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The map label for this gene is cysL [H]

Identifier: 222110620

GI number: 222110620

Start: 1489984

End: 1490871

Strand: Direct

Name: cysL [H]

Synonym: Dtpsy_1421

Alternate gene names: 222110620

Gene position: 1489984-1490871 (Clockwise)

Preceding gene: 222110615

Following gene: 222110624

Centisome position: 39.25

GC content: 58.0

Gene sequence:

>888_bases
ATGTCTGGACTCAGCCTCCTCGCAGCCTTGAAGGCCTTTGACGCAACGGCCCGAGCCGGCAGCATGACCGCTGCCGCGAG
AGTGCTCGCTATTCAACAACCGACTGTTTCTTCACACATACAGCGCCTGGAGGTCGACTTCGGCGTGGAGCTTTTTCATC
GGCGCGGGCGGCGGCTTGAACTCACGTCCTTTGGACGCACTCTGTTGGACTACACCCGGCGTACCTTCAGTGGCGAGGAA
GATGCGCACGCACTGCTCGCTGCTGCGAAAAATCAATACGTAGGGCGACTGGTCATCCATGCCATCGGGCCGCACAACGT
GCTGCCCGTGTTGAAGCTGTTCATGAAACAGTTCCCCCAGGTCAACGTGGCTGTTGGCGTAGGCGACTCGCGAACCATCA
CTGAAAAGATTCTTGACTACCAGGGAGACGTGGGCATGGTGCTCAATCACGTCGCTCACCCAGATCTATTTGGCGCGGCT
TACCGTACCCAGCGATTGGTGATATTCGGCAACACCCAACACCCACTCGCGCAACGGGCCACTCTCAAACTGCGTGATCT
GCAGGGTCAGAGGTTCGTCATCCGAGAGGAAGGATCGACGACCAGACGCGTGTTTGAACAAGAACTAAAGGAGCGAGGCG
TCGAGGTGCAGGTGGCTCTGGAGATGGGCAGCCGCGAGTCCGTTCGAGAGGCGGTGGCGGAGGGGCTGGGCCTAGGCGTC
GTGGCGGAAACAGCCTACAGGCCGGATCCTCGCCTTGTTAAGTTAACTATTTCAGACACCAGCATGGCGACACAGGTGCA
CTTCATCTGTAGAAAGGAGCGGCATCAAGCTCCTCTGATCTCGACCTTCCTGGGTTTGGCGCATAGCGTAAGTCAGAAAA
TTGCCTAG

Upstream 100 bases:

>100_bases
AAACAGCTTCAGTGGAAGCACAGCTCAAGTCTGGGTGACCAAGGTGTCATGAATATGAAACTTTCATCCCTAAAAATGAG
GGCACCCATAGATCACATCA

Downstream 100 bases:

>100_bases
GTATGGAACTTGGATTCCATGCAGCCGTGGGGCATGGGACCGAGGGGCAACTGTTCGCCTGTAGGCCGACTTAAGTTAAG
GGTTGTAGGACGCCCGTAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSGLSLLAALKAFDATARAGSMTAAARVLAIQQPTVSSHIQRLEVDFGVELFHRRGRRLELTSFGRTLLDYTRRTFSGEE
DAHALLAAAKNQYVGRLVIHAIGPHNVLPVLKLFMKQFPQVNVAVGVGDSRTITEKILDYQGDVGMVLNHVAHPDLFGAA
YRTQRLVIFGNTQHPLAQRATLKLRDLQGQRFVIREEGSTTRRVFEQELKERGVEVQVALEMGSRESVREAVAEGLGLGV
VAETAYRPDPRLVKLTISDTSMATQVHFICRKERHQAPLISTFLGLAHSVSQKIA

Sequences:

>Translated_295_residues
MSGLSLLAALKAFDATARAGSMTAAARVLAIQQPTVSSHIQRLEVDFGVELFHRRGRRLELTSFGRTLLDYTRRTFSGEE
DAHALLAAAKNQYVGRLVIHAIGPHNVLPVLKLFMKQFPQVNVAVGVGDSRTITEKILDYQGDVGMVLNHVAHPDLFGAA
YRTQRLVIFGNTQHPLAQRATLKLRDLQGQRFVIREEGSTTRRVFEQELKERGVEVQVALEMGSRESVREAVAEGLGLGV
VAETAYRPDPRLVKLTISDTSMATQVHFICRKERHQAPLISTFLGLAHSVSQKIA
>Mature_294_residues
SGLSLLAALKAFDATARAGSMTAAARVLAIQQPTVSSHIQRLEVDFGVELFHRRGRRLELTSFGRTLLDYTRRTFSGEED
AHALLAAAKNQYVGRLVIHAIGPHNVLPVLKLFMKQFPQVNVAVGVGDSRTITEKILDYQGDVGMVLNHVAHPDLFGAAY
RTQRLVIFGNTQHPLAQRATLKLRDLQGQRFVIREEGSTTRRVFEQELKERGVEVQVALEMGSRESVREAVAEGLGLGVV
AETAYRPDPRLVKLTISDTSMATQVHFICRKERHQAPLISTFLGLAHSVSQKIA

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=271, Percent_Identity=29.1512915129151, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693105, Length=286, Percent_Identity=24.1258741258741, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788296, Length=227, Percent_Identity=26.431718061674, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=26.8398268398268, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082132, Length=231, Percent_Identity=28.1385281385281, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787806, Length=240, Percent_Identity=22.5, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787530, Length=247, Percent_Identity=22.2672064777328, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=243, Percent_Identity=22.6337448559671, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=304, Percent_Identity=22.3684210526316, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32612; Mature: 32481

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLSLLAALKAFDATARAGSMTAAARVLAIQQPTVSSHIQRLEVDFGVELFHRRGRRLE
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCEEE
LTSFGRTLLDYTRRTFSGEEDAHALLAAAKNQYVGRLVIHAIGPHNVLPVLKLFMKQFPQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCC
VNVAVGVGDSRTITEKILDYQGDVGMVLNHVAHPDLFGAAYRTQRLVIFGNTQHPLAQRA
EEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCCHHHHH
TLKLRDLQGQRFVIREEGSTTRRVFEQELKERGVEVQVALEMGSRESVREAVAEGLGLGV
HHHEEECCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCCE
VAETAYRPDPRLVKLTISDTSMATQVHFICRKERHQAPLISTFLGLAHSVSQKIA
EECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGLSLLAALKAFDATARAGSMTAAARVLAIQQPTVSSHIQRLEVDFGVELFHRRGRRLE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCEEE
LTSFGRTLLDYTRRTFSGEEDAHALLAAAKNQYVGRLVIHAIGPHNVLPVLKLFMKQFPQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCC
VNVAVGVGDSRTITEKILDYQGDVGMVLNHVAHPDLFGAAYRTQRLVIFGNTQHPLAQRA
EEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCCHHHHH
TLKLRDLQGQRFVIREEGSTTRRVFEQELKERGVEVQVALEMGSRESVREAVAEGLGLGV
HHHEEECCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCCE
VAETAYRPDPRLVKLTISDTSMATQVHFICRKERHQAPLISTFLGLAHSVSQKIA
EECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]