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The map label for this gene is livK [C]

Identifier: 222109312

GI number: 222109312

Start: 96844

End: 98034

Strand: Direct

Name: livK [C]

Synonym: Dtpsy_0089

Alternate gene names: 222109312

Gene position: 96844-98034 (Clockwise)

Preceding gene: 222109311

Following gene: 222109314

Centisome position: 2.55

GC content: 68.51

Gene sequence:

>1191_bases
ATGATGGCCATGAGACCCAGACACTGGGCAGGGGCGCTGGCGCTGTGGGCGTTGGCAGGCATGGCGGGCGCCCAGCCCGT
GAAGGTGGGGGTCATCGGCCCCTTTTCGGGGCCGTCGTCGGACTTCGGCACGCCCATGCTGCAGGGCATCCAGCTCGCGG
TGGAGCAGTTCAACGCCGTGGGAGGCTACCTGGGCCGACCCGTGGAGCTGGTGGTCAAGGACGACCAGGGCTCGCCCGAC
ACCGGCCGTCAACGCTCGCAGGAACTGGCCAAAGAAGGCGTGGTGGCTGCCCTGGGCTTTTGCAACACCGGCGTGGCGAT
GAAATCGCTGGACGTGTTCCAGCAGGCGAAGCTGCCCTTGATCGTGCCGTGCGCCACGGGCACCCCCGTGACACGCACCT
ATCCGGCGGCGCAGAGCTTCATCTTCCGCACCTCGGCCCCCGACAACATCCAGGCGCCCTTCGTGGTGGAGGACATCCTG
CGCCGCGGCTGGACGCGGGTCGCTCTCTTTGCCGACGACACCGGCTACGGCCAGGCCGGGCGCAAGGACGTGGAAGCGGC
GCTCGCCGCGCACCAGCTTACGCCCGTGTACGCGACCAACTTCCCTCTGGGCGTGAAGAGCCTGGCGCCCCAGTTGCAGG
CCGCGCGCGATGCGGGCGCACAGGTCATCTTCAGCTACACCGTAGGGCCGGAGAACGCTGTCATTGCCCAAGGCCGCAAG
GAGCTGGGCTGGAAGGTGCCGCAGGTCGGCGGCTGGACGCTGTCGTTCCCGTTCTTCATCCAGGGCGGCAAGGACGCCGC
CGAGGGAGCGCTGACGGCGCAGACCTTCATCGCCGAGCCCAGCAACGAACGCCGCGCCTCGTTCCTGAGCACCTACGCGC
GCGCCTTCAAGGGCCGCCCGGCCGTGCCGATGGCGGCGGCGCAAGGGTACGACGCGGCCTACCTGCTGATGTATGCGCTG
CTGGGCATCCGCGACGGGCAGGTCAACGGCCCGGCCCTCAAGCATTCGCTGGAGAACCTGCAGCGCGTGTATTACGGCGT
GGTGGCGACCTACGAGCGGCCCTTCTCGGCCGAGGAGAAAGAAGCCATCACCCCCAACATGCTGGTGATGGGCAAGGTGC
AGGCCGGCGCGGTGACCTTCGCCTACCCCGAGGATGCCAAGCGCAACCTGCTGGTGCAGCGCAAACGCTGA

Upstream 100 bases:

>100_bases
CGCCCCTGACCGCGACCGCGCACCGCTGCGCAATAAACCACATCGCGCGCCCGCGCTTTTGGTTAGGCTGGAGTGTTTTT
CACAACATTGCGGAGACACG

Downstream 100 bases:

>100_bases
GGTGCGAGCCGCTCAGGCCAGGCGCGGCGCCCCGTAGGCGGCAGCGGGCGTGTCGTCGCCCAGAAAGGCGAAGTCCATGG
CTGGCCGCTCGCCGCTCAGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MMAMRPRHWAGALALWALAGMAGAQPVKVGVIGPFSGPSSDFGTPMLQGIQLAVEQFNAVGGYLGRPVELVVKDDQGSPD
TGRQRSQELAKEGVVAALGFCNTGVAMKSLDVFQQAKLPLIVPCATGTPVTRTYPAAQSFIFRTSAPDNIQAPFVVEDIL
RRGWTRVALFADDTGYGQAGRKDVEAALAAHQLTPVYATNFPLGVKSLAPQLQAARDAGAQVIFSYTVGPENAVIAQGRK
ELGWKVPQVGGWTLSFPFFIQGGKDAAEGALTAQTFIAEPSNERRASFLSTYARAFKGRPAVPMAAAQGYDAAYLLMYAL
LGIRDGQVNGPALKHSLENLQRVYYGVVATYERPFSAEEKEAITPNMLVMGKVQAGAVTFAYPEDAKRNLLVQRKR

Sequences:

>Translated_396_residues
MMAMRPRHWAGALALWALAGMAGAQPVKVGVIGPFSGPSSDFGTPMLQGIQLAVEQFNAVGGYLGRPVELVVKDDQGSPD
TGRQRSQELAKEGVVAALGFCNTGVAMKSLDVFQQAKLPLIVPCATGTPVTRTYPAAQSFIFRTSAPDNIQAPFVVEDIL
RRGWTRVALFADDTGYGQAGRKDVEAALAAHQLTPVYATNFPLGVKSLAPQLQAARDAGAQVIFSYTVGPENAVIAQGRK
ELGWKVPQVGGWTLSFPFFIQGGKDAAEGALTAQTFIAEPSNERRASFLSTYARAFKGRPAVPMAAAQGYDAAYLLMYAL
LGIRDGQVNGPALKHSLENLQRVYYGVVATYERPFSAEEKEAITPNMLVMGKVQAGAVTFAYPEDAKRNLLVQRKR
>Mature_396_residues
MMAMRPRHWAGALALWALAGMAGAQPVKVGVIGPFSGPSSDFGTPMLQGIQLAVEQFNAVGGYLGRPVELVVKDDQGSPD
TGRQRSQELAKEGVVAALGFCNTGVAMKSLDVFQQAKLPLIVPCATGTPVTRTYPAAQSFIFRTSAPDNIQAPFVVEDIL
RRGWTRVALFADDTGYGQAGRKDVEAALAAHQLTPVYATNFPLGVKSLAPQLQAARDAGAQVIFSYTVGPENAVIAQGRK
ELGWKVPQVGGWTLSFPFFIQGGKDAAEGALTAQTFIAEPSNERRASFLSTYARAFKGRPAVPMAAAQGYDAAYLLMYAL
LGIRDGQVNGPALKHSLENLQRVYYGVVATYERPFSAEEKEAITPNMLVMGKVQAGAVTFAYPEDAKRNLLVQRKR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=301, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789867, Length=300, Percent_Identity=26, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42456; Mature: 42456

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAMRPRHWAGALALWALAGMAGAQPVKVGVIGPFSGPSSDFGTPMLQGIQLAVEQFNAV
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
GGYLGRPVELVVKDDQGSPDTGRQRSQELAKEGVVAALGFCNTGVAMKSLDVFQQAKLPL
HHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHCCCCE
IVPCATGTPVTRTYPAAQSFIFRTSAPDNIQAPFVVEDILRRGWTRVALFADDTGYGQAG
EEEECCCCCCCCCCCCHHHEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCC
RKDVEAALAAHQLTPVYATNFPLGVKSLAPQLQAARDAGAQVIFSYTVGPENAVIAQGRK
HHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCHH
ELGWKVPQVGGWTLSFPFFIQGGKDAAEGALTAQTFIAEPSNERRASFLSTYARAFKGRP
HHCCCCCCCCCEEEECCEEEECCCCHHCCCEEHHHEECCCCCHHHHHHHHHHHHHHCCCC
AVPMAAAQGYDAAYLLMYALLGIRDGQVNGPALKHSLENLQRVYYGVVATYERPFSAEEK
CCCCHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EAITPNMLVMGKVQAGAVTFAYPEDAKRNLLVQRKR
HCCCCCEEEEEEECCCEEEEECCCCCCCCCEEECCC
>Mature Secondary Structure
MMAMRPRHWAGALALWALAGMAGAQPVKVGVIGPFSGPSSDFGTPMLQGIQLAVEQFNAV
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
GGYLGRPVELVVKDDQGSPDTGRQRSQELAKEGVVAALGFCNTGVAMKSLDVFQQAKLPL
HHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHCCCCE
IVPCATGTPVTRTYPAAQSFIFRTSAPDNIQAPFVVEDILRRGWTRVALFADDTGYGQAG
EEEECCCCCCCCCCCCHHHEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCC
RKDVEAALAAHQLTPVYATNFPLGVKSLAPQLQAARDAGAQVIFSYTVGPENAVIAQGRK
HHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCHH
ELGWKVPQVGGWTLSFPFFIQGGKDAAEGALTAQTFIAEPSNERRASFLSTYARAFKGRP
HHCCCCCCCCCEEEECCEEEECCCCHHCCCEEHHHEECCCCCHHHHHHHHHHHHHHCCCC
AVPMAAAQGYDAAYLLMYALLGIRDGQVNGPALKHSLENLQRVYYGVVATYERPFSAEEK
CCCCHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EAITPNMLVMGKVQAGAVTFAYPEDAKRNLLVQRKR
HCCCCCEEEEEEECCCEEEEECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]