Click here to switch to the map view.

The map label for this gene is opuE [H]

Identifier: 222097104

GI number: 222097104

Start: 3389286

End: 3390728

Strand: Reverse

Name: opuE [H]

Synonym: BCQ_3444

Alternate gene names: 222097104

Gene position: 3390728-3389286 (Counterclockwise)

Preceding gene: 222097105

Following gene: 222097103

Centisome position: 65.03

GC content: 36.94

Gene sequence:

>1443_bases
ATGGCAGGTATGTTGTATATCGGTTATTGGTCTTATAAGAAGACATCCGATTTATCAGATTATATGTTAGGCGGAAGGGG
ACTCGGTCCAGCAGTTACAGCTTTATCAGCTGGTGCTTCTGACATGAGTGGTTGGATGCTAATGGGATTACCTGGTGCGA
TGTATGCGACAGGGTTATCAAGTGTGTGGATTGCGATAGGTTTATTAATAGGTGCTTATGTAAACTATTTAATTCTTGCC
CCGCGTTTGCGAACGTATACAGAAGTGGCTAATGATTCAATTACGATTCCAGACTTTTTAGAGAATCGATTTAAAGATCG
TACGAAAATACTTCGTTTTGTCTCCGCTATCGTCATTTTAGTATTTTTCACATTTTATGCGTCGGCTGGTTTAGTATCAG
GTGGACGTTTGTTTGAAAATTCTTTCAACCTTGATTATAAAATTGGTTTATTTGTAACTGTTGGTGTCGTAGTTGCTTAT
ACACTATTCGGTGGTTTTTTAGCAGTAAGTTGGACCGACTTTGTGCAAGGGTGTATTATGTTTATTGCTCTCGTATTAGT
ACCAATTGTTGCTTTTACAGATGTCGGTGGTGTAACAGAAACATTCAATACGATTAAGCAAGTTGATGCATCGCATTTAG
ATATGTTTAAAGGGACCACAATTCTTGGTATTATTTCCTTTTTAGCATGGGGTCTTGGGTATTTTGGTCAACCACATATT
ATTGTCCGCTTTATGGCAATTACCTCTATTAAAGATTTGAAAACTTCTCGTAGAATCGGTATCGGTTGGATGACGATTTC
AATTATAGGTGCAATGCTTACTGGTCTAGTTGGTATTGCTTATTACGCTAAAAATAATGCGACATTACAAGATCCGGAAA
TGGTCTTTGTAACATTCTCAAATATTTTATTCCATCCGTACATTACTGGATTTTTATTATCAGCTATTTTGGCTTCGATT
ATGAGTAGTATTTCCTCGCAATTACTTGTTATTTCAAGTGCAGTAACGGAAGATTTCTATAAAACATTTTTCCGTCGTAA
CGCAAGTGATAAAGAACTTGTATTTATCGGTAGACTGTCAGTTTTAGTAGTAGCTATGATCGCAGTTGTTTTAGCGTATC
ATCCAAGTGATACAATTTTAACACTCGTCGGTTATGCTTGGGCAGGATTTGGTTCAGCATTCGGACCTGCCATTTTATTA
AGTTTATATTGGAAGAGAACGAACAAATGGGGCGTTCTTGCTGGGATGATTGTTGGTGCAGTAGTTGTAATTACTTGGGT
ACAAATTCCAAGTTTAAAAGCGACTATGTATGAAATGGTACCTGGATTCTTCTGTAGCTTATTAGCTGTGATTGTTATAA
GTTTATTAACGAAAGAACCAGTTAAAGCAATTCATCGTGAATTTAATGAGATGGAAGCAGTGCTGGAAGAAGAAACGAAA
TGA

Upstream 100 bases:

>100_bases
AAGAAAATAGAAAAAGTCATTTTTCGAAATTTTGAATGGCTTATGTAACTAAGGGAGAGATTTGGTGAAGATTGAGATTA
TGGTTTCGCTTGCTATTTAT

Downstream 100 bases:

>100_bases
TCAGCGAAGAGAGTAAACGAAACTGGCGTTTACTCTCTTTTTATTTAAAATGAAAATAAGGAACTTGTTGACAACTAAAA
GTAAGTAACTTATAATAAAT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIGLLIGAYVNYLILA
PRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAY
TLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFLAWGLGYFGQPHI
IVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPYITGFLLSAILASI
MSSISSQLLVISSAVTEDFYKTFFRRNASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILL
SLYWKRTNKWGVLAGMIVGAVVVITWVQIPSLKATMYEMVPGFFCSLLAVIVISLLTKEPVKAIHREFNEMEAVLEEETK

Sequences:

>Translated_480_residues
MAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIGLLIGAYVNYLILA
PRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAY
TLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFLAWGLGYFGQPHI
IVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPYITGFLLSAILASI
MSSISSQLLVISSAVTEDFYKTFFRRNASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILL
SLYWKRTNKWGVLAGMIVGAVVVITWVQIPSLKATMYEMVPGFFCSLLAVIVISLLTKEPVKAIHREFNEMEAVLEEETK
>Mature_479_residues
AGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIGLLIGAYVNYLILAP
RLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYT
LFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFLAWGLGYFGQPHII
VRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPYITGFLLSAILASIM
SSISSQLLVISSAVTEDFYKTFFRRNASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLS
LYWKRTNKWGVLAGMIVGAVVVITWVQIPSLKATMYEMVPGFFCSLLAVIVISLLTKEPVKAIHREFNEMEAVLEEETK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=485, Percent_Identity=55.4639175257732, Blast_Score=536, Evalue=1e-152,
Organism=Homo sapiens, GI11141885, Length=457, Percent_Identity=24.945295404814, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI14140236, Length=530, Percent_Identity=23.7735849056604, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI110835708, Length=539, Percent_Identity=22.6345083487941, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI109659836, Length=385, Percent_Identity=22.3376623376623, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4507031, Length=443, Percent_Identity=24.8306997742664, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI109659839, Length=401, Percent_Identity=21.6957605985037, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI206597487, Length=464, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI206597483, Length=464, Percent_Identity=22.198275862069, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1787251, Length=475, Percent_Identity=50.3157894736842, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI87082237, Length=432, Percent_Identity=30.3240740740741, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790503, Length=448, Percent_Identity=27.2321428571429, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790113, Length=391, Percent_Identity=24.2966751918159, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539284, Length=450, Percent_Identity=25.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533094, Length=384, Percent_Identity=21.6145833333333, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221459584, Length=372, Percent_Identity=24.1935483870968, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648033, Length=441, Percent_Identity=22.6757369614512, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356865, Length=441, Percent_Identity=22.6757369614512, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640370, Length=358, Percent_Identity=23.7430167597765, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24645928, Length=368, Percent_Identity=24.7282608695652, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24651739, Length=449, Percent_Identity=24.2761692650334, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362918, Length=297, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24651741, Length=458, Percent_Identity=24.6724890829694, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI221459588, Length=380, Percent_Identity=23.1578947368421, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221459586, Length=385, Percent_Identity=22.0779220779221, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52528; Mature: 52396

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLS
CCCEEEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
SVWIAIGLLIGAYVNYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVIL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIM
HHHHHHHHCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFLAWGLGYFGQPHI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHH
IVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHH
NILFHPYITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRNASDKELVFIGRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHH
VLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLSLYWKRTNKWGVLAGMIVGA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVVITWVQIPSLKATMYEMVPGFFCSLLAVIVISLLTKEPVKAIHREFNEMEAVLEEETK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLS
CCEEEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
SVWIAIGLLIGAYVNYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVIL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIM
HHHHHHHHCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFLAWGLGYFGQPHI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHH
IVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHH
NILFHPYITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRNASDKELVFIGRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHH
VLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLSLYWKRTNKWGVLAGMIVGA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVVITWVQIPSLKATMYEMVPGFFCSLLAVIVISLLTKEPVKAIHREFNEMEAVLEEETK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]