGGATAAT
GGTCGTGGTATTCCGGTAGATATTCAGAAAAAAATGGGTCGTCCTGCGGTTGAAGTCATTTTAACTGTACTACATGCTGG
TGGTAAATTTGGGGGCGGCGGATATAAAGTTTCGGGGGGACTGCATGGCGTTGGTTCATCAGTTGTTAATGCCCTATCGT
CTACCTTAGAAGTATATGTTCATAAAGATGGCAAGATTTATCATCAGGCATATCACCGTGGTGTACCTGCATTTGATTTA
AAGGTGATTGGAGAAACAGATAAGACTGGAACTGTCATTCGTTTTAAAGCAGATTCAGAAATATTTACTGAAACAACAGA
ATATGAATACGAAATTCTGCAGAAACGTATTAGAGAATTAGCGTTCTTAAATAAAGGTATCAAACTTGAAATTAAAGATG
AACGTGAACAGGATGAAATTCGTCATGACGTGTACCATTATGAAGGTGGTATTAAATCATATGTAGAGTTGTTAAATAAG
TCTAAAGAAACTTTATTCCCGGAACCGATTTATATTCATGATAATCGTGATGAGATTGAAGTCGAGATTTCAATTCAATA
TAACAATGGCTATTCAACAAATCTTCTAAGCTATGCGAATAATATTCATACATACGAAGGTGGTACGCATGAAGATGGAT
TCAAGCGTGCATTAACACGTGTGATTAATAGTTATGGTGTTAAGAATAAATTGATTAAAGAAGCCGATGAGAAGTTATCT
GGAGAAGATGTACGTGAGGGAATTACAGCAATCGTCTCCATTAAGCACGGAAACCCGCAATTTGAAGGACAGACGAAAAC
AAAGCTTGGGAATACAGAAGTACGTCAAATTACTGATAACTTATTTGCAGAAAACTTTGAACGTTTCTTATTAGAAAATC
CTCCTATTGCACGTATTATCGTGGAAAAAGGGATTATGGCATCGCGTGCTCGACTTGCAGCAAAGAAAGCGCGTGAAGTG
ACACGTCGTAAGTCGGGACTTGAAATTTCTAGTCTTCCAGGAAAGCTTGCAGATTGTTCAAGCAAAGACCCATCCATTAG
TGAATTATACATTGTTGAGGGAGATTCTGCCGGTGGTTCAGCGAAGTCAGGAAGAGACTCTAAGACACAAGCAATTTTAC
CGTTACGTGGTAAAATTTTAAATGTTGAAAAAGCAAGGCTTGATCGAATTCTTGGTAATAATGAAATTCGTTCTATGATT
ACAGCAATGGGCACAGGTATTGGTGGCGAGTTTGATATTTCAAAAGCACGCTATCATAAAATTGTTATTATGACAGATGC
AGATGTAGACGGGGCTCACATTAGAACATTGTTGCTTACATTCTTCTATCGATTTATGAGACCTCTACTAGAAGCAGGAT
ATGTTTATATCGCACAACCACCATTATTTAAGGTGGAACAAGGTAAGAAAAAATATTATGTATATAACGAACGTGAACTT
GATAAATTGAGAAAAGAATTACCGACAACACCGAAATGGCAACTTGCAAGATATAAAGGTTTAGGAGAGATGAATGCGGA
TCAGCTATGGGAAACCACTATGAATCCAGAACATCGTTCGATGCTACAAGTGACACTAAGTGATGCGATAGAAGCGGATG
AAGTTTTCGAAATGCTGATGGGAGATGTTGTAGAACATCGTCGTACGTTCATTGAAGAGAATGCAGAATACGCAAACGTT
GATGTATAA

Upstream 100 bases:

>100_bases
GTGACGACAACGTCAGTAGAAGGTATTGAACACGAAACAATCAATAAAGCGAAATTATTCAATGTATCAGAAGGACAGAT
TTCGACTAACTAAAGGATAG

Downstream 100 bases:

>100_bases
AGATAGTATTTTAAGGAGGCGCCATCTATGGCTTTAGACCATGATTCAAAATTGAAGGAGCAAAATATCGTCAAAGAAAT
GAAAGATTCATTTCTAGACT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MNIVEQQNLDQYGADQIQVLEGLEAVRKRPGMYIGSTASKGLHHLVWEIVDNSIDEALAGYADHVEVTIEEDNWIKVTDN
GRGIPVDIQKKMGRPAVEVILTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSSTLEVYVHKDGKIYHQAYHRGVPAFDL
KVIGETDKTGTVIRFKADSEIFTETTEYEYEILQKRIRELAFLNKGIKLEIKDEREQDEIRHDVYHYEGGIKSYVELLNK
SKETLFPEPIYIHDNRDEIEVEISIQYNNGYSTNLLSYANNIHTYEGGTHEDGFKRALTRVINSYGVKNKLIKEADEKLS
GEDVREGITAIVSIKHGNPQFEGQTKTKLGNTEVRQITDNLFAENFERFLLENPPIARIIVEKGIMASRARLAAKKAREV
TRRKSGLEISSLPGKLADCSSKDPSISELYIVEGDSAGGSAKSGRDSKTQAILPLRGKILNVEKARLDRILGNNEIRSMI
TAMGTGIGGEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLLEAGYVYIAQPPLFKVEQGKKKYYVYNEREL
DKLRKELPTTPKWQLARYKGLGEMNADQLWETTMNPEHRSMLQVTLSDAIEADEVFEMLMGDVVEHRRTFIEENAEYANV
DV

Sequences:

>Translated_642_residues
MNIVEQQNLDQYGADQIQVLEGLEAVRKRPGMYIGSTASKGLHHLVWEIVDNSIDEALAGYADHVEVTIEEDNWIKVTDN
GRGIPVDIQKKMGRPAVEVILTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSSTLEVYVHKDGKIYHQAYHRGVPAFDL
KVIGETDKTGTVIRFKADSEIFTETTEYEYEILQKRIRELAFLNKGIKLEIKDEREQDEIRHDVYHYEGGIKSYVELLNK
SKETLFPEPIYIHDNRDEIEVEISIQYNNGYSTNLLSYANNIHTYEGGTHEDGFKRALTRVINSYGVKNKLIKEADEKLS
GEDVREGITAIVSIKHGNPQFEGQTKTKLGNTEVRQITDNLFAENFERFLLENPPIARIIVEKGIMASRARLAAKKAREV
TRRKSGLEISSLPGKLADCSSKDPSISELYIVEGDSAGGSAKSGRDSKTQAILPLRGKILNVEKARLDRILGNNEIRSMI
TAMGTGIGGEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLLEAGYVYIAQPPLFKVEQGKKKYYVYNEREL
DKLRKELPTTPKWQLARYKGLGEMNADQLWETTMNPEHRSMLQVTLSDAIEADEVFEMLMGDVVEHRRTFIEENAEYANV
DV
>Mature_642_residues
MNIVEQQNLDQYGADQIQVLEGLEAVRKRPGMYIGSTASKGLHHLVWEIVDNSIDEALAGYADHVEVTIEEDNWIKVTDN
GRGIPVDIQKKMGRPAVEVILTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSSTLEVYVHKDGKIYHQAYHRGVPAFDL
KVIGETDKTGTVIRFKADSEIFTETTEYEYEILQKRIRELAFLNKGIKLEIKDEREQDEIRHDVYHYEGGIKSYVELLNK
SKETLFPEPIYIHDNRDEIEVEISIQYNNGYSTNLLSYANNIHTYEGGTHEDGFKRALTRVINSYGVKNKLIKEADEKLS
GEDVREGITAIVSIKHGNPQFEGQTKTKLGNTEVRQITDNLFAENFERFLLENPPIARIIVEKGIMASRARLAAKKAREV
TRRKSGLEISSLPGKLADCSSKDPSISELYIVEGDSAGGSAKSGRDSKTQAILPLRGKILNVEKARLDRILGNNEIRSMI
TAMGTGIGGEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLLEAGYVYIAQPPLFKVEQGKKKYYVYNEREL
DKLRKELPTTPKWQLARYKGLGEMNADQLWETTMNPEHRSMLQVTLSDAIEADEVFEMLMGDVVEHRRTFIEENAEYANV
DV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=627, Percent_Identity=26.4752791068581, Blast_Score=136, Evalue=8e-32,
Organism=Homo sapiens, GI19913408, Length=642, Percent_Identity=26.3239875389408, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=54.7747747747748, Blast_Score=600, Evalue=1e-172,
Organism=Escherichia coli, GI1789408, Length=640, Percent_Identity=39.21875, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=606, Percent_Identity=25.2475247524752, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=555, Percent_Identity=23.4234234234234, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=180, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=653, Percent_Identity=27.5650842266462, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17136538, Length=618, Percent_Identity=25.4045307443366, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72309; Mature: 72309

