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The map label for this gene is rnhB [H]

Identifier: 222097282

GI number: 222097282

Start: 3585764

End: 3586537

Strand: Reverse

Name: rnhB [H]

Synonym: BCQ_3622

Alternate gene names: 222097282

Gene position: 3586537-3585764 (Counterclockwise)

Preceding gene: 222097283

Following gene: 222097281

Centisome position: 68.78

GC content: 36.56

Gene sequence:

>774_bases
ATGCAAAAAGTGACTATTCAAGAGGCGGAACATCTACTACAAGAAATTATGAGTGAAGAGGACGACCGCTTTCAAATATT
AATAAAAGATGAACGTAAAGGGGTACAAAAGCTCATTTCTAAGTGGTATAAACAAAAAGAGTTAGCGCAAAAGGAAAAAG
AAAAATTTCTAGAAATGTCTAAGTATGAAAATGCGTTACGTGAAAAAGGTCTCACATATATCGCAGGTATAGATGAAGTA
GGTCGTGGACCGTTAGCTGGGCCTGTTGTGACGGCAGCTGTCGTTCTTCCTGAGGATTTTTATATTCCGGGGTTAAATGA
CTCGAAAAAGTTGAGTGAAGCGAAACGTGAGCGTTTTTATGATGAAATTAAAGCGCAAGCAATTGCAATTGGAGTTGGAA
TTGTATCACCGCAAGTTATTGATGAAATAAATATTTATCAAGCGACGAAACAAGCGATGTTAGATGCTGTTGCGAATTTA
TCTTGTACACCACAATATTTATTAATTGATGCGATGAAACTCCCTACACCAATTCCGCAAACATCTATTATTAAAGGTGA
TGCGAAAAGTATTTCTATTTCAGCTGCATCCATTATTGCGAAAGTAACGAGAGATCGTATGATGAAAGAGCTTGGAGAAA
AGTATCCTGCATATGGATTTGAACAACATATGGGATATGGCACAAAACAACATTTAGAAGCGATTGAAGCGCATGGCATA
TTAGAAGAACATCGAAAATCGTTTGCGCCAATTAAAGATATGATTCAAAAATAA

Upstream 100 bases:

>100_bases
GAGTTTGCGGAGCAGACAGAAGATGCAGAAAAAGTAGAAGAAGTATAAGACGTGCGTTTGTACGTCTTTTCTTTTTTTGT
ATATAAAGGGAGTGGCATGA

Downstream 100 bases:

>100_bases
GCTGCGAAAACGCAGCTTATTTTGTATTTTGACATATAAACATTACTATTTTCCAAAAAGAATAAAGCGATTTCAGTTTT
CTGACACTTTATTGTAGACA

Product: ribonuclease hii

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQKVTIQEAEHLLQEIMSEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMSKYENALREKGLTYIAGIDEV
GRGPLAGPVVTAAVVLPEDFYIPGLNDSKKLSEAKRERFYDEIKAQAIAIGVGIVSPQVIDEINIYQATKQAMLDAVANL
SCTPQYLLIDAMKLPTPIPQTSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGI
LEEHRKSFAPIKDMIQK

Sequences:

>Translated_257_residues
MQKVTIQEAEHLLQEIMSEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMSKYENALREKGLTYIAGIDEV
GRGPLAGPVVTAAVVLPEDFYIPGLNDSKKLSEAKRERFYDEIKAQAIAIGVGIVSPQVIDEINIYQATKQAMLDAVANL
SCTPQYLLIDAMKLPTPIPQTSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGI
LEEHRKSFAPIKDMIQK
>Mature_257_residues
MQKVTIQEAEHLLQEIMSEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMSKYENALREKGLTYIAGIDEV
GRGPLAGPVVTAAVVLPEDFYIPGLNDSKKLSEAKRERFYDEIKAQAIAIGVGIVSPQVIDEINIYQATKQAMLDAVANL
SCTPQYLLIDAMKLPTPIPQTSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGI
LEEHRKSFAPIKDMIQK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=53.3333333333333, Blast_Score=187, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17536263, Length=241, Percent_Identity=30.7053941908714, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 28966; Mature: 28966

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVTIQEAEHLLQEIMSEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMS
CCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYENALREKGLTYIAGIDEVGRGPLAGPVVTAAVVLPEDFYIPGLNDSKKLSEAKRERFY
HHHHHHHHCCCEEEECCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
DEIKAQAIAIGVGIVSPQVIDEINIYQATKQAMLDAVANLSCTPQYLLIDAMKLPTPIPQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
TSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGI
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
LEEHRKSFAPIKDMIQK
HHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MQKVTIQEAEHLLQEIMSEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMS
CCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYENALREKGLTYIAGIDEVGRGPLAGPVVTAAVVLPEDFYIPGLNDSKKLSEAKRERFY
HHHHHHHHCCCEEEECCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
DEIKAQAIAIGVGIVSPQVIDEINIYQATKQAMLDAVANLSCTPQYLLIDAMKLPTPIPQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
TSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGI
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
LEEHRKSFAPIKDMIQK
HHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA