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The map label for this gene is glyS

Identifier: 222109760

GI number: 222109760

Start: 563042

End: 565204

Strand: Direct

Name: glyS

Synonym: Dtpsy_0544

Alternate gene names: 222109760

Gene position: 563042-565204 (Clockwise)

Preceding gene: 222109759

Following gene: 222109761

Centisome position: 14.83

GC content: 67.59

Gene sequence:

>2163_bases
ATGAATCACCAGAATCTTCTTGTCGAACTGTTTGTCGAAGAACTGCCCCCGAAGGCCCTGCAAAAGCTCGGCGATGCCTT
CGCGGGCGTGCTGCTGGAGCAATTGCAGGCGCAAGGCCTGACCTCGGCACATTCGCAGCTCACCGCCTTTGCCTCGCCGC
GCCGGCTGGCGGCGCACATCACCGAAGTGCTGCCCGCCGCGGCCGACAAGGCGGTGTCGCAAAAGCTCATGCCCGTGGCC
GTGGGTCTGGATGCGAGCGGCCAGCCCACGCCGGCGCTGCTCAAGAAGTTGGCTGCGCTGGGCGCCGACGCCGCCAGCGT
GCCGCAGCTCAAGCGCGTGCACGATGGCAAGGCCGAGGTGCTGTTCTTTGAAAGCATGGCCAAGGGCGCGCTGCTGGCCG
ATGGCCTGCAGAAGGCGCTGGACGAGGCCATTGCCAAGCTGCCGATCCCGAAGGTGATGCGCTACCAGTTGCAGGACGGT
TGGACCAGCGTGCATTTCGTGCGCCCGGCGCACGGCCTGGTGGCGCTGCATGGCAGCGAGGTGCTGGTGGGCGTGCAGGC
CCTGGGCCTGACGGCCGGCAACACCACGCACGGCCACCGCTTCGAGGCCTCCGTGGACCCGGTCGTGATCCAGAGCGCCG
ACAGCTACGCCGAGCAACTGCGCAGCGAAGGCGCGGTGATTGCTAGCTTTGCCGAGCGCCGCGCCGAGATCGCGCGCCAG
CTGCAGGCGGCCGCCGACCGTGTAGGCGGCGGTGTGCGCCCCATCGACGACGATGCGTTACTCGACGAGGTGACGGCGCT
GGTCGAGCGTCCGAACGTGCTGGTCTGCGAATTCGAGAAGGACTTCCTCGCCGTGCCGCAGGAGTGCCTGATCCTCACCA
TGAAGGCCAACCAGAAGTACTTCCCGCTGCTGGATGCCGAGGGCAAGCTCACCCACCAGTTCCTGGTGGTGAGCAACATC
AGCCCGCAGGACGCCAGCGCCGTCATCCAGGGCAACGAGCGCGTGGTGCGCCCGCGCCTGGCGGATGCCAAGTTCTTCTT
CGATCAGGACCGCAAGAAGACCCTGGTCAGCCGCGTGGACCAGCTGGCCAAGGTGGTCTACCACAACAAGCTGGGCACGC
AGGGCGAACGCGTGGAGCGGGTGCGCCATATCGCCAAGGCCATCGCCACGCAGCTGTTCACGGCGCTTGCGCAAGGCAAC
GCGGCGCTGGACTCTCAGGAAGGCGAGATCGCGCAGGACTATCTGCTGACCTGCGTGGACAACGCCGCGCTGCTGGCCAA
GACCGACCTGGTCACCGACATGGTGGGCGAGTTCCCCGAGCTGCAGGGCATCATGGGCGGCTACTACGCGGTGAGCGACG
GCCTGCCCGACGAGGTGGCGCATGCCATCGAGGACCATTACAAGCCGCGCTTTGCCGGCGACGCGCTGCCGCGCGAGAAC
GTGGGCGTGGTCGTTGCGCTGGCCGACAAGCTGGAGACGCTGGTGGGCATGTTCGGCATTGGCAACCTGCCCACGGGCGA
CCGCGACCCGTTTGCGCTGCGCCGCCATGCGCTGGGCGTGATCCGCATGCTGGTCGAGAAGGAGCTGCCGCTGGATCTGG
ATGCGCTGCTGGCCAGCGCGGTGCCGGCTTTCGGCGACAAGATCGAGGACACCAGCGCCCAGTTGGCCGACTTCATCTAC
GACCGCCTGGCTGGCTCCCTGCGCGAGCAGGGCTACAGCGCCCAGGAGGTGGACGCCGTGATTGCCCTGCGTCCGCAGCG
CCTGGCGCTGGTGCCCCGGCAGATCGAGGCCGTGCGTGCATTCGCCACGCTGGAGCAGGCGCCCGCGCTGGCCGCGGCCA
ACAAGCGCGTGACCAACATCCTCAAGAAGGCCGGCGAAGTGGATCCGCACGTGAATGAGGAACTGCTGCAAGAGCCCGCC
GAGAAGGACCTGTACGCCGCGCTGCAGCGCTTCGTGCCCGAGGCCAACGCCCAGTTCGACAGCGGCGACTACACCGCCAG
CCTGCAAACCCTGGCCGTGCTGCGCGCGCCCGTGGACGCGTTCTTCGACGACGTGATGGTCAATGCCGAGGAGCTGGCGT
TGCGCCTGAACCGCCAGGGCCTGCTCAAGAAGCTGCACATGGCGATGAACCGCGTGGCCGACCTCTCGCGCCTGGCGGTG
TGA

Upstream 100 bases:

>100_bases
GGCGCATGCCGACGAAGTGCTGGCCGAACTGGCCAAGGCCGCGGAACAACAGAACAAGAAGGCCGCCTGAGCGCACGCGC
CGCGCAGCGAGAAACACACC

Downstream 100 bases:

>100_bases
CCGTGCGCGGCTGTGGGGCCTGGACCGTTACGCCGGTGACGGCGGACGCGGGCGGCGCGGCAGCGTTGCTTTGATAATCG
CGCCATGAAACTCGCCATCC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MNHQNLLVELFVEELPPKALQKLGDAFAGVLLEQLQAQGLTSAHSQLTAFASPRRLAAHITEVLPAAADKAVSQKLMPVA
VGLDASGQPTPALLKKLAALGADAASVPQLKRVHDGKAEVLFFESMAKGALLADGLQKALDEAIAKLPIPKVMRYQLQDG
WTSVHFVRPAHGLVALHGSEVLVGVQALGLTAGNTTHGHRFEASVDPVVIQSADSYAEQLRSEGAVIASFAERRAEIARQ
LQAAADRVGGGVRPIDDDALLDEVTALVERPNVLVCEFEKDFLAVPQECLILTMKANQKYFPLLDAEGKLTHQFLVVSNI
SPQDASAVIQGNERVVRPRLADAKFFFDQDRKKTLVSRVDQLAKVVYHNKLGTQGERVERVRHIAKAIATQLFTALAQGN
AALDSQEGEIAQDYLLTCVDNAALLAKTDLVTDMVGEFPELQGIMGGYYAVSDGLPDEVAHAIEDHYKPRFAGDALPREN
VGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLVEKELPLDLDALLASAVPAFGDKIEDTSAQLADFIY
DRLAGSLREQGYSAQEVDAVIALRPQRLALVPRQIEAVRAFATLEQAPALAAANKRVTNILKKAGEVDPHVNEELLQEPA
EKDLYAALQRFVPEANAQFDSGDYTASLQTLAVLRAPVDAFFDDVMVNAEELALRLNRQGLLKKLHMAMNRVADLSRLAV

Sequences:

>Translated_720_residues
MNHQNLLVELFVEELPPKALQKLGDAFAGVLLEQLQAQGLTSAHSQLTAFASPRRLAAHITEVLPAAADKAVSQKLMPVA
VGLDASGQPTPALLKKLAALGADAASVPQLKRVHDGKAEVLFFESMAKGALLADGLQKALDEAIAKLPIPKVMRYQLQDG
WTSVHFVRPAHGLVALHGSEVLVGVQALGLTAGNTTHGHRFEASVDPVVIQSADSYAEQLRSEGAVIASFAERRAEIARQ
LQAAADRVGGGVRPIDDDALLDEVTALVERPNVLVCEFEKDFLAVPQECLILTMKANQKYFPLLDAEGKLTHQFLVVSNI
SPQDASAVIQGNERVVRPRLADAKFFFDQDRKKTLVSRVDQLAKVVYHNKLGTQGERVERVRHIAKAIATQLFTALAQGN
AALDSQEGEIAQDYLLTCVDNAALLAKTDLVTDMVGEFPELQGIMGGYYAVSDGLPDEVAHAIEDHYKPRFAGDALPREN
VGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLVEKELPLDLDALLASAVPAFGDKIEDTSAQLADFIY
DRLAGSLREQGYSAQEVDAVIALRPQRLALVPRQIEAVRAFATLEQAPALAAANKRVTNILKKAGEVDPHVNEELLQEPA
EKDLYAALQRFVPEANAQFDSGDYTASLQTLAVLRAPVDAFFDDVMVNAEELALRLNRQGLLKKLHMAMNRVADLSRLAV
>Mature_720_residues
MNHQNLLVELFVEELPPKALQKLGDAFAGVLLEQLQAQGLTSAHSQLTAFASPRRLAAHITEVLPAAADKAVSQKLMPVA
VGLDASGQPTPALLKKLAALGADAASVPQLKRVHDGKAEVLFFESMAKGALLADGLQKALDEAIAKLPIPKVMRYQLQDG
WTSVHFVRPAHGLVALHGSEVLVGVQALGLTAGNTTHGHRFEASVDPVVIQSADSYAEQLRSEGAVIASFAERRAEIARQ
LQAAADRVGGGVRPIDDDALLDEVTALVERPNVLVCEFEKDFLAVPQECLILTMKANQKYFPLLDAEGKLTHQFLVVSNI
SPQDASAVIQGNERVVRPRLADAKFFFDQDRKKTLVSRVDQLAKVVYHNKLGTQGERVERVRHIAKAIATQLFTALAQGN
AALDSQEGEIAQDYLLTCVDNAALLAKTDLVTDMVGEFPELQGIMGGYYAVSDGLPDEVAHAIEDHYKPRFAGDALPREN
VGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLVEKELPLDLDALLASAVPAFGDKIEDTSAQLADFIY
DRLAGSLREQGYSAQEVDAVIALRPQRLALVPRQIEAVRAFATLEQAPALAAANKRVTNILKKAGEVDPHVNEELLQEPA
EKDLYAALQRFVPEANAQFDSGDYTASLQTLAVLRAPVDAFFDDVMVNAEELALRLNRQGLLKKLHMAMNRVADLSRLAV

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=723, Percent_Identity=41.6320885200553, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_ACIET (B9MCM4)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552024.1
- GeneID:   7382835
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_0544
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 77924; Mature: 77924

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQNLLVELFVEELPPKALQKLGDAFAGVLLEQLQAQGLTSAHSQLTAFASPRRLAAHI
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
TEVLPAAADKAVSQKLMPVAVGLDASGQPTPALLKKLAALGADAASVPQLKRVHDGKAEV
HHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCEE
LFFESMAKGALLADGLQKALDEAIAKLPIPKVMRYQLQDGWTSVHFVRPAHGLVALHGSE
EEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCCH
VLVGVQALGLTAGNTTHGHRFEASVDPVVIQSADSYAEQLRSEGAVIASFAERRAEIARQ
HEEEHHHHEEECCCCCCCCEEECCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LQAAADRVGGGVRPIDDDALLDEVTALVERPNVLVCEFEKDFLAVPQECLILTMKANQKY
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHCCHHHEEEEEECCCCC
FPLLDAEGKLTHQFLVVSNISPQDASAVIQGNERVVRPRLADAKFFFDQDRKKTLVSRVD
CCEECCCCCCEEEEEEEECCCCCCCHHEEECCCEEECCCCCCHHHHHCCHHHHHHHHHHH
QLAKVVYHNKLGTQGERVERVRHIAKAIATQLFTALAQGNAALDSQEGEIAQDYLLTCVD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
NAALLAKTDLVTDMVGEFPELQGIMGGYYAVSDGLPDEVAHAIEDHYKPRFAGDALPREN
CCHHHHHHHHHHHHHHCCHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
VGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLVEKELPLDLDALLASA
CCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VPAFGDKIEDTSAQLADFIYDRLAGSLREQGYSAQEVDAVIALRPQRLALVPRQIEAVRA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHHHHHHH
FATLEQAPALAAANKRVTNILKKAGEVDPHVNEELLQEPAEKDLYAALQRFVPEANAQFD
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCC
SGDYTASLQTLAVLRAPVDAFFDDVMVNAEELALRLNRQGLLKKLHMAMNRVADLSRLAV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHQNLLVELFVEELPPKALQKLGDAFAGVLLEQLQAQGLTSAHSQLTAFASPRRLAAHI
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
TEVLPAAADKAVSQKLMPVAVGLDASGQPTPALLKKLAALGADAASVPQLKRVHDGKAEV
HHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCEE
LFFESMAKGALLADGLQKALDEAIAKLPIPKVMRYQLQDGWTSVHFVRPAHGLVALHGSE
EEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCCH
VLVGVQALGLTAGNTTHGHRFEASVDPVVIQSADSYAEQLRSEGAVIASFAERRAEIARQ
HEEEHHHHEEECCCCCCCCEEECCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LQAAADRVGGGVRPIDDDALLDEVTALVERPNVLVCEFEKDFLAVPQECLILTMKANQKY
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHCCHHHEEEEEECCCCC
FPLLDAEGKLTHQFLVVSNISPQDASAVIQGNERVVRPRLADAKFFFDQDRKKTLVSRVD
CCEECCCCCCEEEEEEEECCCCCCCHHEEECCCEEECCCCCCHHHHHCCHHHHHHHHHHH
QLAKVVYHNKLGTQGERVERVRHIAKAIATQLFTALAQGNAALDSQEGEIAQDYLLTCVD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
NAALLAKTDLVTDMVGEFPELQGIMGGYYAVSDGLPDEVAHAIEDHYKPRFAGDALPREN
CCHHHHHHHHHHHHHHCCHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
VGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLVEKELPLDLDALLASA
CCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VPAFGDKIEDTSAQLADFIYDRLAGSLREQGYSAQEVDAVIALRPQRLALVPRQIEAVRA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHHHHHHH
FATLEQAPALAAANKRVTNILKKAGEVDPHVNEELLQEPAEKDLYAALQRFVPEANAQFD
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCC
SGDYTASLQTLAVLRAPVDAFFDDVMVNAEELALRLNRQGLLKKLHMAMNRVADLSRLAV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA