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The map label for this gene is iciA [H]

Identifier: 222109314

GI number: 222109314

Start: 99475

End: 100386

Strand: Direct

Name: iciA [H]

Synonym: Dtpsy_0091

Alternate gene names: 222109314

Gene position: 99475-100386 (Clockwise)

Preceding gene: 222109312

Following gene: 222109315

Centisome position: 2.62

GC content: 69.74

Gene sequence:

>912_bases
GTGAGCACATTTGACCCCGCCGCATTGGAATGCCTGGCCGCCATCGTCGAAGAGGGCGGTTTCGAGCGCGCCGCGCGCCG
CCTGAACATCACCCAGTCCGCCGTGTCGCAGCGCCTGCGTGCGCTGGAGGCGCAGGTGGGCTCGGTGCTCATCGTGCGCA
GCCGGCCGCTCAAGCCCACTTCGGCCGGGCAGTTGTTGCTCAAGCACACCAAGCAGCTGCGCCTGCTGCGCGCCGACCTG
GAGCGCGATCTGCAGGACCTCATGCCCAGCGGGCGCGGCGGCGGCCGCGAGGAAGAGCGCATCGCCATCGCCATCAACGC
CGACAGCATCGCGACCTGGGCGGTGGACGCGCTCGGCGATCTGGTGCGCCAGCGGTTGCCGCTGGAGATCATTGCCGACG
ACCAGGACTTCACGCAGGAGTGGCTGCGCTCGGGCCAGGTGATGGGCTGCGTGACGACGTTGAAGAGCGCGCTGCGCGGC
TGCCGCATGGTGCCCCTGGGCGCCATGCAGTACGTCGCCGTGGCCGCGCCGGAGCTGGCGCGCCGACAACTGCCGCAAGG
GCTCACGGCGCACAACTTCCGCGACGTGCCTTTCGTCTCGTTCAACCGCAAGGACGATATGCAGGCCGAGTTCGTCACGC
GCACCTTTGGCCTGAAACGCGTGTCTCTCAGCCGCGTGTTCGTGCCCAGCTCGGAGGGCCAGGTGCGCGCCGTGGCCGCG
GGCTGGGGCGTGAGCGTGGTGCCCGAACTGCTCGCGCGCCCCTGGCTGCAGCAAGGCCGGCTGGTGGACCTGGCGCCAGG
CCATACGCTGCCCATCCAGCTGTACTGGCACTCATGGAACCTGGAGTCGGAAGTGCTGGATGCGCTGGCCGATGCGCTCA
CCAGCGCGGCGGCGCAGTACCTGTCGGCCTGA

Upstream 100 bases:

>100_bases
CGATGGTTTTCATGGCGCGCAGTGTGCTGCGGCCCAATGAAAAACAAAAGCGTGATTAAACTTCTTCGGAATTCATCAGA
ATGACTAATGGATACCGACC

Downstream 100 bases:

>100_bases
CGGCACACGCGGATGGGGGTCTTTAGGGTTATCGGCCTGCGCGGGGTGAAGGCTCGCTAACATCGCTCGCATGAACACGT
TGCAGCCGCCCCGTCCGTCC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSTFDPAALECLAAIVEEGGFERAARRLNITQSAVSQRLRALEAQVGSVLIVRSRPLKPTSAGQLLLKHTKQLRLLRADL
ERDLQDLMPSGRGGGREEERIAIAINADSIATWAVDALGDLVRQRLPLEIIADDQDFTQEWLRSGQVMGCVTTLKSALRG
CRMVPLGAMQYVAVAAPELARRQLPQGLTAHNFRDVPFVSFNRKDDMQAEFVTRTFGLKRVSLSRVFVPSSEGQVRAVAA
GWGVSVVPELLARPWLQQGRLVDLAPGHTLPIQLYWHSWNLESEVLDALADALTSAAAQYLSA

Sequences:

>Translated_303_residues
MSTFDPAALECLAAIVEEGGFERAARRLNITQSAVSQRLRALEAQVGSVLIVRSRPLKPTSAGQLLLKHTKQLRLLRADL
ERDLQDLMPSGRGGGREEERIAIAINADSIATWAVDALGDLVRQRLPLEIIADDQDFTQEWLRSGQVMGCVTTLKSALRG
CRMVPLGAMQYVAVAAPELARRQLPQGLTAHNFRDVPFVSFNRKDDMQAEFVTRTFGLKRVSLSRVFVPSSEGQVRAVAA
GWGVSVVPELLARPWLQQGRLVDLAPGHTLPIQLYWHSWNLESEVLDALADALTSAAAQYLSA
>Mature_302_residues
STFDPAALECLAAIVEEGGFERAARRLNITQSAVSQRLRALEAQVGSVLIVRSRPLKPTSAGQLLLKHTKQLRLLRADLE
RDLQDLMPSGRGGGREEERIAIAINADSIATWAVDALGDLVRQRLPLEIIADDQDFTQEWLRSGQVMGCVTTLKSALRGC
RMVPLGAMQYVAVAAPELARRQLPQGLTAHNFRDVPFVSFNRKDDMQAEFVTRTFGLKRVSLSRVFVPSSEGQVRAVAAG
WGVSVVPELLARPWLQQGRLVDLAPGHTLPIQLYWHSWNLESEVLDALADALTSAAAQYLSA

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=302, Percent_Identity=36.4238410596026, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1786713, Length=266, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33286; Mature: 33154

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFDPAALECLAAIVEEGGFERAARRLNITQSAVSQRLRALEAQVGSVLIVRSRPLKPT
CCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
SAGQLLLKHTKQLRLLRADLERDLQDLMPSGRGGGREEERIAIAINADSIATWAVDALGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
LVRQRLPLEIIADDQDFTQEWLRSGQVMGCVTTLKSALRGCRMVPLGAMQYVAVAAPELA
HHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHH
RRQLPQGLTAHNFRDVPFVSFNRKDDMQAEFVTRTFGLKRVSLSRVFVPSSEGQVRAVAA
HHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEHEEEEECCCCCCEEEEEC
GWGVSVVPELLARPWLQQGRLVDLAPGHTLPIQLYWHSWNLESEVLDALADALTSAAAQY
CCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LSA
HCC
>Mature Secondary Structure 
STFDPAALECLAAIVEEGGFERAARRLNITQSAVSQRLRALEAQVGSVLIVRSRPLKPT
CCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
SAGQLLLKHTKQLRLLRADLERDLQDLMPSGRGGGREEERIAIAINADSIATWAVDALGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
LVRQRLPLEIIADDQDFTQEWLRSGQVMGCVTTLKSALRGCRMVPLGAMQYVAVAAPELA
HHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHH
RRQLPQGLTAHNFRDVPFVSFNRKDDMQAEFVTRTFGLKRVSLSRVFVPSSEGQVRAVAA
HHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEHEEEEECCCCCCEEEEEC
GWGVSVVPELLARPWLQQGRLVDLAPGHTLPIQLYWHSWNLESEVLDALADALTSAAAQY
CCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LSA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA