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The map label for this gene is phoB [H]

Identifier: 222110688

GI number: 222110688

Start: 1564866

End: 1565573

Strand: Direct

Name: phoB [H]

Synonym: Dtpsy_1491

Alternate gene names: 222110688

Gene position: 1564866-1565573 (Clockwise)

Preceding gene: 222110687

Following gene: 222110689

Centisome position: 41.22

GC content: 64.83

Gene sequence:

>708_bases
ATGAAGAAGCTTCCCCGGGTACTCATCGTCGAGGACGAGTCCCCCATCGCAGAATTGGTGGCCGTGAACCTGCGCCACAA
CGGCTTTCAGCCCATCTGGGCGGAAGACGGCGAGTCGGCGCAGCGCGAGCTGGACGCGGTGCTGCCCGATGTCATCTTGC
TGGACTGGATGCTGCCGGGACAAAGTGGCCTGCAACTGGCACGCAAGTGGCGTGCCGACAGCCGCACCAAACAGATTCCG
ATCCTCATGCTGACGGCGCGCGGTGACGAGCCCGACAAGGTGGCCGGGCTGGATGCCGGTGCCGACGACTACATCACCAA
GCCGTTTTCCACGCAGGAACTGCTGGCGCGCATCCGCGCCGTGCTGCGCCGGCGTGCGCCCGAGCAGGTCAGCGACAGTG
TCTCTATTGGTGAACTGGTGCTGGATGCGGCCACGTATCGCGTCAGCTACCAGGGCCAGCAGCTCAAGGTCGGGCCGACA
GAGTTCAAGCTGCTGCACTACCTGATGAAGCACCCGGAGCGCGTGCACAGCCGCGCGCAACTGCTCGACAAGATTTGGGG
GGACCACGTCTTCATCGAGGAGCGTACGGTGGATGTGCACGTGAAACGCTTGCGTGAAGCGCTGGGGGTAGGCGGCGCCA
TGGTGGAGACCGTGCGTGGAGCGGGATACCGGCTCACCGCGCAGCCGCAGACACTCATGCGGGCGTGA

Upstream 100 bases:

>100_bases
GTGGCCGAGCTGATCATCTATCTCGTCAAGGGCAAGGACGTGCGCCACACGGCGCTGGACGAGATCGAGTCGGCCGTGGC
CTGAGAAAGGCGGTGAACCG

Downstream 100 bases:

>100_bases
TGGCGGGTGCCCGACGCCTCCGTGTTTTTTCAGGCGCCCGGAGATGATGCGGGCGCTTTTTTGTTGTTGACCTGAGCCCT
GCGGCCATGCTCCTGCGCTT

Product: winged helix family two component heavy metal response transcriptional regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKKLPRVLIVEDESPIAELVAVNLRHNGFQPIWAEDGESAQRELDAVLPDVILLDWMLPGQSGLQLARKWRADSRTKQIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPEQVSDSVSIGELVLDAATYRVSYQGQQLKVGPT
EFKLLHYLMKHPERVHSRAQLLDKIWGDHVFIEERTVDVHVKRLREALGVGGAMVETVRGAGYRLTAQPQTLMRA

Sequences:

>Translated_235_residues
MKKLPRVLIVEDESPIAELVAVNLRHNGFQPIWAEDGESAQRELDAVLPDVILLDWMLPGQSGLQLARKWRADSRTKQIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPEQVSDSVSIGELVLDAATYRVSYQGQQLKVGPT
EFKLLHYLMKHPERVHSRAQLLDKIWGDHVFIEERTVDVHVKRLREALGVGGAMVETVRGAGYRLTAQPQTLMRA
>Mature_235_residues
MKKLPRVLIVEDESPIAELVAVNLRHNGFQPIWAEDGESAQRELDAVLPDVILLDWMLPGQSGLQLARKWRADSRTKQIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPEQVSDSVSIGELVLDAATYRVSYQGQQLKVGPT
EFKLLHYLMKHPERVHSRAQLLDKIWGDHVFIEERTVDVHVKRLREALGVGGAMVETVRGAGYRLTAQPQTLMRA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=55.5555555555556, Blast_Score=263, Evalue=9e-72,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=40.174672489083, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=35.7758620689655, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=39.0134529147982, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=35.1351351351351, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=35.8974358974359, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=35, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=34.5454545454545, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1790552, Length=227, Percent_Identity=36.1233480176211, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=34.9344978165939, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=32.9059829059829, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082012, Length=227, Percent_Identity=34.3612334801762, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=29.3859649122807, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=31.6666666666667, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=30.7017543859649, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26401; Mature: 26401

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLPRVLIVEDESPIAELVAVNLRHNGFQPIWAEDGESAQRELDAVLPDVILLDWMLPG
CCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
QSGLQLARKWRADSRTKQIPILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRA
CHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
VLRRRAPEQVSDSVSIGELVLDAATYRVSYQGQQLKVGPTEFKLLHYLMKHPERVHSRAQ
HHHHCCCHHHHCCCHHHHHHHHHHHEEEEECCCEEEECHHHHHHHHHHHHCHHHHHHHHH
LLDKIWGDHVFIEERTVDVHVKRLREALGVGGAMVETVRGAGYRLTAQPQTLMRA
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCHHHHCC
>Mature Secondary Structure
MKKLPRVLIVEDESPIAELVAVNLRHNGFQPIWAEDGESAQRELDAVLPDVILLDWMLPG
CCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
QSGLQLARKWRADSRTKQIPILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRA
CHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
VLRRRAPEQVSDSVSIGELVLDAATYRVSYQGQQLKVGPTEFKLLHYLMKHPERVHSRAQ
HHHHCCCHHHHCCCHHHHHHHHHHHEEEEECCCEEEECHHHHHHHHHHHHCHHHHHHHHH
LLDKIWGDHVFIEERTVDVHVKRLREALGVGGAMVETVRGAGYRLTAQPQTLMRA
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]