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The map label for this gene is gcvA [H]

Identifier: 222110541

GI number: 222110541

Start: 1406580

End: 1407497

Strand: Direct

Name: gcvA [H]

Synonym: Dtpsy_1341

Alternate gene names: 222110541

Gene position: 1406580-1407497 (Clockwise)

Preceding gene: 222110539

Following gene: 222110547

Centisome position: 37.05

GC content: 71.57

Gene sequence:

>918_bases
ATGTTTCGCAAACTCTTCCTGCCGCCCGTGGCCGACCTGCTCGCCTTTGAGGCCGCCGCGCGCCACGCCAGCATCTCACG
TGCGGCGGAGGAGCTGCACCTCACGCAAAGCGCCGTCTCGCGCCAGGTGCGCCAACTGGAGGCACAGCTCGGTCAGGCCC
TGTTCACCCGGGTGCGCCAGCGCGTGGTGTTGACGGATGCGGGGCGCCTGTACGCCGCCGACGTACGCGCCGTGCTGCAG
CAGCTCGGTGCCGCCACGCAGAAGGCCATGGCCCTGGCCGATGGCGGCGGACTCCTGCACCTGGCGGTGCTGCCCACGTT
GGGCGCGCGCTGGCTGGTGCCGCGCCTGCCTGCGTTTGCCGCGCTGCACCCGGAGCTGACGGTGAATCTTTCTGCCCGTG
GCGAGCCTTTTGACTTCGCCCAGGAGCCGTTCGATGCCGCTATCCACTACGGTGCGCCCCACTGGGCCGGCGCCGTGTGC
GACTACCTGATGCACGAAGAAGTGGTGCCGGTGTGCAGCCCCGCGCTGCGCACCCGCCACCGCATCGAGACCCCTGCCGA
CGTGGCCGGCCTGACCCTGCTGCACCAGACCACGCGCCCCGTGCTGTGGGCCGACTGGTTCGAGCAAGAGGGCGTGCCCG
CGCCCCAGGCCCTGCGCGGCCCGCGTTTCGAGCAGTTCGCCATGATCGCCCAGGCCGCCATCTCCGGCCTGGGCGTGGCC
CTGCTGCCGCGGTTTCTGATGGAGGAGGAATTGGCGAGCGGCGCGCTGGTGGAGCTGCCCGGTGGCGTGCTGACCAGCAG
CGATGCGTACTACCTCGTGGTGCCGCTGGCGCGGGCAGAGTCGGCGGGGGTGAAGGCCTTCAGGGCGTGGCTGCGGGAGG
TGGCGGGCCAGCGGGAGCTGGGCCGCGTTGGCGGATGA

Upstream 100 bases:

>100_bases
AACCCTGCCTGCCGCCAGCGCTTATGGATCAAGCGCTGGCGCCTTATGTGTTAGCACAAGCCTTGGTCGGGTTCATTCTC
TGATTGCATGAATTAACGCC

Downstream 100 bases:

>100_bases
GCGCATGGTGCGCATTGTGGGCGGGCACCTTCCCTGGCAATAGTGGTCAAGCGTCGCCGGACCGTTTCATGCCTGCGGCC
AGGGCGGCCAGTGCCAAAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MFRKLFLPPVADLLAFEAAARHASISRAAEELHLTQSAVSRQVRQLEAQLGQALFTRVRQRVVLTDAGRLYAADVRAVLQ
QLGAATQKAMALADGGGLLHLAVLPTLGARWLVPRLPAFAALHPELTVNLSARGEPFDFAQEPFDAAIHYGAPHWAGAVC
DYLMHEEVVPVCSPALRTRHRIETPADVAGLTLLHQTTRPVLWADWFEQEGVPAPQALRGPRFEQFAMIAQAAISGLGVA
LLPRFLMEEELASGALVELPGGVLTSSDAYYLVVPLARAESAGVKAFRAWLREVAGQRELGRVGG

Sequences:

>Translated_305_residues
MFRKLFLPPVADLLAFEAAARHASISRAAEELHLTQSAVSRQVRQLEAQLGQALFTRVRQRVVLTDAGRLYAADVRAVLQ
QLGAATQKAMALADGGGLLHLAVLPTLGARWLVPRLPAFAALHPELTVNLSARGEPFDFAQEPFDAAIHYGAPHWAGAVC
DYLMHEEVVPVCSPALRTRHRIETPADVAGLTLLHQTTRPVLWADWFEQEGVPAPQALRGPRFEQFAMIAQAAISGLGVA
LLPRFLMEEELASGALVELPGGVLTSSDAYYLVVPLARAESAGVKAFRAWLREVAGQRELGRVGG
>Mature_305_residues
MFRKLFLPPVADLLAFEAAARHASISRAAEELHLTQSAVSRQVRQLEAQLGQALFTRVRQRVVLTDAGRLYAADVRAVLQ
QLGAATQKAMALADGGGLLHLAVLPTLGARWLVPRLPAFAALHPELTVNLSARGEPFDFAQEPFDAAIHYGAPHWAGAVC
DYLMHEEVVPVCSPALRTRHRIETPADVAGLTLLHQTTRPVLWADWFEQEGVPAPQALRGPRFEQFAMIAQAAISGLGVA
LLPRFLMEEELASGALVELPGGVLTSSDAYYLVVPLARAESAGVKAFRAWLREVAGQRELGRVGG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=40.2097902097902, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1786448, Length=300, Percent_Identity=35, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=30.7167235494881, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=30.9859154929577, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=27.972027972028, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=40.5172413793103, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=25.503355704698, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=124, Percent_Identity=33.8709677419355, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=266, Percent_Identity=27.8195488721804, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=258, Percent_Identity=26.7441860465116, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32995; Mature: 32995

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKLFLPPVADLLAFEAAARHASISRAAEELHLTQSAVSRQVRQLEAQLGQALFTRVRQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLTDAGRLYAADVRAVLQQLGAATQKAMALADGGGLLHLAVLPTLGARWLVPRLPAFA
HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCHHHHHCCCHHH
ALHPELTVNLSARGEPFDFAQEPFDAAIHYGAPHWAGAVCDYLMHEEVVPVCSPALRTRH
HCCCCEEEEECCCCCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RIETPADVAGLTLLHQTTRPVLWADWFEQEGVPAPQALRGPRFEQFAMIAQAAISGLGVA
CCCCCHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHH
LLPRFLMEEELASGALVELPGGVLTSSDAYYLVVPLARAESAGVKAFRAWLREVAGQREL
HHHHHHHHHHHHCCCEEECCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHH
GRVGG
CCCCC
>Mature Secondary Structure
MFRKLFLPPVADLLAFEAAARHASISRAAEELHLTQSAVSRQVRQLEAQLGQALFTRVRQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLTDAGRLYAADVRAVLQQLGAATQKAMALADGGGLLHLAVLPTLGARWLVPRLPAFA
HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCHHHHHCCCHHH
ALHPELTVNLSARGEPFDFAQEPFDAAIHYGAPHWAGAVCDYLMHEEVVPVCSPALRTRH
HCCCCEEEEECCCCCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RIETPADVAGLTLLHQTTRPVLWADWFEQEGVPAPQALRGPRFEQFAMIAQAAISGLGVA
CCCCCHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHH
LLPRFLMEEELASGALVELPGGVLTSSDAYYLVVPLARAESAGVKAFRAWLREVAGQREL
HHHHHHHHHHHHCCCEEECCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHH
GRVGG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]