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The map label for this gene is purA

Identifier: 222110314

GI number: 222110314

Start: 1148831

End: 1150207

Strand: Direct

Name: purA

Synonym: Dtpsy_1101

Alternate gene names: 222110314

Gene position: 1148831-1150207 (Clockwise)

Preceding gene: 222110313

Following gene: 222110315

Centisome position: 30.26

GC content: 59.19

Gene sequence:

>1377_bases
ATGAAAGCAAGCAAGGGTCGTAACGTTGTCGTGGTTGGCACCCAGTGGGGCGATGAAGGCAAAGGCAAACTGGTTGATTG
GCTGACTGAGAGCGCCCAGGGGGTGGTGCGTTTTCAGGGCGGGCACAACGCTGGTCACACGTTGGTCATCAACGGCGTCA
AGACGGCGCTGCACTTGATCCCTAGCGGGATCATGCGCCCGGGTGTCAAGTGCTACATCGGCAACGGCGTCGTTCTCTCG
GCGGGCAAATTGTTTGAGGAGATCGAGGGACTCGAAAAAGCGGGCGTCGAAGTGCGCTCGCGTCTGCGTGTCAGCGAAGC
TTGTCCATTGATCCTGCCGTTCCATGCGGCGCTTGATGTGGCGCGTGAAGCGGCGCGTGAACACGGTGGCGCCGAGAAGA
TTGGTACCACTGGCCGTGGTATCGGACCGGCCTACGAGGACAAGATCGCTCGCCGTGCCCTGCGCGTACAAGACCTCAAG
TATCCAGAACGCTTTGCCGCCAAGCTGCGCGAATTGCTGGCGTTGCACAACCATGTCCTCAGCACCTTCTTGGGTTCGGC
CAATTTTCAGTTTGGCGATGCACTCAAGCCCTACATCACGGGCGGGCAAGTGCAATTTGAACCGGTCTTTGATGAGGCCA
TGCGGCATGCCGAGATGCTCAAGCCCATGATGGCAGATGTCTCGCGTGAACTGAACGAAGCGCATGCCGCGGGCGCAAAT
CTGCTGTTTGAGGGGGCCCAGGGCACGTTGCTCGATGTGGACCATGGCACCTATCCCTATGTCACGTCCAGTAACTGTGT
GGCGGGTAATGCGGCAGCGGGGTCAGGTGTTGGCCCGGGGCATCTGCACTACATCTTGGGGATCACCAAGGCATATTGCA
CGCGTGTGGGGGGGGGGCCTTTCCCGACCGAGCTGGACTGGGAAACGCCTGGAACACCGGGTTATCACATGAGTACAGTG
GGCGCCGAAAAAGGGGTGACGACAGGGCGCAGCCGTCGCTGCGGTTGGTTTGATGCCGCACTCCTCAAGCGTAGCGCGCA
GGTCAATGGTCTTTCAGGGCTCTGCATCACCAAGCTGGATGTTCTGGACGGGTTGAGAGAACTGTCGCTATGCGTGGGAT
ACGAGTTGGACGGTGAAAGGATCGACCTTCTGCCAATGGGTGCGGAAGAAATCGCACGTTGCGTACCTATCTACGAGAAT
ATTGCCGGTTGGACGGAATCCACCGTGGGCGTGACTCGCTATGAGGACCTGCCGTCCAATGCGCGCCGCTATCTGGAGCG
TATTGAGCAAGTGACGGGGGTGCCTATCGCAATGATCTCGACCAGTCCAGACCGTGATCACACGATCTTGATGCGCCATC
CCTACGCGGCGGACTGA

Upstream 100 bases:

>100_bases
AAGTGGACGAATTCCACTGCGATCGGGAGCTTGTCCTGGTGGATGGCAACTGGGTCGTAAAGGCGATTTGAGTTTCTTCA
GCATTTGAATCGGGTTTGAC

Downstream 100 bases:

>100_bases
TGTTGGAGTGTGTCGCCCGCTGTTAAGGCGATTGTTGCTATCAATTCAGGAGTTAGTATGCTGACGGAAGACGGCAAGCA
TTTGTACGTGAGCTACGACG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKASKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVLS
AGKLFEEIEGLEKAGVEVRSRLRVSEACPLILPFHAALDVAREAAREHGGAEKIGTTGRGIGPAYEDKIARRALRVQDLK
YPERFAAKLRELLALHNHVLSTFLGSANFQFGDALKPYITGGQVQFEPVFDEAMRHAEMLKPMMADVSRELNEAHAAGAN
LLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGHLHYILGITKAYCTRVGGGPFPTELDWETPGTPGYHMSTV
GAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGLRELSLCVGYELDGERIDLLPMGAEEIARCVPIYEN
IAGWTESTVGVTRYEDLPSNARRYLERIEQVTGVPIAMISTSPDRDHTILMRHPYAAD

Sequences:

>Translated_458_residues
MKASKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVLS
AGKLFEEIEGLEKAGVEVRSRLRVSEACPLILPFHAALDVAREAAREHGGAEKIGTTGRGIGPAYEDKIARRALRVQDLK
YPERFAAKLRELLALHNHVLSTFLGSANFQFGDALKPYITGGQVQFEPVFDEAMRHAEMLKPMMADVSRELNEAHAAGAN
LLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGHLHYILGITKAYCTRVGGGPFPTELDWETPGTPGYHMSTV
GAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGLRELSLCVGYELDGERIDLLPMGAEEIARCVPIYEN
IAGWTESTVGVTRYEDLPSNARRYLERIEQVTGVPIAMISTSPDRDHTILMRHPYAAD
>Mature_458_residues
MKASKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVLS
AGKLFEEIEGLEKAGVEVRSRLRVSEACPLILPFHAALDVAREAAREHGGAEKIGTTGRGIGPAYEDKIARRALRVQDLK
YPERFAAKLRELLALHNHVLSTFLGSANFQFGDALKPYITGGQVQFEPVFDEAMRHAEMLKPMMADVSRELNEAHAAGAN
LLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGHLHYILGITKAYCTRVGGGPFPTELDWETPGTPGYHMSTV
GAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGLRELSLCVGYELDGERIDLLPMGAEEIARCVPIYEN
IAGWTESTVGVTRYEDLPSNARRYLERIEQVTGVPIAMISTSPDRDHTILMRHPYAAD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=452, Percent_Identity=39.6017699115044, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI34577063, Length=450, Percent_Identity=38.8888888888889, Blast_Score=308, Evalue=5e-84,
Organism=Homo sapiens, GI40316944, Length=416, Percent_Identity=38.2211538461538, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1790620, Length=453, Percent_Identity=56.5121412803532, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25146553, Length=461, Percent_Identity=40.3470715835141, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI25146555, Length=461, Percent_Identity=40.3470715835141, Blast_Score=311, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6324109, Length=449, Percent_Identity=41.8708240534521, Blast_Score=336, Evalue=4e-93,
Organism=Drosophila melanogaster, GI21356033, Length=451, Percent_Identity=37.9157427937916, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ACIET (B9MFY7)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552578.1
- GeneID:   7382910
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1101
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 49385; Mature: 49385

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 18-18 ACT_SITE 46-46 BINDING 137-137 BINDING 151-151 BINDING 247-247 BINDING 262-262 BINDING 330-330 BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLI
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHH
PSGIMRPGVKCYIGNGVVLSAGKLFEEIEGLEKAGVEVRSRLRVSEACPLILPFHAALDV
HHHHCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHHHHHH
AREAAREHGGAEKIGTTGRGIGPAYEDKIARRALRVQDLKYPERFAAKLRELLALHNHVL
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
STFLGSANFQFGDALKPYITGGQVQFEPVFDEAMRHAEMLKPMMADVSRELNEAHAAGAN
HHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGHLHYILGITKAYCTRVGGGP
EEEECCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
FPTELDWETPGTPGYHMSTVGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLD
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
VLDGLRELSLCVGYELDGERIDLLPMGAEEIARCVPIYENIAGWTESTVGVTRYEDLPSN
HHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCHH
ARRYLERIEQVTGVPIAMISTSPDRDHTILMRHPYAAD
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKASKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLI
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHH
PSGIMRPGVKCYIGNGVVLSAGKLFEEIEGLEKAGVEVRSRLRVSEACPLILPFHAALDV
HHHHCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHHHHHH
AREAAREHGGAEKIGTTGRGIGPAYEDKIARRALRVQDLKYPERFAAKLRELLALHNHVL
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
STFLGSANFQFGDALKPYITGGQVQFEPVFDEAMRHAEMLKPMMADVSRELNEAHAAGAN
HHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGHLHYILGITKAYCTRVGGGP
EEEECCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
FPTELDWETPGTPGYHMSTVGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLD
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
VLDGLRELSLCVGYELDGERIDLLPMGAEEIARCVPIYENIAGWTESTVGVTRYEDLPSN
HHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCHH
ARRYLERIEQVTGVPIAMISTSPDRDHTILMRHPYAAD
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA