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The map label for this gene is dnaA [H]

Identifier: 222109226

GI number: 222109226

Start: 70

End: 1491

Strand: Direct

Name: dnaA [H]

Synonym: Dtpsy_0001

Alternate gene names: 222109226

Gene position: 70-1491 (Clockwise)

Preceding gene: NA

Following gene: 222109227

Centisome position: 0.0

GC content: 63.71

Gene sequence:

>1422_bases
ATGAACGAGGGACTTCACTCCTCCAGCCACGACAGCGACGGCGCCAGCGCCGGCCAGGGTCTGTGGCAGGCCTGCGCCGA
ACAGCTGGCGCAGGACCTCCCGGAACAGCAGTTCAACACCTGGATCAAGCCCCTGGTGGCCCAGGTCGCCCCCGATTTTT
CCAAGGTCACCCTGCTGGTGGCCAACCGCTTCAAGCTCGACTGGATCCGCGCACAGTATGCCGGCCGCATCTCGGCCCTG
CTGGAAGGTCTGTACGGCCAACCGGTCACACTGGAGTTAGCGCTTGCTCAGCGGGAATCCGTTGTCAGGACTTACGTCCG
TCCCACGGCGCCAGAGACCCCCGCCACCGGCGATGCACCGTCCGCCCACCCCACGGCCGACGAGGCGCCTGCCGGCGCCT
TCCGCACGCGCCTGAACCCGGCGTTGACCTTCGAGACGTTGGTGGAGGGCACGGCCAACCGCATGGCGCGCTCGGCGGCG
ATGCACGTGGCCGGCTCGCCCGGCCACCTGTACAACCCGCTGTTCATCTATGGCGGCGTGGGCTTGGGCAAGACCCACCT
GGTGCATGCCGTGGGCAACAAGCTGCTGCAGGACCGTCCCGACGCCAAGGTTCTCTACATCCATGCGGAGCAGTTCGTCT
CGGATGTGGTCAAGTCATACCAGCGCCGTACCTTCGACGACTTCAAGGAGCGTTACCACTCGCTCGACCTGCTGTTGATC
GACGACGTGCAATTCTTTGCCAACAAGGACCGCACGCAGGAAGAATTTTTCAACGCCTTCGAGGCGCTGCTGGCCAAGAA
GAGCCACATCGTGATGACTTCGGACACCTATCCCAAGGGTCTGGCCAACATCCACGAGCGGCTGGTCTCGCGCTTCGACT
CTGGGCTGACGGTGGCGATCGAGCCGCCCGAGCTGGAAATGCGCGTGGCCATCCTGATCAACAAGGCGCGCGCTGAAGGG
TCGGAGATGCCCGAAGAGGTGGCCTTCTTCGTGGCCAAGAATGTGCGCTCCAACGTGCGCGAACTCGAAGGCGCGCTGCG
CAAGATCCTGGCCTACAGCCGCTTCAACCAGAAGGAAGTTTCCATCCAACTCGCGCGCGAAGCGCTGCGCGACCTGCTGT
CGATACAGAACCGGCAGATCTCGGTGGAAAACATCCAGAAGACCGTGGCCGACTATTACAAGATCAAGGTCGCGGACATG
TACAGCAAGAAGCGTCCAGCCAGCATCGCCCGCCCGCGGCAGATCGCCATGTACCTGGCCAAGGAGCTCACGCAAAAGAG
CCTGCCCGAGATCGGCGAGCTGTTCGGCGGGCGCGACCACACCACGGTGCTCCACGCGGTGCGCAAGATTGGCGCCGAAC
GCCAGCAGCTCACCGAACTCAACCAGCAGCTGCACGTGCTCGAGCAGACGCTGAAGGGTTGA

Upstream 100 bases:

>100_bases
TGCCCCCTTACCGCCTCGCTCAGATTGTGGATAACTCCTTGACAAGCTAGAATCGCGGGCCTTGTACCGTTCCTCCTATC
CACAACAAGAATCACCTGAA

Downstream 100 bases:

>100_bases
CGCACCCCATGGAGCGAAAAGGACCGCCCCCCGCAAAAGCACGCCCGTTCGCGGGCAAAATGGCGCGTTGGACGGCCCGG
GAGGGGGCACTGCCCATGCC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNEGLHSSSHDSDGASAGQGLWQACAEQLAQDLPEQQFNTWIKPLVAQVAPDFSKVTLLVANRFKLDWIRAQYAGRISAL
LEGLYGQPVTLELALAQRESVVRTYVRPTAPETPATGDAPSAHPTADEAPAGAFRTRLNPALTFETLVEGTANRMARSAA
MHVAGSPGHLYNPLFIYGGVGLGKTHLVHAVGNKLLQDRPDAKVLYIHAEQFVSDVVKSYQRRTFDDFKERYHSLDLLLI
DDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPKGLANIHERLVSRFDSGLTVAIEPPELEMRVAILINKARAEG
SEMPEEVAFFVAKNVRSNVRELEGALRKILAYSRFNQKEVSIQLAREALRDLLSIQNRQISVENIQKTVADYYKIKVADM
YSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGAERQQLTELNQQLHVLEQTLKG

Sequences:

>Translated_473_residues
MNEGLHSSSHDSDGASAGQGLWQACAEQLAQDLPEQQFNTWIKPLVAQVAPDFSKVTLLVANRFKLDWIRAQYAGRISAL
LEGLYGQPVTLELALAQRESVVRTYVRPTAPETPATGDAPSAHPTADEAPAGAFRTRLNPALTFETLVEGTANRMARSAA
MHVAGSPGHLYNPLFIYGGVGLGKTHLVHAVGNKLLQDRPDAKVLYIHAEQFVSDVVKSYQRRTFDDFKERYHSLDLLLI
DDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPKGLANIHERLVSRFDSGLTVAIEPPELEMRVAILINKARAEG
SEMPEEVAFFVAKNVRSNVRELEGALRKILAYSRFNQKEVSIQLAREALRDLLSIQNRQISVENIQKTVADYYKIKVADM
YSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGAERQQLTELNQQLHVLEQTLKG
>Mature_473_residues
MNEGLHSSSHDSDGASAGQGLWQACAEQLAQDLPEQQFNTWIKPLVAQVAPDFSKVTLLVANRFKLDWIRAQYAGRISAL
LEGLYGQPVTLELALAQRESVVRTYVRPTAPETPATGDAPSAHPTADEAPAGAFRTRLNPALTFETLVEGTANRMARSAA
MHVAGSPGHLYNPLFIYGGVGLGKTHLVHAVGNKLLQDRPDAKVLYIHAEQFVSDVVKSYQRRTFDDFKERYHSLDLLLI
DDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPKGLANIHERLVSRFDSGLTVAIEPPELEMRVAILINKARAEG
SEMPEEVAFFVAKNVRSNVRELEGALRKILAYSRFNQKEVSIQLAREALRDLLSIQNRQISVENIQKTVADYYKIKVADM
YSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGAERQQLTELNQQLHVLEQTLKG

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=467, Percent_Identity=50.321199143469, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI226510964, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52854; Mature: 52854

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEGLHSSSHDSDGASAGQGLWQACAEQLAQDLPEQQFNTWIKPLVAQVAPDFSKVTLLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ANRFKLDWIRAQYAGRISALLEGLYGQPVTLELALAQRESVVRTYVRPTAPETPATGDAP
HCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
SAHPTADEAPAGAFRTRLNPALTFETLVEGTANRMARSAAMHVAGSPGHLYNPLFIYGGV
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
GLGKTHLVHAVGNKLLQDRPDAKVLYIHAEQFVSDVVKSYQRRTFDDFKERYHSLDLLLI
CCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEE
DDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPKGLANIHERLVSRFDSGLTVAI
CCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
EPPELEMRVAILINKARAEGSEMPEEVAFFVAKNVRSNVRELEGALRKILAYSRFNQKEV
CCCCCCEEEEHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SIQLAREALRDLLSIQNRQISVENIQKTVADYYKIKVADMYSKKRPASIARPRQIAMYLA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KELTQKSLPEIGELFGGRDHTTVLHAVRKIGAERQQLTELNQQLHVLEQTLKG
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEGLHSSSHDSDGASAGQGLWQACAEQLAQDLPEQQFNTWIKPLVAQVAPDFSKVTLLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ANRFKLDWIRAQYAGRISALLEGLYGQPVTLELALAQRESVVRTYVRPTAPETPATGDAP
HCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
SAHPTADEAPAGAFRTRLNPALTFETLVEGTANRMARSAAMHVAGSPGHLYNPLFIYGGV
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
GLGKTHLVHAVGNKLLQDRPDAKVLYIHAEQFVSDVVKSYQRRTFDDFKERYHSLDLLLI
CCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEE
DDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPKGLANIHERLVSRFDSGLTVAI
CCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
EPPELEMRVAILINKARAEGSEMPEEVAFFVAKNVRSNVRELEGALRKILAYSRFNQKEV
CCCCCCEEEEHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SIQLAREALRDLLSIQNRQISVENIQKTVADYYKIKVADMYSKKRPASIARPRQIAMYLA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KELTQKSLPEIGELFGGRDHTTVLHAVRKIGAERQQLTELNQQLHVLEQTLKG
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA