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The map label for this gene is paaF [C]

Identifier: 222102934

GI number: 222102934

Start: 621801

End: 622601

Strand: Direct

Name: paaF [C]

Synonym: Avi_7690

Alternate gene names: 222102934

Gene position: 621801-622601 (Clockwise)

Preceding gene: 222102933

Following gene: 222102935

Centisome position: 98.42

GC content: 59.8

Gene sequence:

>801_bases
ATGATCTATAAAACCCTTGATCTCGAATTGAACGGTGCGGTCGCAACAGTGTGGATGAACCGGCCAGACCTGCACAATGC
CTTCGATGAGATGCTCATTGAGGACATGACGCTGGCCATTGAAGCGCTGTCTGAAAACGACAGCGTGCGCGTCCTTGTGC
TGGCCGGTCGTGGGGCCAGTTTTTCCGCAGGCGCTGACCTTGCGTGGATGAAGCGGCAAGGTGCTGCTACCCTTGCGGAC
AATACGGCGGATGCCGCCGCCATGGGGCGGATGTTCATGGCATTGCGCAATTGTCCCAAGCCGCTGATTGCCCGCATTCA
AGGTGCTGCCATTGGCGGTGGAATGGGGCTGGTGGCGGCCTGCGACATTGCTGTTGCCGCTCCCGATGCCGTGTTTGCCA
CTTCAGAAGTTCGCCTCGCGCTGATCCCTGCGGTTATCAGCCCGCTGGTTGCTGCTGCGATTGGGGAGCGTCAGTGCCGC
CGCTATTTCCTCACGGGGGAGCGGATGGGGGTTGAGAAGGCACATGTGCTGGGCCTTGTGCACGAGATTGCCGAAAAGGA
TGGCCTTGACGCTGCTGTGGCGCGGATTGTTGCCGATGTGCTGAAAGGCGCGCCTGGCGCGGTAACAGAGGCGAAGGCGT
TGATCACGCAGATTTCCGACCGTCGTTTCGATGAAGCATTGGTGTCTGAAATGGCAGAGCGGATTGCGGCCCGCCGAGGC
ACGGATGAAGCCCGCGAGGGTCTTTCGGCCTTTCTGGAAAAACGCAAACCCGGCTGGATCGCGGATCGGAAAGAAGGGTG
A

Upstream 100 bases:

>100_bases
CCCGCGCAAACCCGCCGGGTTCTCGGCCTTTCCCTGTCAACGGCGCTCAATGCGCCCATCGGTGAAACCCGCTTCGGCCT
TTTCCGCATGTGAGATTGCC

Downstream 100 bases:

>100_bases
AGGATCAAACCATGTTTCAGAAAATCCTGATCGCCAATCGCGGCGAAATCGCCTGCCGCATTATCCGAACCGCGCGGCGC
ATGGGCATTGCCACCGTTGC

Product: Enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MIYKTLDLELNGAVATVWMNRPDLHNAFDEMLIEDMTLAIEALSENDSVRVLVLAGRGASFSAGADLAWMKRQGAATLAD
NTADAAAMGRMFMALRNCPKPLIARIQGAAIGGGMGLVAACDIAVAAPDAVFATSEVRLALIPAVISPLVAAAIGERQCR
RYFLTGERMGVEKAHVLGLVHEIAEKDGLDAAVARIVADVLKGAPGAVTEAKALITQISDRRFDEALVSEMAERIAARRG
TDEAREGLSAFLEKRKPGWIADRKEG

Sequences:

>Translated_266_residues
MIYKTLDLELNGAVATVWMNRPDLHNAFDEMLIEDMTLAIEALSENDSVRVLVLAGRGASFSAGADLAWMKRQGAATLAD
NTADAAAMGRMFMALRNCPKPLIARIQGAAIGGGMGLVAACDIAVAAPDAVFATSEVRLALIPAVISPLVAAAIGERQCR
RYFLTGERMGVEKAHVLGLVHEIAEKDGLDAAVARIVADVLKGAPGAVTEAKALITQISDRRFDEALVSEMAERIAARRG
TDEAREGLSAFLEKRKPGWIADRKEG
>Mature_266_residues
MIYKTLDLELNGAVATVWMNRPDLHNAFDEMLIEDMTLAIEALSENDSVRVLVLAGRGASFSAGADLAWMKRQGAATLAD
NTADAAAMGRMFMALRNCPKPLIARIQGAAIGGGMGLVAACDIAVAAPDAVFATSEVRLALIPAVISPLVAAAIGERQCR
RYFLTGERMGVEKAHVLGLVHEIAEKDGLDAAVARIVADVLKGAPGAVTEAKALITQISDRRFDEALVSEMAERIAARRG
TDEAREGLSAFLEKRKPGWIADRKEG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=28.2868525896414, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=27.4509803921569, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI70995211, Length=136, Percent_Identity=34.5588235294118, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=24.3137254901961, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=24.3137254901961, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=24.3137254901961, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=29.0322580645161, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=25.8064516129032, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=28.9473684210526, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=23.8805970149254, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=29.277566539924, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=27.9527559055118, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=30.3643724696356, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=26.7543859649123, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=28.3783783783784, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=24.8062015503876, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=143, Percent_Identity=30.0699300699301, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=143, Percent_Identity=30.0699300699301, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=143, Percent_Identity=30.0699300699301, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=179, Percent_Identity=28.4916201117318, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=136, Percent_Identity=27.9411764705882, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=24.1666666666667, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653139, Length=273, Percent_Identity=28.9377289377289, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=29.7169811320755, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=203, Percent_Identity=24.6305418719212, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=21.6535433070866, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28283; Mature: 28283

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYKTLDLELNGAVATVWMNRPDLHNAFDEMLIEDMTLAIEALSENDSVRVLVLAGRGAS
CCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FSAGADLAWMKRQGAATLADNTADAAAMGRMFMALRNCPKPLIARIQGAAIGGGMGLVAA
CCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHH
CDIAVAAPDAVFATSEVRLALIPAVISPLVAAAIGERQCRRYFLTGERMGVEKAHVLGLV
HHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
HEIAEKDGLDAAVARIVADVLKGAPGAVTEAKALITQISDRRFDEALVSEMAERIAARRG
HHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDEAREGLSAFLEKRKPGWIADRKEG
CHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIYKTLDLELNGAVATVWMNRPDLHNAFDEMLIEDMTLAIEALSENDSVRVLVLAGRGAS
CCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FSAGADLAWMKRQGAATLADNTADAAAMGRMFMALRNCPKPLIARIQGAAIGGGMGLVAA
CCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHH
CDIAVAAPDAVFATSEVRLALIPAVISPLVAAAIGERQCRRYFLTGERMGVEKAHVLGLV
HHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
HEIAEKDGLDAAVARIVADVLKGAPGAVTEAKALITQISDRRFDEALVSEMAERIAARRG
HHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDEAREGLSAFLEKRKPGWIADRKEG
CHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA