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The map label for this gene is hisZ

Identifier: 222110313

GI number: 222110313

Start: 1147653

End: 1148801

Strand: Direct

Name: hisZ

Synonym: Dtpsy_1100

Alternate gene names: 222110313

Gene position: 1147653-1148801 (Clockwise)

Preceding gene: 222110312

Following gene: 222110314

Centisome position: 30.23

GC content: 60.4

Gene sequence:

>1149_bases
ATGTCTGCCTGGGTCCTGCCGGATCATATTGCCGATGTCTTGCCTTCCGAGGCGCGGCACATTGAAGAACTTCGTCGCGG
ACTGCTCGATACGGCACGCAGTTACGGTTATGAGTTGGTGATGCCTCCTTTGCTGGAGCATCTGGAATCACTGTTGACGG
GCACTGGCGAGGCGCTGGACCTCCAAACGTTCAAATTGGTGGACCAACTCTCGGGTCGATCTCTCGGCTTGAGGGCCGAT
ACGACCCAACAGGTCGCACGCATTGATGCGCACCTGCTCAACCGGCAGGGGGTGGCGCGTCTATGCTATTGCGGCCCAGT
ACTGCACACGCGTCCCGATCGTCCGCACGCCACACGGGAGCCCTTGCAGTTTGGAGCCGAGATCTACGGTCATCCAGGTA
TCGAGGCGGATATCGAAGCAGTGTTGCTGTCGTTGGAGTGCTTGCGCAGTGCCCACGCACAGGAGGTGAGCGTAGATCTG
GCCGACGTCCGTATCGTGCGCAGTCTCCTTGCAGGACTTCCTGTCGGTATGCATCAGCTCGCCCAAGTCCACGGAGCGCT
TGCTGCCAAAGATGCCAGCGAACTGGCGTCGTTGACACGCGATTTCCCGTCTGCATCGCGGGAAGGGTTGTTGGCACTGT
TGCAGCTGTATGGCGATGCAACAGTGCTGAATGAGGCCGAAAACTTGTTGAAACCCTTTCCTGGTGCGCGCGAGGCGCTA
TCGGATTTGAGGGCGATTGCGGCACGAATGGACGGTGTGCGGGTCACATTTGATCTGGCAGATCTGCGGGGTTATGCCTA
TTACAGCGGAGCGCGTTTTGCGATTTATGCGCAGGGCGCCAGTGATGCGCTGGTGCGAGGCGGCCGCTATGACGAGGTCG
GCGCGGTCTTCGGCCGTAACAGGCCAGCCGCAGGCTTTAGCTTGGACGTGAAGCAACTGGTGGGTGTGGTGTCGGCTCCG
TCCTTGCGTGCGGCCATCCGGGCGCCCTGGGGCGATGGTGGTGCGCTCAGTGCTGCCATTGCTACGTTGCGTCGTCAGGG
CGAAACCGTGGTGTGTGTGCTGCCGGGGCACGGCAGCGAAGTGGACGAATTCCACTGCGATCGGGAGCTTGTCCTGGTGG
ATGGCAACTGGGTCGTAAAGGCGATTTGA

Upstream 100 bases:

>100_bases
CGGCGCCATTCTGCTGCTCACGTAGCCTGGCCGATTTCGGTCCCGGGGTGGGGCTTATCCATGCCGGTAGAATCAACGTT
TTAACAGCCTGCCCCATCCC

Downstream 100 bases:

>100_bases
GTTTCTTCAGCATTTGAATCGGGTTTGACATGAAAGCAAGCAAGGGTCGTAACGTTGTCGTGGTTGGCACCCAGTGGGGC
GATGAAGGCAAAGGCAAACT

Product: histidyl-tRNA synthetase 2

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSAWVLPDHIADVLPSEARHIEELRRGLLDTARSYGYELVMPPLLEHLESLLTGTGEALDLQTFKLVDQLSGRSLGLRAD
TTQQVARIDAHLLNRQGVARLCYCGPVLHTRPDRPHATREPLQFGAEIYGHPGIEADIEAVLLSLECLRSAHAQEVSVDL
ADVRIVRSLLAGLPVGMHQLAQVHGALAAKDASELASLTRDFPSASREGLLALLQLYGDATVLNEAENLLKPFPGAREAL
SDLRAIAARMDGVRVTFDLADLRGYAYYSGARFAIYAQGASDALVRGGRYDEVGAVFGRNRPAAGFSLDVKQLVGVVSAP
SLRAAIRAPWGDGGALSAAIATLRRQGETVVCVLPGHGSEVDEFHCDRELVLVDGNWVVKAI

Sequences:

>Translated_382_residues
MSAWVLPDHIADVLPSEARHIEELRRGLLDTARSYGYELVMPPLLEHLESLLTGTGEALDLQTFKLVDQLSGRSLGLRAD
TTQQVARIDAHLLNRQGVARLCYCGPVLHTRPDRPHATREPLQFGAEIYGHPGIEADIEAVLLSLECLRSAHAQEVSVDL
ADVRIVRSLLAGLPVGMHQLAQVHGALAAKDASELASLTRDFPSASREGLLALLQLYGDATVLNEAENLLKPFPGAREAL
SDLRAIAARMDGVRVTFDLADLRGYAYYSGARFAIYAQGASDALVRGGRYDEVGAVFGRNRPAAGFSLDVKQLVGVVSAP
SLRAAIRAPWGDGGALSAAIATLRRQGETVVCVLPGHGSEVDEFHCDRELVLVDGNWVVKAI
>Mature_381_residues
SAWVLPDHIADVLPSEARHIEELRRGLLDTARSYGYELVMPPLLEHLESLLTGTGEALDLQTFKLVDQLSGRSLGLRADT
TQQVARIDAHLLNRQGVARLCYCGPVLHTRPDRPHATREPLQFGAEIYGHPGIEADIEAVLLSLECLRSAHAQEVSVDLA
DVRIVRSLLAGLPVGMHQLAQVHGALAAKDASELASLTRDFPSASREGLLALLQLYGDATVLNEAENLLKPFPGAREALS
DLRAIAARMDGVRVTFDLADLRGYAYYSGARFAIYAQGASDALVRGGRYDEVGAVFGRNRPAAGFSLDVKQLVGVVSAPS
LRAAIRAPWGDGGALSAAIATLRRQGETVVCVLPGHGSEVDEFHCDRELVLVDGNWVVKAI

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_ACIET (B9MFY6)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552577.1
- GeneID:   7382909
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1100
- ProtClustDB:   CLSK2784975
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41141; Mature: 41010

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAWVLPDHIADVLPSEARHIEELRRGLLDTARSYGYELVMPPLLEHLESLLTGTGEALD
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCCC
LQTFKLVDQLSGRSLGLRADTTQQVARIDAHLLNRQGVARLCYCGPVLHTRPDRPHATRE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHCCCCCCCCCCCC
PLQFGAEIYGHPGIEADIEAVLLSLECLRSAHAQEVSVDLADVRIVRSLLAGLPVGMHQL
HHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AQVHGALAAKDASELASLTRDFPSASREGLLALLQLYGDATVLNEAENLLKPFPGAREAL
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
SDLRAIAARMDGVRVTFDLADLRGYAYYSGARFAIYAQGASDALVRGGRYDEVGAVFGRN
HHHHHHHHHCCCEEEEEEHHHCCCEEEECCCEEEEEECCCCHHHHCCCCCCHHHHHHCCC
RPAAGFSLDVKQLVGVVSAPSLRAAIRAPWGDGGALSAAIATLRRQGETVVCVLPGHGSE
CCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
VDEFHCDRELVLVDGNWVVKAI
HHHHCCCCEEEEECCCEEEEEC
>Mature Secondary Structure 
SAWVLPDHIADVLPSEARHIEELRRGLLDTARSYGYELVMPPLLEHLESLLTGTGEALD
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCCC
LQTFKLVDQLSGRSLGLRADTTQQVARIDAHLLNRQGVARLCYCGPVLHTRPDRPHATRE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHCCCCCCCCCCCC
PLQFGAEIYGHPGIEADIEAVLLSLECLRSAHAQEVSVDLADVRIVRSLLAGLPVGMHQL
HHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AQVHGALAAKDASELASLTRDFPSASREGLLALLQLYGDATVLNEAENLLKPFPGAREAL
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
SDLRAIAARMDGVRVTFDLADLRGYAYYSGARFAIYAQGASDALVRGGRYDEVGAVFGRN
HHHHHHHHHCCCEEEEEEHHHCCCEEEECCCEEEEEECCCCHHHHCCCCCCHHHHHHCCC
RPAAGFSLDVKQLVGVVSAPSLRAAIRAPWGDGGALSAAIATLRRQGETVVCVLPGHGSE
CCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
VDEFHCDRELVLVDGNWVVKAI
HHHHCCCCEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA