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The map label for this gene is dnaN [H]

Identifier: 222109227

GI number: 222109227

Start: 1636

End: 2742

Strand: Direct

Name: dnaN [H]

Synonym: Dtpsy_0002

Alternate gene names: 222109227

Gene position: 1636-2742 (Clockwise)

Preceding gene: 222109226

Following gene: 222109228

Centisome position: 0.04

GC content: 64.14

Gene sequence:

>1107_bases
ATGATTGTCCTGAAGGCCACACAAGACAAGGTTCTCGCCGTTCTGCAATCGGTTGCCGGCATCGTCGAGCGCCGCCACAC
GCTGCCCATCCTGGCCAATGTGCTGCTGCGCAAGACGGGCAATGCCATCCAGCTCACCACCAGCGACCTGGAGATCCAGA
TCCGCACCACGGCCGAGCTGGGCGGCGACACGGGCGACTTCACCACCACGGTGGGTGCACGCAAGCTCATCGACATCCTG
AAGACCATGCCCGCCGACCAGACGGTGAGCCTGGAGTCCAACCAGTCCAAGCTGATCCTGAAGGGCGGCAAGAGCCGCTT
CACACTGCAGACGCTGCCGGCCGAGGACTTCCCGCTGGTGCAGGAGGCCGCCAGCTTCGGCCCCGCCTTCAGCGTGCCGC
AGAAGGTCCTGAAGAGCCTGCTGGGGCAGGTGTCGTTCGCCATGGCAGTGCAGGACATCCGCTACTACCTGAACGGCATC
CTGTTCGTGGCCGAGGGCACTACGCTCTCGCTGGTCGCCACCGACGGCCACCGGCTGGCGTTCGCCAGCGCGCAGCTGGA
CGTGGAAGTGCCCAAGCAGGAGGTCATCTTGCCGCGCAAGACCGTGCTGGAGTTGCAGCGTCTGCTGTCGGACGCCGACG
GCGCCATCGAGATGCAGTTCGCCAACAACCAGGCCAAGTTCAGCTTCGGCGGCATGGAGTTCGTCACCAAGCTGGTGGAG
GGCAAGTTCCCCGACTACAACCGCGTGATTCCCAAGAACCACCACAACAGCGTGACCCTGGGCCGCGCCCCGCTGCTGGC
CAGCCTGCAGCGCACGGCCATCATGACCAGCGACAAGTTCAAGGGCGTGCGCCTGAACATCGAACCCGGCCTGCTGCGTG
TGGCGTCGAACAACGCCGAGCAGGAAGAGGCGGTGGACGAGCTGGACATCGACTACGGCGGTGACGCCATCGAGATCGGC
TTCAACGTCAGCTACCTCATCGACGCCCTGGCCAACATGGGCCAGGACATGGTGAAGGTCGACCTGGCCGATGGCAACAG
CTCCGCGTTGATGACCATCCCCGACAACGAGCACTTCAAGTACGTCGTGATGCCCATGCGCATCTGA

Upstream 100 bases:

>100_bases
CCGTTCGCGGGCAAAATGGCGCGTTGGACGGCCCGGGAGGGGGCACTGCCCATGCCGCCGTCTGCGCACCTATCCCCAAA
AACCACCAAAGGTTGACGAC

Downstream 100 bases:

>100_bases
CATACTATTATTTTGATAGCTGTTAGCGCTTGATGGACGGGCGCTAGCGGCACATTTGATTCCTATAAGGCCGCCGGCAC
GCCGTGCCGGTGCGCCTGGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLLRKTGNAIQLTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDIL
KTMPADQTVSLESNQSKLILKGGKSRFTLQTLPAEDFPLVQEAASFGPAFSVPQKVLKSLLGQVSFAMAVQDIRYYLNGI
LFVAEGTTLSLVATDGHRLAFASAQLDVEVPKQEVILPRKTVLELQRLLSDADGAIEMQFANNQAKFSFGGMEFVTKLVE
GKFPDYNRVIPKNHHNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGLLRVASNNAEQEEAVDELDIDYGGDAIEIG
FNVSYLIDALANMGQDMVKVDLADGNSSALMTIPDNEHFKYVVMPMRI

Sequences:

>Translated_368_residues
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLLRKTGNAIQLTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDIL
KTMPADQTVSLESNQSKLILKGGKSRFTLQTLPAEDFPLVQEAASFGPAFSVPQKVLKSLLGQVSFAMAVQDIRYYLNGI
LFVAEGTTLSLVATDGHRLAFASAQLDVEVPKQEVILPRKTVLELQRLLSDADGAIEMQFANNQAKFSFGGMEFVTKLVE
GKFPDYNRVIPKNHHNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGLLRVASNNAEQEEAVDELDIDYGGDAIEIG
FNVSYLIDALANMGQDMVKVDLADGNSSALMTIPDNEHFKYVVMPMRI
>Mature_368_residues
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLLRKTGNAIQLTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDIL
KTMPADQTVSLESNQSKLILKGGKSRFTLQTLPAEDFPLVQEAASFGPAFSVPQKVLKSLLGQVSFAMAVQDIRYYLNGI
LFVAEGTTLSLVATDGHRLAFASAQLDVEVPKQEVILPRKTVLELQRLLSDADGAIEMQFANNQAKFSFGGMEFVTKLVE
GKFPDYNRVIPKNHHNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGLLRVASNNAEQEEAVDELDIDYGGDAIEIG
FNVSYLIDALANMGQDMVKVDLADGNSSALMTIPDNEHFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=361, Percent_Identity=40.7202216066482, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40146; Mature: 40146

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLLRKTGNAIQLTTSDLEIQIRTTAEL
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEEHCC
GGDTGDFTTTVGARKLIDILKTMPADQTVSLESNQSKLILKGGKSRFTLQTLPAEDFPLV
CCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCHHH
QEAASFGPAFSVPQKVLKSLLGQVSFAMAVQDIRYYLNGILFVAEGTTLSLVATDGHRLA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEEECCCEEE
FASAQLDVEVPKQEVILPRKTVLELQRLLSDADGAIEMQFANNQAKFSFGGMEFVTKLVE
EEECEEEEECCHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECHHHHHHHHHC
GKFPDYNRVIPKNHHNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGLLRVASNNAE
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHEEECCCCCCEEEEECCCEEEEECCCCH
QEEAVDELDIDYGGDAIEIGFNVSYLIDALANMGQDMVKVDLADGNSSALMTIPDNEHFK
HHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC
>Mature Secondary Structure
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLLRKTGNAIQLTTSDLEIQIRTTAEL
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEEHCC
GGDTGDFTTTVGARKLIDILKTMPADQTVSLESNQSKLILKGGKSRFTLQTLPAEDFPLV
CCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCHHH
QEAASFGPAFSVPQKVLKSLLGQVSFAMAVQDIRYYLNGILFVAEGTTLSLVATDGHRLA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEEECCCEEE
FASAQLDVEVPKQEVILPRKTVLELQRLLSDADGAIEMQFANNQAKFSFGGMEFVTKLVE
EEECEEEEECCHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECHHHHHHHHHC
GKFPDYNRVIPKNHHNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGLLRVASNNAE
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHEEECCCCCCEEEEECCCEEEEECCCCH
QEEAVDELDIDYGGDAIEIGFNVSYLIDALANMGQDMVKVDLADGNSSALMTIPDNEHFK
HHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]