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The map label for this gene is gshA [H]

Identifier: 222110539

GI number: 222110539

Start: 1402967

End: 1404499

Strand: Direct

Name: gshA [H]

Synonym: Dtpsy_1339

Alternate gene names: 222110539

Gene position: 1402967-1404499 (Clockwise)

Preceding gene: 222110538

Following gene: 222110541

Centisome position: 36.95

GC content: 68.95

Gene sequence:

>1533_bases
ATGAGCACACTCCCACAGCGAATCGCAGCCCTGCCCGCCGACCGGCTGGCCGGCATGCGCCGCGGCATCGAGAAGGAGGG
GCTGCGCGTGCTGCCCACGGGCGCCCTGGCGCTCACACCCCATCCGGTGGCGCTGGGCTCGGCCCTCACGCACCCCTGCA
TCACCACGGACTACAGCGAATCGCAGATCGAGCTCATCACCGGTGCCCACCGCACGGTGCAGGAATGCCAGGATGAACTG
ACCGAGATCCACCAGTTCGTGCACCGCGTGCTGCAGCAGCAGCCGGGCGGCGAGATGCTCTGGGTGTCCAGCATGCCCTG
CATGTTGCCTACGGACGAGACCATTCCGTTGGCGCGCTACGGCTCGTCCAACCCGGGGCGTGCCAAGAGCGTGTACCGCA
TGGGCCTGGGCCACCGCTACGGCCGGCGCATGCAGACCATCTCGGGCATTCATTACAACTGGTCGCTGCCCGGCGTGGGC
AGCGACGAGTACTTTGCGCTGATCCGCAACTTCCGCCGGCACGCGTTCGTGCTTCTGTACCTGTTTGGCGCATCGCCTGC
GCTGTGCCCGTGCTTCGTCGAAGGGCGACAACACAGCCTGCAGCCGCTGGGCGACGGCAAGCGCGCGCTGTACCTGCCGC
ATGCCACTTCGCTGCGCATGGGGCGCCTGGGCTACCAGAGCGACGCCCAGGCCACGCTGGCCGTCAGCTACAACGGCCTG
CAGGGCTATGCCGATTCGCTGCACGAGGCGCTCACGCGGCCTTATCCGGCCTACGAGGCGATTGGCGTGCGCAACCCCGG
CGGCGACTACAACCAGATGGGCACCAGCCTGCTGCAGATCGAGAACGAGTTCTACGGCACCATCCGGCCCAAGCGCACCG
TCCACAGCGGCGAGCGCCCGCTGCACGCGCTGCGCGAGCGCGGCGTGGAGTATGTCGAGGTGCGCCTGATGGACCTGGAC
CCGTTTGTGCCCGTGGGCATCACCGCCGACACCATGCGCCTGCTGGACGTGTTCCTGCTGCACTGCCTGCTGTCGGACAG
CCCGCCCGACACGCCGGCCGAGATCGCCGAACTCAAGAACAACCAGCACTTGGCGGCCGAGCGCGGGCGCGAGGCCGGCC
TACAGCTCATGCGCCGCGGCCAGCGTGTGCTCCTGACCGATTGGGCCGCCGAGGTGCTGGCCGAATGCGCGCCCATCGCC
GCCACGCTGGATGCCGCCCACGGCGGCGAGGCTTATGCGCACGCGCTGACGGCGGCCCAGGCGACACTGGCGGCCCCGCA
CTCCACACCGTCCGCGCGTGTGCTGGCCGAGCTGCAACAGCAGCATGACAACGACTTCACCGCCTTCGCACTCGCTCATT
CCCAGTCGGCGCGGGACATCCTGATGGGGCAGCCCTGGAGCGAGTCGCAGCAGGCGCGCTACCAGGCGCTGGCCGAGCGG
TCGCTGGAGGAGCAGCGGGCCATTGAAGTGGCAGATGACCTGCCGTTCGAGGCGTGGCGCGAGCGGTTCATGGCGGTCGA
GCAGTTGGGGTAG

Upstream 100 bases:

>100_bases
TCCCCGGTGTCAGGCTGGCGCTTGATGTGGGCGCGGGCAGGCCGCAGCGCGGCGTGCTAAGTTCCGGCCCCACCTCAAAA
AAGATGGAACAACCACCACC

Downstream 100 bases:

>100_bases
TTTTTTGAGGGCAGCGCTTATGGCGTAAGCGCCGCCAGCTATTCTTTTTGATGTTGCTGCGCGGGGGCGCATAACCCGGT
CAGCACCCAAGCATTCGGCA

Product: glutamate/cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSTLPQRIAALPADRLAGMRRGIEKEGLRVLPTGALALTPHPVALGSALTHPCITTDYSESQIELITGAHRTVQECQDEL
TEIHQFVHRVLQQQPGGEMLWVSSMPCMLPTDETIPLARYGSSNPGRAKSVYRMGLGHRYGRRMQTISGIHYNWSLPGVG
SDEYFALIRNFRRHAFVLLYLFGASPALCPCFVEGRQHSLQPLGDGKRALYLPHATSLRMGRLGYQSDAQATLAVSYNGL
QGYADSLHEALTRPYPAYEAIGVRNPGGDYNQMGTSLLQIENEFYGTIRPKRTVHSGERPLHALRERGVEYVEVRLMDLD
PFVPVGITADTMRLLDVFLLHCLLSDSPPDTPAEIAELKNNQHLAAERGREAGLQLMRRGQRVLLTDWAAEVLAECAPIA
ATLDAAHGGEAYAHALTAAQATLAAPHSTPSARVLAELQQQHDNDFTAFALAHSQSARDILMGQPWSESQQARYQALAER
SLEEQRAIEVADDLPFEAWRERFMAVEQLG

