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The map label for this gene is livJ [H]

Identifier: 222111202

GI number: 222111202

Start: 2147505

End: 2148638

Strand: Direct

Name: livJ [H]

Synonym: Dtpsy_2010

Alternate gene names: 222111202

Gene position: 2147505-2148638 (Clockwise)

Preceding gene: 222111201

Following gene: 222111203

Centisome position: 56.56

GC content: 69.14

Gene sequence:

>1134_bases
ATGAACCAGAAACCCCATGGGCGCCTGCGCGCCTGGCAAGCCGCCTGCCTGCTGGCGGGCGCCCTGAGCGGCGGCGCCGC
CTCTGCCGACATCCTGATCGGGCAGACCACGGCCGTCAGCGGCCCAGTGGCGGCCAGCGTGGGCGAGACCGTCGCGGGCG
TGCACCTGTATCTGGACCACGTCAATGCCAAGGGCGGCGTGCATGGCGAACGCATCAAGCTGATCACGCTGGACGACAAG
TTCGAGCCCAAGCTCGCCGGCGAGAACGCGCGCCGCCTGATCGAAGACGACAACGTGGCCGCGCTGTTCATGAGCCGCGC
CACGCCGCACACCGAAGCCATCCTGCCGCACCTGGCCAAGCACGGCGTGGCGCTGGTGGCGCCCTCCACGGGTGCGATGA
CCTTCCACAAGCCCGCCAACCCGCTGGTGTTCAACGTGCGCTCCAGCTACCAGGCCGAGACCGAGAAGGCCGTGGAGCAC
CTGCACACCATCATGGTCCAGCGCATCGCCGTGGTGCATGTGGACGACAGCTTTGGCGAGGACTGCCTGGCCGGTGCCCA
GAAGGGCTTTGCCAACGTGGGCAGCAAGCCCGTGGCCGTGATCAAGACCGACCGCGCCAAGCCGGACTACGCCACCATCG
TGCCGCAGCTCATCCAGGCGCAGGCCCAGGCGGTCATCTGGATCGGCTCCAGCGTGGCGGTGAGCGACGGCGTGAAAGCG
CTGCGCAAGGCCGGCTCGTCCATGCAGGTGGTCACGGTGTCGAACAACGCATCCCGCGGCTTCGTCGCCCAGCTGGGCGA
CTCGGTGCGCGGCGTGATCGTCACGCAGGTGTTCCCCAACGTGCGCTCCATGGGCTACGCCATCAACCGCGAGGCCGCCG
CTCTGGCCAAGGCCAAGGGCAACCTGACGCTGTCACCCTCGGTGATGGAAGGCTTCACCTCCGCCAAGGTGCTGGTGGAA
GGCCTGCGCCGCGCCGGCCCCAAGCCCACGCGCGAGAAGATCGTCACCGCGCTGGGCGGCATGAGCCACTACGACCTGGG
CGGGCTGGAGCTGGGCTACGGCCCCGGCGACCGCACGGGCCTCAGCTACGTGGAGCTGTCCATCATCGACGCGGACGGGC
TGTTCCGCCGCTGA

Upstream 100 bases:

>100_bases
GTCACAATATGCACCTTTTGAGGGACTGCAGCTACCCGCTGCCAGTGCGAAACCACAGCCCATGCACCCACTTGGTGCAA
CGCGGGCACAGGGAGGAACC

Downstream 100 bases:

>100_bases
CGCCGTCGCTGCCGCGCCGCGGGGCCACCGGGGCCACCGGGCCACCGGGCTACCGGGTCACCGCCCCTCGCGCGCCGCGG
CAGCCCCCATCGCCAATCGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNQKPHGRLRAWQAACLLAGALSGGAASADILIGQTTAVSGPVAASVGETVAGVHLYLDHVNAKGGVHGERIKLITLDDK
FEPKLAGENARRLIEDDNVAALFMSRATPHTEAILPHLAKHGVALVAPSTGAMTFHKPANPLVFNVRSSYQAETEKAVEH
LHTIMVQRIAVVHVDDSFGEDCLAGAQKGFANVGSKPVAVIKTDRAKPDYATIVPQLIQAQAQAVIWIGSSVAVSDGVKA
LRKAGSSMQVVTVSNNASRGFVAQLGDSVRGVIVTQVFPNVRSMGYAINREAAALAKAKGNLTLSPSVMEGFTSAKVLVE
GLRRAGPKPTREKIVTALGGMSHYDLGGLELGYGPGDRTGLSYVELSIIDADGLFRR

Sequences:

>Translated_377_residues
MNQKPHGRLRAWQAACLLAGALSGGAASADILIGQTTAVSGPVAASVGETVAGVHLYLDHVNAKGGVHGERIKLITLDDK
FEPKLAGENARRLIEDDNVAALFMSRATPHTEAILPHLAKHGVALVAPSTGAMTFHKPANPLVFNVRSSYQAETEKAVEH
LHTIMVQRIAVVHVDDSFGEDCLAGAQKGFANVGSKPVAVIKTDRAKPDYATIVPQLIQAQAQAVIWIGSSVAVSDGVKA
LRKAGSSMQVVTVSNNASRGFVAQLGDSVRGVIVTQVFPNVRSMGYAINREAAALAKAKGNLTLSPSVMEGFTSAKVLVE
GLRRAGPKPTREKIVTALGGMSHYDLGGLELGYGPGDRTGLSYVELSIIDADGLFRR
>Mature_377_residues
MNQKPHGRLRAWQAACLLAGALSGGAASADILIGQTTAVSGPVAASVGETVAGVHLYLDHVNAKGGVHGERIKLITLDDK
FEPKLAGENARRLIEDDNVAALFMSRATPHTEAILPHLAKHGVALVAPSTGAMTFHKPANPLVFNVRSSYQAETEKAVEH
LHTIMVQRIAVVHVDDSFGEDCLAGAQKGFANVGSKPVAVIKTDRAKPDYATIVPQLIQAQAQAVIWIGSSVAVSDGVKA
LRKAGSSMQVVTVSNNASRGFVAQLGDSVRGVIVTQVFPNVRSMGYAINREAAALAKAKGNLTLSPSVMEGFTSAKVLVE
GLRRAGPKPTREKIVTALGGMSHYDLGGLELGYGPGDRTGLSYVELSIIDADGLFRR

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=268, Percent_Identity=23.5074626865672, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789867, Length=264, Percent_Identity=21.969696969697, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39605; Mature: 39605

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKPHGRLRAWQAACLLAGALSGGAASADILIGQTTAVSGPVAASVGETVAGVHLYLDH
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHCCHHHEEEEEEEE
VNAKGGVHGERIKLITLDDKFEPKLAGENARRLIEDDNVAALFMSRATPHTEAILPHLAK
ECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
HGVALVAPSTGAMTFHKPANPLVFNVRSSYQAETEKAVEHLHTIMVQRIAVVHVDDSFGE
CCEEEEECCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
DCLAGAQKGFANVGSKPVAVIKTDRAKPDYATIVPQLIQAQAQAVIWIGSSVAVSDGVKA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
LRKAGSSMQVVTVSNNASRGFVAQLGDSVRGVIVTQVFPNVRSMGYAINREAAALAKAKG
HHHCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEHHHCCHHHHCEECCCHHHHHHHCCC
NLTLSPSVMEGFTSAKVLVEGLRRAGPKPTREKIVTALGGMSHYDLGGLELGYGPGDRTG
CEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
LSYVELSIIDADGLFRR
CEEEEEEEECCCCCCCC
>Mature Secondary Structure
MNQKPHGRLRAWQAACLLAGALSGGAASADILIGQTTAVSGPVAASVGETVAGVHLYLDH
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHCCHHHEEEEEEEE
VNAKGGVHGERIKLITLDDKFEPKLAGENARRLIEDDNVAALFMSRATPHTEAILPHLAK
ECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
HGVALVAPSTGAMTFHKPANPLVFNVRSSYQAETEKAVEHLHTIMVQRIAVVHVDDSFGE
CCEEEEECCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
DCLAGAQKGFANVGSKPVAVIKTDRAKPDYATIVPQLIQAQAQAVIWIGSSVAVSDGVKA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
LRKAGSSMQVVTVSNNASRGFVAQLGDSVRGVIVTQVFPNVRSMGYAINREAAALAKAKG
HHHCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEHHHCCHHHHCEECCCHHHHHHHCCC
NLTLSPSVMEGFTSAKVLVEGLRRAGPKPTREKIVTALGGMSHYDLGGLELGYGPGDRTG
CEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
LSYVELSIIDADGLFRR
CEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA