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The map label for this gene is attR [C]

Identifier: 222102672

GI number: 222102672

Start: 317614

End: 319251

Strand: Reverse

Name: attR [C]

Synonym: Avi_7339

Alternate gene names: 222102672

Gene position: 319251-317614 (Counterclockwise)

Preceding gene: 222102673

Following gene: 222102670

Centisome position: 50.53

GC content: 58.61

Gene sequence:

>1638_bases
ATGAGCAGGGATGACCAAGTAAACGAGGAAGAGGCATCCTTGCGCCGCAACCGGCTCAATATCCTCACCTGGGAGCGGAA
TGCGCAGGATATTGAGAGCGCAACCCATCAGGCACGCCTTCGCCAGCTTCAGACCTATGCCGGCGCGACATTTTCTCCGG
GCGCTTATGTGGCGGAAAAGGCTGAAATCCATACGGATAGGCTGGTGATGGGCGCGGGCTCCTGGATTGCCGGCCATGCG
CTGGTGCGTGGCGATGTCGAGCTTGGCGAGAACGTTTCGATCAATGCCTATGCCTGCATGTCTGGCAGGGTACGGGTGGG
AAATGGGGTGCGGATTGCCTCGCATGTCAGCATTATCGGCTTCAATCATGGCTTCGACGATCTCGAAACGCCGATCTACC
GCCAGCCGCTGACATCGCTCGGCATCGAGATTGGTGACGATGTCTGGATTGGTGCCAATGCGGTCATTCTGGATGGCGCC
CGGATCGGCAGCGGTGCCATTATCGCTGCTGGTGCCGTGGTCTCGAAAGACATCCCACCACAGGCCATCGCTGGCGGCGT
ACCGGCCCGCGTGCTGCGGATGCGCGGCAAAGACACGGCGTCTACCCGCAAAACCGAGGTGGTCGAGGCCGCGCTTGCCG
CAATCGATGCTGCCGCTTCAACTGATTGGCGAGCGGTGGTGGAGCGATATCGTATCGGCGATGATTACAGTGCACCGGAT
GCAAGCGGCAAACCCGTTACCAGCATTCGCCATCTCTGCGACGCCATCGAAATTGCCGCTGCCTTCGGTGATATCGGCTC
GATATTCGATGTCGATGCCACCATCGCCCGCCTGCAAGCCGTTCAGGACCCGCAAACCGGCCTTTTCCTCGATCCTTATC
GTGAAACGCAGCAGCCTCTGCATCACGATCCAATTGCCTTGTACAATGTGCTGGCGGTTGGATATGCCCTTGAACGCCTA
GGATCAGCGCCACTCCACCTCTTCCGGTTCATCGAACGCCTGAGTGCGAGCGATCTTGTGCAATGGCTTGACGGGTTGCC
CTGGCAAACGCGCGCCTGGCATTGCGGCGCGACCGTTGACGCCATCGCAACTGCCGTGCATTTCAACGGTCGTTATTTTG
GCGCAGATGCCAATCGGCACAGTTTGTTTGGCTGGCTGAGCCTCAACTGCAATCCAGCATCCGGCCTTTGGGGTACGGTG
ACACCAGAAGACGGGCTGTTGCAGCCGGTCAATGGTTTTTATCGTCTGACGCGCGGCGCTCATGCGCAATTTGGCATACC
GGTTCCATACCCGAACGCCGCCATCGATTCCGTGATCGCCAATTACCGGGCTCATGAAGGATTTCGTGGACAAGGCTTCA
ATGCCTGCAATCTGCTCGACACGATCCACCCGCTGCTCCTTTGCCTGAAGCAGACACCTCATCGCCGCGACGAGGCGATG
GAAATCGCAACTGGCATCATTCTGCAACTGTCTACACGGTGGCAAAGTGGGCAAGGCTTCCCGTTCGCAGACGGTCATCC
GCCCGGGTTGCAGGGAACTGAAATGTGGCTCACGACCTGTTGGCTTTCAGCCAAACTCCTGAACCTCTCCAGCAGCCTGT
CATTCAAGCCACGAGGCATCCATCGGTACCGAGGATGA

Upstream 100 bases:

>100_bases
CTCTTGCCGCTCCGCCTACTTGCCCCGATCTCGAGGTGCTGGAGCAGCAGTTTAGAGACGTTGGACGCCGCGTCGCGGCC
TTCGATATCGGCTAAGCGCC

Downstream 100 bases:

>100_bases
GACAGCATTTTCAAACGCTACCCCCTCGCCAACCTCTTTGCTGCGAGATATCGATTATGTCATTTTCTACGTAATTTGAA
GTGGCCTCCCGGGGCGTATG

Product: transacetylase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSRDDQVNEEEASLRRNRLNILTWERNAQDIESATHQARLRQLQTYAGATFSPGAYVAEKAEIHTDRLVMGAGSWIAGHA
LVRGDVELGENVSINAYACMSGRVRVGNGVRIASHVSIIGFNHGFDDLETPIYRQPLTSLGIEIGDDVWIGANAVILDGA
RIGSGAIIAAGAVVSKDIPPQAIAGGVPARVLRMRGKDTASTRKTEVVEAALAAIDAAASTDWRAVVERYRIGDDYSAPD
ASGKPVTSIRHLCDAIEIAAAFGDIGSIFDVDATIARLQAVQDPQTGLFLDPYRETQQPLHHDPIALYNVLAVGYALERL
GSAPLHLFRFIERLSASDLVQWLDGLPWQTRAWHCGATVDAIATAVHFNGRYFGADANRHSLFGWLSLNCNPASGLWGTV
TPEDGLLQPVNGFYRLTRGAHAQFGIPVPYPNAAIDSVIANYRAHEGFRGQGFNACNLLDTIHPLLLCLKQTPHRRDEAM
EIATGIILQLSTRWQSGQGFPFADGHPPGLQGTEMWLTTCWLSAKLLNLSSSLSFKPRGIHRYRG

Sequences:

>Translated_545_residues
MSRDDQVNEEEASLRRNRLNILTWERNAQDIESATHQARLRQLQTYAGATFSPGAYVAEKAEIHTDRLVMGAGSWIAGHA
LVRGDVELGENVSINAYACMSGRVRVGNGVRIASHVSIIGFNHGFDDLETPIYRQPLTSLGIEIGDDVWIGANAVILDGA
RIGSGAIIAAGAVVSKDIPPQAIAGGVPARVLRMRGKDTASTRKTEVVEAALAAIDAAASTDWRAVVERYRIGDDYSAPD
ASGKPVTSIRHLCDAIEIAAAFGDIGSIFDVDATIARLQAVQDPQTGLFLDPYRETQQPLHHDPIALYNVLAVGYALERL
GSAPLHLFRFIERLSASDLVQWLDGLPWQTRAWHCGATVDAIATAVHFNGRYFGADANRHSLFGWLSLNCNPASGLWGTV
TPEDGLLQPVNGFYRLTRGAHAQFGIPVPYPNAAIDSVIANYRAHEGFRGQGFNACNLLDTIHPLLLCLKQTPHRRDEAM
EIATGIILQLSTRWQSGQGFPFADGHPPGLQGTEMWLTTCWLSAKLLNLSSSLSFKPRGIHRYRG
>Mature_544_residues
SRDDQVNEEEASLRRNRLNILTWERNAQDIESATHQARLRQLQTYAGATFSPGAYVAEKAEIHTDRLVMGAGSWIAGHAL
VRGDVELGENVSINAYACMSGRVRVGNGVRIASHVSIIGFNHGFDDLETPIYRQPLTSLGIEIGDDVWIGANAVILDGAR
IGSGAIIAAGAVVSKDIPPQAIAGGVPARVLRMRGKDTASTRKTEVVEAALAAIDAAASTDWRAVVERYRIGDDYSAPDA
SGKPVTSIRHLCDAIEIAAAFGDIGSIFDVDATIARLQAVQDPQTGLFLDPYRETQQPLHHDPIALYNVLAVGYALERLG
SAPLHLFRFIERLSASDLVQWLDGLPWQTRAWHCGATVDAIATAVHFNGRYFGADANRHSLFGWLSLNCNPASGLWGTVT
PEDGLLQPVNGFYRLTRGAHAQFGIPVPYPNAAIDSVIANYRAHEGFRGQGFNACNLLDTIHPLLLCLKQTPHRRDEAME
IATGIILQLSTRWQSGQGFPFADGHPPGLQGTEMWLTTCWLSAKLLNLSSSLSFKPRGIHRYRG

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=115, Percent_Identity=35.6521739130435, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786664, Length=92, Percent_Identity=39.1304347826087, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788345, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=105, Percent_Identity=39.0476190476191, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 59251; Mature: 59119

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDDQVNEEEASLRRNRLNILTWERNAQDIESATHQARLRQLQTYAGATFSPGAYVAEK
CCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
AEIHTDRLVMGAGSWIAGHALVRGDVELGENVSINAYACMSGRVRVGNGVRIASHVSIIG
HHHCCCEEEEECCCCHHCCEEEECCCCCCCCCEEEEEEEECCCEEECCCEEEEEEEEEEE
FNHGFDDLETPIYRQPLTSLGIEIGDDVWIGANAVILDGARIGSGAIIAAGAVVSKDIPP
ECCCCHHHCCCHHHCCHHHCCCEECCCEEECCCEEEEECCEECCCCEEEECHHHCCCCCH
QAIAGGVPARVLRMRGKDTASTRKTEVVEAALAAIDAAASTDWRAVVERYRIGDDYSAPD
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
ASGKPVTSIRHLCDAIEIAAAFGDIGSIFDVDATIARLQAVQDPQTGLFLDPYRETQQPL
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHCCCC
HHDPIALYNVLAVGYALERLGSAPLHLFRFIERLSASDLVQWLDGLPWQTRAWHCGATVD
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEECCCHHH
AIATAVHFNGRYFGADANRHSLFGWLSLNCNPASGLWGTVTPEDGLLQPVNGFYRLTRGA
HHHHHEEECCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
HAQFGIPVPYPNAAIDSVIANYRAHEGFRGQGFNACNLLDTIHPLLLCLKQTPHRRDEAM
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHH
EIATGIILQLSTRWQSGQGFPFADGHPPGLQGTEMWLTTCWLSAKLLNLSSSLSFKPRGI
HHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HRYRG
CCCCC
>Mature Secondary Structure 
SRDDQVNEEEASLRRNRLNILTWERNAQDIESATHQARLRQLQTYAGATFSPGAYVAEK
CCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
AEIHTDRLVMGAGSWIAGHALVRGDVELGENVSINAYACMSGRVRVGNGVRIASHVSIIG
HHHCCCEEEEECCCCHHCCEEEECCCCCCCCCEEEEEEEECCCEEECCCEEEEEEEEEEE
FNHGFDDLETPIYRQPLTSLGIEIGDDVWIGANAVILDGARIGSGAIIAAGAVVSKDIPP
ECCCCHHHCCCHHHCCHHHCCCEECCCEEECCCEEEEECCEECCCCEEEECHHHCCCCCH
QAIAGGVPARVLRMRGKDTASTRKTEVVEAALAAIDAAASTDWRAVVERYRIGDDYSAPD
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
ASGKPVTSIRHLCDAIEIAAAFGDIGSIFDVDATIARLQAVQDPQTGLFLDPYRETQQPL
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHCCCC
HHDPIALYNVLAVGYALERLGSAPLHLFRFIERLSASDLVQWLDGLPWQTRAWHCGATVD
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEECCCHHH
AIATAVHFNGRYFGADANRHSLFGWLSLNCNPASGLWGTVTPEDGLLQPVNGFYRLTRGA
HHHHHEEECCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
HAQFGIPVPYPNAAIDSVIANYRAHEGFRGQGFNACNLLDTIHPLLLCLKQTPHRRDEAM
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHH
EIATGIILQLSTRWQSGQGFPFADGHPPGLQGTEMWLTTCWLSAKLLNLSSSLSFKPRGI
HHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HRYRG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]