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The map label for this gene is gsiB [H]

Identifier: 222109882

GI number: 222109882

Start: 690685

End: 692256

Strand: Direct

Name: gsiB [H]

Synonym: Dtpsy_0666

Alternate gene names: 222109882

Gene position: 690685-692256 (Clockwise)

Preceding gene: 222109881

Following gene: 222109883

Centisome position: 18.19

GC content: 68.51

Gene sequence:

>1572_bases
ATGCGAAATATGGCCCTGGCGCTTGTACTGCAAGCGCTAGCAGCTATATTTTTGGTAGCTAATGCGCAAACCATCCGTGT
GGCCAACCAGGGCGATGCGCTGTCGATGGACCCACATGCGCTCAATGAATCGCTGCAGCTCAGCCTGACCGGCAATGTGT
ATGAGCCTCTGGTGGGACGCAACAAGGATCTGAGCCTGGTGCCTGCGCTGGCGCTGGCCTGGCGTGCGACCACCCCCACC
GTGTGGCGCTTCGAGTTGCGCCGCGGCGTGTCCTTCCACGACGGTGCGCTTTTCACGGCCGACGATGCGGTGTTTTCGCT
GCGGCGCGCGCAGTCCGAAGGCTCGGACATGCGCAGTTACCTGAGCGGCGTGCGCGAGGTGCGCAAGTTGGACGCGCACA
CCATCGAGATCGAAACCCGCGAGCCCACGCCGCTGCTGCCGGCCCTGCTGTCGCACGTCTACATGATGAACCAGCGCTGG
AGCGAGGCGCAGGGTCTCGCGCAGGTAGGCGACGCGCGCGGGGCCGCGGGGCAGGCCGCCGCGCTGCGCGCCAATGGCAC
GGGGCCGTTCCGCCTGGCCGAGCGTCGCCCGCAGCAGCGCACCGTGTTCGAGCGCAACATGCGCTACTGGGGCACTATCG
AGAGCAACGCACGCCAAGTCGTCTTCCTGCCCATTCCGGACAACGAGGCGCGCGTGGCCGCGCTGCTGGCCGGCCGCGTG
GACGTGATGGAGCCCGTGCCGGTACAGGACATCGAACGCGTGAGCGCCGCCGGACTGGTCCGCGTGGTCACGGGGCCCGA
GTTGCGCACGCTGTTCCTGGGCATGGACCAGCACAGCGACGAGCTGCCGTACGCCAGCGTGCGCGGCGCCAACCCCTTCA
AGGACCGGCGCGTACGCCAGGCCTTCTACCAGGCCATCGACATCGATGCGCTCATCAAGAACGTGATGCGCGGCGCCGCC
ACGCCCGCCGCGCTCATCGTGGGACCGGGCGTCAATGGCTTTCAGTCCGACGTCAAGCGGCTGCCGCACGACGTGGCGGC
CGCGCGCGCGCTCATGGCGCAGGCCGGCTATGGCGAGGGCTTCGCGCTCACGCTGGACTGCCCCAGCGACCGCTATGTGA
ACGACGCGGCGCTGTGCACCGCCATCGCTGCACAGCTGGCGACCCTGCAGGTGCGCGTGACGGTGCGCGCGGAGCCCAAG
GCGCAGTACTACCCGCGCATCCTGCGACGCGACGCGGGCTTCTACCTGATGGGCTGGACGCCCTCCACCTACGACGCACA
CGGTGCGCTGAACGCGCTGGCGGCCTGCCCGCGCGGCGAAGGCGCGGGCCACTTCAACCTGGGGGGCTATTGCAACCCGC
GGCTGGACGCGCTGCTGCTGCAGGTCCAGACGCAGACCGACAAGACCCGGCGCGATGTGCTGCTGCGCGAGGCTTTGCTG
CTGCAGGCCGCCGACATTGCCTACATCCCGCTGCACCAGCAGGCCCTGGCCTGGGGCGTGTCCAAGAAGATCCGCCTGGT
GCAGATGGCCGACAACACCATGCCTTTCAAGTGGATGGGCGTGGGCCCCTGA

Upstream 100 bases:

>100_bases
GGCGCGGCCCTGCATGCCGGCCTGGTCGAGCAGGCCATGCCCCTGGGGCAAGCGCAGTGAGATCGCACGGTACAGCAGCA
TGATGCGGAGCACGGTCTGG

Downstream 100 bases:

>100_bases
CCGTACGACCTTGCCGCCTTCCCCTTTTTTGCCTTGACCCTTCGCTCATGAAACAAACGCTCTTGCGCTGGTACGACAGC
GACGTGGCCCACAGCTTTCG

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MRNMALALVLQALAAIFLVANAQTIRVANQGDALSMDPHALNESLQLSLTGNVYEPLVGRNKDLSLVPALALAWRATTPT
VWRFELRRGVSFHDGALFTADDAVFSLRRAQSEGSDMRSYLSGVREVRKLDAHTIEIETREPTPLLPALLSHVYMMNQRW
SEAQGLAQVGDARGAAGQAAALRANGTGPFRLAERRPQQRTVFERNMRYWGTIESNARQVVFLPIPDNEARVAALLAGRV
DVMEPVPVQDIERVSAAGLVRVVTGPELRTLFLGMDQHSDELPYASVRGANPFKDRRVRQAFYQAIDIDALIKNVMRGAA
TPAALIVGPGVNGFQSDVKRLPHDVAAARALMAQAGYGEGFALTLDCPSDRYVNDAALCTAIAAQLATLQVRVTVRAEPK
AQYYPRILRRDAGFYLMGWTPSTYDAHGALNALAACPRGEGAGHFNLGGYCNPRLDALLLQVQTQTDKTRRDVLLREALL
LQAADIAYIPLHQQALAWGVSKKIRLVQMADNTMPFKWMGVGP

Sequences:

>Translated_523_residues
MRNMALALVLQALAAIFLVANAQTIRVANQGDALSMDPHALNESLQLSLTGNVYEPLVGRNKDLSLVPALALAWRATTPT
VWRFELRRGVSFHDGALFTADDAVFSLRRAQSEGSDMRSYLSGVREVRKLDAHTIEIETREPTPLLPALLSHVYMMNQRW
SEAQGLAQVGDARGAAGQAAALRANGTGPFRLAERRPQQRTVFERNMRYWGTIESNARQVVFLPIPDNEARVAALLAGRV
DVMEPVPVQDIERVSAAGLVRVVTGPELRTLFLGMDQHSDELPYASVRGANPFKDRRVRQAFYQAIDIDALIKNVMRGAA
TPAALIVGPGVNGFQSDVKRLPHDVAAARALMAQAGYGEGFALTLDCPSDRYVNDAALCTAIAAQLATLQVRVTVRAEPK
AQYYPRILRRDAGFYLMGWTPSTYDAHGALNALAACPRGEGAGHFNLGGYCNPRLDALLLQVQTQTDKTRRDVLLREALL
LQAADIAYIPLHQQALAWGVSKKIRLVQMADNTMPFKWMGVGP
>Mature_523_residues
MRNMALALVLQALAAIFLVANAQTIRVANQGDALSMDPHALNESLQLSLTGNVYEPLVGRNKDLSLVPALALAWRATTPT
VWRFELRRGVSFHDGALFTADDAVFSLRRAQSEGSDMRSYLSGVREVRKLDAHTIEIETREPTPLLPALLSHVYMMNQRW
SEAQGLAQVGDARGAAGQAAALRANGTGPFRLAERRPQQRTVFERNMRYWGTIESNARQVVFLPIPDNEARVAALLAGRV
DVMEPVPVQDIERVSAAGLVRVVTGPELRTLFLGMDQHSDELPYASVRGANPFKDRRVRQAFYQAIDIDALIKNVMRGAA
TPAALIVGPGVNGFQSDVKRLPHDVAAARALMAQAGYGEGFALTLDCPSDRYVNDAALCTAIAAQLATLQVRVTVRAEPK
AQYYPRILRRDAGFYLMGWTPSTYDAHGALNALAACPRGEGAGHFNLGGYCNPRLDALLLQVQTQTDKTRRDVLLREALL
LQAADIAYIPLHQQALAWGVSKKIRLVQMADNTMPFKWMGVGP

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=535, Percent_Identity=26.1682242990654, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787052, Length=492, Percent_Identity=23.9837398373984, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789397, Length=509, Percent_Identity=24.75442043222, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=22.6591760299625, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081878, Length=491, Percent_Identity=24.6435845213849, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789887, Length=483, Percent_Identity=23.3954451345756, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787762, Length=517, Percent_Identity=22.8239845261122, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787551, Length=451, Percent_Identity=25.2771618625277, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57283; Mature: 57283

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNMALALVLQALAAIFLVANAQTIRVANQGDALSMDPHALNESLQLSLTGNVYEPLVGR
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCCHHHHCCC
NKDLSLVPALALAWRATTPTVWRFELRRGVSFHDGALFTADDAVFSLRRAQSEGSDMRSY
CCCCHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHH
LSGVREVRKLDAHTIEIETREPTPLLPALLSHVYMMNQRWSEAQGLAQVGDARGAAGQAA
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEE
ALRANGTGPFRLAERRPQQRTVFERNMRYWGTIESNARQVVFLPIPDNEARVAALLAGRV
EEEECCCCCCHHHHCCCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCC
DVMEPVPVQDIERVSAAGLVRVVTGPELRTLFLGMDQHSDELPYASVRGANPFKDRRVRQ
CCCCCCCHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
AFYQAIDIDALIKNVMRGAATPAALIVGPGVNGFQSDVKRLPHDVAAARALMAQAGYGEG
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FALTLDCPSDRYVNDAALCTAIAAQLATLQVRVTVRAEPKAQYYPRILRRDAGFYLMGWT
EEEEEECCCCCCCCHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHCCCCEEEECCC
PSTYDAHGALNALAACPRGEGAGHFNLGGYCNPRLDALLLQVQTQTDKTRRDVLLREALL
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHH
LQAADIAYIPLHQQALAWGVSKKIRLVQMADNTMPFKWMGVGP
HHHCCEEEECCHHHHHHHCCCCCEEEEEEECCCCCEEEEECCC
>Mature Secondary Structure
MRNMALALVLQALAAIFLVANAQTIRVANQGDALSMDPHALNESLQLSLTGNVYEPLVGR
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCCHHHHCCC
NKDLSLVPALALAWRATTPTVWRFELRRGVSFHDGALFTADDAVFSLRRAQSEGSDMRSY
CCCCHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHH
LSGVREVRKLDAHTIEIETREPTPLLPALLSHVYMMNQRWSEAQGLAQVGDARGAAGQAA
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEE
ALRANGTGPFRLAERRPQQRTVFERNMRYWGTIESNARQVVFLPIPDNEARVAALLAGRV
EEEECCCCCCHHHHCCCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCC
DVMEPVPVQDIERVSAAGLVRVVTGPELRTLFLGMDQHSDELPYASVRGANPFKDRRVRQ
CCCCCCCHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
AFYQAIDIDALIKNVMRGAATPAALIVGPGVNGFQSDVKRLPHDVAAARALMAQAGYGEG
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FALTLDCPSDRYVNDAALCTAIAAQLATLQVRVTVRAEPKAQYYPRILRRDAGFYLMGWT
EEEEEECCCCCCCCHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHCCCCEEEECCC
PSTYDAHGALNALAACPRGEGAGHFNLGGYCNPRLDALLLQVQTQTDKTRRDVLLREALL
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHH
LQAADIAYIPLHQQALAWGVSKKIRLVQMADNTMPFKWMGVGP
HHHCCEEEECCHHHHHHHCCCCCEEEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA