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The map label for this gene is bepF [H]

Identifier: 222110155

GI number: 222110155

Start: 965192

End: 966451

Strand: Direct

Name: bepF [H]

Synonym: Dtpsy_0941

Alternate gene names: 222110155

Gene position: 965192-966451 (Clockwise)

Preceding gene: 222110154

Following gene: 222110156

Centisome position: 25.42

GC content: 71.75

Gene sequence:

>1260_bases
ATGAATTCACCATTTTTCTCGTCGTCCCGTCGCCGCTGGTGGACCGCCGCCGGCGTGTCCCTTGCCGTGGCCGTAGCCGG
GGGAACCGTGCTGGGCCTGCAAAGTTCGCATGCCGAGGCGCCCGCCGCTGGCACGGCCCCGCAGGCCATGCCGGTCTCTG
TGGCGGCCGTGCTGCAGCAGGACATTGCGCTGTGGGACGAGTTCTCCGGCCGGCTGGAAGCCGTGCAGCGCGTGGACATT
CGCCCCCGTGTGGCGGGCGCCGTGCAGGCGGTGCATTTCCGCGAGGGCGCACTGGTGAAGCAGGGCGACCTGCTCTTCAC
CGTGGACCCTGCGCCCTACGCGGCCGAGGTGGACCGTGCGGAGGCACAGGTCGTCGCCGCGCAGGCCCGCGTGTCCTACA
CCCGCAGCGAGATGGAGCGCGCCACGCGCCTGTGGGAAGAGCGCGCCATTGCCCAGCGCGAGCACGACGAGCGTGCCAAC
GCCCAGCGCGAGGCCGAGGCCAACCTGCGCGCGGCCCAGGCGGCGCTGCAGACCGCGCGGCTGAACTTGGGCTACACGCA
GGTGCGCGCGCCCGTCGCGGGGCGGGTGGGCCGCATCGAGGTCACGGTGGGCAACTTGGTGGCGGCGGGGGCCGGCGCGC
CGGTGCTTACCACGCTGGTCTCGGTGAGCCCGATCTATGCGAGCTTCGACACTGACGAGCAGGTGGTTGCGCGTGCGCTG
GAAGACCTGCGCACCAGCACGCGCTCCAGTGGCAACGCACGTGCCGCGGTGGACCGCATACCGGTGCAGATGGGCACGGG
CACCACGGGTGGCACGCCGCACGTGGGGCACCTGCAACTCATCGACAACCAGGTGGACGCCCGCAGCGGCACGGTGCGCG
TGCGCGCGGTGTTCGACAACGCCGACGGCACGCTCATGCCGGGCCAGTTCGCACGCATCCGCATGGGCCAGGCGCACCGC
TCCTCGGCCGTGCTCATCAACGAGCGCGCCGTGGGCACGGACCAGAACAAGAAGTTCGTGATGGTGGCGGGCGAGGGCGA
CAAGGCCGAATACCGCGAAGTGCACCTGGGGGTGCACGTGGATGGGCTGCGCGTCGTCACCTCGGGCCTCAAGGCTGGCG
AGCGCATTGTCGTGAATGGCCTGCAGCGCGTGCGCCCCGGCGTGCAGGTGGCGCCGCAGGAGGTGCCCATGGCCGCCAAG
GCCGAACTGGACGGCAACGGCGCGCAGGCGCGCGCCACGCCCAAGCAGCCCGCTGCCTGA

Upstream 100 bases:

>100_bases
TTCCGGCTTTGCGTGCCGGAGGAGAAAGCCTTGCCTGAATCTGCCGCCGCGCACTGCGAGCGGCCTGTCGTTGATTGCCG
AAAGGCCTGGAGGAATCACC

Downstream 100 bases:

>100_bases
GGGGGCGGCGCAACGAAGGACCTTCACCATGAACCTGTCCCGCTTCTTCATCGACCGCCCGATCTTCGCGGGCGTACTGT
CGGTGCTCATCTTTCTTGCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNSPFFSSSRRRWWTAAGVSLAVAVAGGTVLGLQSSHAEAPAAGTAPQAMPVSVAAVLQQDIALWDEFSGRLEAVQRVDI
RPRVAGAVQAVHFREGALVKQGDLLFTVDPAPYAAEVDRAEAQVVAAQARVSYTRSEMERATRLWEERAIAQREHDERAN
AQREAEANLRAAQAALQTARLNLGYTQVRAPVAGRVGRIEVTVGNLVAAGAGAPVLTTLVSVSPIYASFDTDEQVVARAL
EDLRTSTRSSGNARAAVDRIPVQMGTGTTGGTPHVGHLQLIDNQVDARSGTVRVRAVFDNADGTLMPGQFARIRMGQAHR
SSAVLINERAVGTDQNKKFVMVAGEGDKAEYREVHLGVHVDGLRVVTSGLKAGERIVVNGLQRVRPGVQVAPQEVPMAAK
AELDGNGAQARATPKQPAA

Sequences:

>Translated_419_residues
MNSPFFSSSRRRWWTAAGVSLAVAVAGGTVLGLQSSHAEAPAAGTAPQAMPVSVAAVLQQDIALWDEFSGRLEAVQRVDI
RPRVAGAVQAVHFREGALVKQGDLLFTVDPAPYAAEVDRAEAQVVAAQARVSYTRSEMERATRLWEERAIAQREHDERAN
AQREAEANLRAAQAALQTARLNLGYTQVRAPVAGRVGRIEVTVGNLVAAGAGAPVLTTLVSVSPIYASFDTDEQVVARAL
EDLRTSTRSSGNARAAVDRIPVQMGTGTTGGTPHVGHLQLIDNQVDARSGTVRVRAVFDNADGTLMPGQFARIRMGQAHR
SSAVLINERAVGTDQNKKFVMVAGEGDKAEYREVHLGVHVDGLRVVTSGLKAGERIVVNGLQRVRPGVQVAPQEVPMAAK
AELDGNGAQARATPKQPAA
>Mature_419_residues
MNSPFFSSSRRRWWTAAGVSLAVAVAGGTVLGLQSSHAEAPAAGTAPQAMPVSVAAVLQQDIALWDEFSGRLEAVQRVDI
RPRVAGAVQAVHFREGALVKQGDLLFTVDPAPYAAEVDRAEAQVVAAQARVSYTRSEMERATRLWEERAIAQREHDERAN
AQREAEANLRAAQAALQTARLNLGYTQVRAPVAGRVGRIEVTVGNLVAAGAGAPVLTTLVSVSPIYASFDTDEQVVARAL
EDLRTSTRSSGNARAAVDRIPVQMGTGTTGGTPHVGHLQLIDNQVDARSGTVRVRAVFDNADGTLMPGQFARIRMGQAHR
SSAVLINERAVGTDQNKKFVMVAGEGDKAEYREVHLGVHVDGLRVVTSGLKAGERIVVNGLQRVRPGVQVAPQEVPMAAK
AELDGNGAQARATPKQPAA

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=352, Percent_Identity=35.5113636363636, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789929, Length=370, Percent_Identity=31.0810810810811, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=31.3513513513514, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082036, Length=369, Percent_Identity=29.2682926829268, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=25.0755287009063, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081951, Length=170, Percent_Identity=30.5882352941176, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789637, Length=139, Percent_Identity=33.0935251798561, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44469; Mature: 44469

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPFFSSSRRRWWTAAGVSLAVAVAGGTVLGLQSSHAEAPAAGTAPQAMPVSVAAVLQQ
CCCCCCCCCCCCEEEECCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
DIALWDEFSGRLEAVQRVDIRPRVAGAVQAVHFREGALVKQGDLLFTVDPAPYAAEVDRA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCEEECCCEEEEECCCCCHHHHHHH
EAQVVAAQARVSYTRSEMERATRLWEERAIAQREHDERANAQREAEANLRAAQAALQTAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLGYTQVRAPVAGRVGRIEVTVGNLVAAGAGAPVLTTLVSVSPIYASFDTDEQVVARAL
CCCCHHHHCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHCCEEEECCCCHHHHHHHH
EDLRTSTRSSGNARAAVDRIPVQMGTGTTGGTPHVGHLQLIDNQVDARSGTVRVRAVFDN
HHHHHHHCCCCCCEEHHHHCCEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEC
ADGTLMPGQFARIRMGQAHRSSAVLINERAVGTDQNKKFVMVAGEGDKAEYREVHLGVHV
CCCCCCCCCHHEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEE
DGLRVVTSGLKAGERIVVNGLQRVRPGVQVAPQEVPMAAKAELDGNGAQARATPKQPAA
CHHHHHHHHHCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSPFFSSSRRRWWTAAGVSLAVAVAGGTVLGLQSSHAEAPAAGTAPQAMPVSVAAVLQQ
CCCCCCCCCCCCEEEECCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
DIALWDEFSGRLEAVQRVDIRPRVAGAVQAVHFREGALVKQGDLLFTVDPAPYAAEVDRA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCEEECCCEEEEECCCCCHHHHHHH
EAQVVAAQARVSYTRSEMERATRLWEERAIAQREHDERANAQREAEANLRAAQAALQTAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLGYTQVRAPVAGRVGRIEVTVGNLVAAGAGAPVLTTLVSVSPIYASFDTDEQVVARAL
CCCCHHHHCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHCCEEEECCCCHHHHHHHH
EDLRTSTRSSGNARAAVDRIPVQMGTGTTGGTPHVGHLQLIDNQVDARSGTVRVRAVFDN
HHHHHHHCCCCCCEEHHHHCCEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEC
ADGTLMPGQFARIRMGQAHRSSAVLINERAVGTDQNKKFVMVAGEGDKAEYREVHLGVHV
CCCCCCCCCHHEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEE
DGLRVVTSGLKAGERIVVNGLQRVRPGVQVAPQEVPMAAKAELDGNGAQARATPKQPAA
CHHHHHHHHHCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]