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The map label for this gene is mcyE [H]

Identifier: 222102646

GI number: 222102646

Start: 269252

End: 270652

Strand: Reverse

Name: mcyE [H]

Synonym: Avi_7303

Alternate gene names: 222102646

Gene position: 270652-269252 (Counterclockwise)

Preceding gene: 222102647

Following gene: 222102645

Centisome position: 42.84

GC content: 61.1

Gene sequence:

>1401_bases
ATGAATGCGTTGACGAGCAATCCGACGCCCGCCCAAGCGGCAGCCGCCATCATAGCCGCTTTCAATATGAAGACGGAAAA
GTCGAAGGTGGCAAGTGCGCAAAACCGGCGCGTGCTCTCTGATAAATCGGCAATCGGCGTTCCCTTCTCGCTGATGGCCA
AGGAGGCACTCTACCCGATTGTGGTAGACCGGGCGGAGGGAACAGTACTGCATGATATCGACGGCAATCGCTATATCGAT
GTGCTGATGGGGATGGGCATCAACCTCTTTGGTCACAATCCGCCCTTTATCCGTGACGCGATTGAGGCACAGCTTGCCAA
GGGCTTTGCGCTTGGGCCGCAATCTGATCTGGCAGGCGAGACCGCAGCGCTCTTCTGCAAGCTGACGGGCAAGGAAAGAG
TAACCTTCACCAATACCGGCACCGAAGCCGTGATGACCGCGCTGCGGCTGGCGCGGGCGGCGACCGGGCGGCACAAAATT
GCGATGTTCATCGGCTCCTATCACGGCCATTCGGATCAGGTTCTGAACCGCATTGCCGCCCCCGATGACGAGCGCACCGT
TCCCGCCTTTCCCGGCATTTCGCCTGCAACGGCGGAGGATGTGGTGTTGCTCCCCTATAACGACCCGCGCGCACTGGACA
TCTTGGAGCGGGATGGCGAAACCTTTGCCGCCGTGCTGGTTGAGCCGGTGCAAAGTCGCAATATCGACGTCCAGCCCCGC
GCCTTTCTGCATGCGCTGCGTGATGTGACGCAGCGGACGGGTGCTGTCCTCATTTTCGACGAGATGATCAGCGGCTTTCG
CGTCGCACCCGGCGGCGCGCAGCAACATTTCGAAGTCGAGGCGGATCTGGCGACCTATGGCAAGATTGCTGGCGGCGGAC
TGCCGCTGGCGTTGATTGCGGGCAGCAATCGGTTGATGAACCATATCGACGGTGGCCCCTGGTCTTTCGGGGATGAGTCT
GTTCCCCCAGCCCAACCGACCTTTTTTGCCGGAACCTATTGCCGCCACCCCTTGGCGCTTGCAGCCGCGCGCGCAGCCGC
GACGTACATGCTCCAGCAAGGCAGGTCTCTTCAGGATGACCTCAACGCACGCACGCGCAGCCTTGTCGAGCGCCTCAATG
TCTCGCTTGCGGCAGCGCGGCTGCCCGTTGTCTTCACCCAGTTCGGCTCCTTCTTCTCGATTGCGGTCAACCGCAGCCGC
ATTCCGCCGCTTGCACTGGGATTGCTGTCGCTCGAACTGCTCACCGCTGGCATTCATCTGCGCAGCGGCGACAAGGGCGG
CTTCCTTTCCACCGCCCATTCGGATGGCGACATCACAGCCATCCACGATGCCTTCCTGAACGGCCTGCAATCGCTTGCAG
GTTTCGGCCTCATCCCCCTCTCCGACGGAACAACGCCATGA

Upstream 100 bases:

>100_bases
CGCAAGGCGGGCCGGTCGATGACAGCCATGCCGCCTATTTCTTCTGCCGGAAAGCGCCCACTGCCGAAAAGCAGGCAGAG
ACCGCAAAGGAAACCGCCCT

Downstream 100 bases:

>100_bases
GCAACCAGGACCAAACCATTGACTTTCCGCACCGCGTCGTCATCAGCGACGAGGAGCGATACTCGATCTGGCCGACCTAC
AAGCCCATCCCCCTTGGCTG

Product: polyketide synthase and peptide synthetase

Products: 5-Amino-4-oxopentanoate [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNALTSNPTPAQAAAAIIAAFNMKTEKSKVASAQNRRVLSDKSAIGVPFSLMAKEALYPIVVDRAEGTVLHDIDGNRYID
VLMGMGINLFGHNPPFIRDAIEAQLAKGFALGPQSDLAGETAALFCKLTGKERVTFTNTGTEAVMTALRLARAATGRHKI
AMFIGSYHGHSDQVLNRIAAPDDERTVPAFPGISPATAEDVVLLPYNDPRALDILERDGETFAAVLVEPVQSRNIDVQPR
AFLHALRDVTQRTGAVLIFDEMISGFRVAPGGAQQHFEVEADLATYGKIAGGGLPLALIAGSNRLMNHIDGGPWSFGDES
VPPAQPTFFAGTYCRHPLALAAARAAATYMLQQGRSLQDDLNARTRSLVERLNVSLAAARLPVVFTQFGSFFSIAVNRSR
IPPLALGLLSLELLTAGIHLRSGDKGGFLSTAHSDGDITAIHDAFLNGLQSLAGFGLIPLSDGTTP

Sequences:

>Translated_466_residues
MNALTSNPTPAQAAAAIIAAFNMKTEKSKVASAQNRRVLSDKSAIGVPFSLMAKEALYPIVVDRAEGTVLHDIDGNRYID
VLMGMGINLFGHNPPFIRDAIEAQLAKGFALGPQSDLAGETAALFCKLTGKERVTFTNTGTEAVMTALRLARAATGRHKI
AMFIGSYHGHSDQVLNRIAAPDDERTVPAFPGISPATAEDVVLLPYNDPRALDILERDGETFAAVLVEPVQSRNIDVQPR
AFLHALRDVTQRTGAVLIFDEMISGFRVAPGGAQQHFEVEADLATYGKIAGGGLPLALIAGSNRLMNHIDGGPWSFGDES
VPPAQPTFFAGTYCRHPLALAAARAAATYMLQQGRSLQDDLNARTRSLVERLNVSLAAARLPVVFTQFGSFFSIAVNRSR
IPPLALGLLSLELLTAGIHLRSGDKGGFLSTAHSDGDITAIHDAFLNGLQSLAGFGLIPLSDGTTP
>Mature_466_residues
MNALTSNPTPAQAAAAIIAAFNMKTEKSKVASAQNRRVLSDKSAIGVPFSLMAKEALYPIVVDRAEGTVLHDIDGNRYID
VLMGMGINLFGHNPPFIRDAIEAQLAKGFALGPQSDLAGETAALFCKLTGKERVTFTNTGTEAVMTALRLARAATGRHKI
AMFIGSYHGHSDQVLNRIAAPDDERTVPAFPGISPATAEDVVLLPYNDPRALDILERDGETFAAVLVEPVQSRNIDVQPR
AFLHALRDVTQRTGAVLIFDEMISGFRVAPGGAQQHFEVEADLATYGKIAGGGLPLALIAGSNRLMNHIDGGPWSFGDES
VPPAQPTFFAGTYCRHPLALAAARAAATYMLQQGRSLQDDLNARTRSLVERLNVSLAAARLPVVFTQFGSFFSIAVNRSR
IPPLALGLLSLELLTAGIHLRSGDKGGFLSTAHSDGDITAIHDAFLNGLQSLAGFGLIPLSDGTTP

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=309, Percent_Identity=28.4789644012945, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=400, Percent_Identity=32.5, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1787560, Length=297, Percent_Identity=29.2929292929293, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788044, Length=260, Percent_Identity=34.2307692307692, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789016, Length=346, Percent_Identity=29.1907514450867, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789759, Length=323, Percent_Identity=28.7925696594427, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693181, Length=297, Percent_Identity=29.2929292929293, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=316, Percent_Identity=25.3164556962025, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=269, Percent_Identity=27.8810408921933, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21357415, Length=265, Percent_Identity=27.9245283018868, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 5.4.3.8 [C]

Molecular weight: Translated: 49557; Mature: 49557

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALTSNPTPAQAAAAIIAAFNMKTEKSKVASAQNRRVLSDKSAIGVPFSLMAKEALYPI
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCEE
VVDRAEGTVLHDIDGNRYIDVLMGMGINLFGHNPPFIRDAIEAQLAKGFALGPQSDLAGE
EEECCCCCEEEECCCCCEEHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
TAALFCKLTGKERVTFTNTGTEAVMTALRLARAATGRHKIAMFIGSYHGHSDQVLNRIAA
CEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCC
PDDERTVPAFPGISPATAEDVVLLPYNDPRALDILERDGETFAAVLVEPVQSRNIDVQPR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCEEEHHHHHHHHHCCCCCCHH
AFLHALRDVTQRTGAVLIFDEMISGFRVAPGGAQQHFEVEADLATYGKIAGGGLPLALIA
HHHHHHHHHHHHCCEEEEEHHHHCCEEECCCCCCCCEEEEECHHHHCCCCCCCCCEEEEE
GSNRLMNHIDGGPWSFGDESVPPAQPTFFAGTYCRHPLALAAARAAATYMLQQGRSLQDD
CCCHHHHHCCCCCCCCCCCCCCCCCCCEECCHHHCCHHHHHHHHHHHHHHHHCCCCHHHH
LNARTRSLVERLNVSLAAARLPVVFTQFGSFFSIAVNRSRIPPLALGLLSLELLTAGIHL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEE
RSGDKGGFLSTAHSDGDITAIHDAFLNGLQSLAGFGLIPLSDGTTP
ECCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCCCC
>Mature Secondary Structure
MNALTSNPTPAQAAAAIIAAFNMKTEKSKVASAQNRRVLSDKSAIGVPFSLMAKEALYPI
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCEE
VVDRAEGTVLHDIDGNRYIDVLMGMGINLFGHNPPFIRDAIEAQLAKGFALGPQSDLAGE
EEECCCCCEEEECCCCCEEHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
TAALFCKLTGKERVTFTNTGTEAVMTALRLARAATGRHKIAMFIGSYHGHSDQVLNRIAA
CEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCC
PDDERTVPAFPGISPATAEDVVLLPYNDPRALDILERDGETFAAVLVEPVQSRNIDVQPR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCEEEHHHHHHHHHCCCCCCHH
AFLHALRDVTQRTGAVLIFDEMISGFRVAPGGAQQHFEVEADLATYGKIAGGGLPLALIA
HHHHHHHHHHHHCCEEEEEHHHHCCEEECCCCCCCCEEEEECHHHHCCCCCCCCCEEEEE
GSNRLMNHIDGGPWSFGDESVPPAQPTFFAGTYCRHPLALAAARAAATYMLQQGRSLQDD
CCCHHHHHCCCCCCCCCCCCCCCCCCCEECCHHHCCHHHHHHHHHHHHHHHHCCCCHHHH
LNARTRSLVERLNVSLAAARLPVVFTQFGSFFSIAVNRSRIPPLALGLLSLELLTAGIHL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEE
RSGDKGGFLSTAHSDGDITAIHDAFLNGLQSLAGFGLIPLSDGTTP
ECCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ethanolamine; Methylamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Glutamate 1-semialdehyde [C]

Specific reaction: L-Glutamate 1-semialdehyde --> 5-Amino-4-oxopentanoate [C]

General reaction: Group transfer (intramolecular amino group isomerization) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]