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The map label for this gene is cobB [H]

Identifier: 222110227

GI number: 222110227

Start: 1054113

End: 1055471

Strand: Direct

Name: cobB [H]

Synonym: Dtpsy_1013

Alternate gene names: 222110227

Gene position: 1054113-1055471 (Clockwise)

Preceding gene: 222110226

Following gene: 222110228

Centisome position: 27.76

GC content: 71.96

Gene sequence:

>1359_bases
ATGAGCCGCTGTCCCGCCCTGGTCATCGCCGCGCCCGCCTCTGGGCAGGGCAAGACCACCGTCACAGCCGCTTTGGCGCG
CCTGCATACACGCCAAGGCCGGCGCGTGCGGGTGTTCAAGTGTGGGCCCGACTTCCTGGACCCGCACTGGCACCAATTGG
CCAGCGGCGCCCCCGTGCACCAGCTCGATCTGTGGATGACCGGTGCGGCAGATTGCGCGCAGCGCCTGCGCGCCGCGGCG
GCCGAAGCAGACCTGCTGCTCATCGAAGGGGTGATGGGGTTGTTCGACGGCGAACACAGCGTGGGCGACCTGGCGCAGCG
CTTTGGCATACCGGTGCTTGCGGTGGTGGATGCCTCTGCCATGGGGGGCACGCTGGGCGCCATCGTGCATGGGCTGCGGT
ACTACCGTGCGGGCCTGCCATGGGCCGGTGTCTTGGCCAACCGCGTGGCGGGCGACCGCCATGCGCAGATGCTGCGGGAC
GGCTTGGCCGACGCTGCGGACTGGCTGGGTGCGCTGCCACGGGTCGTGGGTGACGCTGCGGGCGGGAGGGAGCCTGGTGG
CCTTCTGCCCGAGCGGCACTTGGGACTGGTGGCAGCGCACGAACTGCCCGATGCCCTGCAGCGGCTGGACGCCGTGGCCG
ATGCGCTGGCCGGCACGCCCCTGGGCCGCATGTCCGCCGCGGACCTGCAGCGCTGGGCGGTGGACTTCGGCGGCACGCCC
GTGGGCCGTGCCACCATCACGCCGCTGCTGGCGGGCCGCACTGTGGCCGTGGCGGCGGATGAGGCTTTCTGCTTCATCTA
CGAAGCCAATATCGACACCCTGCAGCAGCTGGGCGCGCGCGTGGTGCGCTTTTCGCCGCTGCGCGATGCTGCGCTGCCGG
CCTGCGACGCGCTGTGGCTGCCCGGCGGCTACCCCGAGCTGCACGCCGATGCGCTGGCCGCCAATACGGCCATGCGCGAT
AGCCTGCAGGCGCACGTGACGGCGGGTCGGCCCGTGTGGGCGGAGTGCGGCGGCATGATGGCGCTGCTGGAGTCCATCAC
CCTGGCCGACGGGCGCCGCGTGCCCCTGTGGGGGCTGCTGCCGGGTCAAGCCACCATGCAGCGGCGCCTGGCCGCGCTGG
GGCCGCGCGCGCTGCGCATGGGTACGCATGAGTTGCGCGGCCACACCTTTCACTACTCGATCACCGACAGCCCGGCCGCC
GTGTGCGCACGCGCCCAGCGTCCCGATCACGCAGGGGCACCGGACGGCGGTGAAGCCGTGTACCGCCACGGCAGCATCCA
CGCGAGTTACTTTCACGCATGGTTTGCATCCAGCCCGCAGGCGACCGCAGCCTTGCTGGGCGCGGAGGCGGGGCCATGA

Upstream 100 bases:

>100_bases
AAGGACTACCAGTTGGCGCGCACCTACGCCACCGGCGGCCGCACGTTCTACGTGGGCCTGAAGTGGGCGCCGCAGTAACG
CCATCACCGACTGCCACACC

Downstream 100 bases:

>100_bases
ACGCGGCGCCGCAGACCATCGCCCGCAGTGAACTCATCCTGGGTGGACAAAAGAGCGGCAAGTCGCGCCGCGCCGAACTG
CTGGCGCGCCAGTGGCTGCA

Product: cobb/cobq domain-containing protein glutamine amidotransferase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSRCPALVIAAPASGQGKTTVTAALARLHTRQGRRVRVFKCGPDFLDPHWHQLASGAPVHQLDLWMTGAADCAQRLRAAA
AEADLLLIEGVMGLFDGEHSVGDLAQRFGIPVLAVVDASAMGGTLGAIVHGLRYYRAGLPWAGVLANRVAGDRHAQMLRD
GLADAADWLGALPRVVGDAAGGREPGGLLPERHLGLVAAHELPDALQRLDAVADALAGTPLGRMSAADLQRWAVDFGGTP
VGRATITPLLAGRTVAVAADEAFCFIYEANIDTLQQLGARVVRFSPLRDAALPACDALWLPGGYPELHADALAANTAMRD
SLQAHVTAGRPVWAECGGMMALLESITLADGRRVPLWGLLPGQATMQRRLAALGPRALRMGTHELRGHTFHYSITDSPAA
VCARAQRPDHAGAPDGGEAVYRHGSIHASYFHAWFASSPQATAALLGAEAGP

Sequences:

>Translated_452_residues
MSRCPALVIAAPASGQGKTTVTAALARLHTRQGRRVRVFKCGPDFLDPHWHQLASGAPVHQLDLWMTGAADCAQRLRAAA
AEADLLLIEGVMGLFDGEHSVGDLAQRFGIPVLAVVDASAMGGTLGAIVHGLRYYRAGLPWAGVLANRVAGDRHAQMLRD
GLADAADWLGALPRVVGDAAGGREPGGLLPERHLGLVAAHELPDALQRLDAVADALAGTPLGRMSAADLQRWAVDFGGTP
VGRATITPLLAGRTVAVAADEAFCFIYEANIDTLQQLGARVVRFSPLRDAALPACDALWLPGGYPELHADALAANTAMRD
SLQAHVTAGRPVWAECGGMMALLESITLADGRRVPLWGLLPGQATMQRRLAALGPRALRMGTHELRGHTFHYSITDSPAA
VCARAQRPDHAGAPDGGEAVYRHGSIHASYFHAWFASSPQATAALLGAEAGP
>Mature_451_residues
SRCPALVIAAPASGQGKTTVTAALARLHTRQGRRVRVFKCGPDFLDPHWHQLASGAPVHQLDLWMTGAADCAQRLRAAAA
EADLLLIEGVMGLFDGEHSVGDLAQRFGIPVLAVVDASAMGGTLGAIVHGLRYYRAGLPWAGVLANRVAGDRHAQMLRDG
LADAADWLGALPRVVGDAAGGREPGGLLPERHLGLVAAHELPDALQRLDAVADALAGTPLGRMSAADLQRWAVDFGGTPV
GRATITPLLAGRTVAVAADEAFCFIYEANIDTLQQLGARVVRFSPLRDAALPACDALWLPGGYPELHADALAANTAMRDS
LQAHVTAGRPVWAECGGMMALLESITLADGRRVPLWGLLPGQATMQRRLAALGPRALRMGTHELRGHTFHYSITDSPAAV
CARAQRPDHAGAPDGGEAVYRHGSIHASYFHAWFASSPQATAALLGAEAGP

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47452; Mature: 47321

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCPALVIAAPASGQGKTTVTAALARLHTRQGRRVRVFKCGPDFLDPHWHQLASGAPVH
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCE
QLDLWMTGAADCAQRLRAAAAEADLLLIEGVMGLFDGEHSVGDLAQRFGIPVLAVVDASA
EEHEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCHH
MGGTLGAIVHGLRYYRAGLPWAGVLANRVAGDRHAQMLRDGLADAADWLGALPRVVGDAA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGREPGGLLPERHLGLVAAHELPDALQRLDAVADALAGTPLGRMSAADLQRWAVDFGGTP
CCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
VGRATITPLLAGRTVAVAADEAFCFIYEANIDTLQQLGARVVRFSPLRDAALPACDALWL
CCHHHHHHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHHHEEECCCHHHHCCCCCEEEC
PGGYPELHADALAANTAMRDSLQAHVTAGRPVWAECGGMMALLESITLADGRRVPLWGLL
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PGQATMQRRLAALGPRALRMGTHELRGHTFHYSITDSPAAVCARAQRPDHAGAPDGGEAV
CCHHHHHHHHHHHCHHHHHCCCHHHCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHH
YRHGSIHASYFHAWFASSPQATAALLGAEAGP
EECCCEEHHHHHHHHCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
SRCPALVIAAPASGQGKTTVTAALARLHTRQGRRVRVFKCGPDFLDPHWHQLASGAPVH
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCE
QLDLWMTGAADCAQRLRAAAAEADLLLIEGVMGLFDGEHSVGDLAQRFGIPVLAVVDASA
EEHEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCHH
MGGTLGAIVHGLRYYRAGLPWAGVLANRVAGDRHAQMLRDGLADAADWLGALPRVVGDAA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGREPGGLLPERHLGLVAAHELPDALQRLDAVADALAGTPLGRMSAADLQRWAVDFGGTP
CCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
VGRATITPLLAGRTVAVAADEAFCFIYEANIDTLQQLGARVVRFSPLRDAALPACDALWL
CCHHHHHHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHHHEEECCCHHHHCCCCCEEEC
PGGYPELHADALAANTAMRDSLQAHVTAGRPVWAECGGMMALLESITLADGRRVPLWGLL
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PGQATMQRRLAALGPRALRMGTHELRGHTFHYSITDSPAAVCARAQRPDHAGAPDGGEAV
CCHHHHHHHHHHHCHHHHHCCCHHHCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHH
YRHGSIHASYFHAWFASSPQATAALLGAEAGP
EECCCEEHHHHHHHHCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA