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The map label for this gene is thcD [H]

Identifier: 222102927

GI number: 222102927

Start: 612135

End: 613373

Strand: Reverse

Name: thcD [H]

Synonym: Avi_7682

Alternate gene names: 222102927

Gene position: 613373-612135 (Counterclockwise)

Preceding gene: 222102928

Following gene: 222102926

Centisome position: 97.09

GC content: 60.05

Gene sequence:

>1239_bases
ATGAACCAGGTGGCCATCATTGGCTCCGGCCAGGGAGGGTTCCAGTTGGCTGCGAGCCTTCGTCAGGAGGGGTTTTCCGG
GGAGATCACCTTGATCGGAGAGGAAACAGGCCTGCCTTACCAGCGTCCGCCGCTTTCCAAGGCTTATTTAAAAGATGGCC
GTGCCGAGAGCATTGAGCTGAGGCCCGACAGCTTCTTTCACCGAAACAAAATCGGCCTGATAGCCGGGGACCCGGCCATT
CATATCGATCGGGTGACGCGAACTGTGGCGACCAAGAGCGGTGCCCGTATCGGCTATAACCATCTCGTGCTGGCAACCGG
CGCGCGTAACCTCATCCCGCCGATCCGTGGCCTGGAGGGGAGCGATGTCTTCGCCTTGCGCACCGCAGCCGATGCGGATG
CCCTGCGCGAAGCTCTCGCGCGGGGTCGTCGCCACCCTGTGGTGATCGGGGGCGGCTTTATCGGCCTCGAATTCGCCGCT
GTTGCGGCATCCATCGGCCACTCCGTCACCGTCGTCGAGGCAACGGAACGCCTTATGGCTCGTGCCGTTTCTCGCGCTAT
GTCCGGATTCTTTCAGACCTTCCACGAGATGCACGGCGTGTCGCTGCACTTCGGGGACCCCGTCAACGAGATCCTGCGGA
GTGAGGATGGCGGGGTTGTGGGTGTTCGCCTTCTTAGCGGAGCAATTATTCCCGGTGACATGGTGCTGCTTGCTGTTGGC
GTGCGACCCAATGTGGAACTTGCCAGAAACGCTGGGCTTGAGATTGCCAACGGTATCGCTGTGGATGCGTATCTTCTCAC
CGCCGATCCAGCCATTTCGGGCCTCGGCGACTGTGCAGCCTTTCCAGATCCGGTCACGGGGGAGTTGACGCGGCTTGAAT
CCGTCCAGGCCGCCACCGACCACGCCCGAACAATCGCTCGTCGGCTCACGGGAAAATCCGAACCCTATGAGGCCTTGCCT
TGGTTCTGGAGCGATCAGGGGCCATGGAAGTTGCAGATTGCAGGTCTTGCGGTATCGGGCGATGAGGATCATCCCCTTGA
AGGTGAAAACGGGCGAAGGCTTGTATTTCGCTTCAAATCGGGCAAATTGCGTGCAGTGGAGACGGTCAATGCCGCAGCCG
AGCACATGGCCGCACGTCAGCTTCTAAAGTCGCCAGAAGGGGTGACAGCCGATGAGCTTGTGCAATGCAACTATGATATC
ACGGCACTGTCGAAATCGAGGAAAGCACGCGCCGCATGA

Upstream 100 bases:

>100_bases
GCGTCATCTGCGCGATAAGGCCATCCAGCCGTCTCTCGTGCCAGATTCGCATCAAACCACATCTCGATGGTCTCGTCATC
CATCTTCCGGAGAGCCAAAC

Downstream 100 bases:

>100_bases
CATCTGCCAAGAGGCCAACAAGCGTGCGGGTGAAAACTGGCGCACGCTGCCCGCAAGACCGCATATCCAGTCCGACGACC
ACACGCGATGGCGTCGAAAT

Product: ferredoxin reductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNQVAIIGSGQGGFQLAASLRQEGFSGEITLIGEETGLPYQRPPLSKAYLKDGRAESIELRPDSFFHRNKIGLIAGDPAI
HIDRVTRTVATKSGARIGYNHLVLATGARNLIPPIRGLEGSDVFALRTAADADALREALARGRRHPVVIGGGFIGLEFAA
VAASIGHSVTVVEATERLMARAVSRAMSGFFQTFHEMHGVSLHFGDPVNEILRSEDGGVVGVRLLSGAIIPGDMVLLAVG
VRPNVELARNAGLEIANGIAVDAYLLTADPAISGLGDCAAFPDPVTGELTRLESVQAATDHARTIARRLTGKSEPYEALP
WFWSDQGPWKLQIAGLAVSGDEDHPLEGENGRRLVFRFKSGKLRAVETVNAAAEHMAARQLLKSPEGVTADELVQCNYDI
TALSKSRKARAA

Sequences:

>Translated_412_residues
MNQVAIIGSGQGGFQLAASLRQEGFSGEITLIGEETGLPYQRPPLSKAYLKDGRAESIELRPDSFFHRNKIGLIAGDPAI
HIDRVTRTVATKSGARIGYNHLVLATGARNLIPPIRGLEGSDVFALRTAADADALREALARGRRHPVVIGGGFIGLEFAA
VAASIGHSVTVVEATERLMARAVSRAMSGFFQTFHEMHGVSLHFGDPVNEILRSEDGGVVGVRLLSGAIIPGDMVLLAVG
VRPNVELARNAGLEIANGIAVDAYLLTADPAISGLGDCAAFPDPVTGELTRLESVQAATDHARTIARRLTGKSEPYEALP
WFWSDQGPWKLQIAGLAVSGDEDHPLEGENGRRLVFRFKSGKLRAVETVNAAAEHMAARQLLKSPEGVTADELVQCNYDI
TALSKSRKARAA
>Mature_412_residues
MNQVAIIGSGQGGFQLAASLRQEGFSGEITLIGEETGLPYQRPPLSKAYLKDGRAESIELRPDSFFHRNKIGLIAGDPAI
HIDRVTRTVATKSGARIGYNHLVLATGARNLIPPIRGLEGSDVFALRTAADADALREALARGRRHPVVIGGGFIGLEFAA
VAASIGHSVTVVEATERLMARAVSRAMSGFFQTFHEMHGVSLHFGDPVNEILRSEDGGVVGVRLLSGAIIPGDMVLLAVG
VRPNVELARNAGLEIANGIAVDAYLLTADPAISGLGDCAAFPDPVTGELTRLESVQAATDHARTIARRLTGKSEPYEALP
WFWSDQGPWKLQIAGLAVSGDEDHPLEGENGRRLVFRFKSGKLRAVETVNAAAEHMAARQLLKSPEGVTADELVQCNYDI
TALSKSRKARAA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=405, Percent_Identity=30.3703703703704, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI226437568, Length=405, Percent_Identity=30.3703703703704, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI21389617, Length=378, Percent_Identity=30.952380952381, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI22202629, Length=347, Percent_Identity=28.8184438040346, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4757732, Length=347, Percent_Identity=28.8184438040346, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=34.0796019900498, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789765, Length=304, Percent_Identity=30.2631578947368, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=29.6529968454259, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=325, Percent_Identity=27.3846153846154, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639257, Length=324, Percent_Identity=29.320987654321, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281359715, Length=324, Percent_Identity=29.320987654321, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281359713, Length=324, Percent_Identity=29.320987654321, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639250, Length=324, Percent_Identity=29.320987654321, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18543267, Length=324, Percent_Identity=29.320987654321, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639252, Length=324, Percent_Identity=29.320987654321, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585130, Length=328, Percent_Identity=26.5243902439024, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28573993, Length=362, Percent_Identity=29.2817679558011, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24581020, Length=362, Percent_Identity=29.2817679558011, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 43888; Mature: 43888

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVAIIGSGQGGFQLAASLRQEGFSGEITLIGEETGLPYQRPPLSKAYLKDGRAESIEL
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCEEEE
RPDSFFHRNKIGLIAGDPAIHIDRVTRTVATKSGARIGYNHLVLATGARNLIPPIRGLEG
CCCCHHHCCCEEEEECCCCEEHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCCCCC
SDVFALRTAADADALREALARGRRHPVVIGGGFIGLEFAAVAASIGHSVTVVEATERLMA
CCEEEEEECCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCEEEEEHHHHHHHH
RAVSRAMSGFFQTFHEMHGVSLHFGDPVNEILRSEDGGVVGVRLLSGAIIPGDMVLLAVG
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEC
VRPNVELARNAGLEIANGIAVDAYLLTADPAISGLGDCAAFPDPVTGELTRLESVQAATD
CCCCHHHHHCCCCEEECCEEEEEEEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HARTIARRLTGKSEPYEALPWFWSDQGPWKLQIAGLAVSGDEDHPLEGENGRRLVFRFKS
HHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEECC
GKLRAVETVNAAAEHMAARQLLKSPEGVTADELVQCNYDITALSKSRKARAA
CCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MNQVAIIGSGQGGFQLAASLRQEGFSGEITLIGEETGLPYQRPPLSKAYLKDGRAESIEL
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCEEEE
RPDSFFHRNKIGLIAGDPAIHIDRVTRTVATKSGARIGYNHLVLATGARNLIPPIRGLEG
CCCCHHHCCCEEEEECCCCEEHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCCCCC
SDVFALRTAADADALREALARGRRHPVVIGGGFIGLEFAAVAASIGHSVTVVEATERLMA
CCEEEEEECCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCEEEEEHHHHHHHH
RAVSRAMSGFFQTFHEMHGVSLHFGDPVNEILRSEDGGVVGVRLLSGAIIPGDMVLLAVG
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEC
VRPNVELARNAGLEIANGIAVDAYLLTADPAISGLGDCAAFPDPVTGELTRLESVQAATD
CCCCHHHHHCCCCEEECCEEEEEEEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HARTIARRLTGKSEPYEALPWFWSDQGPWKLQIAGLAVSGDEDHPLEGENGRRLVFRFKS
HHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEECC
GKLRAVETVNAAAEHMAARQLLKSPEGVTADELVQCNYDITALSKSRKARAA
CCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]