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The map label for this gene is eptA [H]

Identifier: 222110949

GI number: 222110949

Start: 1867000

End: 1868652

Strand: Direct

Name: eptA [H]

Synonym: Dtpsy_1756

Alternate gene names: 222110949

Gene position: 1867000-1868652 (Clockwise)

Preceding gene: 222110948

Following gene: 222110953

Centisome position: 49.18

GC content: 68.24

Gene sequence:

>1653_bases
ATGACCACCCCGCTGTCGGCGCAGCAGGTGGTGGTGCGCCTGTCGCTGTACTTGGTACTGGCGGCCAACTGGCCGCTGTG
GCTGCAGATCACGCGCATTGGCGGCGCGCCCAGCCTGTACCTGCGTTCAGCGGCTGCCTTGGCCGTGCTGACGGCGTGCG
GCACGGTGGCCATTTTGGCGTTCACCGCCTGGACGCGCGGCATGAAGCTGCTGTGGTGGATGGTGGTCATGGTGGCCGCG
CTGTCGCAGTACTACATGCTGACCTACAGCGTGGTCATGGACCCCAGCATGGCCGCCAACGTGTTGCAGACGGATGCGCG
CGAGGTGGCCGACCTGCTGAGCCTGCGCATGCTGGCTGCGGTGCTGGCCGTCGCGGCGCTTCCCTCCTGGTGGTTGCTGC
GTGTGCGTATTCCCGCCATGCGGCCGCTGCGCCAGGTGGGCCGCAACCTGGCGCTGCTGGTGCTGGCGCTGGGCCTGGCC
GCCGGCACGGTGGCGGCCATGTCGCGGGACCTGGCGCCGCTGATGCGCAACCACCCGCACCTGCGCTACCTGATGAACCC
CTTGGCCAGTCTGTACTCCACCTCCGTGTCGGCCCTGCGACCGGTGTTCGCGCGCAGCCGCACGCTGGTGCCGATGACCC
AGGGCGCCGCGCTGGGCGCCAGCTATGCGGCCGGGGCGCGGCCCACGCTGTTCGTGCTGGTGGTGGGCGAGACGGCGCGC
GCGGACCATTTCGGCATCAACGGCTATGGCCGCGACACCACGCCGGAACTGACCAAACGCAACGTGATGAGCTGGCGCGA
AGTGCGCTCGTGCGGCACCAGCACGCTGGCGTCGCTGCCTTGCATGTTCTCGCCACTGGGCAAGAGTGCATTCGAGTCGC
GTGACAACGACTACGAGAACCTGCTGGACGTGGCGCAGGCCGCGGGTATGGGCGTGCTGTGGCTGGACAACCAGTCCGGC
TGCAAGGGCGTGTGCGACCGCGTGCCGCACGCATCGGCCGCCGAAGGCCTGGATGCCGAGGCGCGCAAGCAGTTGTGCGA
TGGCGGAGAATGCCGCGACGAGGCCATGCTGCACAACCTGGATGCGCGCCTGGCCGCCTTGCCTGCGGCGCAGCGCGAGC
GCGGCGTGCTGCTGGTGCTGCACCAGATGGGCAGCCATGGGCCGGCCTACTACAAGCGTTCGGCCGATGCAGGCAAGCGC
TTCATACCCGAATGCCGCACGGAAGTGCTGGCCGACTGCACCCACGGCGAACTGGTGAACGCCTACGACAACTCCATCGC
CGCAACGGACGCCTTCCTGGGCAAGACCATCGACTGGCTGCGTGGCCAGAGCGCCAAGTACGACACGGCTCTGCTGTACC
TGAGCGACCATGGCGAGTCGCTGGGCGAGTACGGGTTGTTCCTGCACGGCATGCCCTACGGCGTGGCGCCCGACATGCAA
AAGCACGTGCCCATGGTGGCGTGGATGGACACCTCCTTCATGCGCCGTGACGGGTTAGCGGCCGACTGCCTGCGCGCCAG
CGGCGATGCGCCGTTGACGCACGACAACCTCTACCACACCGTGCTGGGCCTGCTGGACGTGCAGTCGCCTTCCTACCAGC
GCCCGCTGGATGCCTTCAGCGGCTGCCGGAGCACCGTGTTGGCATCCAAATAA

Upstream 100 bases:

>100_bases
CATTGCCTCTGCCTCCTCGGCCTCCGTGCCGCTTGTGTCATCTTCCAGCCCTGCCGACGAAGGCCTCTTGCGTGGTGCGT
TGCGCCGGCTGGATGGCTGG

Downstream 100 bases:

>100_bases
AAACAGCCGCCAACGCTTATCTATAAAGCGCGAGCAGCTATTTTTTTAAGAGCATCTTGGCGCTCAGGCCTTCTTCTCGC
GCGGCAGGCGGCCCATCAGG

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTTPLSAQQVVVRLSLYLVLAANWPLWLQITRIGGAPSLYLRSAAALAVLTACGTVAILAFTAWTRGMKLLWWMVVMVAA
LSQYYMLTYSVVMDPSMAANVLQTDAREVADLLSLRMLAAVLAVAALPSWWLLRVRIPAMRPLRQVGRNLALLVLALGLA
AGTVAAMSRDLAPLMRNHPHLRYLMNPLASLYSTSVSALRPVFARSRTLVPMTQGAALGASYAAGARPTLFVLVVGETAR
ADHFGINGYGRDTTPELTKRNVMSWREVRSCGTSTLASLPCMFSPLGKSAFESRDNDYENLLDVAQAAGMGVLWLDNQSG
CKGVCDRVPHASAAEGLDAEARKQLCDGGECRDEAMLHNLDARLAALPAAQRERGVLLVLHQMGSHGPAYYKRSADAGKR
FIPECRTEVLADCTHGELVNAYDNSIAATDAFLGKTIDWLRGQSAKYDTALLYLSDHGESLGEYGLFLHGMPYGVAPDMQ
KHVPMVAWMDTSFMRRDGLAADCLRASGDAPLTHDNLYHTVLGLLDVQSPSYQRPLDAFSGCRSTVLASK

Sequences:

>Translated_550_residues
MTTPLSAQQVVVRLSLYLVLAANWPLWLQITRIGGAPSLYLRSAAALAVLTACGTVAILAFTAWTRGMKLLWWMVVMVAA
LSQYYMLTYSVVMDPSMAANVLQTDAREVADLLSLRMLAAVLAVAALPSWWLLRVRIPAMRPLRQVGRNLALLVLALGLA
AGTVAAMSRDLAPLMRNHPHLRYLMNPLASLYSTSVSALRPVFARSRTLVPMTQGAALGASYAAGARPTLFVLVVGETAR
ADHFGINGYGRDTTPELTKRNVMSWREVRSCGTSTLASLPCMFSPLGKSAFESRDNDYENLLDVAQAAGMGVLWLDNQSG
CKGVCDRVPHASAAEGLDAEARKQLCDGGECRDEAMLHNLDARLAALPAAQRERGVLLVLHQMGSHGPAYYKRSADAGKR
FIPECRTEVLADCTHGELVNAYDNSIAATDAFLGKTIDWLRGQSAKYDTALLYLSDHGESLGEYGLFLHGMPYGVAPDMQ
KHVPMVAWMDTSFMRRDGLAADCLRASGDAPLTHDNLYHTVLGLLDVQSPSYQRPLDAFSGCRSTVLASK
>Mature_549_residues
TTPLSAQQVVVRLSLYLVLAANWPLWLQITRIGGAPSLYLRSAAALAVLTACGTVAILAFTAWTRGMKLLWWMVVMVAAL
SQYYMLTYSVVMDPSMAANVLQTDAREVADLLSLRMLAAVLAVAALPSWWLLRVRIPAMRPLRQVGRNLALLVLALGLAA
GTVAAMSRDLAPLMRNHPHLRYLMNPLASLYSTSVSALRPVFARSRTLVPMTQGAALGASYAAGARPTLFVLVVGETARA
DHFGINGYGRDTTPELTKRNVMSWREVRSCGTSTLASLPCMFSPLGKSAFESRDNDYENLLDVAQAAGMGVLWLDNQSGC
KGVCDRVPHASAAEGLDAEARKQLCDGGECRDEAMLHNLDARLAALPAAQRERGVLLVLHQMGSHGPAYYKRSADAGKRF
IPECRTEVLADCTHGELVNAYDNSIAATDAFLGKTIDWLRGQSAKYDTALLYLSDHGESLGEYGLFLHGMPYGVAPDMQK
HVPMVAWMDTSFMRRDGLAADCLRASGDAPLTHDNLYHTVLGLLDVQSPSYQRPLDAFSGCRSTVLASK

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=479, Percent_Identity=37.5782881002088, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI87082286, Length=489, Percent_Identity=29.038854805726, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI87082223, Length=429, Percent_Identity=25.8741258741259, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787035, Length=244, Percent_Identity=27.0491803278689, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59691; Mature: 59560

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLSAQQVVVRLSLYLVLAANWPLWLQITRIGGAPSLYLRSAAALAVLTACGTVAILA
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTAWTRGMKLLWWMVVMVAALSQYYMLTYSVVMDPSMAANVLQTDAREVADLLSLRMLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLAVAALPSWWLLRVRIPAMRPLRQVGRNLALLVLALGLAAGTVAAMSRDLAPLMRNHPH
HHHHHHCCCHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LRYLMNPLASLYSTSVSALRPVFARSRTLVPMTQGAALGASYAAGARPTLFVLVVGETAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCHHCCCCCCEEEEEEECCCCC
ADHFGINGYGRDTTPELTKRNVMSWREVRSCGTSTLASLPCMFSPLGKSAFESRDNDYEN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCCCHHHHHCCCCCHHH
LLDVAQAAGMGVLWLDNQSGCKGVCDRVPHASAAEGLDAEARKQLCDGGECRDEAMLHNL
HHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCHHCCCCHHHHHHHCCCCCCHHHHHHHHH
DARLAALPAAQRERGVLLVLHQMGSHGPAYYKRSADAGKRFIPECRTEVLADCTHGELVN
HHHHHHCCCHHHCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHH
AYDNSIAATDAFLGKTIDWLRGQSAKYDTALLYLSDHGESLGEYGLFLHGMPYGVAPDMQ
HHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHCCEEECCCCCCCCCHH
KHVPMVAWMDTSFMRRDGLAADCLRASGDAPLTHDNLYHTVLGLLDVQSPSYQRPLDAFS
HCCCEEEECCHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
GCRSTVLASK
HHHHHHHCCC
>Mature Secondary Structure 
TTPLSAQQVVVRLSLYLVLAANWPLWLQITRIGGAPSLYLRSAAALAVLTACGTVAILA
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTAWTRGMKLLWWMVVMVAALSQYYMLTYSVVMDPSMAANVLQTDAREVADLLSLRMLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLAVAALPSWWLLRVRIPAMRPLRQVGRNLALLVLALGLAAGTVAAMSRDLAPLMRNHPH
HHHHHHCCCHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LRYLMNPLASLYSTSVSALRPVFARSRTLVPMTQGAALGASYAAGARPTLFVLVVGETAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCHHCCCCCCEEEEEEECCCCC
ADHFGINGYGRDTTPELTKRNVMSWREVRSCGTSTLASLPCMFSPLGKSAFESRDNDYEN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCCCHHHHHCCCCCHHH
LLDVAQAAGMGVLWLDNQSGCKGVCDRVPHASAAEGLDAEARKQLCDGGECRDEAMLHNL
HHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCHHCCCCHHHHHHHCCCCCCHHHHHHHHH
DARLAALPAAQRERGVLLVLHQMGSHGPAYYKRSADAGKRFIPECRTEVLADCTHGELVN
HHHHHHCCCHHHCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHH
AYDNSIAATDAFLGKTIDWLRGQSAKYDTALLYLSDHGESLGEYGLFLHGMPYGVAPDMQ
HHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHCCEEECCCCCCCCCHH
KHVPMVAWMDTSFMRRDGLAADCLRASGDAPLTHDNLYHTVLGLLDVQSPSYQRPLDAFS
HCCCEEEECCHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
GCRSTVLASK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]