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The map label for this gene is waaA [H]

Identifier: 222111024

GI number: 222111024

Start: 1948804

End: 1950165

Strand: Direct

Name: waaA [H]

Synonym: Dtpsy_1831

Alternate gene names: 222111024

Gene position: 1948804-1950165 (Clockwise)

Preceding gene: 222111023

Following gene: 222111025

Centisome position: 51.33

GC content: 72.76

Gene sequence:

>1362_bases
ATGCACCAGCTCATCCTGTGGCTCTACAGCCTGGCCGTGTGGCTGGCCACGCCGCTGCTGCTGCGCAAGCTGCGCCGCCG
CGCGCTCACCGAGCCCGGCTACGCCGTGGCCGTGCCCGAGCGCTTCGGCCACTACCCGCCGCCCATGGACAGCCTCTCGC
CGTCATCGGAGACCGAAGCCGACGAGCAGTTCATCTGGATCCACGCCGTCTCCCTGGGGGAGACACGCGCGGCGGCCATC
CTGCTGAAGGAACTGCGCCCGCTGCTGCCCGGCATGCGCCTGCTGCTCACCCACGGCACGGCCACGGGCCGGGCCGAGGG
CGAAAAGCTGCTGCTGCCCGGCGACGTGCAGGTGTGGCAGCCCTGGGACACGCCCGGGGCCGTGCGGCGCTTTCTGCGGC
AGTTCCGCCCGTCTATCGGCATCCTCATGGAGACGGAGATCTGGCCCAACCTCGTCGCGGCCTGCCGCCGGCGGCGCATC
CCGCTGGTGCTGGCCAATGCGCGGCTCAACGAAAAGTCGCGCGCCGGCGCGCGCCGGCTGGGCTGGCTGTCGCGCCCGGC
CTACGCCGGCCTGTCGGCCGTGTGGGCGCAGACCGAAGACGACGCCGCCCGCCTACGCGACGTGGGCGCGCATGTGGCGG
GGGTCTTCGGCAACCTGAAGTTCGACGTGGTGCCGTCACCCGCCCTGCAGGCGCAGGGCCGTGCGTGGCGCGCCGCCAGC
GCCCGACCGGTGGTGCTGCTGGCCAGCAGCCGCGAGGGCGAAGAGGCCATGTGGCTGGAGGTTTTGAAGCAAAAAACGCC
TCTAGCGCCCGCCAGTCAATCGCCAACAGCTATTAATTCAGGAGTGAGCCAATCCGTGCAGTGGTTGGTGGTCCCGCGCC
ACCCCCAGCGTTTTGACGAGGTGCAGCGCCTGTGCGAGGCCGCCGGCCTGCGCGTGTCGCGGCGCAGCCAGTGGACCGCT
CAGCCGGACAGCGCCGATGTGTGGCTGGGCGACTCGCTGGGCGAGATGGCGCTGTATTACGGTCTGGCCCACGTGGCGCT
GCTGGGAGGCAGCTTTGCGCCGCTGGGCGGGCAGAACCTCATCGAGGCCGCGGCGGGCGGCTGCCCCGTGGTCATGGGCC
CGCACACCTTCAACTTTGCCGAGGCCGCGCGCCTGGCCATCGACGCCGGCGCCGCCCTGCGCGTGGCCGACATGGCCGAG
GGCGTGGCCGCCGCGGCCGCCCTGGCGCAGGACCCGCAGCGGCGGAGGGCGCTGTCCGAGCGCTGCGTGGCGTTCACCGA
GGAGCACCGCGGCGCGGCCCTGGACACGGCCCTGGCCGTCCTGCAGCGCCTGCGCGAGGCCGTGGACGACCGTCCCGACT
GA

Upstream 100 bases:

>100_bases
GCGCTGCAGCGCATCGAAGCCGCCATGCTGGCGCTGCTGGAACGCGTGAAGCCCGGCGCCCAGGTGGGCGGCGCCGCGCA
CTGAGGGCAGCGCGGGCGGC

Downstream 100 bases:

>100_bases
GCGGTCCGCCCGCGTTTCCTGCGATCACGACTGCAACCACGACTGCGAGGGCGCTGACACAGCGCAAGCCGCCGGCGTTG
CCTGTCTCGCCAAGCAACGC

Product: three-deoxy-d-manno-octulosonic-acid transferase domain-containing protein

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MHQLILWLYSLAVWLATPLLLRKLRRRALTEPGYAVAVPERFGHYPPPMDSLSPSSETEADEQFIWIHAVSLGETRAAAI
LLKELRPLLPGMRLLLTHGTATGRAEGEKLLLPGDVQVWQPWDTPGAVRRFLRQFRPSIGILMETEIWPNLVAACRRRRI
PLVLANARLNEKSRAGARRLGWLSRPAYAGLSAVWAQTEDDAARLRDVGAHVAGVFGNLKFDVVPSPALQAQGRAWRAAS
ARPVVLLASSREGEEAMWLEVLKQKTPLAPASQSPTAINSGVSQSVQWLVVPRHPQRFDEVQRLCEAAGLRVSRRSQWTA
QPDSADVWLGDSLGEMALYYGLAHVALLGGSFAPLGGQNLIEAAAGGCPVVMGPHTFNFAEAARLAIDAGAALRVADMAE
GVAAAAALAQDPQRRRALSERCVAFTEEHRGAALDTALAVLQRLREAVDDRPD

