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The map label for this gene is gsiB [H]

Identifier: 222102800

GI number: 222102800

Start: 463236

End: 464933

Strand: Direct

Name: gsiB [H]

Synonym: Avi_7516

Alternate gene names: 222102800

Gene position: 463236-464933 (Clockwise)

Preceding gene: 222102799

Following gene: 222102801

Centisome position: 73.32

GC content: 52.41

Gene sequence:

>1698_bases
ATGCCTGAATGCCTGCAAACAGGTGAGAAGCCATCCATTGAAGGTGGCAAGAAGGAGCATTTTTACCGTTCGATAAAAAG
CCAAAATGCCGCAATCGCAATCAAAAAAATGGGGAACGAGATCGTCATGAAAACGGCTATCAAACGCAGAAACTTTATCA
AAGCAGGCGCATCGCTGGCCGCATTCGGCCTGTTGGGCGATTTTGCAAACCAAGCAATGGCGAAAAGTGTCGAGGGGGGC
ACATTAAAAATCGGCACCAGCAATGAGCTGAATTACAACATGCTGTGCTTCGCCATGACCGGTGGCCCCTACGACTATGT
CTATTCCTGGCCCATTTATGAATCGCTTTTCAAACCCAATGCCAATGGAACGATTGATCCCTGGCTTGCGGAATCGGTGG
AAAGTGATGCCAAAGCGCTCACCTATACCATCCATATCCGGCGTGGCGTGACATTTTCCGATGGATCGGTGCTGAACGCC
ACTGTGGTGAAATGGAATCTCGATCACTACCTGAAAGTCGGAGCAAAGCGCGTCGCCTTGCTCGGCTCCATCACGTCGGT
CGATGTGTTAGACGACTATACTGTGCGCCTGGTGCTCTCAAAATGGAGTTCGTTCATTCCCGCCGCGCTGTCGCGTGAGT
GCGGCTATATGTTCTCACAACAGCACTATGAAAAATACGGCGATGACTATTGCCAGCAGCACCCGGTTGGAACCGGCCCC
TTCGTATTGAAGGAATGGGTGCGGGATGTCAGCAAATCATTCGTGCGCAATGAACACTATTGGGGCGGCAAGGTTCTTCT
CGAGGCCGTTCAGTATACGATCTATAATGACTCGCTGGTGGGGCAGGCGGCCCTGATGTCGGATGAAATCGACGTGATGA
TTGGACTGGGCCTGAATGGTGTCAAAACGCTCGCCGCCAACGGCTTTGATGCCCCGATTGAGCCATTGCAAGACCATGCG
TCGCTGTTGGTGTTCAACTCCCTCAACACCAGCAATGACGATCCGATGGGCAATTTGCTGGTGCGCCAAGCCATCAGCCA
TGCCATCAATAGAAAATCGATCATAGAGGCAGCATTTCTGGGCTATGCCGATATTGCCAGCCAGTTTGGTATTGGCAGCC
ATTTCGTCAACAAGGACATTGTCGGTTACGATTACAATGTTGCCAAGGCGAAGGAACTGCTTGCGAAAGCGGGTTTCCCT
CAAGGCTTCTCGACAAAAATTCAGGCCGCCGCCAGTGATACCAACAAGATCGTCCTGCAAATCATGCAGGCCGATCTGGC
CAAAGTCGGCATACAAGCCAAAATCGAATTGCTGACGGGGGCTGCTTCCAACAAGGCTGAAACCGGTTGGGGAAGCGGCA
TGTGGTACCACACATCCAGCGTCTATGTGGATGTGGCCATGCAAATGTCCAGCATGTTTCCAAAAGGACTCACCGGCGGT
GTTCTTGGCCTGACCACCATGTTGCGCCCTGATGACGTGAGTGATGCGCTTGTACGGGCCGTATCATCCACCTCGGATGC
GGAATCGGTCAAAGCGGTTGGTGAAGCCAACAAGATGATGATCGACACCTACGCGCTCTACGTGCCCATTGCCCAATATT
CGACGATTTACGTTCTCAGCAAACGCGTCAAGGCATCAGGTATCGGCGCGACCTTCTTCTCCGTTGCCTCTCTCTCCACC
GCGTATCTCGACACGTGA

Upstream 100 bases:

>100_bases
TTCTGACGACATGACAGTGCCTTCGGCTGATTAAAGACCTCTTTCAGCACCGTTACAGGGGTGTTTCCTTCAATGGAGCG
GGATTGAAGGCCGCTTGCGC

Downstream 100 bases:

>100_bases
TCACTTATCTGTTGAAACGGCTGGCTATTGCTGGCTTCCTGATGCTGCTGGTATCCGCGCTGGTGTTTTTACTGATGCGC
GGATTACCGGGCGATCCTGC

Product: ABC transporter substrate binding protein (oligopeptide)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MPECLQTGEKPSIEGGKKEHFYRSIKSQNAAIAIKKMGNEIVMKTAIKRRNFIKAGASLAAFGLLGDFANQAMAKSVEGG
TLKIGTSNELNYNMLCFAMTGGPYDYVYSWPIYESLFKPNANGTIDPWLAESVESDAKALTYTIHIRRGVTFSDGSVLNA
TVVKWNLDHYLKVGAKRVALLGSITSVDVLDDYTVRLVLSKWSSFIPAALSRECGYMFSQQHYEKYGDDYCQQHPVGTGP
FVLKEWVRDVSKSFVRNEHYWGGKVLLEAVQYTIYNDSLVGQAALMSDEIDVMIGLGLNGVKTLAANGFDAPIEPLQDHA
SLLVFNSLNTSNDDPMGNLLVRQAISHAINRKSIIEAAFLGYADIASQFGIGSHFVNKDIVGYDYNVAKAKELLAKAGFP
QGFSTKIQAAASDTNKIVLQIMQADLAKVGIQAKIELLTGAASNKAETGWGSGMWYHTSSVYVDVAMQMSSMFPKGLTGG
VLGLTTMLRPDDVSDALVRAVSSTSDAESVKAVGEANKMMIDTYALYVPIAQYSTIYVLSKRVKASGIGATFFSVASLST
AYLDT

Sequences:

>Translated_565_residues
MPECLQTGEKPSIEGGKKEHFYRSIKSQNAAIAIKKMGNEIVMKTAIKRRNFIKAGASLAAFGLLGDFANQAMAKSVEGG
TLKIGTSNELNYNMLCFAMTGGPYDYVYSWPIYESLFKPNANGTIDPWLAESVESDAKALTYTIHIRRGVTFSDGSVLNA
TVVKWNLDHYLKVGAKRVALLGSITSVDVLDDYTVRLVLSKWSSFIPAALSRECGYMFSQQHYEKYGDDYCQQHPVGTGP
FVLKEWVRDVSKSFVRNEHYWGGKVLLEAVQYTIYNDSLVGQAALMSDEIDVMIGLGLNGVKTLAANGFDAPIEPLQDHA
SLLVFNSLNTSNDDPMGNLLVRQAISHAINRKSIIEAAFLGYADIASQFGIGSHFVNKDIVGYDYNVAKAKELLAKAGFP
QGFSTKIQAAASDTNKIVLQIMQADLAKVGIQAKIELLTGAASNKAETGWGSGMWYHTSSVYVDVAMQMSSMFPKGLTGG
VLGLTTMLRPDDVSDALVRAVSSTSDAESVKAVGEANKMMIDTYALYVPIAQYSTIYVLSKRVKASGIGATFFSVASLST
AYLDT
>Mature_564_residues
PECLQTGEKPSIEGGKKEHFYRSIKSQNAAIAIKKMGNEIVMKTAIKRRNFIKAGASLAAFGLLGDFANQAMAKSVEGGT
LKIGTSNELNYNMLCFAMTGGPYDYVYSWPIYESLFKPNANGTIDPWLAESVESDAKALTYTIHIRRGVTFSDGSVLNAT
VVKWNLDHYLKVGAKRVALLGSITSVDVLDDYTVRLVLSKWSSFIPAALSRECGYMFSQQHYEKYGDDYCQQHPVGTGPF
VLKEWVRDVSKSFVRNEHYWGGKVLLEAVQYTIYNDSLVGQAALMSDEIDVMIGLGLNGVKTLAANGFDAPIEPLQDHAS
LLVFNSLNTSNDDPMGNLLVRQAISHAINRKSIIEAAFLGYADIASQFGIGSHFVNKDIVGYDYNVAKAKELLAKAGFPQ
GFSTKIQAAASDTNKIVLQIMQADLAKVGIQAKIELLTGAASNKAETGWGSGMWYHTSSVYVDVAMQMSSMFPKGLTGGV
LGLTTMLRPDDVSDALVRAVSSTSDAESVKAVGEANKMMIDTYALYVPIAQYSTIYVLSKRVKASGIGATFFSVASLSTA
YLDT

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=411, Percent_Identity=28.7104622871046, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789966, Length=452, Percent_Identity=28.5398230088496, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789887, Length=348, Percent_Identity=27.2988505747126, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787762, Length=472, Percent_Identity=22.0338983050847, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787551, Length=470, Percent_Identity=24.0425531914894, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61380; Mature: 61249

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPECLQTGEKPSIEGGKKEHFYRSIKSQNAAIAIKKMGNEIVMKTAIKRRNFIKAGASLA
CCCHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHH
AFGLLGDFANQAMAKSVEGGTLKIGTSNELNYNMLCFAMTGGPYDYVYSWPIYESLFKPN
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEECCCCCEEEECHHHHHHHCCC
ANGTIDPWLAESVESDAKALTYTIHIRRGVTFSDGSVLNATVVKWNLDHYLKVGAKRVAL
CCCCCCHHHHHHHHCCCCEEEEEEEEECCEEECCCCEEEEEEEEECHHHHHHHHHHHHHH
LGSITSVDVLDDYTVRLVLSKWSSFIPAALSRECGYMFSQQHYEKYGDDYCQQHPVGTGP
HHCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCH
FVLKEWVRDVSKSFVRNEHYWGGKVLLEAVQYTIYNDSLVGQAALMSDEIDVMIGLGLNG
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCHHCHHHHHCCCCEEEEECCCCH
VKTLAANGFDAPIEPLQDHASLLVFNSLNTSNDDPMGNLLVRQAISHAINRKSIIEAAFL
HHHHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GYADIASQFGIGSHFVNKDIVGYDYNVAKAKELLAKAGFPQGFSTKIQAAASDTNKIVLQ
HHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHH
IMQADLAKVGIQAKIELLTGAASNKAETGWGSGMWYHTSSVYVDVAMQMSSMFPKGLTGG
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHCCCCCCCCC
VLGLTTMLRPDDVSDALVRAVSSTSDAESVKAVGEANKMMIDTYALYVPIAQYSTIYVLS
HHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHEEEEEHHHHHEEEEHH
KRVKASGIGATFFSVASLSTAYLDT
HHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PECLQTGEKPSIEGGKKEHFYRSIKSQNAAIAIKKMGNEIVMKTAIKRRNFIKAGASLA
CCHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHH
AFGLLGDFANQAMAKSVEGGTLKIGTSNELNYNMLCFAMTGGPYDYVYSWPIYESLFKPN
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEECCCCCEEEECHHHHHHHCCC
ANGTIDPWLAESVESDAKALTYTIHIRRGVTFSDGSVLNATVVKWNLDHYLKVGAKRVAL
CCCCCCHHHHHHHHCCCCEEEEEEEEECCEEECCCCEEEEEEEEECHHHHHHHHHHHHHH
LGSITSVDVLDDYTVRLVLSKWSSFIPAALSRECGYMFSQQHYEKYGDDYCQQHPVGTGP
HHCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCH
FVLKEWVRDVSKSFVRNEHYWGGKVLLEAVQYTIYNDSLVGQAALMSDEIDVMIGLGLNG
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCHHCHHHHHCCCCEEEEECCCCH
VKTLAANGFDAPIEPLQDHASLLVFNSLNTSNDDPMGNLLVRQAISHAINRKSIIEAAFL
HHHHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GYADIASQFGIGSHFVNKDIVGYDYNVAKAKELLAKAGFPQGFSTKIQAAASDTNKIVLQ
HHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHH
IMQADLAKVGIQAKIELLTGAASNKAETGWGSGMWYHTSSVYVDVAMQMSSMFPKGLTGG
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHCCCCCCCCC
VLGLTTMLRPDDVSDALVRAVSSTSDAESVKAVGEANKMMIDTYALYVPIAQYSTIYVLS
HHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHEEEEEHHHHHEEEEHH
KRVKASGIGATFFSVASLSTAYLDT
HHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA