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The map label for this gene is oppA [H]

Identifier: 222096345

GI number: 222096345

Start: 2626373

End: 2627995

Strand: Reverse

Name: oppA [H]

Synonym: BCQ_2685

Alternate gene names: 222096345

Gene position: 2627995-2626373 (Counterclockwise)

Preceding gene: 222096346

Following gene: 222096344

Centisome position: 50.4

GC content: 34.44

Gene sequence:

>1623_bases
ATGAAAAAAGTTGTTCGTTACTCATTAGTTAGTACTCTATTCGTTTCTTCAATTTTAGTTGGCTGCGCAAAAGAAAAAAC
AACGACAAAGCCGAAAGATGAGAAGAAAGTATTACAGTTACTAGAAACGGGTGAAATTCCGTCATTAAATTCAGGGAAAG
TAACAGATGCAGTATCATTTAACGTTTTAAATAACGTCATGGAAGGATTATTCCGCTTATCGAAAAACGATGAAGTGATT
GAGGCTGGAGCACAAAAATATGAAGTGAGTAAAGATGGGAAAACATATACATTCCACTTGCGTGATGCGAAGTGGTCTAA
CGGAGATCCAGTAACAGCGCAAGATTACGTATATGCTTGGAAGCAGCTTATTAATCCGGATACTGCTTCTCAATATGCAT
ACATTGCGTACGATGTGAAAAATGCGGAGAAAATAAATAAGAAACAACTAGGGTTAGAGGAATTAGGAGTAAAAGCGAAG
GATGATAAAACGTTCGTTGTAGAGTTGGAACATCCTGTACCGTATTTTACGAAACTACTTATTTTACCGTCCTTCTATCC
AATTAACGAGAAATATGCGAAAGAGCAAGGCGATAAATATGGATTAGAAGCAAATAAAACGGTATATAATGGGCCATTTA
CATTATCTGATTGGAAGCATGAAGCTAGTTTTACGATGAAGAAAAACGATAAGTATTGGGATAAAAAAGAAGTGAAGCTA
GATGAAGTAAACTATCAAATTGTTAAAGAAATTTCAACTGCGGTAAATTTATATGAAACAGATAAAGTTGATAGAGCTGT
CATTTCTACAGAATTCGTAGATAAATATAAAAATAATAAAGAGCTAAAACAATATACAGATCCAGTTATGTACTTCTTCC
GATTTAATGAAAATGTGCCGATTCTTAAAAATAAAAATGCACGTCTTGCACTAAGTACAGTGTTTGATAAGAAAGGACTT
GCGACTTCATTTTTAAATGATGGATCGGTTGCTGCAAACTATTACGTGCCAAAAGGATTTTTAAAAGGTCCAGATAAAAA
AGATTTTAGAAGTACAGCAGGTGAGTTTAATAAAACGGATGTAAAACAGGCGAAAGAATATTGGGAAAAGGCGAAGCAAG
AAACAGGTACAAATGAAGTAACGCTTGAGTTATTAAACTATGATTTAGAAAACTTCAAAAAAGTAGGAGAATATATTAAA
GAGCAGCTTGAGAAAAACTTACCTGGCTTAAAGGTAAATGTGAAATTACAACCACACACGCAAAAATTAGCGCTAGAGAA
GAAGAAAGAATATGAAATGTCGTTATCACGCTGGTTACCAGATTATCCAGATCCAATGACTTACTTAGAAGTATTCCTTT
CTGGAAGCAGTGTAAACAATACTGAATATGCAAATCCAGAATATGATGCGTTAATTAAGAAGATTAAAACAGAATTAGGT
AATGATGAAAAAAACCGTTGGAAAGCATTGCAAGATGCTGAGAAGATGTTGCTGGATGATGCGGTAATTGCTCCAGTATT
CCAGCGCGGATTATCTTACTTACAAAAGCCGTATGTGAAAGATTTATACGTACATCAATTTGGACCAGCAACAAGTTTAA
AGTGGGCAGATGTACAAAAATAA

Upstream 100 bases:

>100_bases
AAAGATCGGTAGAGATTATACCGCTAAATACACCAGGATATATAGAAGAATACGCTGGCGCCCGTCGTTACTTACCTTAA
AAAATAGAGGGGGTTTTCAA

Downstream 100 bases:

>100_bases
AGGATGAGAAAACAGACTCCACGTTTGTGGTAGTCTGTTTTTTAGTTAAAGTCGGGTAAATAGTACATGGTATAATTTAC
CATTATGTATAAAATTTTGG

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKKVVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSFNVLNNVMEGLFRLSKNDEVI
EAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGLEELGVKAK
DDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKTVYNGPFTLSDWKHEASFTMKKNDKYWDKKEVKL
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGL
ATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGTNEVTLELLNYDLENFKKVGEYIK
EQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
NDEKNRWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK

Sequences:

>Translated_540_residues
MKKVVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSFNVLNNVMEGLFRLSKNDEVI
EAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGLEELGVKAK
DDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKTVYNGPFTLSDWKHEASFTMKKNDKYWDKKEVKL
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGL
ATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGTNEVTLELLNYDLENFKKVGEYIK
EQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
NDEKNRWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
>Mature_540_residues
MKKVVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSFNVLNNVMEGLFRLSKNDEVI
EAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGLEELGVKAK
DDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKTVYNGPFTLSDWKHEASFTMKKNDKYWDKKEVKL
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGL
ATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGTNEVTLELLNYDLENFKKVGEYIK
EQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
NDEKNRWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=29.5880149812734, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=29.3457943925234, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789397, Length=489, Percent_Identity=28.8343558282209, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1787762, Length=439, Percent_Identity=23.0068337129841, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789966, Length=497, Percent_Identity=22.9376257545272, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62065; Mature: 62065

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
NVLNNVMEGLFRLSKNDEVIEAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAW
HHHHHHHHHHHHCCCCCHHHHHCCHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
KQLINPDTASQYAYIAYDVKNAEKINKKQLGLEELGVKAKDDKTFVVELEHPVPYFTKLL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCHHHHHHH
ILPSFYPINEKYAKEQGDKYGLEANKTVYNGPFTLSDWKHEASFTMKKNDKYWDKKEVKL
HHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEECCCCCCCCCEEECCCCCCCCCCCCCH
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCC
ILKNKNARLALSTVFDKKGLATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTD
EEECCCCEEEEEEHHCCCCCHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHH
VKQAKEYWEKAKQETGTNEVTLELLNYDLENFKKVGEYIKEQLEKNLPGLKVNVKLQPHT
HHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
QKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
NDEKNRWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
NVLNNVMEGLFRLSKNDEVIEAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAW
HHHHHHHHHHHHCCCCCHHHHHCCHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
KQLINPDTASQYAYIAYDVKNAEKINKKQLGLEELGVKAKDDKTFVVELEHPVPYFTKLL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCHHHHHHH
ILPSFYPINEKYAKEQGDKYGLEANKTVYNGPFTLSDWKHEASFTMKKNDKYWDKKEVKL
HHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEECCCCCCCCCEEECCCCCCCCCCCCCH
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCC
ILKNKNARLALSTVFDKKGLATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTD
EEECCCCEEEEEEHHCCCCCHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHH
VKQAKEYWEKAKQETGTNEVTLELLNYDLENFKKVGEYIKEQLEKNLPGLKVNVKLQPHT
HHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
QKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
NDEKNRWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]