Sequences:

>Translated_453_residues
MHQLILWLYSLAVWLATPLLLRKLRRRALTEPGYAVAVPERFGHYPPPMDSLSPSSETEADEQFIWIHAVSLGETRAAAI
LLKELRPLLPGMRLLLTHGTATGRAEGEKLLLPGDVQVWQPWDTPGAVRRFLRQFRPSIGILMETEIWPNLVAACRRRRI
PLVLANARLNEKSRAGARRLGWLSRPAYAGLSAVWAQTEDDAARLRDVGAHVAGVFGNLKFDVVPSPALQAQGRAWRAAS
ARPVVLLASSREGEEAMWLEVLKQKTPLAPASQSPTAINSGVSQSVQWLVVPRHPQRFDEVQRLCEAAGLRVSRRSQWTA
QPDSADVWLGDSLGEMALYYGLAHVALLGGSFAPLGGQNLIEAAAGGCPVVMGPHTFNFAEAARLAIDAGAALRVADMAE
GVAAAAALAQDPQRRRALSERCVAFTEEHRGAALDTALAVLQRLREAVDDRPD
>Mature_453_residues
MHQLILWLYSLAVWLATPLLLRKLRRRALTEPGYAVAVPERFGHYPPPMDSLSPSSETEADEQFIWIHAVSLGETRAAAI
LLKELRPLLPGMRLLLTHGTATGRAEGEKLLLPGDVQVWQPWDTPGAVRRFLRQFRPSIGILMETEIWPNLVAACRRRRI
PLVLANARLNEKSRAGARRLGWLSRPAYAGLSAVWAQTEDDAARLRDVGAHVAGVFGNLKFDVVPSPALQAQGRAWRAAS
ARPVVLLASSREGEEAMWLEVLKQKTPLAPASQSPTAINSGVSQSVQWLVVPRHPQRFDEVQRLCEAAGLRVSRRSQWTA
QPDSADVWLGDSLGEMALYYGLAHVALLGGSFAPLGGQNLIEAAAGGCPVVMGPHTFNFAEAARLAIDAGAALRVADMAE
GVAAAAALAQDPQRRRALSERCVAFTEEHRGAALDTALAVLQRLREAVDDRPD

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=450, Percent_Identity=35.3333333333333, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 49367; Mature: 49367

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQLILWLYSLAVWLATPLLLRKLRRRALTEPGYAVAVPERFGHYPPPMDSLSPSSETEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCC
DEQFIWIHAVSLGETRAAAILLKELRPLLPGMRLLLTHGTATGRAEGEKLLLPGDVQVWQ
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEHCCCCCCCCCCCCEEEECCCCEEEC
PWDTPGAVRRFLRQFRPSIGILMETEIWPNLVAACRRRRIPLVLANARLNEKSRAGARRL
CCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
GWLSRPAYAGLSAVWAQTEDDAARLRDVGAHVAGVFGNLKFDVVPSPALQAQGRAWRAAS
CCCCCCHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCEECCC
ARPVVLLASSREGEEAMWLEVLKQKTPLAPASQSPTAINSGVSQSVQWLVVPRHPQRFDE
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCHHCCCEEEEECCCCHHHHH
VQRLCEAAGLRVSRRSQWTAQPDSADVWLGDSLGEMALYYGLAHVALLGGSFAPLGGQNL
HHHHHHHHCCEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
IEAAAGGCPVVMGPHTFNFAEAARLAIDAGAALRVADMAEGVAAAAALAQDPQRRRALSE
HHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHH
RCVAFTEEHRGAALDTALAVLQRLREAVDDRPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHQLILWLYSLAVWLATPLLLRKLRRRALTEPGYAVAVPERFGHYPPPMDSLSPSSETEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCC
DEQFIWIHAVSLGETRAAAILLKELRPLLPGMRLLLTHGTATGRAEGEKLLLPGDVQVWQ
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEHCCCCCCCCCCCCEEEECCCCEEEC
PWDTPGAVRRFLRQFRPSIGILMETEIWPNLVAACRRRRIPLVLANARLNEKSRAGARRL
CCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
GWLSRPAYAGLSAVWAQTEDDAARLRDVGAHVAGVFGNLKFDVVPSPALQAQGRAWRAAS
CCCCCCHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCEECCC
ARPVVLLASSREGEEAMWLEVLKQKTPLAPASQSPTAINSGVSQSVQWLVVPRHPQRFDE
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCHHCCCEEEEECCCCHHHHH
VQRLCEAAGLRVSRRSQWTAQPDSADVWLGDSLGEMALYYGLAHVALLGGSFAPLGGQNL
HHHHHHHHCCEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
IEAAAGGCPVVMGPHTFNFAEAARLAIDAGAALRVADMAEGVAAAAALAQDPQRRRALSE
HHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHH
RCVAFTEEHRGAALDTALAVLQRLREAVDDRPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]