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The map label for this gene is gudD [H]

Identifier: 222110989

GI number: 222110989

Start: 1912200

End: 1913507

Strand: Direct

Name: gudD [H]

Synonym: Dtpsy_1796

Alternate gene names: 222110989

Gene position: 1912200-1913507 (Clockwise)

Preceding gene: 222110979

Following gene: 222110990

Centisome position: 50.37

GC content: 66.97

Gene sequence:

>1308_bases
ATGAGCACCTCCCACGCGATCCGCCATATCCGCATCACACCCATTGCCTTCCGCGATCCGCCGCTGCTCAATGCGGCCGG
CATCCACGAGCCCTGGGCCCTGCGCTCCATCATCGAGATCGAGACCGCCTCGGGTCTGGTGGGCATCAACGAGAGCTATG
GCGACCAGCCCATGCTGGACGCTTTGGCCAAGGCGGCGCCCGCCCTGGTGGGTCTATCCCCCTGGGCGCTCAACGCAATG
GAGCAGCGCGTGGCGGCCCTGGTCGCACCGCCCGCACAAACGGCCTCGGAATTCCTGGGGCAGCAGGTGTCGCTGGCACC
GGGCACGCATGTATCCAAGACCGTGGCCAAGGTCATCAGCGCGTTTGAAGTGGCGATGATGGACCTGCAAGGCCAGTTGG
CCCAGGCCCCGGTCGTGGATCTGCTGGCCGGCGCGGCGCGCGACAGCGTTCCCTTCTCGGCCTACCTGTTCTACAAATAC
GCCGAACACATCGACCAGCCCTATACGCCGGATGCCTGGGGCGAGGCACTCACGCCCGCGCAACTCGTCGCCCAGGCGCG
GCGCATGATCGCCCAGTACGGCTTCCAAAGCATCAAGCTCAAGGCCGGCGCCTTGCCGCCCGACCAGGAGGCGGCCGGCA
TCCTCGCGCTGGCCCAGGCCTTTCCGGGTATGCCGTTGCGCATCGACCCCAACGCCAACTGGACGGTACAGACCAGCCTG
AAGGTGGTGCAACAACTGCGCGGCGTGCTGGAGTATTACGAAGACCCGGCCCCTGGCCTAGAGGGCATGGCTGCCGTGGC
GCGCGCGATGGAACGCGAAGGCGATGTGCCGCTGGCCACCAACATGGTGGTCACCGACTTTGCCGAATTCCGCCGCAACG
TCGACCTGGGCTGCCCCGTGAAGATCGTGCTCAGCGACCACCACTACTGGGGCGGTCTGCGTGCCACGCAACGGCTGGCC
ACGCTGTGCAAGACCTTCGACCTGGGTCTGTCCATGCACAGCAACTCCCACCTGGGCATCAGCCTCGTCGCCATGACGCA
CCTGTGTGCCAGCGTGCCGCAGCTCACCTATGCCTGCGACACGCACTACCCGTGGCAGGACGAGGAGGTCGTCGAAGGCG
GCCGCCTGCAATTCGAGCAGGGCAGCCTGCGCGTGCCTACGACGGCGGGCCTGGGCGTCACGCTGGATCGCGAGGCGCTG
GCCCGCCTGCACGACAACTACCTGCACTGCGGCATCCGTAACCGCGACGACCTCGGGCAGATGCGCAAGTACGACCCCGG
CTTCACCGGCCGCCAGCCACGTTTCTGA

Upstream 100 bases:

>100_bases
TGGATGTGATCATAATTCGTTGCACGTTATAAGACAACATATGTCTTAGCCGAGCCGCACCATGAAATGCCGGCACGGAT
GCCCCTCTCAACGCACGAAG

Downstream 100 bases:

>100_bases
GTCCTTTCCATCCAACCAGGAGACATCCCCCATGCAACGCCGCACCTTGATCAAAAGCTCCCTCGCCGCCGCCAGCGCCT
TGCTGCTGGGCAGCGCATTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSTSHAIRHIRITPIAFRDPPLLNAAGIHEPWALRSIIEIETASGLVGINESYGDQPMLDALAKAAPALVGLSPWALNAM
EQRVAALVAPPAQTASEFLGQQVSLAPGTHVSKTVAKVISAFEVAMMDLQGQLAQAPVVDLLAGAARDSVPFSAYLFYKY
AEHIDQPYTPDAWGEALTPAQLVAQARRMIAQYGFQSIKLKAGALPPDQEAAGILALAQAFPGMPLRIDPNANWTVQTSL
KVVQQLRGVLEYYEDPAPGLEGMAAVARAMEREGDVPLATNMVVTDFAEFRRNVDLGCPVKIVLSDHHYWGGLRATQRLA
TLCKTFDLGLSMHSNSHLGISLVAMTHLCASVPQLTYACDTHYPWQDEEVVEGGRLQFEQGSLRVPTTAGLGVTLDREAL
ARLHDNYLHCGIRNRDDLGQMRKYDPGFTGRQPRF

Sequences:

>Translated_435_residues
MSTSHAIRHIRITPIAFRDPPLLNAAGIHEPWALRSIIEIETASGLVGINESYGDQPMLDALAKAAPALVGLSPWALNAM
EQRVAALVAPPAQTASEFLGQQVSLAPGTHVSKTVAKVISAFEVAMMDLQGQLAQAPVVDLLAGAARDSVPFSAYLFYKY
AEHIDQPYTPDAWGEALTPAQLVAQARRMIAQYGFQSIKLKAGALPPDQEAAGILALAQAFPGMPLRIDPNANWTVQTSL
KVVQQLRGVLEYYEDPAPGLEGMAAVARAMEREGDVPLATNMVVTDFAEFRRNVDLGCPVKIVLSDHHYWGGLRATQRLA
TLCKTFDLGLSMHSNSHLGISLVAMTHLCASVPQLTYACDTHYPWQDEEVVEGGRLQFEQGSLRVPTTAGLGVTLDREAL
ARLHDNYLHCGIRNRDDLGQMRKYDPGFTGRQPRF
>Mature_434_residues
STSHAIRHIRITPIAFRDPPLLNAAGIHEPWALRSIIEIETASGLVGINESYGDQPMLDALAKAAPALVGLSPWALNAME
QRVAALVAPPAQTASEFLGQQVSLAPGTHVSKTVAKVISAFEVAMMDLQGQLAQAPVVDLLAGAARDSVPFSAYLFYKYA
EHIDQPYTPDAWGEALTPAQLVAQARRMIAQYGFQSIKLKAGALPPDQEAAGILALAQAFPGMPLRIDPNANWTVQTSLK
VVQQLRGVLEYYEDPAPGLEGMAAVARAMEREGDVPLATNMVVTDFAEFRRNVDLGCPVKIVLSDHHYWGGLRATQRLAT
LCKTFDLGLSMHSNSHLGISLVAMTHLCASVPQLTYACDTHYPWQDEEVVEGGRLQFEQGSLRVPTTAGLGVTLDREALA
RLHDNYLHCGIRNRDDLGQMRKYDPGFTGRQPRF

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=447, Percent_Identity=31.5436241610738, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789151, Length=447, Percent_Identity=31.0961968680089, Blast_Score=188, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 47324; Mature: 47193

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSHAIRHIRITPIAFRDPPLLNAAGIHEPWALRSIIEIETASGLVGINESYGDQPMLD
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHEECCCCEEECCCCCCCCHHHH
ALAKAAPALVGLSPWALNAMEQRVAALVAPPAQTASEFLGQQVSLAPGTHVSKTVAKVIS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCHHHHHHHHHHH
AFEVAMMDLQGQLAQAPVVDLLAGAARDSVPFSAYLFYKYAEHIDQPYTPDAWGEALTPA
HHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
QLVAQARRMIAQYGFQSIKLKAGALPPDQEAAGILALAQAFPGMPLRIDPNANWTVQTSL
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHH
KVVQQLRGVLEYYEDPAPGLEGMAAVARAMEREGDVPLATNMVVTDFAEFRRNVDLGCPV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCCCCE
KIVLSDHHYWGGLRATQRLATLCKTFDLGLSMHSNSHLGISLVAMTHLCASVPQLTYACD
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEC
THYPWQDEEVVEGGRLQFEQGSLRVPTTAGLGVTLDREALARLHDNYLHCGIRNRDDLGQ
CCCCCCCHHHHCCCCEEEECCCEECCCCCCCCEEECHHHHHHHHCCCEECCCCCCHHHHH
MRKYDPGFTGRQPRF
HHHCCCCCCCCCCCC
>Mature Secondary Structure 
STSHAIRHIRITPIAFRDPPLLNAAGIHEPWALRSIIEIETASGLVGINESYGDQPMLD
CCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHEECCCCEEECCCCCCCCHHHH
ALAKAAPALVGLSPWALNAMEQRVAALVAPPAQTASEFLGQQVSLAPGTHVSKTVAKVIS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCHHHHHHHHHHH
AFEVAMMDLQGQLAQAPVVDLLAGAARDSVPFSAYLFYKYAEHIDQPYTPDAWGEALTPA
HHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
QLVAQARRMIAQYGFQSIKLKAGALPPDQEAAGILALAQAFPGMPLRIDPNANWTVQTSL
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHH
KVVQQLRGVLEYYEDPAPGLEGMAAVARAMEREGDVPLATNMVVTDFAEFRRNVDLGCPV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCCCCE
KIVLSDHHYWGGLRATQRLATLCKTFDLGLSMHSNSHLGISLVAMTHLCASVPQLTYACD
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEC
THYPWQDEEVVEGGRLQFEQGSLRVPTTAGLGVTLDREALARLHDNYLHCGIRNRDDLGQ
CCCCCCCHHHHCCCCEEEECCCEECCCCCCCCEEECHHHHHHHHCCCEECCCCCCHHHHH
MRKYDPGFTGRQPRF
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]