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00154 ATPASE_E1_E2 ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVEQQNLDQYGADQIQVLEGLEAVRKRPGMYIGSTASKGLHHLVWEIVDNSIDEALAG
CCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHC
YADHVEVTIEEDNWIKVTDNGRGIPVDIQKKMGRPAVEVILTVLHAGGKFGGGGYKVSGG
CCCCEEEEEECCCEEEECCCCCCCCEEHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGSSVVNALSSTLEVYVHKDGKIYHQAYHRGVPAFDLKVIGETDKTGTVIRFKADSE
CCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCC
IFTETTEYEYEILQKRIRELAFLNKGIKLEIKDEREQDEIRHDVYHYEGGIKSYVELLNK
HHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHC
SKETLFPEPIYIHDNRDEIEVEISIQYNNGYSTNLLSYANNIHTYEGGTHEDGFKRALTR
CCHHCCCCCEEEECCCCEEEEEEEEEECCCCCHHHHHHHCCCEECCCCCCCHHHHHHHHH
VINSYGVKNKLIKEADEKLSGEDVREGITAIVSIKHGNPQFEGQTKTKLGNTEVRQITDN
HHHHCCHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHH
LFAENFERFLLENPPIARIIVEKGIMASRARLAAKKAREVTRRKSGLEISSLPGKLADCS
HHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHCCC
SKDPSISELYIVEGDSAGGSAKSGRDSKTQAILPLRGKILNVEKARLDRILGNNEIRSMI
CCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHCCHHHHHHH
TAMGTGIGGEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLLEAGYVYIAQP
HHHHCCCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLFKVEQGKKKYYVYNERELDKLRKELPTTPKWQLARYKGLGEMNADQLWETTMNPEHRS
CCEEECCCCCEEEEECHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCHHH
MLQVTLSDAIEADEVFEMLMGDVVEHRRTFIEENAEYANVDV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNIVEQQNLDQYGADQIQVLEGLEAVRKRPGMYIGSTASKGLHHLVWEIVDNSIDEALAG
CCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHC
YADHVEVTIEEDNWIKVTDNGRGIPVDIQKKMGRPAVEVILTVLHAGGKFGGGGYKVSGG
CCCCEEEEEECCCEEEECCCCCCCCEEHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGSSVVNALSSTLEVYVHKDGKIYHQAYHRGVPAFDLKVIGETDKTGTVIRFKADSE
CCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCC
IFTETTEYEYEILQKRIRELAFLNKGIKLEIKDEREQDEIRHDVYHYEGGIKSYVELLNK
HHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHC
SKETLFPEPIYIHDNRDEIEVEISIQYNNGYSTNLLSYANNIHTYEGGTHEDGFKRALTR
CCHHCCCCCEEEECCCCEEEEEEEEEECCCCCHHHHHHHCCCEECCCCCCCHHHHHHHHH
VINSYGVKNKLIKEADEKLSGEDVREGITAIVSIKHGNPQFEGQTKTKLGNTEVRQITDN
HHHHCCHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHH
LFAENFERFLLENPPIARIIVEKGIMASRARLAAKKAREVTRRKSGLEISSLPGKLADCS
HHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHCCC
SKDPSISELYIVEGDSAGGSAKSGRDSKTQAILPLRGKILNVEKARLDRILGNNEIRSMI
CCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHCCHHHHHHH
TAMGTGIGGEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLLEAGYVYIAQP
HHHHCCCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLFKVEQGKKKYYVYNERELDKLRKELPTTPKWQLARYKGLGEMNADQLWETTMNPEHRS
CCEEECCCCCEEEEECHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCHHH
MLQVTLSDAIEADEVFEMLMGDVVEHRRTFIEENAEYANVDV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA