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The map label for this gene is truB [H]

Identifier: 222110452

GI number: 222110452

Start: 1312862

End: 1313863

Strand: Direct

Name: truB [H]

Synonym: Dtpsy_1249

Alternate gene names: 222110452

Gene position: 1312862-1313863 (Clockwise)

Preceding gene: 222110451

Following gene: 222110453

Centisome position: 34.58

GC content: 66.27

Gene sequence:

>1002_bases
ATGACCGAACGCGCTCCCCGCATCCGGGTGCAGCGGCGCCCGGTGCACGGAGTGCTGCTGCTCGACAAACCCCTGGGCCT
GTCCAGCAACGACGCCTTGCAGAAGGTGAAGTGGCTGCTGCGCGCCGAGAAGGCTGGCCACACGGGCACGCTGGACCCGC
TGGCCAGTGGCGTGCTGCCGCTGTGCTTTGGTGCCGCCACCAAATTCAGCCAGTTGCAGCTGGAAGCGCCCAAGGCCTAT
GAAGCCGTCGCACTGTTGGGTACTACCACAAGCACTGGTGACGCCGAGGGTCAGGTGCTGGCGCAGTGCGCGGTCGATCC
GGCCATGCTCACGCCCGAGCGACTGTTGGCGGTACAGGCCCAGTTCACCGGCCCCATCCGCCAGGTGCCGCCCATGCACA
GCGCGCTCAAGAAAGACGGCAAGGCGCTGTACGAGTACGCGCGTGCCGGCATCGCCGTGGAGCGCGAGGCGCGTGATGTC
ACGATTCATGCATTAAAACTGGCGCTAACGCATACGGAGCAAGCGCAACCAGCTATTAAAATAGTAGTGACCTGCAGCAA
GGGTACCTACATCCGTACCCTGGGTGAAGACATCGGCCAGGCGCTGGGCTGCGGCGCGCATCTCACGTTCCTGCGCCGTA
CCGACACGGGCGGTATCGGCGTGGAGCGCTGCGTGACACTGGAGGCGCTGCAGGCCATGGACGAGGATGCGCGCATGGCC
TGCGTGCACCCGGTGGAGACCTTGCTGGCGCAGCACACGGCTGTCACGCTGGACGATGACAATGCCGGGCGTTTCCTCTC
GGGCATGCGCCGGCGCGGCCCCTGGCCCGACGCGCCCGCCGTGGCCGTGTTCGGGGCGCGCCCGCGCGTGCTGCTGGGGG
TCGGGCATGTGCGGGGAGGAGAGCTGATTCCAGACCGGCTGCTCAGCCCCCTTGAAATTCAACAGATTCTGGAGAGCTCG
CCGCGGGCTGAACGCGGCACATTGGAAGCAACTACGCTATGA

Upstream 100 bases:

>100_bases
CGCTGCACTTCGTGTACGACCGCACCACCGAGAAGGCGGCCGACATGAATGCGCTGATCGCGCGCGCGGTGGCCTCTCGT
TCCAAAGACGACGACGCAGC

Downstream 100 bases:

>100_bases
GCAAACAAATCCGCAACATCGCCATCATCGCTCACGTCGACCATGGCAAGACCACGATGGTGGACCAACTGCTGCGCCAG
TCCGGCACCTTCGCCGAGCA

Product: tRNA pseudouridine synthase b

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTERAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKVKWLLRAEKAGHTGTLDPLASGVLPLCFGAATKFSQLQLEAPKAY
EAVALLGTTTSTGDAEGQVLAQCAVDPAMLTPERLLAVQAQFTGPIRQVPPMHSALKKDGKALYEYARAGIAVEREARDV
TIHALKLALTHTEQAQPAIKIVVTCSKGTYIRTLGEDIGQALGCGAHLTFLRRTDTGGIGVERCVTLEALQAMDEDARMA
CVHPVETLLAQHTAVTLDDDNAGRFLSGMRRRGPWPDAPAVAVFGARPRVLLGVGHVRGGELIPDRLLSPLEIQQILESS
PRAERGTLEATTL

