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The map label for this gene is prpB [H]

Identifier: 222102799

GI number: 222102799

Start: 462221

End: 463141

Strand: Direct

Name: prpB [H]

Synonym: Avi_7515

Alternate gene names: 222102799

Gene position: 462221-463141 (Clockwise)

Preceding gene: 222102798

Following gene: 222102800

Centisome position: 73.16

GC content: 52.55

Gene sequence:

>921_bases
TTGTCAGAATATTTGTCTGATGAAAATTTGTACTATGTCCCTGAAATATTTGACTGCATGTCAACCAAAGCCGCCGAAAT
AAACGGCTTTAAAATGGTGATGATATCCAGCAGTGACTTCTCCTGTGCTTACACCGGCATTCCCGACCTCAAGCTTCTGA
CGGTTGACGAATATGCTGGTATGGCGGAACGCATTACCAATATGACGGACATGCCCCTGTTCATTGATGCTGACGAGGGG
TTTGGGCGCCCGTTGCAGACATTTCAAGGATGTCGGAGGCTGGCCAAGGCGGGTGCGCAGGCGATCTTGATCACGGATCT
TGCCGCCAATGGCAGGCCGGGATTGTTGCCGATTGAAGAGGCCGTCTACCGGTTCCGCGCAGCCAAGGACGGGCTTGCGG
GTACGGATTGCTTGCTTCTGGCCCGCTGCGACCACAGCGTTGATAACGACTTTGACGAATTTGTAGAGCGATGCAATCGT
TACCTCGAAGTGGGCGCGGACATCATCTGCCCGCTGGAGCTAAATCGCTCGCAGAAGTATGGCAGCAAGATTGCAGCAGC
CAAGAAGGTTGCCGAACACGTCAAAGCACCCTTCTGGTATCCCAATATGGAAAAGGAAAAGGCCGAAGACATCGCTAGCC
TGCGGGAGTACGGCTATAAATTTGCAGGCGTCCATTATGCCTTTCGCGCAGCTATGCTGGCTATTCTGGACGCTGGTCGG
CACGTATTTGAGAGCGGTACCAACGAGTATATCGCAACGGCGCATGATCACACGGGTTACAAGTTTCATTATTCGCCGAT
GAGTGCGTTTTTTAGGGGTGGCGAGTGGGTCGATAGGGAACGCCAATACGTGTCAAACCCTGACGAGGCTTTGGCCGTGC
GCCTTGAAAAACACTTCACAGGTCCGACTGACAAGTTCTGA

Upstream 100 bases:

>100_bases
CCACAATCATCCTGCCGAATGAATTATGAATTTTACCCCACCGTTTAGCAGTACATTCGGGTAAAATCTTACCGGGAGAT
TGTCCGTGTCCTCCAAAAAA

Downstream 100 bases:

>100_bases
CGACATGACAGTGCCTTCGGCTGATTAAAGACCTCTTTCAGCACCGTTACAGGGGTGTTTCCTTCAATGGAGCGGGATTG
AAGGCCGCTTGCGCATGCCT

Product: carboxyphosphonoenolpyruvate phosphonomutase (CPEP Phosphonomutase)

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSEYLSDENLYYVPEIFDCMSTKAAEINGFKMVMISSSDFSCAYTGIPDLKLLTVDEYAGMAERITNMTDMPLFIDADEG
FGRPLQTFQGCRRLAKAGAQAILITDLAANGRPGLLPIEEAVYRFRAAKDGLAGTDCLLLARCDHSVDNDFDEFVERCNR
YLEVGADIICPLELNRSQKYGSKIAAAKKVAEHVKAPFWYPNMEKEKAEDIASLREYGYKFAGVHYAFRAAMLAILDAGR
HVFESGTNEYIATAHDHTGYKFHYSPMSAFFRGGEWVDRERQYVSNPDEALAVRLEKHFTGPTDKF

Sequences:

>Translated_306_residues
MSEYLSDENLYYVPEIFDCMSTKAAEINGFKMVMISSSDFSCAYTGIPDLKLLTVDEYAGMAERITNMTDMPLFIDADEG
FGRPLQTFQGCRRLAKAGAQAILITDLAANGRPGLLPIEEAVYRFRAAKDGLAGTDCLLLARCDHSVDNDFDEFVERCNR
YLEVGADIICPLELNRSQKYGSKIAAAKKVAEHVKAPFWYPNMEKEKAEDIASLREYGYKFAGVHYAFRAAMLAILDAGR
HVFESGTNEYIATAHDHTGYKFHYSPMSAFFRGGEWVDRERQYVSNPDEALAVRLEKHFTGPTDKF
>Mature_305_residues
SEYLSDENLYYVPEIFDCMSTKAAEINGFKMVMISSSDFSCAYTGIPDLKLLTVDEYAGMAERITNMTDMPLFIDADEGF
GRPLQTFQGCRRLAKAGAQAILITDLAANGRPGLLPIEEAVYRFRAAKDGLAGTDCLLLARCDHSVDNDFDEFVERCNRY
LEVGADIICPLELNRSQKYGSKIAAAKKVAEHVKAPFWYPNMEKEKAEDIASLREYGYKFAGVHYAFRAAMLAILDAGRH
VFESGTNEYIATAHDHTGYKFHYSPMSAFFRGGEWVDRERQYVSNPDEALAVRLEKHFTGPTDKF

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=202, Percent_Identity=27.2277227722772, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 34400; Mature: 34269

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLSDENLYYVPEIFDCMSTKAAEINGFKMVMISSSDFSCAYTGIPDLKLLTVDEYAG
CCCCCCCCCEEECHHHHHHHCCCCCCCCCEEEEEEECCCCCEEECCCCCEEEEEEHHHCC
MAERITNMTDMPLFIDADEGFGRPLQTFQGCRRLAKAGAQAILITDLAANGRPGLLPIEE
HHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHH
AVYRFRAAKDGLAGTDCLLLARCDHSVDNDFDEFVERCNRYLEVGADIICPLELNRSQKY
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
GSKIAAAKKVAEHVKAPFWYPNMEKEKAEDIASLREYGYKFAGVHYAFRAAMLAILDAGR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HVFESGTNEYIATAHDHTGYKFHYSPMSAFFRGGEWVDRERQYVSNPDEALAVRLEKHFT
HHHHCCCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
GPTDKF
CCCCCC
>Mature Secondary Structure 
SEYLSDENLYYVPEIFDCMSTKAAEINGFKMVMISSSDFSCAYTGIPDLKLLTVDEYAG
CCCCCCCCEEECHHHHHHHCCCCCCCCCEEEEEEECCCCCEEECCCCCEEEEEEHHHCC
MAERITNMTDMPLFIDADEGFGRPLQTFQGCRRLAKAGAQAILITDLAANGRPGLLPIEE
HHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHH
AVYRFRAAKDGLAGTDCLLLARCDHSVDNDFDEFVERCNRYLEVGADIICPLELNRSQKY
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
GSKIAAAKKVAEHVKAPFWYPNMEKEKAEDIASLREYGYKFAGVHYAFRAAMLAILDAGR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HVFESGTNEYIATAHDHTGYKFHYSPMSAFFRGGEWVDRERQYVSNPDEALAVRLEKHFT
HHHHCCCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
GPTDKF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]