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The map label for this gene is nac [H]

Identifier: 222102903

GI number: 222102903

Start: 589053

End: 589952

Strand: Direct

Name: nac [H]

Synonym: Avi_7653

Alternate gene names: 222102903

Gene position: 589053-589952 (Clockwise)

Preceding gene: 222102901

Following gene: 222102904

Centisome position: 93.24

GC content: 57.67

Gene sequence:

>900_bases
ATGCGCTATTTTGCGCGCATTGCAGAGCTAGGCTCCTTTACCCGTGCCGCCAGTGAGCTGGGTGTAGCCCAACCAGCGCT
GAGCTTGCATGTGCGCGCGCTGGAAGACCAACTGGGGCTGCAATTGCTCAACCGGACCTCGCGTGGCGTCGTGGTAACGG
AGGCAGGCCAAACGCTGCTGGTCCATGCTCAGGCAATCCTTCGGGCGGTAGAGCAGGCCGAAGCCGCAACGCGGGAGCAG
GCGAAATATCCAAGTGGTGATGTCTCGCTTGGTATTCTCAGCTCGCTGTCTCCCATTCTCTCCATTCCGGTTCTGGAAGC
ATGTGACCGCCGGTTTCCAAAAATCCGCCTGACGATCAGCGAGGGCGATAGCCAGACCCTGCGCACAGCGACCGATATCC
ATGCGCATGATCTAGCCGTGAACCTTCTGGATGTTGCCAAACCAACGGCGGTGCCTTTGTTTGACGAGCGGCTCTATGCC
GTCGGGCCTCTGAATGCCTTTACCCAAAGGCAGAATGATTTGCCGCTCCGCGAAGCTTTGAGCCTTCCGCTGATCATGCC
CTCGCAGCGTCATGGAATTCGCATCCTTTTGGAAAAGCAGGCCCTTATTCTTGGTCAAAAAATCAATATTGTTCGGGTGA
TCGAGGGAGTGGCCAGCACCAAAGCGGCTATCCGTGCCGGGTTGGGATTGACCATTCTGGGTCGGGGAGCCGTCTACGCC
GATCATCAGGCAGGACAACTCTCGGTCATTCCACTGAGGGCACCGGAGCTGTTTCGACGCCTTGTGCTGGATATGCCGGT
CAATCATCCACCGACCAAGGCGGTTCTTGAGGTGAGACAGATCCTGATGGAGGTCATCAAGCGTCTCGGTGGAGAGGGCC
ATTGGAGCTGTCTCGCCTAA

Upstream 100 bases:

>100_bases
TTTTAAGAGGCTTGCGCTATGCCAACAAATATATATTGACGATACTTGATATATGAATGCTGTATATCAGGAGGCAGCCT
TGGCATTGGATGTAACAAAA

Downstream 100 bases:

>100_bases
GAACTGCGCCCCGCTTTTCCATGGCGGGCGCGCACCATTCAAACTTTGTTTGCCCCCATCACAGGAAGGGTCGTGCTAGC
TGTTTTTGAACGTCTTTACG

Product: Transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRYFARIAELGSFTRAASELGVAQPALSLHVRALEDQLGLQLLNRTSRGVVVTEAGQTLLVHAQAILRAVEQAEAATREQ
AKYPSGDVSLGILSSLSPILSIPVLEACDRRFPKIRLTISEGDSQTLRTATDIHAHDLAVNLLDVAKPTAVPLFDERLYA
VGPLNAFTQRQNDLPLREALSLPLIMPSQRHGIRILLEKQALILGQKINIVRVIEGVASTKAAIRAGLGLTILGRGAVYA
DHQAGQLSVIPLRAPELFRRLVLDMPVNHPPTKAVLEVRQILMEVIKRLGGEGHWSCLA

Sequences:

>Translated_299_residues
MRYFARIAELGSFTRAASELGVAQPALSLHVRALEDQLGLQLLNRTSRGVVVTEAGQTLLVHAQAILRAVEQAEAATREQ
AKYPSGDVSLGILSSLSPILSIPVLEACDRRFPKIRLTISEGDSQTLRTATDIHAHDLAVNLLDVAKPTAVPLFDERLYA
VGPLNAFTQRQNDLPLREALSLPLIMPSQRHGIRILLEKQALILGQKINIVRVIEGVASTKAAIRAGLGLTILGRGAVYA
DHQAGQLSVIPLRAPELFRRLVLDMPVNHPPTKAVLEVRQILMEVIKRLGGEGHWSCLA
>Mature_299_residues
MRYFARIAELGSFTRAASELGVAQPALSLHVRALEDQLGLQLLNRTSRGVVVTEAGQTLLVHAQAILRAVEQAEAATREQ
AKYPSGDVSLGILSSLSPILSIPVLEACDRRFPKIRLTISEGDSQTLRTATDIHAHDLAVNLLDVAKPTAVPLFDERLYA
VGPLNAFTQRQNDLPLREALSLPLIMPSQRHGIRILLEKQALILGQKINIVRVIEGVASTKAAIRAGLGLTILGRGAVYA
DHQAGQLSVIPLRAPELFRRLVLDMPVNHPPTKAVLEVRQILMEVIKRLGGEGHWSCLA

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=240, Percent_Identity=33.75, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=28.4615384615385, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI145693105, Length=235, Percent_Identity=23.4042553191489, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30.625, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787879, Length=305, Percent_Identity=26.5573770491803, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787589, Length=121, Percent_Identity=38.8429752066116, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789173, Length=133, Percent_Identity=33.8345864661654, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=121, Percent_Identity=33.8842975206612, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=122, Percent_Identity=32.7868852459016, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=121, Percent_Identity=32.2314049586777, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=236, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693193, Length=123, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787806, Length=124, Percent_Identity=33.0645161290323, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082132, Length=263, Percent_Identity=22.0532319391635, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788887, Length=176, Percent_Identity=30.6818181818182, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32521; Mature: 32521

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYFARIAELGSFTRAASELGVAQPALSLHVRALEDQLGLQLLNRTSRGVVVTEAGQTLL
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEH
VHAQAILRAVEQAEAATREQAKYPSGDVSLGILSSLSPILSIPVLEACDRRFPKIRLTIS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEE
EGDSQTLRTATDIHAHDLAVNLLDVAKPTAVPLFDERLYAVGPLNAFTQRQNDLPLREAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCHHHHCCCCCCCHHHHC
SLPLIMPSQRHGIRILLEKQALILGQKINIVRVIEGVASTKAAIRAGLGLTILGRGAVYA
CCCEECCCCCCCEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEE
DHQAGQLSVIPLRAPELFRRLVLDMPVNHPPTKAVLEVRQILMEVIKRLGGEGHWSCLA
CCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MRYFARIAELGSFTRAASELGVAQPALSLHVRALEDQLGLQLLNRTSRGVVVTEAGQTLL
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEH
VHAQAILRAVEQAEAATREQAKYPSGDVSLGILSSLSPILSIPVLEACDRRFPKIRLTIS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEE
EGDSQTLRTATDIHAHDLAVNLLDVAKPTAVPLFDERLYAVGPLNAFTQRQNDLPLREAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCHHHHCCCCCCCHHHHC
SLPLIMPSQRHGIRILLEKQALILGQKINIVRVIEGVASTKAAIRAGLGLTILGRGAVYA
CCCEECCCCCCCEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEE
DHQAGQLSVIPLRAPELFRRLVLDMPVNHPPTKAVLEVRQILMEVIKRLGGEGHWSCLA
CCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]