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The map label for this gene is rhtB [H]

Identifier: 222095942

GI number: 222095942

Start: 2245116

End: 2245748

Strand: Reverse

Name: rhtB [H]

Synonym: BCQ_2282

Alternate gene names: 222095942

Gene position: 2245748-2245116 (Counterclockwise)

Preceding gene: 222095943

Following gene: 222095938

Centisome position: 43.07

GC content: 31.28

Gene sequence:

>633_bases
ATGATTGAAAATTATTTTCTTTTCATCATTATGTCTATTTGTCTTATTATTTTACCTGGTCCTGATACTGCAATGGCTAC
GAAAAACACATTAGTTGCTGGTAAGGTAGGTGGGGTGAAAACAGTGTTCGGTACATGTGTTGCACTATTAATTCATACTT
TAGCAGCAGTAATCGGACTTTCCGCACTTATTGTAAAGTCAGCTCTTTTATTTTCTATTTTCAAGTACGTTGGAGCTCTA
TATTTAATTTATATTGGTATTAAAGCCCTTTTAGCAGTAAGAAATAAAGAAGGCGTCAATACGAACGATATATCTTTGAA
TAACGCAAACGAGAATACTTCTTGCTTTCGCCAAGGGTTTCTTACAAATTTATTAAACCCTAAAATCGCAGTCTTCTTTT
TAACATTTTTACCGCAATTTTTAAATCCAAATCATAATACGTTTATCCAACTTCTTATAATGGGCCTTACTTACCTAGTT
TTAACAGTTATTTGGTTCGCTTTTTATATATTTTTAATTGATAAAATTAGTGCTTTTATGAAAAAACCGAAGACGCAACG
TTATATTCAAGGGTTAACAGGAATTGTCTTAATTGGTTTTGGTATTAAACTCGCTTTTGAAAAAAGCAATTAA

Upstream 100 bases:

>100_bases
TATTTAATAAATTTTAATAAAATTTAATTTTATTAAATCGAATTATGTGCTACACTATTTTTAATTAAATATTCTAAAAA
TAAAAGAAGGAGGAGCGACT

Downstream 100 bases:

>100_bases
CAAGAAGAAAACCACCACTATGAATAGTGGTGGTTTTCTTTTAATAACTGATTTGGTTGTCTACATTATATTAAAACCAG
CGCTCAGTTACAACTTTTTT

Product: homoserine/threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCVALLIHTLAAVIGLSALIVKSALLFSIFKYVGAL
YLIYIGIKALLAVRNKEGVNTNDISLNNANENTSCFRQGFLTNLLNPKIAVFFLTFLPQFLNPNHNTFIQLLIMGLTYLV
LTVIWFAFYIFLIDKISAFMKKPKTQRYIQGLTGIVLIGFGIKLAFEKSN

Sequences:

>Translated_210_residues
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCVALLIHTLAAVIGLSALIVKSALLFSIFKYVGAL
YLIYIGIKALLAVRNKEGVNTNDISLNNANENTSCFRQGFLTNLLNPKIAVFFLTFLPQFLNPNHNTFIQLLIMGLTYLV
LTVIWFAFYIFLIDKISAFMKKPKTQRYIQGLTGIVLIGFGIKLAFEKSN
>Mature_210_residues
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCVALLIHTLAAVIGLSALIVKSALLFSIFKYVGAL
YLIYIGIKALLAVRNKEGVNTNDISLNNANENTSCFRQGFLTNLLNPKIAVFFLTFLPQFLNPNHNTFIQLLIMGLTYLV
LTVIWFAFYIFLIDKISAFMKKPKTQRYIQGLTGIVLIGFGIKLAFEKSN

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=207, Percent_Identity=28.0193236714976, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1788099, Length=208, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI48994978, Length=199, Percent_Identity=26.6331658291457, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23226; Mature: 23226

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCVALLIHTLAAVIGL
CCCHHHHHHHHHHHHHEECCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
SALIVKSALLFSIFKYVGALYLIYIGIKALLAVRNKEGVNTNDISLNNANENTSCFRQGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHH
LTNLLNPKIAVFFLTFLPQFLNPNHNTFIQLLIMGLTYLVLTVIWFAFYIFLIDKISAFM
HHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKPKTQRYIQGLTGIVLIGFGIKLAFEKSN
HCCCHHHHHHHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCVALLIHTLAAVIGL
CCCHHHHHHHHHHHHHEECCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
SALIVKSALLFSIFKYVGALYLIYIGIKALLAVRNKEGVNTNDISLNNANENTSCFRQGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHH
LTNLLNPKIAVFFLTFLPQFLNPNHNTFIQLLIMGLTYLVLTVIWFAFYIFLIDKISAFM
HHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKPKTQRYIQGLTGIVLIGFGIKLAFEKSN
HCCCHHHHHHHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9308178 [H]