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The map label for this gene is opuAB [H]

Identifier: 222102602

GI number: 222102602

Start: 209280

End: 210137

Strand: Reverse

Name: opuAB [H]

Synonym: Avi_7252

Alternate gene names: 222102602

Gene position: 210137-209280 (Counterclockwise)

Preceding gene: 222102603

Following gene: 222102601

Centisome position: 33.26

GC content: 58.04

Gene sequence:

>858_bases
ATGACTCCAGAAACAGTTTGGTTTTCGCCGGGTGCCTATCTCGCTCCCGCCGTCGATTGGTTGAACGCGAATTTCCATCC
CTTCTTCAACACTGTGACAAAAATCATTGAAGCCGTTCTTGGAAGTATCGAAACCATCCTTCTCTTTCTGCCTTTCTATG
CCGTCATTCTGATCACCGTTGCTCTGGCTGCCGTCTTCGTCACTCTTCGTGTTGCGGTAACAAGTGCGTTTGCCTTGTCA
TTCTGCTTCCTGTCGGGTCTATGGGAAGCGTCGATGCAGACTCTGGCTCTGGTGACGGTGTCGGTTTGTATCTCCGTGCT
GATTGCCTTTCCGCTTGGCATTCTGGCGTCCCGGCATCGACGGGTCGAGACGATCATTCGCCCTTTGCTCGACATCATGC
AGACGGTGCCACCTTGGGTCTATCTGATCCCCGCCGTGATGATTTTCAGCCTCGGGCGGGTCCCGGCGATCATCGCGACG
ATCGTCTACGGCATACCACCGATGTTGCGGCTCACGACGCTGGCGTTCAATCAGGTGCCCAAAGACTTGCTGGAACTTGG
TCAGGCGACCGGTGCGTCGCCGCGCGCCATCCTGTTCAAGATTGAAATTCCTGCGGCAACGCCCACGCTGCTCGTAGGGT
TGAATCAGTGCATTCTTCTGTCGCTGGCCATCGTTGTTCTGGCCGGTCTTGTCGGGGCTGGCGGCTTGGGAGCCGAGGTC
ACACGCGGACTGACCCGCATGGAAATGGGTCTCGGCCTGCGGGCAGGATTGGCGATCGTCGCGGTGGCGATTTTCCTCGA
TCGACTGTCACGCGGCGCATTGCAGCGAGGCCGGGCACCTGGCGCAGCCAGCACCTGA

Upstream 100 bases:

>100_bases
TCGCAACGTCGATCAACGCCGCAATTGCCATTGTCATTCTCACCATGGTGCTTGATCGACTGACACAAAGCGCGGCGAAC
CGTAACACGGGAGTAAAATC

Downstream 100 bases:

>100_bases
CACTTGATAAGGAACGATCATGCGGCACAGCTTCTTTTGTATCGACAGCCATACATGCGGCAATCCTGTCCGCCTCGTTG
CCGGGGGCGGACCGCTTCTG

Product: ABC transporter membrane spanning protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTPETVWFSPGAYLAPAVDWLNANFHPFFNTVTKIIEAVLGSIETILLFLPFYAVILITVALAAVFVTLRVAVTSAFALS
FCFLSGLWEASMQTLALVTVSVCISVLIAFPLGILASRHRRVETIIRPLLDIMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKDLLELGQATGASPRAILFKIEIPAATPTLLVGLNQCILLSLAIVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVAIFLDRLSRGALQRGRAPGAAST

Sequences:

>Translated_285_residues
MTPETVWFSPGAYLAPAVDWLNANFHPFFNTVTKIIEAVLGSIETILLFLPFYAVILITVALAAVFVTLRVAVTSAFALS
FCFLSGLWEASMQTLALVTVSVCISVLIAFPLGILASRHRRVETIIRPLLDIMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKDLLELGQATGASPRAILFKIEIPAATPTLLVGLNQCILLSLAIVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVAIFLDRLSRGALQRGRAPGAAST
>Mature_284_residues
TPETVWFSPGAYLAPAVDWLNANFHPFFNTVTKIIEAVLGSIETILLFLPFYAVILITVALAAVFVTLRVAVTSAFALSF
CFLSGLWEASMQTLALVTVSVCISVLIAFPLGILASRHRRVETIIRPLLDIMQTVPPWVYLIPAVMIFSLGRVPAIIATI
VYGIPPMLRLTTLAFNQVPKDLLELGQATGASPRAILFKIEIPAATPTLLVGLNQCILLSLAIVVLAGLVGAGGLGAEVT
RGLTRMEMGLGLRAGLAIVAVAIFLDRLSRGALQRGRAPGAAST

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=270, Percent_Identity=39.6296296296296, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788451, Length=200, Percent_Identity=29.5, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788449, Length=177, Percent_Identity=31.0734463276836, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30317; Mature: 30186

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPETVWFSPGAYLAPAVDWLNANFHPFFNTVTKIIEAVLGSIETILLFLPFYAVILITV
CCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAVFVTLRVAVTSAFALSFCFLSGLWEASMQTLALVTVSVCISVLIAFPLGILASRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVETIIRPLLDIMQTVPPWVYLIPAVMIFSLGRVPAIIATIVYGIPPMLRLTTLAFNQVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
KDLLELGQATGASPRAILFKIEIPAATPTLLVGLNQCILLSLAIVVLAGLVGAGGLGAEV
HHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRGLTRMEMGLGLRAGLAIVAVAIFLDRLSRGALQRGRAPGAAST
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPETVWFSPGAYLAPAVDWLNANFHPFFNTVTKIIEAVLGSIETILLFLPFYAVILITV
CCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAVFVTLRVAVTSAFALSFCFLSGLWEASMQTLALVTVSVCISVLIAFPLGILASRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVETIIRPLLDIMQTVPPWVYLIPAVMIFSLGRVPAIIATIVYGIPPMLRLTTLAFNQVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
KDLLELGQATGASPRAILFKIEIPAATPTLLVGLNQCILLSLAIVVLAGLVGAGGLGAEV
HHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRGLTRMEMGLGLRAGLAIVAVAIFLDRLSRGALQRGRAPGAAST
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]