Sequences:

>Translated_510_residues
MSTLPQRIAALPADRLAGMRRGIEKEGLRVLPTGALALTPHPVALGSALTHPCITTDYSESQIELITGAHRTVQECQDEL
TEIHQFVHRVLQQQPGGEMLWVSSMPCMLPTDETIPLARYGSSNPGRAKSVYRMGLGHRYGRRMQTISGIHYNWSLPGVG
SDEYFALIRNFRRHAFVLLYLFGASPALCPCFVEGRQHSLQPLGDGKRALYLPHATSLRMGRLGYQSDAQATLAVSYNGL
QGYADSLHEALTRPYPAYEAIGVRNPGGDYNQMGTSLLQIENEFYGTIRPKRTVHSGERPLHALRERGVEYVEVRLMDLD
PFVPVGITADTMRLLDVFLLHCLLSDSPPDTPAEIAELKNNQHLAAERGREAGLQLMRRGQRVLLTDWAAEVLAECAPIA
ATLDAAHGGEAYAHALTAAQATLAAPHSTPSARVLAELQQQHDNDFTAFALAHSQSARDILMGQPWSESQQARYQALAER
SLEEQRAIEVADDLPFEAWRERFMAVEQLG
>Mature_509_residues
STLPQRIAALPADRLAGMRRGIEKEGLRVLPTGALALTPHPVALGSALTHPCITTDYSESQIELITGAHRTVQECQDELT
EIHQFVHRVLQQQPGGEMLWVSSMPCMLPTDETIPLARYGSSNPGRAKSVYRMGLGHRYGRRMQTISGIHYNWSLPGVGS
DEYFALIRNFRRHAFVLLYLFGASPALCPCFVEGRQHSLQPLGDGKRALYLPHATSLRMGRLGYQSDAQATLAVSYNGLQ
GYADSLHEALTRPYPAYEAIGVRNPGGDYNQMGTSLLQIENEFYGTIRPKRTVHSGERPLHALRERGVEYVEVRLMDLDP
FVPVGITADTMRLLDVFLLHCLLSDSPPDTPAEIAELKNNQHLAAERGREAGLQLMRRGQRVLLTDWAAEVLAECAPIAA
TLDAAHGGEAYAHALTAAQATLAAPHSTPSARVLAELQQQHDNDFTAFALAHSQSARDILMGQPWSESQQARYQALAERS
LEEQRAIEVADDLPFEAWRERFMAVEQLG

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=507, Percent_Identity=38.2642998027613, Blast_Score=337, Evalue=8e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 56370; Mature: 56238

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLPQRIAALPADRLAGMRRGIEKEGLRVLPTGALALTPHPVALGSALTHPCITTDYSE
CCCCHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCEEECCCCHHHHHHHCCCCCCCCCCC
SQIELITGAHRTVQECQDELTEIHQFVHRVLQQQPGGEMLWVSSMPCMLPTDETIPLARY
CCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHCC
GSSNPGRAKSVYRMGLGHRYGRRMQTISGIHYNWSLPGVGSDEYFALIRNFRRHAFVLLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LFGASPALCPCFVEGRQHSLQPLGDGKRALYLPHATSLRMGRLGYQSDAQATLAVSYNGL
HHCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCEEEEEEECCH
QGYADSLHEALTRPYPAYEAIGVRNPGGDYNQMGTSLLQIENEFYGTIRPKRTVHSGERP
HHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
LHALRERGVEYVEVRLMDLDPFVPVGITADTMRLLDVFLLHCLLSDSPPDTPAEIAELKN
HHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
NQHLAAERGREAGLQLMRRGQRVLLTDWAAEVLAECAPIAATLDAAHGGEAYAHALTAAQ
CCCHHHHCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ATLAAPHSTPSARVLAELQQQHDNDFTAFALAHSQSARDILMGQPWSESQQARYQALAER
HHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHH
SLEEQRAIEVADDLPFEAWRERFMAVEQLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLPQRIAALPADRLAGMRRGIEKEGLRVLPTGALALTPHPVALGSALTHPCITTDYSE
CCCHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCEEECCCCHHHHHHHCCCCCCCCCCC
SQIELITGAHRTVQECQDELTEIHQFVHRVLQQQPGGEMLWVSSMPCMLPTDETIPLARY
CCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHCC
GSSNPGRAKSVYRMGLGHRYGRRMQTISGIHYNWSLPGVGSDEYFALIRNFRRHAFVLLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LFGASPALCPCFVEGRQHSLQPLGDGKRALYLPHATSLRMGRLGYQSDAQATLAVSYNGL
HHCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCEEEEEEECCH
QGYADSLHEALTRPYPAYEAIGVRNPGGDYNQMGTSLLQIENEFYGTIRPKRTVHSGERP
HHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
LHALRERGVEYVEVRLMDLDPFVPVGITADTMRLLDVFLLHCLLSDSPPDTPAEIAELKN
HHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
NQHLAAERGREAGLQLMRRGQRVLLTDWAAEVLAECAPIAATLDAAHGGEAYAHALTAAQ
CCCHHHHCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ATLAAPHSTPSARVLAELQQQHDNDFTAFALAHSQSARDILMGQPWSESQQARYQALAER
HHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHH
SLEEQRAIEVADDLPFEAWRERFMAVEQLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA