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The map label for this gene is opuAB [H]

Identifier: 222102603

GI number: 222102603

Start: 210134

End: 210982

Strand: Reverse

Name: opuAB [H]

Synonym: Avi_7253

Alternate gene names: 222102603

Gene position: 210982-210134 (Counterclockwise)

Preceding gene: 222102604

Following gene: 222102602

Centisome position: 33.4

GC content: 55.24

Gene sequence:

>849_bases
ATGGACACCTCCGTTATTACAGACAGCTTCGATGAACACATTGACGACACGCTCAACTGGATCAGCGATCATGCCTCATG
GCTGTTTGATTCCATCAGAGCGGTGCTGGAAGGCACCTATGGCGGCGTTCTCTGGCTGTTTCAGCTTGCGCCATTCTATG
TGGTTGCCTTGATTGCAGCCCTTCTCGGATGGCGGTTGATCAACAGCAAGGCCGGTCTTTTGATCGGTGTCGCCATGCTG
GGCTGCACAGTCATGGGCCTTTGGGCCGAAACCATGAGCACTTTGGCGTTGGTCATCACAGCCACCTTCATGGCGCTGGT
GATCGGCATTCCCGTTGGTATCGTGGCGGGTTTCGTCCAAGCCTTCGACAAGATTGTCGAGCCGGTGCTCGATCTCATCC
AGACACTGCCACCTTATATCTACCTGCTGCCAACCATTGCACTGATGGGATATGGACCTGCCACGGCCCTTCTTGCCACG
GTCATTGTCGCCATGCCACCGGCGATCCGGCTGACATCTCTCGGCATCCGCAGAACGCCCAATGATTTTATTGAACTGGG
ACAGGCCAACGGCCTGACGTCCTGGCAGATGTTCGTCAAGATCAGACTGCCATTCGCGATACCGAGCGTCATGGCCGGGA
TTAACCAGAGCCTGATGATGGCGTTCGGCATGGTGGTGATCGCTGGCATCGTCGGTTCTGGCGGGCTTGGTGAAACCATC
TATAGCGCCGTCAGAACGCTGAATATCGCAACGTCGATCAACGCCGCAATTGCCATTGTCATTCTCACCATGGTGCTTGA
TCGACTGACACAAAGCGCGGCGAACCGTAACACGGGAGTAAAATCATGA

Upstream 100 bases:

>100_bases
GAGGACGGCAACGTCATCGGCATTGTCACCACGCGCGGCCTGCTCTGCGGTGTCGCAGGCAGTCCGGCCCAGCCGACCGC
AGCGGCATAGGGGGAATGTC

Downstream 100 bases:

>100_bases
CTCCAGAAACAGTTTGGTTTTCGCCGGGTGCCTATCTCGCTCCCGCCGTCGATTGGTTGAACGCGAATTTCCATCCCTTC
TTCAACACTGTGACAAAAAT

Product: ABC transporter

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDTSVITDSFDEHIDDTLNWISDHASWLFDSIRAVLEGTYGGVLWLFQLAPFYVVALIAALLGWRLINSKAGLLIGVAML
GCTVMGLWAETMSTLALVITATFMALVIGIPVGIVAGFVQAFDKIVEPVLDLIQTLPPYIYLLPTIALMGYGPATALLAT
VIVAMPPAIRLTSLGIRRTPNDFIELGQANGLTSWQMFVKIRLPFAIPSVMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YSAVRTLNIATSINAAIAIVILTMVLDRLTQSAANRNTGVKS

Sequences:

>Translated_282_residues
MDTSVITDSFDEHIDDTLNWISDHASWLFDSIRAVLEGTYGGVLWLFQLAPFYVVALIAALLGWRLINSKAGLLIGVAML
GCTVMGLWAETMSTLALVITATFMALVIGIPVGIVAGFVQAFDKIVEPVLDLIQTLPPYIYLLPTIALMGYGPATALLAT
VIVAMPPAIRLTSLGIRRTPNDFIELGQANGLTSWQMFVKIRLPFAIPSVMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YSAVRTLNIATSINAAIAIVILTMVLDRLTQSAANRNTGVKS
>Mature_282_residues
MDTSVITDSFDEHIDDTLNWISDHASWLFDSIRAVLEGTYGGVLWLFQLAPFYVVALIAALLGWRLINSKAGLLIGVAML
GCTVMGLWAETMSTLALVITATFMALVIGIPVGIVAGFVQAFDKIVEPVLDLIQTLPPYIYLLPTIALMGYGPATALLAT
VIVAMPPAIRLTSLGIRRTPNDFIELGQANGLTSWQMFVKIRLPFAIPSVMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YSAVRTLNIATSINAAIAIVILTMVLDRLTQSAANRNTGVKS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=275, Percent_Identity=35.6363636363636, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788449, Length=185, Percent_Identity=26.4864864864865, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30073; Mature: 30073

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSVITDSFDEHIDDTLNWISDHASWLFDSIRAVLEGTYGGVLWLFQLAPFYVVALIAA
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LLGWRLINSKAGLLIGVAMLGCTVMGLWAETMSTLALVITATFMALVIGIPVGIVAGFVQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDKIVEPVLDLIQTLPPYIYLLPTIALMGYGPATALLATVIVAMPPAIRLTSLGIRRTP
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCC
NDFIELGQANGLTSWQMFVKIRLPFAIPSVMAGINQSLMMAFGMVVIAGIVGSGGLGETI
HHHHHHCCCCCCHHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
YSAVRTLNIATSINAAIAIVILTMVLDRLTQSAANRNTGVKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDTSVITDSFDEHIDDTLNWISDHASWLFDSIRAVLEGTYGGVLWLFQLAPFYVVALIAA
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LLGWRLINSKAGLLIGVAMLGCTVMGLWAETMSTLALVITATFMALVIGIPVGIVAGFVQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDKIVEPVLDLIQTLPPYIYLLPTIALMGYGPATALLATVIVAMPPAIRLTSLGIRRTP
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCC
NDFIELGQANGLTSWQMFVKIRLPFAIPSVMAGINQSLMMAFGMVVIAGIVGSGGLGETI
HHHHHHCCCCCCHHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
YSAVRTLNIATSINAAIAIVILTMVLDRLTQSAANRNTGVKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]