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The map label for this gene is pmbA [H]

Identifier: 222110185

GI number: 222110185

Start: 1007698

End: 1009107

Strand: Direct

Name: pmbA [H]

Synonym: Dtpsy_0971

Alternate gene names: 222110185

Gene position: 1007698-1009107 (Clockwise)

Preceding gene: 222110180

Following gene: 222110186

Centisome position: 26.54

GC content: 68.37

Gene sequence:

>1410_bases
ATGAATACATCCAGCAACCGCGCCGCCAGCGGCACTTCTTCCCAGCCCGACAGCGGCTTCAGCTATACCCGAGAGTTCTT
TGAAGGCCTGGTGGACCATGCCCTGGCCCATGCCAAGAAGTTGGGTGCTACCGACGCGGGGGCGGACGCCTCGGAAGGCT
GCGGCCTGTCGGTGAACGTACGCAAGGGCGAGCTGGAGACGGTGGAGCGCAACCGCGACAAGTCGCTGGGCGTGACGGTG
TACGTGGGCCACCGCCGTGGCAATGCGAGTACGTCCGACTTCTCACTGGCCGCCGTGGAGCGCACGGTGCAGGCGGCCTA
CGACATCGCCCGCTTCACCGCCGAGGACCCGGTGGCCGGCCTGCCCGACGCGGCCGACATCGCGCCACAGGAAACGCACC
GCGACCTGCAACTGTTCCACCCCTGGGCCATCACCAGCGAAGAGGCCGCGCTTCTCGCGCTGGAGTGCGAGGCCGCCGCC
TTCAAGACCCACAAACGCATCACCAACAGCGAAGGCGCGGGCGTGTCGGCGCAGCAGAGCCATTTCTTCAGCGCCCACAC
ACGCGGCTTTCGCGGCGGCTACGCCAGTTCGCGCCACAGCATCTCGGTGGCGCCCATCGCCTCGCTGCCGGGGCGCAATG
CCGAGATGCAGCGCGACGCCTGGTACAGCTCCATGCGCGACGCGCGCGAACTGGCCGCGCCCGAGGCCGTGGGCCGCTAC
GCCGCCCAGCGCGCGTTGAGCCGCCTGGGCAGCCGCAAGATCCCCACCACCGAATGCCCGGTGCTGTTCGAATCGCCCCT
GGCCGCCGGCCTGCTGGGCGGCTTCGTGCAGGCCGTGAGCGGCGGCGCGCTGTACCGCAAGAGCAGCTTCCTCGTCGATT
CGCTGGGCAAGCAGGTGTTCGCCAAGCACATCGACGTGGCCGAGGACCCGTTCATCCTGGGCGGCAAGGGCAGCTCGCCG
TTCGACGATGAGGGCGTGCGCGTGGCGCCGCGCCAGGTGGTGGAGGCGGGCCGCGTGCAGGGCTATTTCCTCAGCACCTA
TTCGGCGCGCAAGCTCGGCATGCCGACCACGGGTAACGCCGGCGGCTCGCACAACCTGGTGCTGTCTTCGCGCCTTACGA
AGCCCGGCGACGACCTGGACGCCATGCTGCAAAAGCTGGGCACGGGCCTGTTCGTGATCGAGCTCATGGGCCAGGGCGTG
AACTACGTGACGGGCGACTACTCGCGCGGCGCCAGCGGCTTCTGGGTGGAGAACGGCCGCATCGCTTATCCGGTGCACGA
GATCACCATCGCCGGCAACCTCAAAGACATGCTGCGCGGCATCGAGGCCGTGGGTGCCGACGCCTACACCTATGGCGCCA
AGACGGTGGGCTCCATCCTCATCAACCGCATGAAGGTGGCGGGCAGTTGA

Upstream 100 bases:

>100_bases
GACATGGGTGTGCTGGGGGCGGAAACAGGCCCTGCGCGGCGCAGCGGCAATGGCGCCTGCCGCGCGGCGGCGCGGGTCGG
CAAGTATCATAGCCGCCGCT

Downstream 100 bases:

>100_bases
GCCGCGCCACGCTGGCAGTCCGCGCATGAGCACGTCGCTGTCGCGGGCCGACTGGGCCAAGGCGCTGGTGGTGGTTGTGG
TCTGGGGGCTGAACTTCGTC

Product: peptidase u62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MNTSSNRAASGTSSQPDSGFSYTREFFEGLVDHALAHAKKLGATDAGADASEGCGLSVNVRKGELETVERNRDKSLGVTV
YVGHRRGNASTSDFSLAAVERTVQAAYDIARFTAEDPVAGLPDAADIAPQETHRDLQLFHPWAITSEEAALLALECEAAA
FKTHKRITNSEGAGVSAQQSHFFSAHTRGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSSMRDARELAAPEAVGRY
AAQRALSRLGSRKIPTTECPVLFESPLAAGLLGGFVQAVSGGALYRKSSFLVDSLGKQVFAKHIDVAEDPFILGGKGSSP
FDDEGVRVAPRQVVEAGRVQGYFLSTYSARKLGMPTTGNAGGSHNLVLSSRLTKPGDDLDAMLQKLGTGLFVIELMGQGV
NYVTGDYSRGASGFWVENGRIAYPVHEITIAGNLKDMLRGIEAVGADAYTYGAKTVGSILINRMKVAGS

Sequences:

>Translated_469_residues
MNTSSNRAASGTSSQPDSGFSYTREFFEGLVDHALAHAKKLGATDAGADASEGCGLSVNVRKGELETVERNRDKSLGVTV
YVGHRRGNASTSDFSLAAVERTVQAAYDIARFTAEDPVAGLPDAADIAPQETHRDLQLFHPWAITSEEAALLALECEAAA
FKTHKRITNSEGAGVSAQQSHFFSAHTRGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSSMRDARELAAPEAVGRY
AAQRALSRLGSRKIPTTECPVLFESPLAAGLLGGFVQAVSGGALYRKSSFLVDSLGKQVFAKHIDVAEDPFILGGKGSSP
FDDEGVRVAPRQVVEAGRVQGYFLSTYSARKLGMPTTGNAGGSHNLVLSSRLTKPGDDLDAMLQKLGTGLFVIELMGQGV
NYVTGDYSRGASGFWVENGRIAYPVHEITIAGNLKDMLRGIEAVGADAYTYGAKTVGSILINRMKVAGS
>Mature_469_residues
MNTSSNRAASGTSSQPDSGFSYTREFFEGLVDHALAHAKKLGATDAGADASEGCGLSVNVRKGELETVERNRDKSLGVTV
YVGHRRGNASTSDFSLAAVERTVQAAYDIARFTAEDPVAGLPDAADIAPQETHRDLQLFHPWAITSEEAALLALECEAAA
FKTHKRITNSEGAGVSAQQSHFFSAHTRGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSSMRDARELAAPEAVGRY
AAQRALSRLGSRKIPTTECPVLFESPLAAGLLGGFVQAVSGGALYRKSSFLVDSLGKQVFAKHIDVAEDPFILGGKGSSP
FDDEGVRVAPRQVVEAGRVQGYFLSTYSARKLGMPTTGNAGGSHNLVLSSRLTKPGDDLDAMLQKLGTGLFVIELMGQGV
NYVTGDYSRGASGFWVENGRIAYPVHEITIAGNLKDMLRGIEAVGADAYTYGAKTVGSILINRMKVAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=47.3094170403587, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1789640, Length=411, Percent_Identity=24.5742092457421, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49786; Mature: 49786

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSSNRAASGTSSQPDSGFSYTREFFEGLVDHALAHAKKLGATDAGADASEGCGLSVNV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RKGELETVERNRDKSLGVTVYVGHRRGNASTSDFSLAAVERTVQAAYDIARFTAEDPVAG
CCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPDAADIAPQETHRDLQLFHPWAITSEEAALLALECEAAAFKTHKRITNSEGAGVSAQQS
CCCHHCCCCHHHCCCEEEECCEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCHHHH
HFFSAHTRGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSSMRDARELAAPEAVGRY
HHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
AAQRALSRLGSRKIPTTECPVLFESPLAAGLLGGFVQAVSGGALYRKSSFLVDSLGKQVF
HHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AKHIDVAEDPFILGGKGSSPFDDEGVRVAPRQVVEAGRVQGYFLSTYSARKLGMPTTGNA
HHHCCCCCCCEEEECCCCCCCCCCCCEECHHHHHHHCCCCEEEEECCCHHHCCCCCCCCC
GGSHNLVLSSRLTKPGDDLDAMLQKLGTGLFVIELMGQGVNYVTGDYSRGASGFWVENGR
CCCCCEEEECCCCCCCCCHHHHHHHHCCCEEEEEHHCCCCCEEECCCCCCCCCEEEECCE
IAYPVHEITIAGNLKDMLRGIEAVGADAYTYGAKTVGSILINRMKVAGS
EEECEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTSSNRAASGTSSQPDSGFSYTREFFEGLVDHALAHAKKLGATDAGADASEGCGLSVNV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RKGELETVERNRDKSLGVTVYVGHRRGNASTSDFSLAAVERTVQAAYDIARFTAEDPVAG
CCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPDAADIAPQETHRDLQLFHPWAITSEEAALLALECEAAAFKTHKRITNSEGAGVSAQQS
CCCHHCCCCHHHCCCEEEECCEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCHHHH
HFFSAHTRGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSSMRDARELAAPEAVGRY
HHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
AAQRALSRLGSRKIPTTECPVLFESPLAAGLLGGFVQAVSGGALYRKSSFLVDSLGKQVF
HHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AKHIDVAEDPFILGGKGSSPFDDEGVRVAPRQVVEAGRVQGYFLSTYSARKLGMPTTGNA
HHHCCCCCCCEEEECCCCCCCCCCCCEECHHHHHHHCCCCEEEEECCCHHHCCCCCCCCC
GGSHNLVLSSRLTKPGDDLDAMLQKLGTGLFVIELMGQGVNYVTGDYSRGASGFWVENGR
CCCCCEEEECCCCCCCCCHHHHHHHHCCCEEEEEHHCCCCCEEECCCCCCCCCEEEECCE
IAYPVHEITIAGNLKDMLRGIEAVGADAYTYGAKTVGSILINRMKVAGS
EEECEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]