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The map label for this gene is ykfC [H]

Identifier: 222096346

GI number: 222096346

Start: 2628016

End: 2629017

Strand: Reverse

Name: ykfC [H]

Synonym: BCQ_2686

Alternate gene names: 222096346

Gene position: 2629017-2628016 (Counterclockwise)

Preceding gene: 222096347

Following gene: 222096345

Centisome position: 50.42

GC content: 39.22

Gene sequence:

>1002_bases
ATGAAAAAAGTAGGAACTGCATTTTTAACAACTTTATTTATATTTTCATCGTTTACATCAGCAAATGCTGAGGAAAAGAA
AGATAGTAAGGCGTTTATCGATGTATCTGCTGCAACACTTTGGACCACGCCTGATTCATTACGGCCAATTGATGCACCAA
GTGCTACAAATCCAGTTGATTTGTGGAAGTGGACGAAATCGATGACGCTTGATGAGAAACTTTGGTTAACAAGTGCAAAT
AAATTAGAAACGCAAGCGTTATTAGGCCAAGAAGTAACAGTTGTAGATAAAAAAGGGGATTGGGTGAAAGTGTTAGTCCA
TGGTCAGCCAACACCTCGAAATGAAGAAGGCTACCCAGGATGGATGCCTGAAAAACAGTTAACATATAATCAAGAATTTG
CAGATAAAACAAATAAACCTTTCGTATTAGTAACGAAACCGACAGCCATTTTATATATAAATCCTTCTGAAAAACATAAA
TCGCTTGAAGTGAGCTATAATACGCGACTGCCGCTACTTAGTGAAGATACGATTTCATACCGCGTGTTGCTACCAAATGG
GCAGAAAGCTTGGCTACGAAAAAATGATGGAACGGTTTACCGTTCTCAAAATGATATTCCAACCCCGACGGCCGATGATC
TAATAAACACAGGGAAAATGTTTTTAGGATTACCGTACATTTGGGCTGGTACAAGTGGCTTCGGATTTGATTGCTCTGGA
TTTACACATACGATTTATAAATCGCACGGTATTACAATTCCACGAGATTCTGGTCCGCAATCGAGAAATGGAGTTGCAGT
TGATAAAGAACATTTACAAAAAGGAGATTTAATCTTCTTTGCACATGATCAAGGGAAAGGCAGTGTTCATCATGTGGGGA
TGTACATTGGTGATGGAAATATGATTCACTCACCGAGAGCTGAAAGATCGGTAGAGATTATACCGCTAAATACACCAGGA
TATATAGAAGAATACGCTGGCGCCCGTCGTTACTTACCTTAA

Upstream 100 bases:

>100_bases
AGGAATGACTTATTCTGGATCAAAGGTAAACCTTCAATCGACAGTGAATAGTAAATCTTAGGAGAAGAGACTAGTAAATT
ATCTTTTAAGGGGGATATGT

Downstream 100 bases:

>100_bases
AAAATAGAGGGGGTTTTCAAATGAAAAAAGTTGTTCGTTACTCATTAGTTAGTACTCTATTCGTTTCTTCAATTTTAGTT
GGCTGCGCAAAAGAAAAAAC

Product: cell wall-associated hydrolase

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVDLWKWTKSMTLDEKLWLTSAN
KLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPGWMPEKQLTYNQEFADKTNKPFVLVTKPTAILYINPSEKHK
SLEVSYNTRLPLLSEDTISYRVLLPNGQKAWLRKNDGTVYRSQNDIPTPTADDLINTGKMFLGLPYIWAGTSGFGFDCSG
FTHTIYKSHGITIPRDSGPQSRNGVAVDKEHLQKGDLIFFAHDQGKGSVHHVGMYIGDGNMIHSPRAERSVEIIPLNTPG
YIEEYAGARRYLP

Sequences:

>Translated_333_residues
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVDLWKWTKSMTLDEKLWLTSAN
KLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPGWMPEKQLTYNQEFADKTNKPFVLVTKPTAILYINPSEKHK
SLEVSYNTRLPLLSEDTISYRVLLPNGQKAWLRKNDGTVYRSQNDIPTPTADDLINTGKMFLGLPYIWAGTSGFGFDCSG
FTHTIYKSHGITIPRDSGPQSRNGVAVDKEHLQKGDLIFFAHDQGKGSVHHVGMYIGDGNMIHSPRAERSVEIIPLNTPG
YIEEYAGARRYLP
>Mature_333_residues
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVDLWKWTKSMTLDEKLWLTSAN
KLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPGWMPEKQLTYNQEFADKTNKPFVLVTKPTAILYINPSEKHK
SLEVSYNTRLPLLSEDTISYRVLLPNGQKAWLRKNDGTVYRSQNDIPTPTADDLINTGKMFLGLPYIWAGTSGFGFDCSG
FTHTIYKSHGITIPRDSGPQSRNGVAVDKEHLQKGDLIFFAHDQGKGSVHHVGMYIGDGNMIHSPRAERSVEIIPLNTPG
YIEEYAGARRYLP

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=115, Percent_Identity=39.1304347826087, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786421, Length=115, Percent_Identity=38.2608695652174, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788501, Length=112, Percent_Identity=33.9285714285714, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 37193; Mature: 37193

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCHH
LWKWTKSMTLDEKLWLTSANKLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPG
HHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCC
WMPEKQLTYNQEFADKTNKPFVLVTKPTAILYINPSEKHKSLEVSYNTRLPLLSEDTISY
CCCCCCCCCCHHHHHCCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEECCCEEE
RVLLPNGQKAWLRKNDGTVYRSQNDIPTPTADDLINTGKMFLGLPYIWAGTSGFGFDCSG
EEEECCCCEEEEECCCCEEEECCCCCCCCCHHHHHHCCHHHEECCEEEECCCCCCCCCCC
FTHTIYKSHGITIPRDSGPQSRNGVAVDKEHLQKGDLIFFAHDQGKGSVHHVGMYIGDGN
CEEEEEECCCEECCCCCCCCCCCCEEECHHHCCCCCEEEEEECCCCCCEEEEEEEEECCC
MIHSPRAERSVEIIPLNTPGYIEEYAGARRYLP
EEECCCCCCCEEEEECCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCHH
LWKWTKSMTLDEKLWLTSANKLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPG
HHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCC
WMPEKQLTYNQEFADKTNKPFVLVTKPTAILYINPSEKHKSLEVSYNTRLPLLSEDTISY
CCCCCCCCCCHHHHHCCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEECCCEEE
RVLLPNGQKAWLRKNDGTVYRSQNDIPTPTADDLINTGKMFLGLPYIWAGTSGFGFDCSG
EEEECCCCEEEEECCCCEEEECCCCCCCCCHHHHHHCCHHHEECCEEEECCCCCCCCCCC
FTHTIYKSHGITIPRDSGPQSRNGVAVDKEHLQKGDLIFFAHDQGKGSVHHVGMYIGDGN
CEEEEEECCCEECCCCCCCCCCCCEEECHHHCCCCCEEEEEECCCCCCEEEEEEEEECCC
MIHSPRAERSVEIIPLNTPGYIEEYAGARRYLP
EEECCCCCCCEEEEECCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]