Sequences:

>Translated_333_residues
MTERAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKVKWLLRAEKAGHTGTLDPLASGVLPLCFGAATKFSQLQLEAPKAY
EAVALLGTTTSTGDAEGQVLAQCAVDPAMLTPERLLAVQAQFTGPIRQVPPMHSALKKDGKALYEYARAGIAVEREARDV
TIHALKLALTHTEQAQPAIKIVVTCSKGTYIRTLGEDIGQALGCGAHLTFLRRTDTGGIGVERCVTLEALQAMDEDARMA
CVHPVETLLAQHTAVTLDDDNAGRFLSGMRRRGPWPDAPAVAVFGARPRVLLGVGHVRGGELIPDRLLSPLEIQQILESS
PRAERGTLEATTL
>Mature_332_residues
TERAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKVKWLLRAEKAGHTGTLDPLASGVLPLCFGAATKFSQLQLEAPKAYE
AVALLGTTTSTGDAEGQVLAQCAVDPAMLTPERLLAVQAQFTGPIRQVPPMHSALKKDGKALYEYARAGIAVEREARDVT
IHALKLALTHTEQAQPAIKIVVTCSKGTYIRTLGEDIGQALGCGAHLTFLRRTDTGGIGVERCVTLEALQAMDEDARMAC
VHPVETLLAQHTAVTLDDDNAGRFLSGMRRRGPWPDAPAVAVFGARPRVLLGVGHVRGGELIPDRLLSPLEIQQILESSP
RAERGTLEATTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=242, Percent_Identity=35.1239669421488, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4503337, Length=263, Percent_Identity=28.1368821292776, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI215599015, Length=263, Percent_Identity=28.1368821292776, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=242, Percent_Identity=50, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17553978, Length=252, Percent_Identity=30.1587301587302, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=48.0314960629921, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=252, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=264, Percent_Identity=29.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=264, Percent_Identity=29.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=264, Percent_Identity=29.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=264, Percent_Identity=29.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=264, Percent_Identity=29.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=264, Percent_Identity=29.5454545454545, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35753; Mature: 35622

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKVKWLLRAEKAGHTGTLDPLASGVLP
CCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LCFGAATKFSQLQLEAPKAYEAVALLGTTTSTGDAEGQVLAQCAVDPAMLTPERLLAVQA
HHHHHCCCHHHEEECCCCHHEEEEEEECCCCCCCCCCHHHHHHHCCCHHCCHHHHHEEHH
QFTGPIRQVPPMHSALKKDGKALYEYARAGIAVEREARDVTIHALKLALTHTEQAQPAIK
HHCCCHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEECCCCCCCCEEE
IVVTCSKGTYIRTLGEDIGQALGCGAHLTFLRRTDTGGIGVERCVTLEALQAMDEDARMA
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEE
CVHPVETLLAQHTAVTLDDDNAGRFLSGMRRRGPWPDAPAVAVFGARPRVLLGVGHVRGG
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCC
ELIPDRLLSPLEIQQILESSPRAERGTLEATTL
CCCHHHCCCHHHHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
TERAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKVKWLLRAEKAGHTGTLDPLASGVLP
CCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LCFGAATKFSQLQLEAPKAYEAVALLGTTTSTGDAEGQVLAQCAVDPAMLTPERLLAVQA
HHHHHCCCHHHEEECCCCHHEEEEEEECCCCCCCCCCHHHHHHHCCCHHCCHHHHHEEHH
QFTGPIRQVPPMHSALKKDGKALYEYARAGIAVEREARDVTIHALKLALTHTEQAQPAIK
HHCCCHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEECCCCCCCCEEE
IVVTCSKGTYIRTLGEDIGQALGCGAHLTFLRRTDTGGIGVERCVTLEALQAMDEDARMA
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEE
CVHPVETLLAQHTAVTLDDDNAGRFLSGMRRRGPWPDAPAVAVFGARPRVLLGVGHVRGG
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCC
ELIPDRLLSPLEIQQILESSPRAERGTLEATTL
CCCHHHCCCHHHHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA