Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 222102484

GI number: 222102484

Start: 81387

End: 82346

Strand: Direct

Name: braE [H]

Synonym: Avi_7091

Alternate gene names: 222102484

Gene position: 81387-82346 (Clockwise)

Preceding gene: 222102483

Following gene: 222102485

Centisome position: 12.88

GC content: 56.67

Gene sequence:

>960_bases
ATGAAACTGCTTGTTCAGAGCATGGCTCTGGTTGCCGTTATCGGTTCGGTCGCTTTTTTCGCCAGCGGATATATCGATCC
CGGATTGCTCTATCTGCTCTCTGAGGCCCTGCTTTTGCTGTTCATGGCGCAGATGTGGAACCTCCTTGCCGGATATGGCG
GCATGGTCTCGATGGGTCATCAGGCGTTTGTCGGGCTTGGAGCCTATCTGTTTTTCGCCTTGGCCAATTTTGGCGTTTTG
CCAGTGGGCGCTGCCTTGCCGGTGGCCGCCCTTGCTGTAGGGCTGATGGCCCTGCCTTTAGGCCTGCTGCTCTTTCGGCT
GCGCAACGCCTATTTCTCGGTGGCGATCTGGGTTGTGGCCGAAATTCTTGTCATCCTGTTCAGCAAATTTAGTGCGCTGG
GCGGAACGGGGGGCATGACACTGCGCCCCGACGGGGGCAATCTTGACCTGCCGCTTGAGCAATATGTCTTTGCCATTGCG
CTGTTTAGCTGCGTGGGCTTCACGCTGGCCATGCGCTGGGTGCTGGCCAGACCGACAGGCCTTGCCATTCTGGCGGTGCG
CGATAACGAGGATGCGGCACAGGCCGCTGGCGTGAATGTGCAAAAACTCCATCTGGCCCTGTTTGCCATCTCGGCGGCGG
GCTGTGCCTGGGCGGGCAGCCTTTACTACGCCAATGTTCTTTATCTGACACCCACGGATGCCTTTCAGATCAACTGGGTG
ATCGGAATGATGTTCATTGCCGTGGTCGGCGGAATTGGAACTCTCACAGGGCCTGTGATTGGCACGGTGATTTTCATCGG
CTTGCGCGAGTTTCTCACCAGTTTCGGCTTCTCCGGCAGCATCTATTGGATTGTCATGGGGCTGATTGTGATTGTCACCC
TTGTTTATGCACCGCGTGGCCTTTGGCCTGCGCTGCACTCTCTCTTCAGACGAATGAGCATTGAAAGGAAATCCGCATGA

Upstream 100 bases:

>100_bases
AGGTTGTTACCGCCCGTTTCGATGGCAACGCGGGCCTGCTTTATGTCCACCTGACGTTTCTTGCAGGTCTTGGTCTGCGT
GCTTTCCGGGGGAAACTCTC

Downstream 100 bases:

>100_bases
ATGCGTCGAACGATCCCACAAAATGGACGGCATCGGAGGCCAGTCTTGCCCTTGCCGCTAAACAGATCGGCGCTGTGGAG
CTTCTGGATGCCTATCTTGC

Product: ABC transporter nucleotide binding/ATPase protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLLVQSMALVAVIGSVAFFASGYIDPGLLYLLSEALLLLFMAQMWNLLAGYGGMVSMGHQAFVGLGAYLFFALANFGVL
PVGAALPVAALAVGLMALPLGLLLFRLRNAYFSVAIWVVAEILVILFSKFSALGGTGGMTLRPDGGNLDLPLEQYVFAIA
LFSCVGFTLAMRWVLARPTGLAILAVRDNEDAAQAAGVNVQKLHLALFAISAAGCAWAGSLYYANVLYLTPTDAFQINWV
IGMMFIAVVGGIGTLTGPVIGTVIFIGLREFLTSFGFSGSIYWIVMGLIVIVTLVYAPRGLWPALHSLFRRMSIERKSA

Sequences:

>Translated_319_residues
MKLLVQSMALVAVIGSVAFFASGYIDPGLLYLLSEALLLLFMAQMWNLLAGYGGMVSMGHQAFVGLGAYLFFALANFGVL
PVGAALPVAALAVGLMALPLGLLLFRLRNAYFSVAIWVVAEILVILFSKFSALGGTGGMTLRPDGGNLDLPLEQYVFAIA
LFSCVGFTLAMRWVLARPTGLAILAVRDNEDAAQAAGVNVQKLHLALFAISAAGCAWAGSLYYANVLYLTPTDAFQINWV
IGMMFIAVVGGIGTLTGPVIGTVIFIGLREFLTSFGFSGSIYWIVMGLIVIVTLVYAPRGLWPALHSLFRRMSIERKSA
>Mature_319_residues
MKLLVQSMALVAVIGSVAFFASGYIDPGLLYLLSEALLLLFMAQMWNLLAGYGGMVSMGHQAFVGLGAYLFFALANFGVL
PVGAALPVAALAVGLMALPLGLLLFRLRNAYFSVAIWVVAEILVILFSKFSALGGTGGMTLRPDGGNLDLPLEQYVFAIA
LFSCVGFTLAMRWVLARPTGLAILAVRDNEDAAQAAGVNVQKLHLALFAISAAGCAWAGSLYYANVLYLTPTDAFQINWV
IGMMFIAVVGGIGTLTGPVIGTVIFIGLREFLTSFGFSGSIYWIVMGLIVIVTLVYAPRGLWPALHSLFRRMSIERKSA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=284, Percent_Identity=23.5915492957746, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34019; Mature: 34019

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVQSMALVAVIGSVAFFASGYIDPGLLYLLSEALLLLFMAQMWNLLAGYGGMVSMGH
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCH
QAFVGLGAYLFFALANFGVLPVGAALPVAALAVGLMALPLGLLLFRLRNAYFSVAIWVVA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILVILFSKFSALGGTGGMTLRPDGGNLDLPLEQYVFAIALFSCVGFTLAMRWVLARPTG
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LAILAVRDNEDAAQAAGVNVQKLHLALFAISAAGCAWAGSLYYANVLYLTPTDAFQINWV
EEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEHHHH
IGMMFIAVVGGIGTLTGPVIGTVIFIGLREFLTSFGFSGSIYWIVMGLIVIVTLVYAPRG
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
LWPALHSLFRRMSIERKSA
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLLVQSMALVAVIGSVAFFASGYIDPGLLYLLSEALLLLFMAQMWNLLAGYGGMVSMGH
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCH
QAFVGLGAYLFFALANFGVLPVGAALPVAALAVGLMALPLGLLLFRLRNAYFSVAIWVVA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILVILFSKFSALGGTGGMTLRPDGGNLDLPLEQYVFAIALFSCVGFTLAMRWVLARPTG
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LAILAVRDNEDAAQAAGVNVQKLHLALFAISAAGCAWAGSLYYANVLYLTPTDAFQINWV
EEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEHHHH
IGMMFIAVVGGIGTLTGPVIGTVIFIGLREFLTSFGFSGSIYWIVMGLIVIVTLVYAPRG
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
LWPALHSLFRRMSIERKSA
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]