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The map label for this gene is oppA [H]

Identifier: 222097039

GI number: 222097039

Start: 3326370

End: 3328076

Strand: Reverse

Name: oppA [H]

Synonym: BCQ_3379

Alternate gene names: 222097039

Gene position: 3328076-3326370 (Counterclockwise)

Preceding gene: 222097040

Following gene: 222097038

Centisome position: 63.83

GC content: 35.91

Gene sequence:

>1707_bases
ATGAAGAAAAAGATGAAAAAGTTCACGGCAGTTGTAGCGCCAGTTTTAGCGATGAGTATGGCGTTGACAGCGTGTTCTGG
ATCTGGTGGGGAGAAGAAAACAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAATCTGAAATTAAATACGCAGCGA
AACAAGTGTTAAATCGTACAGAGAATCAAGAAATTCCGACGATGGATACGTCTAAATCTACTGATACGTTAGGGTCACAA
ATTTTAGGGAATACAATGGAAGGATTATATCGTCTTGATAAAGATAATAAGCCAATCCCAGCTGCAGCGGAATCTAGTAC
GAAGAGCGAGGATGGCAAAAAATATACATTTAAATTACGGAAAGATGCAAAATGGTCAAATGGTGATCCAGTAACAGCGA
AAGATTTTGTATTTGCATGGCAGCGCTTACTGGATAAAAATACAGCGTCAGAATACGCATTTATTGCTTACTATATTAAA
AACGCAGAGGCAATTAATAAAGGTGAAAAGCCACTAACAGATTTAGGGGCAAAAGCGGTAGATGATTATACGCTAGAAGT
AGAATTAGAAAAACCGGTGCCGTATTTCTTGAATTTAATGGCATTCCCATCGTACTATCCTTTAAATGAAAAGTTCGTAA
AAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACAT
GAACAAGGATGGCAGCTAAAGAAAAATGATAAGTATTGGGATAAGAAGACTGTGAAATTAGAAGAAATTAACTATAGTGT
AGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACACTATTATCAGGAGAATTTGTTG
ATAAATATAAATCGAACAAAGAAGAGTATGGTGAGTACTCAGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAACGT
AATGGACAAGACACACCGTTAAAGAGTAAAAAACTACGGGAAGCAATCGCATTATCAATTGATAAAAAAGGTTTAGCAAA
CGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTGGTTCCAAAAGGGCTTGCGACAGGACCTGATGGTAAAGATT
ACCAAGATACATTTAAAAATGGTCTGAAATATAACCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAGCTT
GGTAAAGATCAAGTTACAATTGAATTACTAAGCTATGATGATGGAACAGCGAAAAAAATTGCTGACTACTTTAAAGATCA
AATTGAGAAAAACTTAAAAGGTGTAACGGTTAATACGAAAATTCAGCCGTTCAAACAAAAACTAAAATTAGAATCGGCAC
AAGATTATGAAATTTCATTTGCTGGTTGGAGTCCGGACTATGCGGATCCAATGACATTTATTGATATGTTCGAATCGAAG
AGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTGAAAAAAGCAGGGAATGAATTAATGTCTGA
TGCGAAGAAGCGTTGGGAAACGTTAGGGAAAGCAGAAAAATTATTACTTGAAGAAGATGCAGGAATGGTACCTTTATATC
AAACAGGTAGAGCATATGTAATGAAACCGAATGTAAAAGGCATTGTGAAACATAACATTAGTCCAGAGTATAGCTTTAAG
TGGGCTTATGTAACGGAAGGGAAATAA

Upstream 100 bases:

>100_bases
AAAATTAGTAGAATATTAATTAATAAGAATTTTCTGATAACTAGTTATTAGAAAATCTCTCAAAATGAGTTTCGTACATA
TAATTGGGGAGGGTATAAAA

Downstream 100 bases:

>100_bases
TAAAAAGGAGCGTGTTAAAAGATAATTACCTTTAACACGCTCTTTTTCTATTAAATTAAATAGAGAAGAATAAGAAAAGT
GGTACTGACATATAGTAGAT

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKTTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDTSKSTDTLGSQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLLDKNTASEYAFIAYYIK
NAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKR
NGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKEL
GKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEISFAGWSPDYADPMTFIDMFESK
SPYNQMSYSNPKYDEMVKKAGNELMSDAKKRWETLGKAEKLLLEEDAGMVPLYQTGRAYVMKPNVKGIVKHNISPEYSFK
WAYVTEGK

Sequences:

>Translated_568_residues
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKTTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDTSKSTDTLGSQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLLDKNTASEYAFIAYYIK
NAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKR
NGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKEL
GKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEISFAGWSPDYADPMTFIDMFESK
SPYNQMSYSNPKYDEMVKKAGNELMSDAKKRWETLGKAEKLLLEEDAGMVPLYQTGRAYVMKPNVKGIVKHNISPEYSFK
WAYVTEGK
>Mature_568_residues
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKTTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDTSKSTDTLGSQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLLDKNTASEYAFIAYYIK
NAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKR
NGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKEL
GKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEISFAGWSPDYADPMTFIDMFESK
SPYNQMSYSNPKYDEMVKKAGNELMSDAKKRWETLGKAEKLLLEEDAGMVPLYQTGRAYVMKPNVKGIVKHNISPEYSFK
WAYVTEGK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=511, Percent_Identity=31.5068493150685, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789397, Length=509, Percent_Identity=29.4695481335953, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI87081878, Length=536, Percent_Identity=27.4253731343284, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787762, Length=455, Percent_Identity=28.1318681318681, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1787052, Length=498, Percent_Identity=23.8955823293173, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789887, Length=336, Percent_Identity=26.4880952380952, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789966, Length=483, Percent_Identity=22.9813664596273, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63960; Mature: 63960

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKTTTTSSGGGEEKKSEIKYAAKQVLNRT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ENQEIPTMDTSKSTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVFAWQRLLDKNTASEYAFIAYYIKNAEAINKGEKPLTDLGAKAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCHHHHCCHHC
DDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEEEECCC
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNK
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEECCCEEEEEECCHHHHHHHCCH
EEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQL
HHHCCHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEECCCCHHHHHH
VPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKI
CCCCCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHH
ADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEISFAGWSPDYADPMTFIDMFESK
HHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCC
SPYNQMSYSNPKYDEMVKKAGNELMSDAKKRWETLGKAEKLLLEEDAGMVPLYQTGRAYV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEE
MKPNVKGIVKHNISPEYSFKWAYVTEGK
ECCCCCCCEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKTTTTSSGGGEEKKSEIKYAAKQVLNRT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ENQEIPTMDTSKSTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVFAWQRLLDKNTASEYAFIAYYIKNAEAINKGEKPLTDLGAKAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCHHHHCCHHC
DDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEEEECCC
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNK
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEECCCEEEEEECCHHHHHHHCCH
EEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQL
HHHCCHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEECCCCHHHHHH
VPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKI
CCCCCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHH
ADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEISFAGWSPDYADPMTFIDMFESK
HHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCC
SPYNQMSYSNPKYDEMVKKAGNELMSDAKKRWETLGKAEKLLLEEDAGMVPLYQTGRAYV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEE
MKPNVKGIVKHNISPEYSFKWAYVTEGK
ECCCCCCCEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]