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The map label for this gene is hisH [H]

Identifier: 222109946

GI number: 222109946

Start: 761187

End: 761855

Strand: Direct

Name: hisH [H]

Synonym: Dtpsy_0731

Alternate gene names: 222109946

Gene position: 761187-761855 (Clockwise)

Preceding gene: 222109945

Following gene: 222109947

Centisome position: 20.05

GC content: 68.16

Gene sequence:

>669_bases
ATGAATAGTGAAGCAAAAACCGTCGCGGTCGTGGACTACGGCATGGGCAACCTGCGCTCCGTCTCGCAGGCCGTGCAGGT
CGCGGCGCAGGGCAGCGGTTTCCGGGTAATGGTCACCGCGCACCCCGATGAGGTGCGCGCGGCCGACCGTGTGGTGTTGC
CCGGCCAGGGCGCCATGCCGGACTGCATGCGCGAGTTGCGGGAGTCGGGCCTGCTCGAATCCGTGCTGGAGGCTGCCGCA
TCCAAGCCGCTGTTTGGCGTATGTGTGGGCATGCAGATGCTGCTGGACCACAGCGCCGAGGGCGACACCCCCGGGCTGGG
CCTGATCCCGGGCGAGGTGCTCAAGTTCGACCTGGCCGGGCGCACCCAGCCCGACGGCAGCCGCTACAAGGTCCCCCAGA
TGGGCTGGAACCGCGTGCGCCAGGCGCGCCACGGCGGCGTGGCGCACCCGGTATGGGCGGGCGTGCCCGACGACAGCTAC
TTCTACTTCGTGCACAGCTTCTACGCGCGCCCGGCCAACCCGCTGCACTGCGCCGGCGAGGCTGACTACGGCGGCCCCTT
TGCTGCGGCCATCGCACGCGATAATATTTTTGCTACCCAGTTCCACCCGGAGAAAAGCGCCGAGCACGGCCTGGCGCTGT
ACCGCAACTTCTTGCACTGGAAGCCCTGA

Upstream 100 bases:

>100_bases
GCGACCCGCGCGCCGCAGGCGTGATCCCCTCCACCAAGGGTTCCCTGTAAAATTCAAGCCAAATTGGCCTGTAACGCTTA
TCCATCAAGCGCAAGCAGCT

Downstream 100 bases:

>100_bases
TTCTTCAACCCGGCAGGGCCGGGCCTTGTGCGGCTCGCCCCATTCCCCGCTCACCATCATGCTGCTCATCCCCGCCATCG
ACCTCAAGGACGGCCACTGC

Product: imidazole glycerol phosphate synthase subunit hish

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNSEAKTVAVVDYGMGNLRSVSQAVQVAAQGSGFRVMVTAHPDEVRAADRVVLPGQGAMPDCMRELRESGLLESVLEAAA
SKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGEVLKFDLAGRTQPDGSRYKVPQMGWNRVRQARHGGVAHPVWAGVPDDSY
FYFVHSFYARPANPLHCAGEADYGGPFAAAIARDNIFATQFHPEKSAEHGLALYRNFLHWKP

Sequences:

>Translated_222_residues
MNSEAKTVAVVDYGMGNLRSVSQAVQVAAQGSGFRVMVTAHPDEVRAADRVVLPGQGAMPDCMRELRESGLLESVLEAAA
SKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGEVLKFDLAGRTQPDGSRYKVPQMGWNRVRQARHGGVAHPVWAGVPDDSY
FYFVHSFYARPANPLHCAGEADYGGPFAAAIARDNIFATQFHPEKSAEHGLALYRNFLHWKP
>Mature_222_residues
MNSEAKTVAVVDYGMGNLRSVSQAVQVAAQGSGFRVMVTAHPDEVRAADRVVLPGQGAMPDCMRELRESGLLESVLEAAA
SKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGEVLKFDLAGRTQPDGSRYKVPQMGWNRVRQARHGGVAHPVWAGVPDDSY
FYFVHSFYARPANPLHCAGEADYGGPFAAAIARDNIFATQFHPEKSAEHGLALYRNFLHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=214, Percent_Identity=37.8504672897196, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=225, Percent_Identity=32.8888888888889, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 24034; Mature: 24034

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSEAKTVAVVDYGMGNLRSVSQAVQVAAQGSGFRVMVTAHPDEVRAADRVVLPGQGAMP
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCEEEECCCCCCH
DCMRELRESGLLESVLEAAASKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGEVLKFDLAG
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCC
RTQPDGSRYKVPQMGWNRVRQARHGGVAHPVWAGVPDDSYFYFVHSFYARPANPLHCAGE
CCCCCCCEEECCCCCHHHHHHHHCCCCCCCEECCCCCCCEEHEEEHHHCCCCCCEEECCC
ADYGGPFAAAIARDNIFATQFHPEKSAEHGLALYRNFLHWKP
CCCCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSEAKTVAVVDYGMGNLRSVSQAVQVAAQGSGFRVMVTAHPDEVRAADRVVLPGQGAMP
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCEEEECCCCCCH
DCMRELRESGLLESVLEAAASKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGEVLKFDLAG
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCC
RTQPDGSRYKVPQMGWNRVRQARHGGVAHPVWAGVPDDSYFYFVHSFYARPANPLHCAGE
CCCCCCCEEECCCCCHHHHHHHHCCCCCCCEECCCCCCCEEHEEEHHHCCCCCCEEECCC
ADYGGPFAAAIARDNIFATQFHPEKSAEHGLALYRNFLHWKP
CCCCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]