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The map label for this gene is rlmN [H]

Identifier: 222110300

GI number: 222110300

Start: 1134637

End: 1135788

Strand: Direct

Name: rlmN [H]

Synonym: Dtpsy_1087

Alternate gene names: 222110300

Gene position: 1134637-1135788 (Clockwise)

Preceding gene: 222110299

Following gene: 222110301

Centisome position: 29.89

GC content: 60.5

Gene sequence:

>1152_bases
ATGTGGGCGTGGACATCCGTGAACGTTATGACCACGAACCTTCTGGATTTTGACCTGGACGGCCTGGCTGCTTTTTGCGA
GCGGCTGGGAGAAAAACGTTTTCGGGCCACGCAACTGTTTCGCTGGATCCACCAGCGCGGTGCCAGCGATTTTGACCAGA
TGAGCGACCTGGCGAAATCGTTGCGCGAGAAGCTCAGGGGCTGCGCCCATGTGGCAGGGCTGCAAGCCATCAGCGAACAT
GTCTCTGCGGACGGCACGGTGAAGTGGCTGTTCGACGTGGGTGACGGCAACGCCGTGGAGACAGTCTTTATTCCTGAGGA
CGATCGCGGCACCTTGTGCATCTCCTCGCAGGCTGGATGCGCCGTAGGCTGCCGTTTTTGCTCCACCGGTCACCAGGGGT
TCAGCCGTAATCTCACTACTGGCGAAATCCTGGCCCAGCTCTGGTATGCCGAGCACGCACTACGGCAGCGCCGCGGTGAC
GGCGAACGCGTGATTTCCAACGTGGTGATGATGGGAATGGGCGAGCCGCTGCAGAACTATGCTGCACTGGTGCCTGCGCT
GCGTGTCATGCTCGATGACCATGGTTACGGCTTGTCGCGGCGTCGCGTAACCGTGTCTACCTCAGGCGTCGTGCCCATGA
TGGACCGCCTGGCGCAGGATTGTCCTGTGGCGCTCGCTGTTTCGCTGCATGCTCCCAATGACGTGTTGCGCGACAACCTC
GTTCCCCTGAACCGCAAGTACCCGCTGCATGAACTCCTGGCGGCGTGTCGGCGTTACTTGGACCACGCACCGCGGGACTT
CATCACTTTTGAATACTGCATGCTGGAAGGCGTGAACGATCAGCCAGAGCATGCGCGACAGTTGATTGACCTGGTGGGCC
GCAAGGCGGCCGACGGCGGGGTGTCGTGCAAGTTCAATCTCATCCCCTTCAACCCGTTCCCGGCTTCCGGTCTGCGCCGA
TCTCCCCCGGCGGCTGTGACGGCTTTCGCACAATTGTTGAGTGACGCGGGTATCGTCACGACCGTTCGCAAGACGCGCGG
TGACGATATTGATGCGGCATGCGGACAACTGGCTGGCGATGTGAAGGACCGAACACGGGTCAATGAACGCATGGCCAAGC
TCAGGACCATCGAGATCAAGCCGGTCATCTGA

Upstream 100 bases:

>100_bases
AGTGGCCTTCTTCTTCCCTGGCCTGAACATCTACTCGCGCTAATGCAGTGGCTCCGCGTGCCGGGCGGTCCTGGTTTGGC
CGCCCCCGCGCGGATGCGCT

Downstream 100 bases:

>100_bases
CGGCGTGGCGCAACAATTACGGGAGGCTTTCATGGCGAAGTGGCGGATCAATCATGGCGGGTGGCAGGCGATGGCGGCGA
TCGGCATTGCCGTTGTCGTC

Product: radical sam enzyme, cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MWAWTSVNVMTTNLLDFDLDGLAAFCERLGEKRFRATQLFRWIHQRGASDFDQMSDLAKSLREKLRGCAHVAGLQAISEH
VSADGTVKWLFDVGDGNAVETVFIPEDDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEILAQLWYAEHALRQRRGD
GERVISNVVMMGMGEPLQNYAALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMMDRLAQDCPVALAVSLHAPNDVLRDNL
VPLNRKYPLHELLAACRRYLDHAPRDFITFEYCMLEGVNDQPEHARQLIDLVGRKAADGGVSCKFNLIPFNPFPASGLRR
SPPAAVTAFAQLLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRVNERMAKLRTIEIKPVI

Sequences:

>Translated_383_residues
MWAWTSVNVMTTNLLDFDLDGLAAFCERLGEKRFRATQLFRWIHQRGASDFDQMSDLAKSLREKLRGCAHVAGLQAISEH
VSADGTVKWLFDVGDGNAVETVFIPEDDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEILAQLWYAEHALRQRRGD
GERVISNVVMMGMGEPLQNYAALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMMDRLAQDCPVALAVSLHAPNDVLRDNL
VPLNRKYPLHELLAACRRYLDHAPRDFITFEYCMLEGVNDQPEHARQLIDLVGRKAADGGVSCKFNLIPFNPFPASGLRR
SPPAAVTAFAQLLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRVNERMAKLRTIEIKPVI
>Mature_383_residues
MWAWTSVNVMTTNLLDFDLDGLAAFCERLGEKRFRATQLFRWIHQRGASDFDQMSDLAKSLREKLRGCAHVAGLQAISEH
VSADGTVKWLFDVGDGNAVETVFIPEDDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEILAQLWYAEHALRQRRGD
GERVISNVVMMGMGEPLQNYAALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMMDRLAQDCPVALAVSLHAPNDVLRDNL
VPLNRKYPLHELLAACRRYLDHAPRDFITFEYCMLEGVNDQPEHARQLIDLVGRKAADGGVSCKFNLIPFNPFPASGLRR
SPPAAVTAFAQLLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRVNERMAKLRTIEIKPVI

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=374, Percent_Identity=51.6042780748663, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42283; Mature: 42283

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWAWTSVNVMTTNLLDFDLDGLAAFCERLGEKRFRATQLFRWIHQRGASDFDQMSDLAKS
CCCEEEEHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LREKLRGCAHVAGLQAISEHVSADGTVKWLFDVGDGNAVETVFIPEDDRGTLCISSQAGC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEECCCCH
AVGCRFCSTGHQGFSRNLTTGEILAQLWYAEHALRQRRGDGERVISNVVMMGMGEPLQNY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
AALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMMDRLAQDCPVALAVSLHAPNDVLRDNL
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCHHHHHCCC
VPLNRKYPLHELLAACRRYLDHAPRDFITFEYCMLEGVNDQPEHARQLIDLVGRKAADGG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
VSCKFNLIPFNPFPASGLRRSPPAAVTAFAQLLSDAGIVTTVRKTRGDDIDAACGQLAGD
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHCC
VKDRTRVNERMAKLRTIEIKPVI
HHHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MWAWTSVNVMTTNLLDFDLDGLAAFCERLGEKRFRATQLFRWIHQRGASDFDQMSDLAKS
CCCEEEEHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LREKLRGCAHVAGLQAISEHVSADGTVKWLFDVGDGNAVETVFIPEDDRGTLCISSQAGC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEECCCCH
AVGCRFCSTGHQGFSRNLTTGEILAQLWYAEHALRQRRGDGERVISNVVMMGMGEPLQNY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
AALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMMDRLAQDCPVALAVSLHAPNDVLRDNL
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCHHHHHCCC
VPLNRKYPLHELLAACRRYLDHAPRDFITFEYCMLEGVNDQPEHARQLIDLVGRKAADGG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
VSCKFNLIPFNPFPASGLRRSPPAAVTAFAQLLSDAGIVTTVRKTRGDDIDAACGQLAGD
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHCC
VKDRTRVNERMAKLRTIEIKPVI
HHHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA