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The map label for this gene is hisF [H]

Identifier: 222109953

GI number: 222109953

Start: 770023

End: 770802

Strand: Direct

Name: hisF [H]

Synonym: Dtpsy_0738

Alternate gene names: 222109953

Gene position: 770023-770802 (Clockwise)

Preceding gene: 222109952

Following gene: 222109954

Centisome position: 20.28

GC content: 68.72

Gene sequence:

>780_bases
ATGCTTGCCAAACGCATCATTCCCTGCCTTGACGTGACCGGTGGCCGTGTCGTCAAGGGTGTCAACTTTGTCGAGCTGCG
CGACGCGGGCGATCCGGTGGAGATCGCCGCGCGCTACAACGCGCAGGGCGCCGACGAGCTCACCTTCCTGGACATCACCG
CCACCAGCGACGAGCGCGACCTGATCCTGCCCATCATCGAGGCCGTGGCCAGCCAGGTTTTCATCCCCTTGACCGTAGGT
GGCGGCGTGCGCACCGTGGAAGACGTGCGCCGCCTGCTCAATGCGGGCGCGGACAAGACCAGCTTCAACTCCGCCGCGAT
CGCCAACCCCGACGTCATCAGCCAGGCCTCGGCCAAGTACGGCGCGCAGTGCATCGTCGTGGCCATCGATGCCAAGCGCC
GCCAGGGCGCGGAAGTAGCCGAGCGCGGCGAGGGCTGGGATGTGTACAGCCATGGCGGGCGCAAGAACACCGGCCTGGAC
GCCGTGCAGTGGGCCGTGGAGATGGCGCGCCGCGGTGCGGGCGAGATCCTGCTGACCAGCATGGACCGCGACGGCACCAA
GAGCGGGTTTGATCTGCAACTCACCCGTGCGGTGAGCGACGCCGTGGGCGTGCCCGTGATCGCCTCGGGCGGCGTGGGCA
ACCTGGACCATCTGGCCGATGGGGTGCAGCAGGGCGGCGCGGATGCGGTGCTGGCGGCCAGCATCTTCCACTACGGCGAG
TTCACCGTGCGCCAGGCCAAGGAGCGCATGCGCGAGCGCGGCATCCCCGTGCGGCTCTGA

Upstream 100 bases:

>100_bases
AAATCCGACGAGCGCGAAAAGCTATTGATTCAATAGCTGAGCATGGTGCCGCCCATGGTATCTTGCGGCCCTTCTTCTTC
AAAAGCCGGAGTGCTGGCGC

Downstream 100 bases:

>100_bases
CGCCGTGAATGGATGCCGAAAAGGCCTGTAGCGCGCTTCCAGCAAGCGCTATAAGCTATAAAAAATGCAGCATACATCGG
CGTTGTAGCTGCCGATGACC

Product: imidazole glycerol phosphate synthase subunit hisf

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNAQGADELTFLDITATSDERDLILPIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAAIANPDVISQASAKYGAQCIVVAIDAKRRQGAEVAERGEGWDVYSHGGRKNTGLD
AVQWAVEMARRGAGEILLTSMDRDGTKSGFDLQLTRAVSDAVGVPVIASGGVGNLDHLADGVQQGGADAVLAASIFHYGE
FTVRQAKERMRERGIPVRL

Sequences:

>Translated_259_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNAQGADELTFLDITATSDERDLILPIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAAIANPDVISQASAKYGAQCIVVAIDAKRRQGAEVAERGEGWDVYSHGGRKNTGLD
AVQWAVEMARRGAGEILLTSMDRDGTKSGFDLQLTRAVSDAVGVPVIASGGVGNLDHLADGVQQGGADAVLAASIFHYGE
FTVRQAKERMRERGIPVRL
>Mature_259_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNAQGADELTFLDITATSDERDLILPIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAAIANPDVISQASAKYGAQCIVVAIDAKRRQGAEVAERGEGWDVYSHGGRKNTGLD
AVQWAVEMARRGAGEILLTSMDRDGTKSGFDLQLTRAVSDAVGVPVIASGGVGNLDHLADGVQQGGADAVLAASIFHYGE
FTVRQAKERMRERGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=263, Percent_Identity=41.06463878327, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=31.8367346938775, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.031847133758, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27456; Mature: 27456

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNAQGADELTFLDITATSDERD
CCHHHCCCHHCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSFNSAAIANPDVISQASAKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCC
GAQCIVVAIDAKRRQGAEVAERGEGWDVYSHGGRKNTGLDAVQWAVEMARRGAGEILLTS
CCEEEEEEEECCCCCCCHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
MDRDGTKSGFDLQLTRAVSDAVGVPVIASGGVGNLDHLADGVQQGGADAVLAASIFHYGE
CCCCCCCCCCCEEEHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
FTVRQAKERMRERGIPVRL
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNAQGADELTFLDITATSDERD
CCHHHCCCHHCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSFNSAAIANPDVISQASAKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCC
GAQCIVVAIDAKRRQGAEVAERGEGWDVYSHGGRKNTGLDAVQWAVEMARRGAGEILLTS
CCEEEEEEEECCCCCCCHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
MDRDGTKSGFDLQLTRAVSDAVGVPVIASGGVGNLDHLADGVQQGGADAVLAASIFHYGE
CCCCCCCCCCCEEEHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
FTVRQAKERMRERGIPVRL
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA